view article
Figure 5
Butyryl-CoA competitively inhibits acetylation by human Gcn5. (a) Steady-state kinetic analysis comparing acetylation and butyrylation by human Gcn5 using a fixed concentration of 300 µM H3 peptide. By fitting this curve to the Michaelis–Menten equation, we obtain Km = 1.05 ± 0.02 µM and kcat = 0.91 ± 0.09 s−1. (b) Acetylation kinetics monitored in the presence of increasing concentrations of butyryl-CoA (0–0.3 mM). Data were globally fitted to a competitive-inhibition model.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 72| Part 7| July 2016| Pages 841-848
doi:10.1107/S2059798316007907
Follow Acta Cryst. D
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS