Supporting information
Crystallographic Information File (CIF) https://doi.org/10.1107/S160053680703303X/rz2154sup1.cif | |
Structure factor file (CIF format) https://doi.org/10.1107/S160053680703303X/rz2154Isup2.hkl |
CCDC reference: 657754
Key indicators
- Single-crystal X-ray study
- T = 100 K
- Mean (C-C) = 0.002 Å
- R factor = 0.033
- wR factor = 0.084
- Data-to-parameter ratio = 9.8
checkCIF/PLATON results
No syntax errors found
Alert level C ABSTM02_ALERT_3_C The ratio of expected to reported Tmax/Tmin(RR') is < 0.90 Tmin and Tmax reported: 0.783 0.954 Tmin(prime) and Tmax expected: 0.933 0.954 RR(prime) = 0.840 Please check that your absorption correction is appropriate. PLAT061_ALERT_3_C Tmax/Tmin Range Test RR' too Large ............. 0.84 PLAT230_ALERT_2_C Hirshfeld Test Diff for N2A - N3A .. 6.14 su PLAT720_ALERT_4_C Number of Unusual/Non-Standard Label(s) ........ 12
Alert level G REFLT03_ALERT_4_G Please check that the estimate of the number of Friedel pairs is correct. If it is not, please give the correct count in the _publ_section_exptl_refinement section of the submitted CIF. From the CIF: _diffrn_reflns_theta_max 28.28 From the CIF: _reflns_number_total 3960 Count of symmetry unique reflns 3959 Completeness (_total/calc) 100.03% TEST3: Check Friedels for noncentro structure Estimate of Friedel pairs measured 1 Fraction of Friedel pairs measured 0.000 Are heavy atom types Z>Si present no PLAT791_ALERT_1_G Confirm the Absolute Configuration of C1A = . S PLAT791_ALERT_1_G Confirm the Absolute Configuration of C1B = . S PLAT791_ALERT_1_G Confirm the Absolute Configuration of C2A = . S PLAT791_ALERT_1_G Confirm the Absolute Configuration of C2B = . S PLAT791_ALERT_1_G Confirm the Absolute Configuration of C3A = . S PLAT791_ALERT_1_G Confirm the Absolute Configuration of C3B = . S PLAT791_ALERT_1_G Confirm the Absolute Configuration of C4A = . R PLAT791_ALERT_1_G Confirm the Absolute Configuration of C4B = . R PLAT791_ALERT_1_G Confirm the Absolute Configuration of C5A = . S PLAT791_ALERT_1_G Confirm the Absolute Configuration of C5B = . S
0 ALERT level A = In general: serious problem 0 ALERT level B = Potentially serious problem 4 ALERT level C = Check and explain 11 ALERT level G = General alerts; check 10 ALERT type 1 CIF construction/syntax error, inconsistent or missing data 1 ALERT type 2 Indicator that the structure model may be wrong or deficient 2 ALERT type 3 Indicator that the structure quality may be low 2 ALERT type 4 Improvement, methodology, query or suggestion 0 ALERT type 5 Informative message, check
Under a nitrogen atmosphere, ceric ammonium nitrate (10.84 g, 19.78 mmol) and sodium azide (1.35 g, 20.76 mmol) were placed in a flame-dried flask equipped with a magnetic stir bar and rubber septum. The reaction vessel was cooled to 258 K (-15 °C) using a dry ice ethylene glycol slush bath. 3,4-Di-O-acetyl-L-fucal (2.13 g, 9.94 mmol) was dissolved in freshly distilled acetone (50 ml) and transferred via cannula to the reaction vessel while maintaining 258 K. Overnight stirring and monitoring by TLC (3:1 petroleum ether-EtOAc) showed consumption of the starting material and the appearance of spots corresponding to the α- and β-anomers of 3,4-di-O-acetyl-2-azidodeoxy-1-O-nitro-L-fucose and the α- and β-anomers of 3,4-di-O-acetyl-2-azidodeoxy-L-fucose. The reaction mixture was diluted with diethyl ether (40 ml) and washed with H2O (3 × 20 ml). The organic layer was dried over MgSO4, filtered, and concentrated under reduced pressure. Column chromatography on silica gel (2:1 hexane-ethyl acetate as eluent) provided the mixture of the α- and β-anomers of 3,4-di-O-acetyl-2-azidodeoxy-1-O-nitro-L-fucose in 32% yield and that of 3,4-di-O-acetyl-2-azidodeoxy-α,β-L-fucose in 64% yield. Crystals of 3,4-di-O-acetyl-2-azidodeoxy-1-O-nitro-α-L-fucose suitable for X-ray analysis were obtained by vapor diffusion of hexane into a solution of the mixture of the nitrates in ethyl acetate.
Friedel pairs were merged prior to refinement and the absolute structure was assigned based on known stereocenters. All hydrogen atoms were placed in calculated positions and were isotropically refined using a riding model, with C—H = 0.97–1.00 Å and with Uiso(H) = 1.2 Ueq(C) or 1.5 Ueq(C) for methyl H atoms. Methyl hydrogen atoms were allowed to rotate to best fit the observed electron density. The e.s.d. values of the cell parameters are taken from the software recognizing that the values are unreasonably small (Herbstein, 2000).
Azidodeoxynitrates such as the tile compound serve as versatile intermediates in the preparation of a variety of aminosugar derivatives. 3,4-Di-O-acetyl-2-azidodeoxy-1-O-nitro-α-L-fucose (I) specifically was used as a precursor for the synthesis of L-fucosamine analogs, for example derivatives of the bacterial aminosugar N-acetyl-L-fucosamine (Anisuzzaman & Horton, 1987), as well as glycosyl phosphates through reaction with caesium dibenzyl phosphate (Illarionov et al., 1999).
