The crystal structure of the Yersinia pestis iron chaperone YiuA reveals a basic triad binding motif for the chelated metal

Radka, C.D.; Chen, D.; DeLucas, L.J.; Aller, S.G.

doi:10.1107/S2059798317015236

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 8
Loci acclimation in vitro. E. coli expressing the Yiu transporter require a threefold greater time to acclimate under nutrient-limiting conditions than E. coli expressing the Yfe transporter. (a, b, c) Periplasmic fractions of E. coli constructs growing in various in vitro media conditions. Black arrows denote the positions of electrophoretic migration for YfeA (b) and YiuA (c). Lanes 1–4 represent time points 3, 5, 7 and 10 h from M9 minimal medium base conditions. Lanes 5–8 represent time points 3, 5, 7 and 10 h from M9 minimal medium with 5 µM Fe₂(SO₄)₃ supplementation. Lanes 9–12 represent time points 3, 5, 7 and 10 h from M9 minimal medium with 1 mM EDDA supplementation. Molecular-weight markers are labeled in kDa. (d, e, f) Growth curves for the pET-22b construct (d), pYFE3 construct (e) and pYIU3 construct (f). Error bars represent the standard deviation in OD₆₀₀ from experiments performed in triplicate.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 73| Part 11| November 2017| Pages 921-939

https://doi.org/10.1107/S2059798317015236

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