Structural Biology and Crystallization Communications

 

Acta Cryst. (2005). F61, 722-728  [ doi:10.1107/S1744309105022980 ]

Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus

E. Pleshe, J. Truesdell and R. T. Batey

Synopsis: The crystal structure of Aquifex aeolicus TrmH, a member of the a/b-knot superfamily responsible for O methylation of G18 of tRNAs, was determined to 1.85  Å resolution using the molecular-replacement method.

PDB reference: 1zjr

Online 30 July 2005

 

Acta Cryst. (2005). F61, 729-732  [ doi:10.1107/S1744309105019469 ]

Precipitation diagram and optimization of crystallization conditions at low ionic strength for deglycosylated dye-decolorizing peroxidase from a basidiomycete

S. Saijo, T. Sato, N. Tanaka, A. Ichiyanagi, Y. Sugano and M. Shoda

Synopsis: Precipitation phase diagrams can be rapidly constructed using dispensing-robot technology. These diagrams provide information that assists in optimization of crystal growth.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 733-736  [ doi:10.1107/S1744309105019640 ]

New tetragonal form of reaction centers from Rhodobacter sphaeroides and the involvement of a manganese ion at a crystal contact point

G. Uyeda, A. Cámara-Artigas, J. C. Williams and J. P. Allen

Synopsis: Reaction centers from R. sphaeroides have been crystallized in a new crystal form in the presence of manganese.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 737-739  [ doi:10.1107/S1744309105019779 ]

Cloning, purification and crystallization of Thermus thermophilus proline dehydrogenase

T. A. White and J. J. Tanner

Synopsis: Cloning, purification and crystallization of T. thermophilus proline dehydrogenase is reported. The detergent n-octyl -D-glucopyranoside was used to reduce polydispersity, which enabled crystallization.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 740-742  [ doi:10.1107/S1744309105019792 ]

Crystallization and preliminary X-ray crystallographic analysis of adenosine 5'-monophosphate deaminase (AMPD) from Arabidopsis thaliana in complex with coformycin 5'-phosphate

B. W. Han, C. A. Bingman, D. K. Mahnke, R. L. Sabina and G. N. Phillips

Synopsis: Adenosine 5'-monophosphate deaminase from A. thaliana has been crystallized in complex with coformycin 5'-phosphate. Diffraction data have been collected to 3.34  Å resolution.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 743-746  [ doi:10.1107/S1744309105019949 ]

Crystallization and preliminary X-ray analysis of binary and ternary complexes of Haloferax mediterranei glucose dehydrogenase

J. Esclapez, K. L. Britton, P. J. Baker, M. Fisher, C. Pire, J. Ferrer, M. J. Bonete and D. W. Rice

Synopsis: Single crystals of binary and ternary complexes of wild-type and D38C mutant H. mediterranei glucose dehydrogenase have been obtained by the hanging-drop vapour-diffusion method.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 747-749  [ doi:10.1107/S1744309105020518 ]

Crystallization and preliminary X-ray study of a family 10 alkali-thermostable xylanase from alkalophilic Bacillus sp. strain NG-27

K. Manikandan, A. Bhardwaj, A. Ghosh, V. S. Reddy and S. Ramakumar

Synopsis: A family 10 alkali-thermostable xylanase from Bacillus sp. NG-27 has been crystallized. A diffraction data set has been collected to 2.2  Å resolution.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 750-752  [ doi:10.1107/S1744309105020439 ]

Crystallization and preliminary X-ray diffraction analyses of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels

N. Suzuki, Y. Yamazaki, Z. Fujimoto, T. Morita and H. Mizuno

Synopsis: Crystals of pseudechetoxin and pseudecin, potent peptidic inhibitors of cyclic nucleotide-gated ion channels, have been prepared and X-ray diffraction data have been collected to 2.25 and 1.90  Å resolution, respectively.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 753-755  [ doi:10.1107/S1744309105020646 ]

Cloning, expression, purification and preliminary crystallographic data for Rv3214 (EntD), a predicted cofactor-dependent phosphoglycerate mutase from Mycobacterium tuberculosis