(I) was prepared by the reaction of 3,4-di-O-acetyl-L-fucal (Figure 1, Hansen et al., 1999) with sodium azide and ceric ammonium nitrate in acetone leading to a mixture of 1-O-hydroxides and 1-O-nitrates with the 2-azidodeoxy-L-fucose configuration (Lehmann et al., 1979). The α and β 1-O-nitrates can be separated from the 1-O-hydroxides by column chromatography on silica gel, and from the mixture of the two nitrates the α-1-O-nitrate, namely 3,4-di-O-acetyl-2-azidodeoxy-1-O-nitro-α-L-fucose, was selectively crystallized by vapor diffusion of hexane into a solution of the nitrate mixture in ethyl acetate.
The title compound crystallizes in the orthorhombic chiral space group P212121 with two crystallographically independent molecules in the asymmetric part of the unit cell. The two independent molecules are chemically identical and both exhibit an almost perfect chair configuration (Figure 2). The rings adopt the 1C4 conformation with the azide groups at C2 in the expected equatorial and the nitrate at C1 in the axial position. The largest deviations between the two independent molecules is found for the azide and the two acetyl groups: the weighted r.m.s. deviation for an overlay of both molecules is 0.24 Å for all non-hydrogen atoms, but drops to only 0.08 Å when the N3 and OAc groups are omitted from the fit.
The observed arrangement seems to be stabilized by a range of C—H···O and also C—H···N hydrogen bonds. The most significant interactions with C···X distances below or close to the sum of the van der Waals radii (Vainshtein et al., 1982) are given in the hydrogen bonding table.
For related literature on the synthesis of the title compound, see: Hansen et al. (1999); Lehmann et al. (1979). For the use of the title compound in aminosugar synthesis, see: Anisuzzaman & Horton (1987); Illarionov et al. (1999).
For related literature, see: Herbstein (2000); Vainshtein et al. (1982).
Data collection: SMART (Bruker, 2002); cell refinement: SAINT-Plus (Bruker, 2003); data reduction: SAINT-Plus; program(s) used to solve structure: SHELXTL (Bruker, 2003); program(s) used to refine structure: SHELXTL; molecular graphics: SHELXTL; software used to prepare material for publication: SHELXTL.
C10H14N4O8 | F(000) = 1328 |
Mr = 318.25 | Dx = 1.488 Mg m−3 |
Orthorhombic, P212121 | Mo Kα radiation, λ = 0.71073 Å |
Hall symbol: P 2ac 2ab | Cell parameters from 8591 reflections |
a = 8.2169 (6) Å | θ = 2.3–30.5° |
b = 15.8825 (12) Å | µ = 0.13 mm−1 |
c = 21.7738 (16) Å | T = 100 K |
V = 2841.6 (4) Å3 | Block, colorless |
Z = 8 | 0.52 × 0.50 × 0.36 mm |
Bruker SMART APEX CCD diffractometer | 3960 independent reflections |
Radiation source: fine-focus sealed tube | 3777 reflections with I > 2σ(I) |
Graphite monochromator | Rint = 0.057 |
ω scans | θmax = 28.3°, θmin = 1.6° |
Absorption correction: multi-scan (SADABS in SAINT-Plus; Bruker, 2003) | h = −10→10 |
Tmin = 0.783, Tmax = 0.954 | k = −21→21 |
25826 measured reflections | l = −28→28 |
Refinement on F2 | Primary atom site location: structure-invariant direct methods |
Least-squares matrix: full | Secondary atom site location: difference Fourier map |
R[F2 > 2σ(F2)] = 0.033 | Hydrogen site location: inferred from neighbouring sites |
wR(F2) = 0.084 | H-atom parameters constrained |
S = 1.05 | w = 1/[σ2(Fo2) + (0.0432P)2 + 0.6271P] where P = (Fo2 + 2Fc2)/3 |
3960 reflections | (Δ/σ)max = 0.001 |
