Structural Biology and Crystallization Communications

 

Acta Cryst. (2006). F62, 1-5  [ doi:10.1107/S1744309105040984 ]

Atomic resolution structure of the double mutant (K53,56M) of bovine pancreatic phospholipase A₂

K. Sekar, M. Yogavel, D. Gayathri, D. Velmurugan, R. Krishna, M.-J. Poi, Z. Dauter, M. Dauter and M.-D. Tsai

Synopsis: The atomic resolution crystal structure of the double mutant (K53,56M) of bovine pancreatic phospholipase A₂ is reported.

PDB reference: 2bax

Online 16 December 2005

 

Acta Cryst. (2006). F62, 6-9  [ doi:10.1107/S1744309105038248 ]

Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II

M. Budayova-Spano, S. Z. Fisher, M.-T. Dauvergne, M. Agbandje-McKenna, D. N. Silverman, D. A. A. Myles and R. McKenna

Synopsis: This article reports the production, crystallization and X-ray structure determination of perdeuterated human carbonic anhydrase (HCA II). The refined structure is shown to be highly isomorphous with hydrogenated HCA II, especially with regard to the active site architecture and solvent network.

PDB reference: 2ax2

Online 16 December 2005

 

Acta Cryst. (2006). F62, 10-12  [ doi:10.1107/S1744309105038534 ]

Crystallization and preliminary X-ray crystallographic analysis of the cysteine protease inhibitor clitocypin

K. Galesa, J. Brzin, J. Sabotic and D. Turk

Synopsis: Clitocypin is a cysteine protease inhibitor from the mushroom Clitocybe nebularis. The protein has been purified from natural sources and crystallized in a variety of non-isomorphous forms belonging to monoclinic and triclinic space groups.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 13-15  [ doi:10.1107/S1744309105038704 ]

X-ray crystallographic characterization of rhesus macaque MHC Mamu-A*02 complexed with an immunodominant SIV-Gag nonapeptide

Y. Feng, J. Qi, H. Zhang, J. Wang, J. Liu, F. Jiang and F. Gao

Synopsis: X-ray crystallographic characterization of rhesus macaque MHC Mamu-A*02 complexed with an immunodominant SIV-Gag nonapeptide.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 16-19  [ doi:10.1107/S1744309105038996 ]

Expression, purification, crystallization and preliminary X-ray analysis of the olfactomedin domain from the sea urchin cell-adhesion protein amassin

B. J. Hillier, V. Sundaresan, C. D. Stout and V. D. Vacquier

Synopsis: The olfactomedin (OLF) domain from the sea urchin cell-adhesion protein amassin has been crystallized. A native data set extending to 2.7  Å has been collected using an in-house X-ray source.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 20-22  [ doi:10.1107/S174430910503900X ]

Crystallization and preliminary crystallographic analysis of endo-1,3--glucanase from alkaliphilic Nocardiopsis sp. strain F96

G. Fibriansah, S. Masuda, R. Hirose, K. Hamada, N. Tanaka, S. Nakamura and T. Kumasaka

Synopsis: The endo-1,3--glucanase from alkaliphilic Nocardiopsis sp. strain F96 has been crystallized by the hanging-drop vapour-diffusion method. Diffraction data have been collected to 1.3  Å resolution.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 23-25  [ doi:10.1107/S1744309105039242 ]

Crystallization and preliminary X-ray crystallographic study of the extracellular domain of the 4-1BB ligand, a member of the TNF family

J.-S. Byun, D.-U. Kim, B. Ahn, B. S. Kwon and H.-S. Cho

Synopsis: The extracellular domain of the 4-1BB ligand fused with glutathione-S-transferase was expressed in Escherichia coli (Origami) and purified by using affinity and ion-exchange column chromatographic methods. Crystals of the 4-1BB ligand were obtained at 290  K by the hanging-drop vapour-diffusion method.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 26-28  [ doi:10.1107/S1744309105039618 ]

Crystallization and preliminary X-ray diffraction analysis of the arginine repressor of the hyperthermophile Thermotoga neapolitana

