Structural Biology and Crystallization Communications

 

Acta Cryst. (2005). F61, 808-811  [ doi:10.1107/S1744309105026047 ]

Structure of the complex of porcine pancreatic elastase with a trimacrocyclic peptide inhibitor FR901451

T. Kinoshita, T. Kitatani, M. Warizaya and T. Tada

Synopsis: The crystal structure of porcine pancreatic elastase complexed with a novel trimacrocyclic peptide inhibitor FR901451 was solved at 1.9  Å resolution.

PDB reference: 2cv3

Online 31 August 2005

 

Acta Cryst. (2005). F61, 812-817  [ doi:10.1107/S1744309105023845 ]

The structure at 2.5  Å resolution of human basophilic leukemia-expressed protein BLES03

E. Bitto, C. A. Bingman, H. Robinson, S. T. M. Allard, G. E. Wesenberg and G. N. Phillips

Synopsis: The crystal structure of the 27.5  kDa BLES03 protein was determined at 2.5  Å resolution. Despite having an undetectable sequence relationship, the structure adopts a fold similar to that of eukaryotic initiation factor 4E with minor variations.

PDB reference: 1ztp

Online 31 August 2005

 

Acta Cryst. (2005). F61, 818-820  [ doi:10.1107/S1744309105026126 ]

Deposit3D: a tool for automating structure depositions to the Protein Data Bank

J. Badger, J. Hendle, S. K. Burley and C. R. Kissinger

Synopsis: This paper describes a Python script that may be used to gather all required structure-annotation information into an mmCIF file for upload through the RCSB PDB ADIT structure-deposition interface.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 821-824  [ doi:10.1107/S1744309105023079 ]

Expression, purification and preliminary crystallographic analysis of 2,4-dihydroxy-hepta-2-ene-1,7-dioate aldolase (HpcH) from Escherichia coli C

D. Rea, V. Fülöp, T. D. H. Bugg and D. I. Roper

Synopsis: 2,4-Dihydroxy-hepta-2-ene-1,7-dioate aldolase from E. coli C has been purified and crystallized. Diffraction data were collected to 1.6  Å and structure determination by molecular replacement is in progress.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 825-827  [ doi:10.1107/S174430910502316X ]

Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

C. Tricot, S. van Aelst, R. Wattiez, M. Mergeay, V. Stalon and J. Wouters

Synopsis: Overexpression, purification and crystallization of C. metallidurans CopK allowed the collection of a complete data set to 2.2  Å resolution.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 828-830  [ doi:10.1107/S1744309105023808 ]

Crystallization and preliminary X-ray analysis of the YjgF/YER057c/UK114-family protein ST0811 from Sulfolobus tokodaii strain 7

T. Miyakawa, K.- Hatano, W. C. Lee, Y. Kato, Y. Sawano, F. Yumoto, K. Nagata and M. Tanokura

Synopsis: Overexpression, purification and crystallization of the YjgF/YER057c/UK114-family protein from S. tokodaii strain 7 allowed the collection of a complete data set to 2.0  Å resolution.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 831-833  [ doi:10.1107/S1744309105023602 ]

Crystallization and preliminary X-ray crystallographic analysis of human RGS10 complexed with Gi3

H. K. Lee, K. H. Rhee, C.-W. Kim, K. Y. Hwang and E. E. Kim

Synopsis: Human RGS10 and Gi3 have been overexpressed and purified. Their complex has been crystallized (space group P4₃2₁2 or P4₁2₁2, unit-cell parameters a = 99.88, b = 99.88, c = 144.59  Å, = = = 90°) and diffraction data have been collected to 2.5  Å resolution using synchrotron radiation.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 834-836  [ doi:10.1107/S1744309105024012 ]

Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens

E. Ficko-Blean and A. B. Boraston

Synopsis: Crystallization of a family 84 glycoside hydrolase, a putative virulence factor, secreted by C. perfringens is reported.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 837-840  [ doi:10.1107/S1744309105024206 ]

Crystallization and preliminary X-ray crystallographic studies of the alkanesulfonate FMN reductase from Escherichia coli

B. Gao, A. Bertrand, W. H. Boles, H. R. Ellis and T. C. Mallett

Synopsis: Crystallization of the native and SeMet FMN reductase protein of the E. coli alkanesulfonate monooxygenase two-component enzyme system is reported.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 841-843  [ doi:10.1107/S1744309105024462 ]