403 parameters | Δρmax = 0.27 e Å−3 |
0 restraints | Δρmin = −0.18 e Å−3 |
C10H14N4O8 | V = 2841.6 (4) Å3 |
Mr = 318.25 | Z = 8 |
Orthorhombic, P212121 | Mo Kα radiation |
a = 8.2169 (6) Å | µ = 0.13 mm−1 |
b = 15.8825 (12) Å | T = 100 K |
c = 21.7738 (16) Å | 0.52 × 0.50 × 0.36 mm |
Bruker SMART APEX CCD diffractometer | 3960 independent reflections |
Absorption correction: multi-scan (SADABS in SAINT-Plus; Bruker, 2003) | 3777 reflections with I > 2σ(I) |
Tmin = 0.783, Tmax = 0.954 | Rint = 0.057 |
25826 measured reflections |
R[F2 > 2σ(F2)] = 0.033 | 0 restraints |
wR(F2) = 0.084 | H-atom parameters constrained |
S = 1.05 | Δρmax = 0.27 e Å−3 |
3960 reflections | Δρmin = −0.18 e Å−3 |
403 parameters |
Geometry. All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell e.s.d.'s are taken into account individually in the estimation of e.s.d.'s in distances, angles and torsion angles; correlations between e.s.d.'s in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell e.s.d.'s is used for estimating e.s.d.'s involving l.s. planes. |
Refinement. Refinement of F2 against ALL reflections. The weighted R-factor wR and goodness of fit S are based on F2, conventional R-factors R are based on F, with F set to zero for negative F2. The threshold expression of F2 > σ(F2) is used only for calculating R-factors(gt) etc. and is not relevant to the choice of reflections for refinement. R-factors based on F2 are statistically about twice as large as those based on F, and R- factors based on ALL data will be even larger. |
x | y | z | Uiso*/Ueq | ||
C1A | 0.1519 (2) | 0.68931 (11) | 0.96074 (8) | 0.0173 (3) | |
H1A | 0.1697 | 0.6907 | 1.0062 | 0.021* | |
C2A | 0.3138 (2) | 0.70045 (11) | 0.92821 (8) | 0.0165 (3) | |
H2A | 0.3653 | 0.7545 | 0.9416 | 0.020* | |
C3A | 0.2871 (2) | 0.70192 (11) | 0.85901 (8) | 0.0160 (3) | |
H3A | 0.2445 | 0.6460 | 0.8452 | 0.019* | |
C4A | 0.1645 (2) | 0.77066 (11) | 0.84250 (8) | 0.0171 (3) | |
H4A | 0.1394 | 0.7679 | 0.7976 | 0.021* | |
C5A | 0.0083 (2) | 0.75793 (11) | 0.87924 (8) | 0.0178 (3) | |
H5A | −0.0439 | 0.7043 | 0.8654 | 0.021* | |
C6A | −0.1136 (2) | 0.82860 (12) | 0.87166 (9) | 0.0234 (4) | |
H6A1 | −0.0730 | 0.8792 | 0.8924 | 0.035* | |
H6A2 | −0.2178 | 0.8119 | 0.8898 | 0.035* | |
H6A3 | −0.1288 | 0.8405 | 0.8279 | 0.035* | |
C7A | 0.4653 (2) | 0.68822 (11) | 0.77365 (8) | 0.0180 (3) | |
C8A | 0.6392 (2) | 0.69823 (12) | 0.75485 (9) | 0.0233 (4) | |
H8A1 | 0.6533 | 0.6775 | 0.7128 | 0.035* | |
H8A2 | 0.7089 | 0.6659 | 0.7827 | 0.035* | |
H8A3 | 0.6694 | 0.7579 | 0.7567 | 0.035* | |
C9A | 0.3093 (3) | 0.89460 (12) | 0.81195 (9) | 0.0230 (4) | |
C10A | 0.3549 (3) | 0.98127 (13) | 0.83182 (10) | 0.0301 (5) | |
H10A | 0.2568 | 1.0161 | 0.8347 | 0.045* | |
H10B | 0.4297 | 1.0060 | 0.8018 | 0.045* | |
H10C | 0.4082 | 0.9787 | 0.8720 | 0.045* | |
C1B | 0.2547 (2) | 0.43484 (11) | 0.79007 (8) | 0.0180 (3) | |
H1B | 0.3018 | 0.4367 | 0.7477 | 0.022* | |
C2B | 0.3935 (2) | 0.43329 (11) | 0.83734 (8) | 0.0178 (3) | |
H2B | 0.4627 | 0.3826 | 0.8297 | 0.021* | |
C3B | 0.3204 (2) | 0.42703 (11) | 0.90164 (8) | 0.0163 (3) | |
H3B | 0.2521 | 0.4777 | 0.9105 | 0.020* | |
C4B | 0.2164 (2) | 0.34711 (11) | 0.90480 (8) | 0.0175 (3) | |
H4B | 0.1664 | 0.3415 | 0.9465 | 0.021* | |
C5B | 0.0837 (2) | 0.35172 (11) | 0.85600 (8) | 0.0178 (3) | |
H5B | 0.0102 | 0.4000 | 0.8660 | 0.021* | |
C6B | −0.0187 (3) | 0.27262 (13) | 0.85109 (9) | 0.0251 (4) | |