J. Massant, E. Peeters, D. Charlier and D. Maes

Synopsis: The arginine repressor of the hyperthermophile T. neapolitana was crystallized with and without its corepressor arginine. Both crystals diffracted to high resolution and belong to the orthorhombic space group P2₁2₁2₁, with similar unit-cell parameters.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 29-31  [ doi:10.1107/S1744309105039679 ]

Purification, crystallization and preliminary X-ray analysis of the catalytic domain of the Escherichia coli tRNase colicin D

K. Takahashi, T. Ogawa, M. Hidaka, K. Ohsawa, H. Masaki and S. Yajima

Synopsis: The tRNase domain of colicin D, which is specific to tRNA^Args, has been crystallized. A diffraction data set has been collected to a resolution of 1.05  Å.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 32-35  [ doi:10.1107/S1744309105039813 ]

Crystallization of a newly discovered histidine acid phosphatase from Francisella tularensis

R. L. Felts, T. J. Reilly, M. J. Calcutt and J. J. Tanner

Synopsis: A histidine acid phosphatase from the CDC Category A pathogen F. tularensis has been crystallized in space group P4₁2₁2, with unit-cell parameters a = 61.96, c = 210.78  Å. A 1.75  Å resolution data set was collected at Advanced Light Source beamline 4.2.2.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 36-38  [ doi:10.1107/S174430910503976X ]

Purification, crystallization and X-ray diffraction analysis of dihydropyrimidinase from Dictyostelium discoideum

B. Lohkamp, B. Andersen, J. Piskur and D. Dobritzsch

Synopsis: Dihydropyrimidinase from the slime mould D. discoideum was crystallized. A single crystal was shown to belong to space group I222 and diffracted anisotropically to better than 1.8  Å.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 39-43  [ doi:10.1107/S1744309105040303 ]

Crystallization of a functionally intact Hsc70 chaperone

J. Jiang, E. M. Lafer and R. Sousa

Synopsis: Success in crystallization of a functionally intact Hsp70 chaperone required genetic engineering to minimize polydispersity and modulate interdomain interactions, as well as high concentrations of the potent structure stabilizer TMAO. These approaches may be generally useful in crystallization of conformationally flexible proteins that exhibit interdomain motions.

PDB reference: 1yuw

Online 16 December 2005

 

Acta Cryst. (2006). F62, 44-46  [ doi:10.1107/S1744309105040297 ]

Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription elongation factors GreB from Escherichia coli and Gfh1 from Thermus thermophilus

A. A. Perederina, M. N. Vassylyeva, I. A. Berezin, V. Svetlov, I. Artsimovitch and D. G. Vassylyev

Synopsis: The GreB transcription cleavage factor of E. coli and the Gfh1 transcription elongation factor of T. thermophilus were cloned, expressed, purified and crystallized. Complete diffraction data sets were collected for the GreB and Gfh1 crystals to 2.6 and 2.8  Å resolution, respectively. Structure determination of these proteins is now in progress.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 47-48  [ doi:10.1107/S1744309105040613 ]

Crystallization and preliminary X-ray analysis of PH1566, a putative ribosomal RNA-processing factor from the hyperthermophilic archaeon Pyrococcus horikoshii OT3

M. Z. Jia, J. Ohtsuka, W. C. Lee, K. Nagata and M. Tanokura

Synopsis: A putative ribosomal RNA-processing factor consisting of two KH domains from Pyrococcus horikoshii OT3 (PH1566; 25  kDa) was crystallized by the sitting-drop vapour-diffusion method using PEG 3000 as the precipitant. The space group of the crystals was determined as primitive orthorhombic P2₁2₁2₁, with unit-cell parameters a = 45.9, b = 47.4, c = 95.7  Å.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 49-51  [ doi:10.1107/S1744309105040893 ]

Crystallization, preliminary X-ray diffraction and structure solution of MosA, a dihydrodipicolinate synthase from Sinorhizobium meliloti L5-30

Y. A. Leduc, C. P. Phenix, J. Puttick, K. Nienaber, D. R. J. Palmer and L. T. J. Delbaere

Synopsis: MosA from S. meliloti L5-30 has been crystallized in solution with pyruvate and the 2.3  Å resolution structure has been solved by molecular replacement using E. coli dihydrodipicolinate synthase as the model.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 52-55  [ doi:10.1107/S1744309105041023 ]