H. A. Watkins, M. Yu and E. N. Baker

Synopsis: A putative phosphoglycerate mutase from M. tuberculosis (Rv3214) has been crystallized. Diffraction data have been collected to 2.15  Å resolution from its selenomethionine-substituted form.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 756-758  [ doi:10.1107/S1744309105020737 ]

Crystallization and preliminary X-ray diffraction analysis of a flavoenzyme amine dehydrogenase/oxidase from Pyrococcus furiosus DSM 3638

P. J. Monaghan, D. Leys and N. S. Scrutton

Synopsis: This report describes the crystallization of a recombinant flavoprotein amine dehydrogenase/oxidase with specificity for L-proline from the hyperthermophile P. furiosus DSM 3638 and X-ray diffraction data collection. Crystals belonged to space group P1 and diffracted to a resolution of 3.3  Å.

Online 8 July 2005

 

Acta Cryst. (2005). F61, 759-761  [ doi:10.1107/S1744309105021093 ]

Crystallization and preliminary X-ray analysis of pyruvate kinase from Bacillus stearothermophilus

K. Suzuki, S. Ito, A. Shimizu-Ibuka and H. Sakai

Synopsis: This report describes the crystallization and X-ray diffraction data collection of three types (wild-type, W416F/V435W and C9S/C268S) of B. stearothermophilus. Crystals of C9S/C268S belonged to space group P6₂22 and diffracted to a resolution of 2.4  Å.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 762-765  [ doi:10.1107/S174430910502141X ]

Preliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2

N. G. A. Abrescia, H. M. Kivelä, J. M. Grimes, J. K. H. Bamford, D. H. Bamford and D. I. Stuart

Synopsis: The viral capsid protein P2 of bacteriophage PM2 has been crystallized. Preliminary X-ray analysis demonstrates the position and orientation of the two trimers in the asymmetric unit.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 766-768  [ doi:10.1107/S1744309105021421 ]

Crystallization and preliminary X-ray diffraction study of a cell-wall invertase from Arabidopsis thaliana

M. Verhaest, K. Le Roy, S. Sansen, B. De Coninck, W. Lammens, C. J. De Ranter, A. Van Laere, W. Van den Ende and A. Rabijns

Synopsis: Crystals suitable for structural analysis have been prepared from a cell-wall invertase from A. thaliana.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 769-772  [ doi:10.1107/S174430910502169X ]

Crystallization and preliminary X-ray analysis of Leishmania major glyoxalase I

A. Ariza, T. J. Vickers, N. Greig, A. H. Fairlamb and C. S. Bond

Synopsis: The detoxification enzyme glyoxalase I from L. major has been crystallized. Preliminary molecular-replacement calculations indicate the presence of three glyoxalase I dimers in the asymmetric unit.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 773-775  [ doi:10.1107/S1744309105022086 ]

High-resolution diffraction from crystals of a membrane-protein complex: bacterial outer membrane protein OmpC complexed with the antibacterial eukaryotic protein lactoferrin

N. Sundara Baalaji, K. R. Acharya, T. P. Singh and S. Krishnaswamy

Synopsis: Crystals of the complex formed between the bacterial membrane protein OmpC and the antibacterial protein lactoferrin suitable for high-resolution structure determination have been obtained. The crystals belong to the hexagonal space group P6, with unit-cell parameters a = b = 116.3, c = 152.4  Å.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 776-778  [ doi:10.1107/S1744309105022451 ]

Purification, crystallization and preliminary crystallographic analysis of Arabidopsis thaliana imidazoleglycerol-phosphate dehydratase

S. E. Glynn, P. J. Baker, S. E. Sedelnikova, C. W. Levy, H. F. Rodgers, J. Blank, T. R. Hawkes and D. W. Rice

Synopsis: Imidazoleglycerol-phosphate dehydratase from A. thaliana has been overexpressed, purified and crystallized and data have been collected to 3  Å resolution.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 779-781  [ doi:10.1107/S1744309105022669 ]

Crystallization and preliminary X-ray diffraction studies of two domains of a bilobed extra-cytoplasmic function sigma factor SigC from Mycobacterium tuberculosis

