Figure 5
Sequence alignment of PsPI-PLC with SanPI-PLC and rat PI-PLC, with calcium-binding residues (putative calcium-binding residues for SanPI-PLC) and catalytic histidines outlined in green. Secondary-structure elements are shown as symbols for PsPI-PLC and as background colours for the other two sequences. In both types of presentation, helices are red, 310-helices yellow and β-strands violet. ClustalX (Larkin et al., 2007) and the structure superpositions described in Table 2 were used for the initial alignment of X-boxes and manual adjustment, respectively. The alignment for Y-boxes was obtained in a similar manner but required more substantial structure-based manual correction. The graphical representation of alignment shown here was prepared using ALINE (Bond & Schüttelkopf, 2009) |