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Figure 5
Sequence alignment of PsPI-PLC with SanPI-PLC and rat PI-PLC, with calcium-binding residues (putative calcium-binding residues for SanPI-PLC) and catalytic histidines outlined in green. Secondary-structure elements are shown as symbols for PsPI-PLC and as background colours for the other two sequences. In both types of presentation, helices are red, 310-helices yellow and β-­strands violet. ClustalX (Larkin et al., 2007BB33) and the structure superpositions described in Table 2[link] were used for the initial alignment of X-boxes and manual adjustment, respectively. The alignment for Y-boxes was obtained in a similar manner but required more substantial structure-based manual correction. The graphical representation of alignment shown here was prepared using ALINE (Bond & Schüttelkopf, 2009BB8)

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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