Structural Biology and Crystallization Communications

 

Acta Cryst. (2006). F62, 88-93  [ doi:10.1107/S1744309105042491 ]

Structure of human salivary -amylase crystallized in a C-centered monoclinic space group

S. Z. Fisher, L. Govindasamy, C. Tu, M. Agbandje-McKenna, D. N. Silverman, H. J. Rajaniemi and R. McKenna

Synopsis: Here, the serendipitous purification, crystallization and ultimate structure determination of a HSA non-crystallographic symmetry (NCS) dimer, while attempting to purify human carbonic anhydrase VI (HCA VI) from saliva using an affinity resin for -class carbonic anhydrases, is presented.

PDB reference: 1xv8

Online 27 January 2006

 

Acta Cryst. (2006). F62, 94-96  [ doi:10.1107/S1744309105040698 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of rice lectin from Oryza sativa

Y.-C. Huang, Y.-H. Lin, C.-H. Shih, C.-L. Shih, T. Chang and C.-J. Chen

Synopsis: Rice lectin was crystallized and analyzed by X-ray crystallography.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 97-99  [ doi:10.1107/S1744309105041011 ]

Crystallization and preliminary crystallographic study of the yeast Malassezia sympodialis allergen Mala s 1

M. Vilhelmsson, B. M. Hallberg, O. Rasool, A. Zargari, A. Scheynius and A. Achour

Synopsis: Crystals of the M. sympodialis allergen Mala s 1 have been obtained using the hanging-drop vapour-diffusion method. A diffraction data set has been collected from native crystals to 1.35  Å resolution.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 100-103  [ doi:10.1107/S1744309105042582 ]

Crystallization and preliminary X-ray diffraction studies of two thermostable -galactosidases from glycoside hydrolase family 36

M. Foucault, H. Watzlawick, R. Mattes, R. Haser and P. Gouet

Synopsis: The -galactosidases AgaA, AgaB and AgaA A355E mutant from Geobacillus stearothermophilus have been overexpressed in Escherichia coli. Crystals of AgaB and AgaA A355E have been obtained by the vapour-diffusion method and synchrotron data have been collected to 2.0 and 2.8  Å resolution, respectively.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 104-105  [ doi:10.1107/S1744309105042508 ]

Crystallization and preliminary X-ray analysis of cytosolic -mannosidase from Thermotoga maritima

M. Nakajima, S. Fushinobu, H. Imamura, H. Shoun and T. Wakagi

Synopsis: Cytosolic class II -mannosidase from T. maritima (TM1851), a family 38 glycoside hydrolase, was crystallized. A diffraction data set was collected to 2.9  Å resolution.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 106-109  [ doi:10.1107/S1744309105042764 ]

Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin

V. E. Syakhovich, N. T. Saraswathi, M. Ruff, S. B. Bokut and D. Moras

Synopsis: Non enzymatic modification of haemoglobin by glucose plays an important role in diabetes pathogenesis. Here the purification, characterization and crystallization of human glycosylated haemoglobin are reported.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 110-112  [ doi:10.1107/S174430910504306X ]

Crystallization of the NADH-oxidizing domain of the Na⁺-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae

M. Tao, K. Türk, J. Diez, M. G. Grütter, G. Fritz and J. Steuber

Synopsis: The FAD domain of the NqrF subunit from the Na⁺-translocating NADH dehydrogenase from V. cholerae has been purified and crystallized. A complete data set was recorded at 3.1  Å.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 113-116  [ doi:10.1107/S1744309105042995 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of mammalian MSS4-Rab8 GTPase protein complex

A. Itzen, N. Bleimling, A. Ignatev, O. Pylypenko and A. Rak

Synopsis: The MSS4 (mammalian suppressor of Sec4) protein in complex with nucleotide-free Rab8 GTPase has been purified and crystallized in a form suitable for structure analysis and a complete data set has been collected to 2  Å resolution.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 117-120  [ doi:10.1107/S1744309105043186 ]

Crystallization and preliminary characterization of three different crystal forms of human saposin C heterologously expressed in Pichia pastoris

