Structural Biology and Crystallization Communications

 

Acta Cryst. (2005). F61, 541-545  [ doi:10.1107/S1744309105013813 ]

High-resolution structure of the native histone octamer

C. M. Wood, J. M. Nicholson, S. J. Lambert, L. Chantalat, C. D. Reynolds and J. P. Baldwin

Synopsis: The high-resolution (1.90 Å) model of the native histone octamer allows structural comparisons to be made with the nucleosome-core particle, along with an identification of a likely core-histone binding site.

PDB reference: 1tzy

Online 1 June 2005

 

Acta Cryst. (2005). F61, 546-549  [ doi:10.1107/S1744309105015447 ]

Structure of Citrobacter freundii L-methionine -lyase

D. V. Mamaeva, E. A. Morozova, A. D. Nikulin, S. V. Revtovich, S. V. Nikonov, M. B. Garber and T. V. Demidkina

Synopsis: The three-dimensional structure of C. freundii L-methionine -lyase has been determined at 1.9 Å resolution.

PDB reference: 1y4i

Online 1 June 2005

 

Acta Cryst. (2005). F61, 550-552  [ doi:10.1107/S1744309105013278 ]

Purification, crystallization and preliminary diffraction studies of an ectromelia virus glutaredoxin

J.-P. Bacik, A. M. Brigley, L. D. Channon, G. F. Audette and B. Hazes

Synopsis: Ectromelia virus glutaredoxin has been crystallized in the presence of the reducing agent DTT. A diffraction data set has been collected and processed to 1.8 Å resolution.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 553-557  [ doi:10.1107/S1744309105013473 ]

Overexpression, purification and crystallographic analysis of a unique adenosine kinase from Mycobacterium tuberculosis

Y. Wang, M. C. Long, S. Ranganathan, V. Escuyer, W. B. Parker and R. Li

Synopsis: Adenosine kinase from M. tuberculosis has been overexpressed, purified and crystallized in the presence of adenosine. Structure determination using molecular replacement with diffraction data collected at 2.2 Å reveals a dimeric structure.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 558-561  [ doi:10.1107/S1744309105014132 ]

Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8

M. D. Lane, H.-J. Nam, E. Padron, B. Gurda-Whitaker, E. Kohlbrenner, G. Aslanidi, B. Byrne, R. McKenna, N. Muzyczka, S. Zolotukhin and M. Agbandje-McKenna

Synopsis: The production, purification, crystallization and preliminary X-ray crystallographic analysis of adeno-associated virus serotype 8 is reported.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 562-564  [ doi:10.1107/S174430910501657X ]

Crystallization and preliminary X-ray diffraction studies of the cysteine protease ervatamin A from Ervatamia coronaria

S. Chakraborty, S. Biswas, C. Chakrabarti and J. K. Dattagupta

Synopsis: Ervatamin A is a papain-family cysteine protease with high activity and stability. It has been isolated and purified from the latex of the medicinal flowering plant E. coronaria and crystallized by the vapour-diffusion technique. Crystals diffracted to 2.1 Å and the structure was solved by molecular replacement.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 565-568  [ doi:10.1107/S1744309105014235 ]

Crystallization of a protein using dehydration without a precipitant

M. L. Sharpe, E. N. Baker and J. S. Lott

Synopsis: Crystals grown by simple dehydration from a solution of hypoxic response protein I (HRPI) from M. tuberculosis diffract to a maximum resolution of 2.1 Å

Online 1 June 2005

 

Acta Cryst. (2005). F61, 569-572  [ doi:10.1107/S1744309105013904 ]

Acanthamoeba polyphaga mimivirus NDK: preliminary crystallographic analysis of the first viral nucleoside diphosphate kinase

S. Jeudy, B. Coutard, R. Lebrun and C. Abergel

Synopsis: A. polyphaga mimivirus, the largest known double-stranded DNA virus, is the first virus to exhibit a nucleoside diphosphate kinase gene. The expression and crystallization of the viral NDK are reported.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 573-576  [ doi:10.1107/S1744309105014363 ]

Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA

J. W. Crotty, C. Etzkorn, C. F. Barbas, D. J. Segal and N. C. Horton

Synopsis: Preliminary data analysis of crystals of a designed six-finger zinc-finger protein bound to DNA is presented. The cell likely contains two protein-DNA complexes and diffracts to 2.95 Å.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 577-580  [ doi:10.1107/S1744309105014557 ]

Crystallization and preliminary X-ray diffraction analysis of the electron-transfer complex between the terminal oxygenase component and ferredoxin in the Rieske non-haem iron oxygenase system carbazole 1,9a-dioxygenase

Y. Ashikawa, Z. Fujimoto, H. Noguchi, H. Habe, T. Omori, H. Yamane and H. Nojiri

Synopsis: The electron-transfer complex between the terminal oxygenase and ferredoxin of carbazole 1,9a-dioxygenase was crystallized and diffraction data were collected to 1.90 Å resolution.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 581-584  [ doi:10.1107/S1744309105014594 ]

A preliminary crystallographic analysis of the putative mevalonate diphosphate decarboxylase from Trypanosoma brucei

E. Byres, D. M. A. Martin and W. N. Hunter

Synopsis: The gene encoding the putative mevalonate diphosphate decarboxylase, an enzyme from the mevalonate pathway of isoprenoid precursor biosynthesis, has been cloned from T. brucei. Recombinant protein has been expressed, purified and highly ordered crystals obtained and characterized to aid the structure-function analysis of this enzyme.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 585-586  [ doi:10.1107/S1744309105015228 ]