K. G. Thakur and B. Gopal

Synopsis: Expression, purification and preliminary X-ray diffraction studies of the two domains of the sigma factor SigC from M. tuberculosis are reported.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 782-784  [ doi:10.1107/S1744309105022839 ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of LysA (Rv1293) from Mycobacterium tuberculosis

G. Kefala, L. J. Perry and M. S. Weiss

Synopsis: M. tuberculosis diaminopimelate decarboxylase, the enzyme that catalyzes the final step of lysine biosynthesis, has been cloned, expressed, purified and crystallized in the absence of cofactor or substrate.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 785-787  [ doi:10.1107/S1744309105023006 ]

Purification, crystallization and preliminary crystallographic studies of a calmodulin-OLFp hybrid molecule

C.-L. Chyan, P.-C. Huang, T.-H. Lin, J.-W. Huang, S. S. Lin, H.- Huang and Y.-C. Chen

Synopsis: The hybrid moelcule of calmodulin and calmodulin-binding domain of olfactory nucleotide-gated ion-channel peptide (CaM-OLFp) was crystallized and preliminary analyzed using X-ray diffaction.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 788-790  [ doi:10.1107/S1744309105023080 ]

Crystallization of a 2:2 complex of granulocyte-colony stimulating factor (GCSF) with the ligand-binding region of the GCSF receptor

E. Honjo, T. Tamada, Y. Maeda, T. Koshiba, Y. Matsukura, T. Okamoto, M. Ishibashi, M. Tokunaga and R. Kuroki

Synopsis: A 2:2 complex of highly purified GCSF receptor (Ig-CRH) with GCSF was crystallized. The crystal diffracted to 2.8  Å resolution with sufficient quality for further structure determination.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 791-795  [ doi:10.1107/S1744309105023055 ]

Crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate

K. Pauwels, R. Loris, G. Vandenbussche, J.-M. Ruysschaert, L. Wyns and P. Van Gelder

Synopsis: Crystals of the lipase of B. glumae in complex with its specific foldase were obtained in two forms. Crystallization, crystal manipulation and preliminary X-ray diffraction analysis are described.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 796-798  [ doi:10.1107/S1744309105023225 ]

Expression, crystallization and preliminary diffraction studies of the Pseudomonas putida cytochrome P450cam operon repressor CamR

K. Maenaka, K. Fukushi, H. Aramaki and Y. Shirakihara

Synopsis: The P. putida cytochrome P450cam operon repressor CamR has been expressed in E. coli and crystallized in space group P2₁2₁2.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 799-801  [ doi:10.1107/S1744309105023365 ]

Crystallization of ¹-tetrahydrocannabinolic acid (THCA) synthase from Cannabis sativa

Y. Shoyama, A. Takeuchi, F. Taura, T. Tamada, M. Adachi, R. Kuroki, Y. Shoyama and S. Morimoto

Synopsis: ¹-Tetrahydrocannabinolic acid (THCA) synthase from C. sativa was crystallized. The crystal diffracted to 2.7  Å resolution with sufficient quality for further structure determination.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 802-805  [ doi:10.1107/S1744309105023523 ]

Crystallization and preliminary X-ray diffraction analysis of the haem-binding protein HemS from Yersinia enterocolitica

S. Schneider and M. Paoli

Synopsis: The haem binding protein HemS from Y. enterocolitica has been crystallized in complex with its ligand. The crystals diffracted X-rays to 2.6  Å in-house.

Online 30 July 2005

 

Acta Cryst. (2005). F61, 806-807  [ doi:10.1107/S1744309105023729 ]

Crystallization and preliminary X-ray diffraction studies of NP24-I, an isoform of a thaumatin-like protein from ripe tomato fruits

R. Ghosh and C. Chakrabarti

Synopsis: A thaumatin-like antifungal protein, NP24-I, has been isolated from ripe tomato fruits. It was crystallized by the vapour-diffusion method and data were collected to 2.45  Å. The structure was solved by molecular replacement.

Online 30 July 2005