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Figure 5
Crystal structure of Aae Hfq in the apo form, with head→tail stacking of hexameric rings. The apo form of Aae Hfq crystallized in space group P1 as a dodecameric assembly of hexamers stacked in a proximal→distal orientation in the lattice. Ribbon diagrams of the final, refined structure are shown here from perpendicular viewpoints. The proximal-exposed (PE) hexamer is colored blue and cyan, and subunits in the distal-exposed (DE) hexamer are colored alternatingly yellow and orange. Co-crystallizing molecules of MPD (gray C atoms) and Gnd (green C atoms) are shown in ball-and-stick representation, and Cl ions are rendered as yellow spheres scaled to the van der Waals radius. Note that many of the Gnd cations and Cl anions are coplanar, where they form a `salty' layer at the ring interface (this is most clearly seen in the transverse view). Contacts between hexamers are mediated by the N-termini of the DE hexamer (top) and the loop L2/strand β2 regions of the PE hexamer (bottom); the approximate location of one of the lateral rim RNA-binding sites is labeled on the DE ring.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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