H6B1 | 0.0490 | 0.2262 | 0.8361 | 0.038* | |
H6B2 | −0.1086 | 0.2823 | 0.8224 | 0.038* | |
H6B3 | −0.0624 | 0.2582 | 0.8916 | 0.038* | |
C7B | 0.4189 (2) | 0.44343 (11) | 1.00306 (8) | 0.0198 (4) | |
C8B | 0.5676 (3) | 0.43756 (15) | 1.04241 (10) | 0.0304 (4) | |
H8B1 | 0.5360 | 0.4401 | 1.0858 | 0.046* | |
H8B2 | 0.6407 | 0.4845 | 1.0329 | 0.046* | |
H8B3 | 0.6234 | 0.3842 | 1.0343 | 0.046* | |
C9B | 0.3866 (2) | 0.23554 (11) | 0.94182 (9) | 0.0204 (4) | |
C10B | 0.5124 (3) | 0.17257 (13) | 0.92196 (10) | 0.0274 (4) | |
H10D | 0.4929 | 0.1189 | 0.9428 | 0.041* | |
H10E | 0.6210 | 0.1934 | 0.9326 | 0.041* | |
H10F | 0.5054 | 0.1644 | 0.8774 | 0.041* | |
N1A | −0.0035 (2) | 0.56522 (10) | 0.98752 (7) | 0.0216 (3) | |
N2A | 0.41780 (19) | 0.62882 (10) | 0.94692 (7) | 0.0184 (3) | |
N3A | 0.5655 (2) | 0.64117 (10) | 0.94132 (7) | 0.0206 (3) | |
N4A | 0.7021 (2) | 0.64433 (12) | 0.93857 (10) | 0.0313 (4) | |
N1B | 0.0763 (2) | 0.54341 (10) | 0.75172 (7) | 0.0226 (3) | |
N2B | 0.4937 (2) | 0.50950 (10) | 0.82922 (8) | 0.0209 (3) | |
N3B | 0.6420 (2) | 0.49697 (10) | 0.83443 (8) | 0.0230 (3) | |
N4B | 0.7785 (2) | 0.49429 (13) | 0.83732 (11) | 0.0374 (5) | |
O1A | 0.04098 (16) | 0.75143 (8) | 0.94464 (6) | 0.0181 (3) | |
O2A | 0.09418 (16) | 0.60611 (8) | 0.94303 (6) | 0.0188 (3) | |
O3A | 0.44471 (16) | 0.71616 (8) | 0.83227 (6) | 0.0181 (3) | |
O4A | 0.22958 (17) | 0.85305 (8) | 0.85736 (6) | 0.0191 (3) | |
O5A | −0.0240 (2) | 0.59973 (10) | 1.03602 (7) | 0.0311 (3) | |
O6A | −0.05346 (18) | 0.49872 (9) | 0.96954 (7) | 0.0280 (3) | |
O7A | 0.35749 (18) | 0.65942 (9) | 0.74306 (6) | 0.0237 (3) | |
O8A | 0.3355 (2) | 0.86470 (9) | 0.76178 (7) | 0.0311 (3) | |
O1B | 0.15313 (16) | 0.36579 (8) | 0.79517 (6) | 0.0189 (3) | |
O2B | 0.16796 (16) | 0.51307 (8) | 0.80211 (6) | 0.0194 (3) | |
O3B | 0.45529 (16) | 0.42301 (8) | 0.94370 (6) | 0.0191 (3) | |
O4B | 0.32046 (16) | 0.27581 (8) | 0.89237 (6) | 0.0180 (3) | |
O5B | 0.0821 (2) | 0.50576 (10) | 0.70412 (7) | 0.0371 (4) | |
O6B | 0.00131 (18) | 0.60629 (9) | 0.76422 (7) | 0.0265 (3) | |
O7B | 0.28545 (17) | 0.46294 (9) | 1.01992 (6) | 0.0228 (3) | |
O8B | 0.3492 (2) | 0.25016 (10) | 0.99387 (7) | 0.0291 (3) |
U11 | U22 | U33 | U12 | U13 | U23 | |
C1A | 0.0205 (8) | 0.0149 (7) | 0.0164 (7) | 0.0001 (7) | 0.0012 (7) | 0.0008 (6) |
C2A | 0.0171 (8) | 0.0150 (7) | 0.0172 (8) | −0.0007 (6) | 0.0006 (6) | 0.0001 (6) |
C3A | 0.0177 (8) | 0.0159 (7) | 0.0144 (7) | −0.0017 (7) | 0.0033 (6) | −0.0001 (6) |
C4A | 0.0194 (8) | 0.0172 (8) | 0.0148 (8) | −0.0010 (7) | 0.0007 (6) | 0.0003 (6) |
C5A | 0.0184 (8) | 0.0185 (8) | 0.0164 (8) | 0.0013 (7) | 0.0003 (6) | 0.0011 (7) |
C6A | 0.0231 (9) | 0.0234 (9) | 0.0237 (9) | 0.0048 (8) | 0.0011 (7) | 0.0028 (7) |
C7A | 0.0216 (9) | 0.0152 (7) | 0.0172 (8) | −0.0002 (7) | 0.0021 (6) | 0.0022 (6) |
C8A | 0.0211 (9) | 0.0256 (9) | 0.0231 (9) | −0.0024 (8) | 0.0039 (7) | 0.0001 (7) |
C9A | 0.0281 (9) | 0.0195 (8) | 0.0215 (9) | −0.0014 (8) | 0.0020 (7) | 0.0043 (7) |
C10A | 0.0432 (12) | 0.0219 (9) | 0.0252 (10) | −0.0069 (9) | 0.0032 (9) | 0.0023 (8) |
C1B | 0.0206 (8) | 0.0161 (8) | 0.0175 (8) | 0.0020 (7) | −0.0007 (7) | 0.0003 (6) |
C2B | 0.0189 (8) | 0.0148 (7) | 0.0198 (8) | 0.0001 (7) | −0.0006 (7) | 0.0010 (7) |
C3B | 0.0158 (8) | 0.0161 (7) | 0.0169 (8) | 0.0012 (7) | −0.0018 (6) | 0.0003 (6) |
C4B | 0.0182 (8) | 0.0178 (8) | 0.0164 (8) | 0.0011 (7) | −0.0005 (6) | −0.0015 (6) |