R. Schultz-Heienbrok, N. Remmel, R. Klingenstein, M. Rossocha, K. Sandhoff, W. Saenger and T. Maier

Synopsis: Three different crystal forms were obtained of human saposin C. The structures could not be determined by molecular replacement using known solution structures of the protein as search models, supporting the notion of a highly flexible protein.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 121-124  [ doi:10.1107/S1744309105043198 ]

Crystallization and preliminary X-ray diffraction analysis of CfaE, the adhesive subunit of the CFA/I fimbriae from human enterotoxigenic Escherichia coli

Y.-F. Li, S. Poole, F. Rasulova, L. Esser, S. J. Savarino and D. Xia

Synopsis: The adhesin CfaE of the CFA/I fimbriae from human enterotoxigenic E. coli has been crystallized. CfaE crystals diffracted X-rays to better than 2.4  Å and phasing was solved by the SIRAS method.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 125-127  [ doi:10.1107/S174430910504323X ]

Crystallization and preliminary crystallographic analysis of the catechol 2,3-dioxygenase PheB from Bacillus stearothermophilus BR219

K. Sugimoto, K. Matsufuzi, H. Ohnuma, M. Senda, M. Fukuda and T. Senda

Synopsis: PheB, an extradiol-cleaving catecholic dioxygenase, was crystallized by the hanging-drop vapour-diffusion method using PEG 4000 as a precipitant. The crystal belongs to the orthorhombic system, space group P2₁2₁2₁, and diffracts to 2.3  Å resolution.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 128-132  [ doi:10.1107/S1744309105043228 ]

Crystallization and preliminary crystallographic studies of human septin 1 with site-directed mutations

H. Hu, W. Yu, S. Li, X. Ding, L. Yu and R.-C. Bi

Synopsis: The homogeneity of septin 1 has been improved by site-directed mutation of serine residues and only a small alteration in the secondary structure is observed to arise from the mutations. Crystals of the septin 1 mutant were grown and diffraction data were collected to 2.5  Å resolution.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 133-135  [ doi:10.1107/S1744309106000030 ]

Crystallization of the C-terminal domain of the mouse brain cytosolic long-chain acyl-CoA thioesterase

R. Serek, J. K. Forwood, D. A. Hume, J. L. Martin and B. Kobe

Synopsis: The C-terminal domain of the mouse long-chain acyl-CoA thioesterase has been expressed in bacteria and crystallized by vapour diffusion. The crystals diffract to 2.4  Å resolution.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 136-138  [ doi:10.1107/S1744309106000637 ]

Cloning, expression, purification, crystallization and preliminary X-ray studies of epoxide hydrolases A and B from Mycobacterium tuberculosis

B. K. Biswal, G. Garen, M. M. Cherney, C. Garen and M. N. G. James

Synopsis: Epoxide hydrolases A (Rv3617) and B (Rv1938), detoxification enzymes from M. tuberculosis, have been cloned, expressed, purified and crystallized. Crystals of Rv3617 and Rv1938 diffracted to 3.0 and 2.1  Å resolution, respectively.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 139-141  [ doi:10.1107/S1744309106000649 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of erythronate-4-phosphate dehydrogenase from Pseudomonas aeruginosa

J. Y. Ha, J. H. Lee, K. H. Kim, D. J. Kim, H. H. Lee, H.-K. Kim, H.-J. Yoon and S. W. Suh

Synopsis: Erythronate-4-phosphate dehydrogenase from P. aeruginosa was crystallized and X-ray diffraction data were collected to 2.20  Å resolution.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 142-144  [ doi:10.1107/S1744309106000844 ]

Crystallization and preliminary X-ray crystallographic analysis of the -2,6-sialyltransferase PM0188 from Pasteurella multosida

D.-U. Kim, J.-H. Yoo, K. Ryu and H.-S. Cho

Synopsis: The -2,6-sialyltransferase PM0188 from P. multocida was purified using affinity-column chromatographic methods and crystallized using the hanging-drop vapour-diffusion method at 293  K.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 145-147  [ doi:10.1107/S1744309106000832 ]

Crystallization and preliminary X-ray crystallographic analysis of EstE1, a new and thermostable esterase cloned from a metagenomic library