Expression, refolding and crystallizations of the Grb2-like (GADS) C-terminal SH3 domain complexed with a SLP-76 motif peptide

A. Faravelli and N. Dimasi

Synopsis: Several crystals of the Grb2-like C-terminal SH3 domain in complex with a motif peptide from the SLP-76 protein were obtained and characterized.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 56-60  [ doi:10.1107/S1744309105041394 ]

Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens

F. Huché, P. Delepelaire, C. Wandersman and W. Welte

Synopsis: The expression, purification, and crystallization in space group P2₁2₁2₁ of the complex HasA-HasR from S. marcescens are reported. Diffraction data have been collected and processed to 6.8  Å.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 61-66  [ doi:10.1107/S1744309105041400 ]

Expression, purification, crystallization and preliminary X-ray analysis of the human RuvB-like protein RuvBL1

S. Gorynia, P. M. Matias, S. Gonçalves, R. Coelho, G. Lopes, M. Thomaz, M. Huber, B. Haendler, P. Donner and M. A. Carrondo

Synopsis: Human RuvB-like protein RuvBL1 plays important roles in essential signaling pathways like c-Myc and Wnt, in transcription, and in DNA repair and apoptosis. Crystals of both native and a Se-Met derivative were obtained and characterized. SAD data leading to the structure solution at 2.2  Å were measured from the Se-Met crystals.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 67-69  [ doi:10.1107/S1744309105041461 ]

Crystallization and preliminary X-ray diffraction analysis of human phosphate-binding protein

C. Contreras-Martel, P. Carpentier, R. Morales, F. Renault, M.-L. Chesne-Seck, D. Rochu, P. Masson, J. C. Fontecilla-Camps and E. Chabrière

Synopsis: The purification, detergent-exchange protocol and crystallization conditions that led to the discovery of HPBP are reported. HPBP is a new human apoprotein that is absent from the genomic database and is the first phosphate transporter characterized in human plasma.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 70-73  [ doi:10.1107/S1744309105041473 ]

The production, purification and crystallization of a soluble form of the nonclassical MHC HLA-G: the essential role of cobalt

C. S. Clements, L. Kjer-Nielsen, L. Kostenko, J. McCluskey and J. Rossjohn

Synopsis: X-ray diffraction data were collected to 1.9  Å from crystals of HLA-G. Cobalt ions were found to be essential for the production of diffracting crystals.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 74-76  [ doi:10.1107/S1744309105041758 ]

Overexpression, purification, crystallization and preliminary diffraction studies of the Protaminobacter rubrum sucrose isomerase SmuA

S. Ravaud, H. Watzlawick, R. Haser, R. Mattes and N. Aghajari

Synopsis: The P. rubrum sucrose isomerase SmuA, a key enzyme in the industrial production of isomaltulose, was crystallized and diffraction data were collected to 1.95  Å resolution.

Online 16 December 2005

 

Acta Cryst. (2006). F62, 77-79  [ doi:10.1107/S1744309105041199 ]

Crystallization and preliminary X-ray diffraction analysis of a cold-adapted catalase from Vibrio salmonicida

E. K. Riise, M. S. Lorentzen, R. Helland and N. P. Willassen

Synopsis: Monoclinic (P2₁) crystals of a His-tagged form of V. salmonicida catalase without cofactor diffract X-rays to 1.96  Å.

Online 23 December 2005

 

Acta Cryst. (2006). F62, 80-82  [ doi:10.1107/S174430910504145X ]

Crystallization and preliminary crystallographic analysis of human common-type acylphosphatase

R. C. Y. Yeung, S. Y. Lam and K.-B. Wong

Synopsis: Human common-type acylphosphatase was crystallized in space group P2₁2₁2₁, with unit-cell parameters a = 42.58, b = 47.23, c = 57.26  Å. A complete diffraction data set was collected to 1.45  Å.

Online 23 December 2005

 

Acta Cryst. (2006). F62, 83-87  [ doi:10.1107/S1744309105042958 ]

Notes for authors 2006

Online 23 December 2005