C5B | 0.0176 (8) | 0.0202 (8) | 0.0156 (8) | −0.0003 (7) | 0.0000 (6) | −0.0003 (7) |
C6B | 0.0257 (10) | 0.0261 (9) | 0.0235 (9) | −0.0068 (8) | −0.0011 (8) | 0.0011 (8) |
C7B | 0.0236 (9) | 0.0171 (8) | 0.0187 (8) | −0.0035 (7) | −0.0029 (7) | 0.0013 (7) |
C8B | 0.0263 (10) | 0.0427 (12) | 0.0221 (9) | −0.0012 (9) | −0.0072 (8) | −0.0013 (9) |
C9B | 0.0224 (8) | 0.0167 (8) | 0.0220 (9) | −0.0022 (7) | −0.0041 (7) | 0.0030 (7) |
C10B | 0.0314 (10) | 0.0229 (9) | 0.0278 (10) | 0.0079 (8) | −0.0064 (8) | 0.0008 (8) |
N1A | 0.0185 (7) | 0.0208 (7) | 0.0256 (8) | 0.0022 (6) | 0.0053 (6) | 0.0076 (7) |
N2A | 0.0169 (7) | 0.0165 (7) | 0.0218 (7) | −0.0007 (6) | −0.0002 (6) | 0.0025 (6) |
N3A | 0.0225 (8) | 0.0175 (7) | 0.0218 (7) | 0.0019 (6) | 0.0006 (6) | 0.0008 (6) |
N4A | 0.0206 (8) | 0.0277 (9) | 0.0457 (11) | 0.0021 (7) | 0.0019 (8) | 0.0040 (8) |
N1B | 0.0247 (8) | 0.0206 (7) | 0.0225 (8) | 0.0005 (7) | −0.0058 (6) | 0.0022 (6) |
N2B | 0.0186 (7) | 0.0180 (7) | 0.0262 (8) | 0.0002 (6) | 0.0008 (6) | 0.0016 (6) |
N3B | 0.0228 (8) | 0.0204 (7) | 0.0257 (8) | −0.0015 (7) | −0.0011 (6) | 0.0011 (6) |
N4B | 0.0219 (9) | 0.0304 (9) | 0.0600 (14) | −0.0021 (8) | −0.0063 (9) | 0.0018 (9) |
O1A | 0.0210 (6) | 0.0177 (6) | 0.0157 (6) | 0.0033 (5) | 0.0010 (5) | −0.0003 (5) |
O2A | 0.0203 (6) | 0.0165 (6) | 0.0196 (6) | −0.0020 (5) | 0.0047 (5) | 0.0015 (5) |
O3A | 0.0174 (6) | 0.0191 (6) | 0.0178 (6) | −0.0034 (5) | 0.0028 (5) | 0.0000 (5) |
O4A | 0.0242 (6) | 0.0158 (6) | 0.0173 (6) | −0.0021 (5) | 0.0019 (5) | 0.0013 (5) |
O5A | 0.0384 (9) | 0.0290 (7) | 0.0259 (7) | 0.0035 (7) | 0.0150 (6) | 0.0057 (6) |
O6A | 0.0230 (7) | 0.0210 (7) | 0.0400 (8) | −0.0047 (6) | 0.0017 (6) | 0.0063 (6) |
O7A | 0.0246 (7) | 0.0259 (7) | 0.0205 (6) | −0.0027 (6) | 0.0020 (5) | −0.0024 (5) |
O8A | 0.0464 (9) | 0.0244 (7) | 0.0225 (7) | −0.0026 (7) | 0.0091 (7) | 0.0026 (6) |
O1B | 0.0229 (6) | 0.0174 (6) | 0.0162 (6) | −0.0012 (5) | 0.0006 (5) | −0.0002 (5) |
O2B | 0.0234 (6) | 0.0170 (6) | 0.0179 (6) | 0.0033 (5) | −0.0033 (5) | 0.0010 (5) |
O3B | 0.0183 (6) | 0.0208 (6) | 0.0181 (6) | 0.0008 (5) | −0.0033 (5) | −0.0006 (5) |
O4B | 0.0208 (6) | 0.0152 (5) | 0.0179 (6) | 0.0020 (5) | −0.0022 (5) | 0.0003 (5) |
O5B | 0.0551 (10) | 0.0321 (8) | 0.0242 (7) | 0.0103 (8) | −0.0154 (7) | −0.0057 (6) |
O6B | 0.0235 (6) | 0.0224 (6) | 0.0337 (8) | 0.0047 (6) | −0.0022 (6) | 0.0023 (6) |
O7B | 0.0240 (7) | 0.0242 (7) | 0.0201 (6) | −0.0010 (6) | 0.0010 (5) | −0.0004 (5) |
O8B | 0.0382 (8) | 0.0292 (7) | 0.0198 (7) | 0.0051 (7) | −0.0022 (6) | 0.0040 (5) |
C1A—O1A | 1.388 (2) | C2B—C3B | 1.527 (2) |
C1A—O2A | 1.456 (2) | C2B—H2B | 1.0000 |
C1A—C2A | 1.518 (2) | C3B—O3B | 1.440 (2) |
C1A—H1A | 1.0000 | C3B—C4B | 1.532 (2) |
C2A—N2A | 1.480 (2) | C3B—H3B | 1.0000 |
C2A—C3A | 1.523 (2) | C4B—O4B | 1.445 (2) |
C2A—H2A | 1.0000 | C4B—C5B | 1.524 (2) |
C3A—O3A | 1.438 (2) | C4B—H4B | 1.0000 |
C3A—C4A | 1.529 (2) | C5B—O1B | 1.459 (2) |
C3A—H3A | 1.0000 | C5B—C6B | 1.516 (3) |
C4A—O4A | 1.450 (2) | C5B—H5B | 1.0000 |
C4A—C5A | 1.526 (2) | C6B—H6B1 | 0.9800 |
C4A—H4A | 1.0000 | C6B—H6B2 | 0.9800 |
C5A—O1A | 1.453 (2) | C6B—H6B3 | 0.9800 |
C5A—C6A | 1.513 (3) | C7B—O7B | 1.197 (2) |
C5A—H5A | 1.0000 | C7B—O3B | 1.366 (2) |
C6A—H6A1 | 0.9800 | C7B—C8B | 1.495 (3) |
C6A—H6A2 | 0.9800 | C8B—H8B1 | 0.9800 |
C6A—H6A3 | 0.9800 | C8B—H8B2 | 0.9800 |