Crystallization and preliminary X-ray diffraction analysis of a new chitin-binding protein from Parkia platycephala seeds

B. S. Cavada, R. E. R. Castellón, G. G. Vasconcelos, B. A. M. Rocha, G. A. Bezerra, H. Debray, P. Delatorre, C. S. Nagano, M. Toyama, V. P. T. Pinto, F. B. M. B. Moreno, F. Canduri and W. F. de Azevedo

Synopsis: Crystals of P. platycephala chintinase/lectin (PPL-2) belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 55.19, b = 59.95, c = 76.60  Å. The preliminary cystal structure of PPL-2 was solved at a resolution of 1.73  Å by molecular replacement, presenting a correlation coefficient of 0.558 and an R factor of 0.439.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 844-847  [ doi:10.1107/S1744309105025649 ]

X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater

L. Satyanarayana, C. G. Suresh, A. Patel, S. Mishra and P. K. Ghosh

Synopsis: The protein C-phycocyanin, involved in photosynthesis, has been purified from three cyanobacterial species: Spirulina, Phormidium and Lyngbya. These three proteins have been crystallized and characterized using X-ray crystallography.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 848-851  [ doi:10.1107/S1744309105025856 ]

Preliminary crystallographic analysis of the Escherichia coli YeaZ protein using the anomalous signal of a gadolinium derivative

S. Jeudy, M. Stelter, B. Coutard, R. Kahn and C. Abergel

Synopsis: The expression and crystallization of recombinant E. coli YeaZ protein, which belongs to a family of proteins of unknown function that are conserved in Gram-positive and Gram-negative bacteria, is reported. Phasing of the diffraction data was performed by the MAD method using the anomalous signal of a gadolinium complex, Gd-DOTMA.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 852-854  [ doi:10.1107/S1744309105026709 ]

Crystallization and preliminary crystallographic analysis of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica

G. S. Kachalova, R. I. Artyukh, N. V. Lavrova, E. M. Ryazanova, A. S. Karyagina, E. A. Kubareva and H. D. Bartunik

Synopsis: The (cytosine-5)-DNA methyltransferase NlaX from N. lactamica has been crystallized in space group P321, with unit-cell parameters a = 121.98, b = 121.98, c = 56.71  Å.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 855-857  [ doi:10.1107/S1744309105025789 ]

Crystallization and preliminary X-ray diffraction analysis of central structure domains from mumps virus F protein

Y. Liu, Y. Xu, J. Zhu, B. Qiu, Z. Rao, G. F. Gao and P. Tien

Synopsis: Single crystals of the central structure domains from mumps virus F protein have been obtained by the hanging-drop vapour-diffusion method. A diffraction data set has been collected to 2.2  Å resolution.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 858-860  [ doi:10.1107/S1744309105026783 ]

Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1

N. Kulahin, C. Kasper, O. Kristensen, J. S. Kastrup, V. Berezin, E. Bock and M. Gajhede

Synopsis: Mouse L1 modules Ig I-IV and F3 I-III were crystallized. The crystals diffracted X-rays to 3.5 and 2.8  Å resolution, respectively.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 861-863  [ doi:10.1107/S1744309105027004 ]

Expression, purification and crystallization of the ammonium transporter Amt-1 from Archaeoglobus fulgidus

S. L. A. Andrade, A. Dickmanns, R. Ficner and O. Einsle

Synopsis: The ammonium transporter Amt-1 from the cytoplasmic membrane of the hyperthermophilic archaeon A. fulgidus has been purified and crystallized.

Online 31 August 2005

 

Acta Cryst. (2005). F61, 864-866  [ doi:10.1107/S1744309105027028 ]

Purification, crystallization and preliminary X-ray analysis of a hexameric -glucosidase from wheat

M. Sue, K. Yamazaki, J.- Kouyama, Y. Sasaki, K. Ohsawa, T. Miyamoto, H. Iwamura and S. Yajima

Synopsis: Recombinant -glucosidase from wheat seedlings complexed with a substrate aglycone has been crystallized in a hexameric active form. A diffraction data set has been collected at 1.7  Å.

Online 31 August 2005