Oligonucleotides with cyclohexene-nucleoside building blocks: crystallization and preliminary X-ray studies of a left-handed sequence GTGTACAC

K. Robeyns, P. Herdewijn and L. Van Meervelt

Synopsis: A novel oligonucleotide containing cyclohexene-nucleoside building blocks has been crystallized. Crystals belong to space group R3 and diffract to 1.7 Å.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 587-590  [ doi:10.1107/S1744309105015721 ]

Purification, crystallization and preliminary crystallographic analysis of the Hermes transposase

Z. N. Perez, P. Musingarimi, N. L. Craig, F. Dyda and A. B. Hickman

Synopsis: Upon purification, an N-terminally deleted version of the Hermes transposase exists in solution as a mixture of two species that are approximately hexameric and dimeric. Crystals have been obtained of the smaller species that diffract to 2.1 Å resolution.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 591-594  [ doi:10.1107/S1744309105015642 ]

Expression, purification, crystallization and preliminary X-ray analysis of a nucleoside kinase from the hyperthermophile Methanocaldococcus jannaschii

L. Arnfors, T. Hansen, W. Meining, P. Schönheit and R. Ladenstein

Synopsis: Nucleoside kinase from the hyperthermophilic archaeon M. jannaschii is a member of the PFK-B family which belongs to the ribokinase superfamily. Here, its expression, purification, crystallization and preliminary X-ray analysis are described.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 595-598  [ doi:10.1107/S1744309105015757 ]

Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis

H. Tamura, H. Matsumura, T. Inoue, H. Ashida, Y. Saito, A. Yokota and Y. Kai

Synopsis: Crystals of the 39 kDa functional form of methylthioribose-1-phosphate isomerase from B. subtilis diffracted to 2.50 Å.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 599-602  [ doi:10.1107/S1744309105015745 ]

Crystallization and preliminary X-ray analysis of the C-terminal cytoplasmic domain of FlhA, a membrane-protein subunit of the bacterial flagellar type III protein-export apparatus

Y. Saijo-Hamano, K. Imada, T. Minamino, M. Kihara, R. M. Macnab and K. Namba

Synopsis: Crystals of the cytoplasmic domain of FlhA, a membrane-protein component of the bacterial flagellar type III protein-export apparatus from Salmonella, have been obtained and characterized by X-ray diffraction.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 603-605  [ doi:10.1107/S1744309105015733 ]

Crystallization and preliminary X-ray analysis of molecular chaperone-like diol dehydratase-reactivating factor in ADP-bound and nucleotide-free forms

K. Mori, N. Hieda, M. Yamanishi, N. Shibata and T. Toraya

Synopsis: The molecular chaperone-like reactivating factor for adenosylcobalamin (coenzyme B₁₂) dependent diol dehydratase was crystallized in ADP-bound and nucleotide-free forms. Preliminary X-ray analysis indicated that crystals are orthorhombic and diffract to 2.0 Å.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 606-608  [ doi:10.1107/S1744309105016301 ]

Crystallization and preliminary crystallographic analysis of an aminoglycoside kinase from Legionella pneumophila

C. T. Lemke, J. Hwang, B. Xiong, N. P. Cianciotto and A. M. Berghuis

Synopsis: Two crystal forms of the antibiotic resistance enzyme APH(9)-Ia from L. pneumophila are reported.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 609-611  [ doi:10.1107/S1744309105016118 ]

Crystallization and avoiding the problem of hemihedral twinning in crystals of ¹-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus

E. Inagaki, H. Takahashi, C. Kuroishi and T. H. Tahirov

Synopsis: ¹-Pyrroline-5-carboxylate dehydrogenase from T. thermophilus has been crystallized in a citrate-bound form. The crystals exhibited hemihedral twinning and untwinned crystals were obtained by exclusion of lithium sulfate from the crystallization solution. Apo, NAD⁺ bound-, NADH bound- and glutamate-bound forms were also obtained and characterized by X-ray diffraction.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 612-613  [ doi:10.1107/S1744309105016325 ]

Crystallization and preliminary X-ray crystallographic analysis of rat calcineurin B homologous protein 1

Y. Naoe, K. Arita, H. Hashimoto, H. Kanazawa, M. Sato and T. Shimizu

Synopsis: Calcineurin B homologous protein 1 from rat was expressed in E. coli, purified and crystallized. A full set of X-ray diffraction data has been collected to 2.2 Å resolution using a synchrotron-radiation source.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 614-616  [ doi:10.1107/S1744309105016453 ]

Complex assembly, crystallization and preliminary X-ray crystallographic studies of rhesus macaque MHC Mamu-A*01 complexed with an immunodominant SIV-Gag nonapeptide

F. Chu, Z. Lou, B. Gao, J. I. Bell, Z. Rao and G. F. Gao

Synopsis: Crystallization of the first rhesus macaque MHC class I complex.

Online 1 June 2005

 

Acta Cryst. (2005). F61, 617-620  [ doi:10.1107/S1744309105013849 ]

Crystallization and preliminary X-ray diffraction analysis of the sialic acid-binding domain (VP8*) of porcine rotavirus strain CRW-8

S. A. Scott, G. Holloway, B. S. Coulson, A. J. Szyczew, M. J. Kiefel, M. von Itzstein and H. Blanchard

Synopsis: The sialic acid-binding domain (VP8*) component of the porcine CRW-8 rotavirus spike protein has been overexpressed in E. coli, purified and co-crystallized with an N-acetylneuraminic acid derivative. X-ray diffraction data have been collected to 2.3 Å, which has enabled determination of the structure by molecular replacement.

Online 1 June 2005