C7A—O7A | 1.199 (2) | C8B—H8B3 | 0.9800 |
C7A—O3A | 1.362 (2) | C9B—O8B | 1.197 (2) |
C7A—C8A | 1.495 (3) | C9B—O4B | 1.365 (2) |
C8A—H8A1 | 0.9800 | C9B—C10B | 1.502 (3) |
C8A—H8A2 | 0.9800 | C10B—H10D | 0.9800 |
C8A—H8A3 | 0.9800 | C10B—H10E | 0.9800 |
C9A—O8A | 1.210 (2) | C10B—H10F | 0.9800 |
C9A—O4A | 1.357 (2) | N1A—O6A | 1.199 (2) |
C9A—C10A | 1.491 (3) | N1A—O5A | 1.202 (2) |
C10A—H10A | 0.9800 | N1A—O2A | 1.4158 (19) |
C10A—H10B | 0.9800 | N2A—N3A | 1.236 (2) |
C10A—H10C | 0.9800 | N3A—N4A | 1.125 (2) |
C1B—O1B | 1.383 (2) | N1B—O5B | 1.198 (2) |
C1B—O2B | 1.456 (2) | N1B—O6B | 1.204 (2) |
C1B—C2B | 1.536 (2) | N1B—O2B | 1.4156 (19) |
C1B—H1B | 1.0000 | N2B—N3B | 1.240 (2) |
C2B—N2B | 1.474 (2) | N3B—N4B | 1.124 (3) |
O1A—C1A—O2A | 111.38 (14) | N2B—C2B—H2B | 108.9 |
O1A—C1A—C2A | 112.01 (14) | C3B—C2B—H2B | 108.9 |
O2A—C1A—C2A | 105.52 (14) | C1B—C2B—H2B | 108.9 |
O1A—C1A—H1A | 109.3 | O3B—C3B—C2B | 106.45 (14) |
O2A—C1A—H1A | 109.3 | O3B—C3B—C4B | 111.37 (14) |
C2A—C1A—H1A | 109.3 | C2B—C3B—C4B | 108.34 (14) |
N2A—C2A—C1A | 106.74 (14) | O3B—C3B—H3B | 110.2 |
N2A—C2A—C3A | 111.55 (14) | C2B—C3B—H3B | 110.2 |
C1A—C2A—C3A | 109.69 (15) | C4B—C3B—H3B | 110.2 |
N2A—C2A—H2A | 109.6 | O4B—C4B—C5B | 109.31 (14) |
C1A—C2A—H2A | 109.6 | O4B—C4B—C3B | 108.11 (14) |
C3A—C2A—H2A | 109.6 | C5B—C4B—C3B | 109.14 (14) |
O3A—C3A—C2A | 105.84 (14) | O4B—C4B—H4B | 110.1 |
O3A—C3A—C4A | 112.72 (13) | C5B—C4B—H4B | 110.1 |
C2A—C3A—C4A | 109.80 (14) | C3B—C4B—H4B | 110.1 |
O3A—C3A—H3A | 109.5 | O1B—C5B—C6B | 106.25 (14) |
C2A—C3A—H3A | 109.5 | O1B—C5B—C4B | 111.12 (14) |
C4A—C3A—H3A | 109.5 | C6B—C5B—C4B | 113.97 (15) |
O4A—C4A—C5A | 108.24 (14) | O1B—C5B—H5B | 108.5 |
O4A—C4A—C3A | 110.41 (14) | C6B—C5B—H5B | 108.5 |
C5A—C4A—C3A | 109.66 (14) | C4B—C5B—H5B | 108.5 |
O4A—C4A—H4A | 109.5 | C5B—C6B—H6B1 | 109.5 |
C5A—C4A—H4A | 109.5 | C5B—C6B—H6B2 | 109.5 |
C3A—C4A—H4A | 109.5 | H6B1—C6B—H6B2 | 109.5 |
O1A—C5A—C6A | 106.39 (14) | C5B—C6B—H6B3 | 109.5 |
O1A—C5A—C4A | 111.57 (14) | H6B1—C6B—H6B3 | 109.5 |
C6A—C5A—C4A | 113.64 (15) | H6B2—C6B—H6B3 | 109.5 |
O1A—C5A—H5A | 108.4 | O7B—C7B—O3B | 123.52 (17) |
C6A—C5A—H5A | 108.4 | O7B—C7B—C8B | 126.08 (18) |
C4A—C5A—H5A | 108.4 | O3B—C7B—C8B | 110.40 (16) |
C5A—C6A—H6A1 | 109.5 | C7B—C8B—H8B1 | 109.5 |
C5A—C6A—H6A2 | 109.5 | C7B—C8B—H8B2 | 109.5 |
H6A1—C6A—H6A2 | 109.5 | H8B1—C8B—H8B2 | 109.5 |
C5A—C6A—H6A3 | 109.5 | C7B—C8B—H8B3 | 109.5 |
H6A1—C6A—H6A3 | 109.5 | H8B1—C8B—H8B3 | 109.5 |
H6A2—C6A—H6A3 | 109.5 | H8B2—C8B—H8B3 | 109.5 |
O7A—C7A—O3A | 123.58 (17) | O8B—C9B—O4B | 123.61 (18) |
O7A—C7A—C8A | 126.49 (17) | O8B—C9B—C10B | 125.35 (18) |
O3A—C7A—C8A | 109.92 (16) | O4B—C9B—C10B | 111.03 (16) |
C7A—C8A—H8A1 | 109.5 | C9B—C10B—H10D | 109.5 |
C7A—C8A—H8A2 | 109.5 | C9B—C10B—H10E | 109.5 |
H8A1—C8A—H8A2 | 109.5 | H10D—C10B—H10E | 109.5 |
C7A—C8A—H8A3 | 109.5 | C9B—C10B—H10F | 109.5 |
H8A1—C8A—H8A3 | 109.5 | H10D—C10B—H10F | 109.5 |
H8A2—C8A—H8A3 | 109.5 | H10E—C10B—H10F | 109.5 |
O8A—C9A—O4A | 123.53 (18) | O6A—N1A—O5A | 129.84 (17) |
O8A—C9A—C10A | 125.42 (18) | O6A—N1A—O2A | 112.01 (15) |
O4A—C9A—C10A | 111.03 (16) | O5A—N1A—O2A | 118.14 (16) |
C9A—C10A—H10A | 109.5 | N3A—N2A—C2A | 114.70 (15) |
C9A—C10A—H10B | 109.5 | N4A—N3A—N2A | 172.9 (2) |
H10A—C10A—H10B | 109.5 | O5B—N1B—O6B | 129.07 (17) |
C9A—C10A—H10C | 109.5 | O5B—N1B—O2B | 118.64 (15) |
H10A—C10A—H10C | 109.5 | O6B—N1B—O2B | 112.29 (15) |