J.-S. Byun, J.-K. Rhee, D.-U. Kim, J.-W. Oh and H.-S. Cho

Synopsis: Recombinant EstE1 protein with a histidine tag at the C-terminus was overexpressed in Escherichia coli strain BL21(DE3) and then purified by affinity chromatography. The protein was then crystallized at 290  K by the hanging-drop vapour-diffusion method.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 148-152  [ doi:10.1107/S1744309106000996 ]

Crystallization and preliminary X-ray diffraction analysis of the magnesium transporter CorA

J. Payandeh and E. F. Pai

Synopsis: Full-length and soluble domains of the integral membrane protein CorA have been expressed, purified and crystallized. X-ray diffraction data have been collected and analyzed.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 153-156  [ doi:10.1107/S1744309106001229 ]

In-house SIRAS phasing of the polyunsaturated fatty-acid isomerase from Propionibacterium acnes

A. Liavonchanka, E. Hornung, I. Feussner and M. Rudolph

Synopsis: Low iodide concentrations were sufficient to allow SAD and SIRAS phasing of cubic crystals of a novel fatty acid isomerase using Cu  K radiation.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 157-162  [ doi:10.1107/S1744309106001199 ]

Purification and crystallization of dengue and West Nile virus NS2B-NS3 complexes

A. D'Arcy, M. Chaillet, N. Schiering, F. Villard, S. P. Lim, P. Lefeuvre and P. Erbel

Synopsis: Crystals of dengue serotype 2 and West Nile virus NS2B-NS3 protease complexes have been obtained and the crystals of both diffract to useful resolution. Sample homogeneity was essential for obtaining X-ray-quality crystals of the dengue protease. Controlled proteolysis produced a crystallizable fragment of the apo West Nile virus NS2B-NS3 and crystals were also obtained in the presence of a peptidic inhibitor.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 163-165  [ doi:10.1107/S1744309106001448 ]

Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab

V. Lavanya Latha, K. A. Kulkarni, R. Nagender Rao, N. Siva Kumar and K. Suguna

Synopsis: The galactose-specific lectin from the seeds of a leguminous plant, D. lablab, has been crystallized. Molecular-replacement solution using 3.0  Å X-ray diffraction data showed the lectin to be a tetramer.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 166-168  [ doi:10.1107/S1744309106001801 ]

Crystallization and preliminary X-ray diffraction analysis of the lectin from Dioclea rostrata Benth seeds

P. Delatorre, K. S. Nascimento, L. M. Melo, E. P. de Souza, B. A. M. da Rocha, R. G. Benevides, T. M. de Oliveira, G. A. Bezerra, M. J. B. Bezerra, R. M. S. da Cunha, F. A. B. da Cunha, V. N. Freire and B. S. Cavada

Synopsis: D. rostrata lectin was crystallized by hanging-drop vapor diffusion. The crystal belongs to the orthorhombic space group I222 and diffracted to 1.87  Å resolution.

Online 27 January 2006

    

Acta Cryst. (2006). F62, 169-171  [ doi:10.1107/S1744309106001813 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii

E. Inagaki, K. Sakamoto, N. Obayashi, T. Terada, M. Shirouzu, Y. Bessho, C. Kuroishi, S. Kuramitsu, A. Shinkai and S. Yokoyama

Synopsis: Galactokinase from P. horikoshii has been crystallized in both the apo form and as a ternary complex with -D-galactose and an ATP analogue. The crystals were characterized by X-ray diffraction. The kinetic parameters of the enzyme were determined.

Online 27 January 2006

 

Acta Cryst. (2006). F62, 172-174  [ doi:10.1107/S1744309106002053 ]

Preliminary crystallographic studies of yeast mitochondrial peripheral membrane protein Tim44p

R. Josyula, Z. Jin, D. McCombs, L. DeLucas and B. Sha

Synopsis: Tim44p is an essential mitochondrial peripheral membrane protein. To investigate the mechanism by which Tim44p functions in the TIM23 translocon to deliver the mitochondrial protein precursors, the yeast Tim44p has been crystallized.

Online 27 January 2006