H10B—C10A—H10C | 109.5 | N3B—N2B—C2B | 113.95 (16) |
O1B—C1B—O2B | 111.51 (14) | N4B—N3B—N2B | 172.6 (2) |
O1B—C1B—C2B | 112.43 (14) | C1A—O1A—C5A | 114.81 (13) |
O2B—C1B—C2B | 104.85 (14) | N1A—O2A—C1A | 114.81 (13) |
O1B—C1B—H1B | 109.3 | C7A—O3A—C3A | 116.11 (14) |
O2B—C1B—H1B | 109.3 | C9A—O4A—C4A | 117.02 (14) |
C2B—C1B—H1B | 109.3 | C1B—O1B—C5B | 115.50 (13) |
N2B—C2B—C3B | 112.53 (15) | N1B—O2B—C1B | 114.29 (13) |
N2B—C2B—C1B | 108.72 (14) | C7B—O3B—C3B | 115.02 (14) |
C3B—C2B—C1B | 108.86 (15) | C9B—O4B—C4B | 117.08 (14) |
O1A—C1A—C2A—N2A | −177.50 (14) | C3B—C2B—N2B—N3B | 99.33 (19) |
O2A—C1A—C2A—N2A | −56.15 (17) | C1B—C2B—N2B—N3B | −140.00 (17) |
O1A—C1A—C2A—C3A | −56.49 (19) | O2A—C1A—O1A—C5A | −61.11 (19) |
O2A—C1A—C2A—C3A | 64.86 (16) | C2A—C1A—O1A—C5A | 56.80 (19) |
N2A—C2A—C3A—O3A | −64.30 (17) | C6A—C5A—O1A—C1A | −179.81 (14) |
C1A—C2A—C3A—O3A | 177.64 (13) | C4A—C5A—O1A—C1A | −55.36 (19) |
N2A—C2A—C3A—C4A | 173.77 (14) | O6A—N1A—O2A—C1A | 176.94 (15) |
C1A—C2A—C3A—C4A | 55.71 (18) | O5A—N1A—O2A—C1A | −3.3 (2) |
O3A—C3A—C4A—O4A | −53.14 (18) | O1A—C1A—O2A—N1A | −88.22 (17) |
C2A—C3A—C4A—O4A | 64.59 (18) | C2A—C1A—O2A—N1A | 150.01 (14) |
O3A—C3A—C4A—C5A | −172.32 (14) | O7A—C7A—O3A—C3A | 5.9 (2) |
C2A—C3A—C4A—C5A | −54.59 (19) | C8A—C7A—O3A—C3A | −173.41 (14) |
O4A—C4A—C5A—O1A | −67.51 (17) | C2A—C3A—O3A—C7A | 155.22 (14) |
C3A—C4A—C5A—O1A | 53.00 (19) | C4A—C3A—O3A—C7A | −84.75 (18) |
O4A—C4A—C5A—C6A | 52.77 (19) | O8A—C9A—O4A—C4A | −4.0 (3) |
C3A—C4A—C5A—C6A | 173.28 (15) | C10A—C9A—O4A—C4A | 174.42 (16) |
O1B—C1B—C2B—N2B | −179.13 (14) | C5A—C4A—O4A—C9A | −147.08 (16) |
O2B—C1B—C2B—N2B | −57.80 (17) | C3A—C4A—O4A—C9A | 92.88 (18) |
O1B—C1B—C2B—C3B | −56.22 (19) | O2B—C1B—O1B—C5B | −62.93 (18) |
O2B—C1B—C2B—C3B | 65.10 (17) | C2B—C1B—O1B—C5B | 54.51 (19) |
N2B—C2B—C3B—O3B | −61.27 (18) | C6B—C5B—O1B—C1B | −178.66 (15) |
C1B—C2B—C3B—O3B | 178.14 (13) | C4B—C5B—O1B—C1B | −54.18 (19) |
N2B—C2B—C3B—C4B | 178.85 (14) | O5B—N1B—O2B—C1B | −2.9 (2) |
C1B—C2B—C3B—C4B | 58.26 (18) | O6B—N1B—O2B—C1B | 177.26 (15) |
O3B—C3B—C4B—O4B | −56.78 (17) | O1B—C1B—O2B—N1B | −80.51 (17) |
C2B—C3B—C4B—O4B | 59.97 (17) | C2B—C1B—O2B—N1B | 157.57 (14) |
O3B—C3B—C4B—C5B | −175.58 (14) | O7B—C7B—O3B—C3B | 0.4 (3) |
C2B—C3B—C4B—C5B | −58.83 (18) | C8B—C7B—O3B—C3B | −179.70 (15) |
O4B—C4B—C5B—O1B | −62.93 (18) | C2B—C3B—O3B—C7B | 160.42 (14) |
C3B—C4B—C5B—O1B | 55.12 (18) | C4B—C3B—O3B—C7B | −81.68 (18) |
O4B—C4B—C5B—C6B | 57.07 (19) | O8B—C9B—O4B—C4B | 8.5 (3) |
C3B—C4B—C5B—C6B | 175.12 (15) | C10B—C9B—O4B—C4B | −170.63 (15) |
C1A—C2A—N2A—N3A | −157.79 (16) | C5B—C4B—O4B—C9B | −147.23 (15) |
C3A—C2A—N2A—N3A | 82.4 (2) | C3B—C4B—O4B—C9B | 94.07 (17) |
D—H···A | D—H | H···A | D···A | D—H···A |
C10A—H10C···O5Ai | 0.98 | 2.42 | 3.305 (3) | 150 |
C8A—H8A3···O1Bii | 0.98 | 2.52 | 3.344 (2) | 142 |
C5B—H5B···N4Biii | 1.00 | 2.50 | 3.403 (3) | 150 |
C4B—H4B···O8B | 1.00 | 2.33 | 2.706 (2) | 101 |
C3B—H3B···O2A | 1.00 | 2.52 | 3.515 (2) | 174 |
C3A—H3A···O2B | 1.00 | 2.40 | 3.390 (2) | 173 |
C2B—H2B···O4B | 1.00 | 2.47 | 2.838 (2) | 101 |
C2A—H2A···O5Ai | 1.00 | 2.54 | 3.529 (2) | 173 |
Symmetry codes: (i) x+1/2, −y+3/2, −z+2; (ii) −x+1, y+1/2, −z+3/2; (iii) x−1, y, z. |
Experimental details
Crystal data | |
Chemical formula | C10H14N4O8 |
Mr | 318.25 |
Crystal system, space group | Orthorhombic, P212121 |
Temperature (K) | 100 |
a, b, c (Å) | 8.2169 (6), 15.8825 (12), 21.7738 (16) |
V (Å3) | 2841.6 (4) |
Z | 8 |
Radiation type | Mo Kα |
µ (mm−1) | 0.13 |
Crystal size (mm) | 0.52 × 0.50 × 0.36 |
Data collection | |
Diffractometer | Bruker SMART APEX CCD diffractometer |
Absorption correction | Multi-scan (SADABS in SAINT-Plus; Bruker, 2003) |
Tmin, Tmax | 0.783, 0.954 |
No. of measured, independent and observed [I > 2σ(I)] reflections | 25826, 3960, 3777 |
Rint | 0.057 |
(sin θ/λ)max (Å−1) | 0.667 |
Refinement | |
R[F2 > 2σ(F2)], wR(F2), S | 0.033, 0.084, 1.05 |
No. of reflections | 3960 |
No. of parameters | 403 |
H-atom treatment | H-atom parameters constrained |
Δρmax, Δρmin (e Å−3) | 0.27, −0.18 |
Computer programs: SMART (Bruker, 2002), SAINT-Plus (Bruker, 2003), SAINT-Plus, SHELXTL (Bruker, 2003), SHELXTL.
D—H···A | D—H | H···A | D···A | D—H···A |
C10A—H10C···O5Ai | 0.98 | 2.42 | 3.305 (3) | 149.6 |
C8A—H8A3···O1Bii | 0.98 | 2.52 | 3.344 (2) | 141.8 |
C5B—H5B···N4Biii | 1.00 | 2.50 | 3.403 (3) | 149.8 |
C4B—H4B···O8B | 1.00 | 2.33 | 2.706 (2) | 101.1 |
C3B—H3B···O2A | 1.00 | 2.52 | 3.515 (2) | 174.3 |
C3A—H3A···O2B | 1.00 | 2.40 | 3.390 (2) | 172.7 |
C2B—H2B···O4B | 1.00 | 2.47 | 2.838 (2) | 101.1 |
C2A—H2A···O5Ai | 1.00 | 2.54 | 3.529 (2) | 172.6 |
Symmetry codes: (i) x+1/2, −y+3/2, −z+2; (ii) −x+1, y+1/2, −z+3/2; (iii) x−1, y, z. |
Azidodeoxynitrates such as the tile compound serve as versatile intermediates in the preparation of a variety of aminosugar derivatives. 3,4-Di-O-acetyl-2-azidodeoxy-1-O-nitro-α-L-fucose (I) specifically was used as a precursor for the synthesis of L-fucosamine analogs, for example derivatives of the bacterial aminosugar N-acetyl-L-fucosamine (Anisuzzaman & Horton, 1987), as well as glycosyl phosphates through reaction with caesium dibenzyl phosphate (Illarionov et al., 1999).
(I) was prepared by the reaction of 3,4-di-O-acetyl-L-fucal (Figure 1, Hansen et al., 1999) with sodium azide and ceric ammonium nitrate in acetone leading to a mixture of 1-O-hydroxides and 1-O-nitrates with the 2-azidodeoxy-L-fucose configuration (Lehmann et al., 1979). The α and β 1-O-nitrates can be separated from the 1-O-hydroxides by column chromatography on silica gel, and from the mixture of the two nitrates the α-1-O-nitrate, namely 3,4-di-O-acetyl-2-azidodeoxy-1-O-nitro-α-L-fucose, was selectively crystallized by vapor diffusion of hexane into a solution of the nitrate mixture in ethyl acetate.
The title compound crystallizes in the orthorhombic chiral space group P212121 with two crystallographically independent molecules in the asymmetric part of the unit cell. The two independent molecules are chemically identical and both exhibit an almost perfect chair configuration (Figure 2). The rings adopt the 1C4 conformation with the azide groups at C2 in the expected equatorial and the nitrate at C1 in the axial position. The largest deviations between the two independent molecules is found for the azide and the two acetyl groups: the weighted r.m.s. deviation for an overlay of both molecules is 0.24 Å for all non-hydrogen atoms, but drops to only 0.08 Å when the N3 and OAc groups are omitted from the fit.
The observed arrangement seems to be stabilized by a range of C—H···O and also C—H···N hydrogen bonds. The most significant interactions with C···X distances below or close to the sum of the van der Waals radii (Vainshtein et al., 1982) are given in the hydrogen bonding table.