Received 16 February 2006
Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.
Quinoproteins constitute a class of proteins that use one of five different quinone-containing cofactors for the two-electron oxidation of alcohols and amines. Quinoproteins containing pyrroloquinoline quinone (PQQ) are present in a variety of different prokaryotes, including Gram-negative and Gram-positive bacteria and archaea (Oubrie, 2003), but the alleged presence (Kasahara & Kato, 2003) of PQQ-dependent dehydrogenases in eukaryotes is controversial (Felton & Anthony, 2005; Kasahara & Kato, 2005; Rucker et al., 2005).
The PQQ-dependent soluble glucose dehydrogenase (sGDH) from the Gram-negative bacterium Acinetobacter calcoaceticus is the prototype of a subclass of enzymes with a six-bladed -propeller structure (Oubrie, 2003) and the only protein of its class for which a detailed biochemical characterization has been carried out. The enzyme functions as a homodimer of 100 kDa (454 amino acids per monomer after cleavage of the signal peptide that directs it to the periplasm) and binds three calcium ions and one PQQ per subunit (Olsthoorn & Duine, 1996; Olsthoorn et al., 1997; Oubrie, Rozeboom, Kalk, Duine et al., 1999; Oubrie, Rozeboom, Kalk, Olsthoon et al., 1999). The sGDH catalyzes the conversion of monosaccharides and disaccharides as well as the trisaccharide maltotriose into their corresponding lactones (Olsthoorn & Duine, 1998; Igarashi et al., 2004). The kinetic mechanism of the reductive half-reaction includes an initial reversible fast step leading to formation of a fluorescing intermediate followed by an irreversible and rate-limiting step (Olsthoorn & Duine, 1998). Crystal structures have been determined of the sGDH in different states, i.e. in the apo form (Oubrie, Rozeboom, Kalk, Duine et al., 1999), in complex with PQQ (Oubrie, Rozeboom, Kalk, Olsthoon et al., 1999) and in ternary complexes with PQQ and the substrate glucose (Oubrie, Rozeboom, Kalk, Olsthoon et al., 1999) or the inhibitor methylhydrazine (Oubrie, Rozeboom & Dijkstra, 1999). The combined structural and biochemical data are consistent with a catalytic mechanism involving direct hydride transfer (Oubrie, Rozeboom, Kalk, Olsthoon et al., 1999).
The enzyme has been implemented on test strips that are used by diabetics to monitor their blood glucose levels. Improvement of the substrate specificity of the sGDH could improve the reliability of these strips, as high levels of maltose in blood, only relevant in some specific clinical conditions, can interfere with the glucose diagnostics (Wens et al., 1998). For this purpose, three generations of sGDH variants with successively reduced activity towards maltose have been generated. Here, we describe an efficient and reproducible microseeding method that was used to grow crystals of each of the three variants. Using the same method, we also succeeded in growing crystals of the sGDH homologues from Escherichia coli K12 (Gene ID 945467) and Streptomyces coelicolor A32(2) (Gene ID 1100948).
Samples of wild-type and three variants of A. calcoaceticus sGDH were generously supplied by Roche Diagnostics (Penzberg, Germany) as a freeze-dried powder. The variants are called mutant A (T348G), mutant B (T348G, N428P) and mutant G (Y171G, E245D, M341V, T348G, N428P) (Kratzsch et al., 2002). Before use, samples were resuspended in a buffer solution containing 3 mM CaCl2, 120 mM NaCl, 10 mM HEPES pH 7.5. Methods to clone, express and purify the sGDH homologues from E. coli and S. coelicolor as well as a biochemical characterization of these proteins will be published elsewhere.
To obtain microseeds, a single crystal was transferred to an Eppendorf cup containing 5 µl of a stabilizing solution containing 30% PEG 6000, 3 mM CaCl2, 120 mM NaCl and 50 mM CHES pH 9.2. The crystal was crushed manually using a polypropylene pellet pestle (Sigma) while keeping the sample on ice. The sample was then diluted with the stabilizing solution to an end volume of 200 µl to obtain the 1/1 (or 100) stock. Serial dilutions up to 1/106 were produced and stored at 277 K. Microseeds of E. coli and S. coelicolor sGDH homologues were produced in the same way using stabilizing solutions buffered with 100 mM of either CHES pH 9.2 or HEPES pH 7.5.
Crystallization conditions were screened using the hanging-drop vapour-diffusion method (Q Plate II, Hampton Research) at 289 K. A resuspended protein solution (5 mg ml-1) was mixed with an equal volume of precipitant solution (17-22% PEG 6000, 1 mM CaCl2, 120 mM NaCl, CHES pH 9.2). A total of eight drops of 2 µl of this mixture were placed on a cover slip. To seven of these drops, 1 µl of one of the seven seed-stock solutions (100 to 106 dilution series) was added, whilst 1 µl stabilizing solution was added to the last drop as a control to check for crystallization in the absence of microseeds. The drops were equilibrated against a 1 ml reservoir of precipitant solution. The sGDH homologues from E. coli and S. coelicolor were screened using wild-type seeds in the same way, but a precipitant solution buffered at a different pH (17-22% PEG 6000, 1 mM CaCl2, 120 mM NaCl, 100 mM HEPES pH 7.5) was also used.
Crystals of A. calcoaceticus sGDH variants were flash-frozen in a solution containing 20%(v/v) glycerol or ethylene glycol in addition to 30%(w/v) PEG 6000, 120 mM NaCl, 3 mM CaCl2, 100 mM CHES pH 9.2. Crystals of the E. coli sGDH homologue were cryocooled in a solution containing 20%(v/v) ethylene glycol in addition to 30%(w/v) PEG 6000, 120 mM NaCl, 3 mM CaCl2, 100 mM CHES pH 9.2, while crystals of the S. coelicolor homologue were frozen in a cryoprotectant containing 20%(v/v) glycerol in addition to 30%(w/v) PEG 6000, 120 mM NaCl, 3 mM CaCl2, 100 mM HEPES pH 7.5. Data sets were collected at station XRD1 of the ELETTRA synchrotron-radiation source in Trieste, Italy, at station 14.2 at the Synchrotron Light Source (SLS) in Daresbury, United Kingdom and at station BL-2 of the Protein Structure Factory at BESSY in Berlin, Germany. Data sets were processed and reduced using either the HKL2000 package (Otwinowski & Minor, 1997) or MOSFLM (Leslie, 1992) and programs from the CCP4 suite (Collaborative Computational Project, Number 4, 1994).
Crystals of wild-type sGDH were previously obtained using the hanging-drop vapour-diffusion method with a reservoir solution containing 16-23% PEG 6000, 3 mM CaCl2, 120 mM NaCl, 100 mM Tris-glycine pH 9.2 (Oubrie, Rozeboom, Kalk, Olsthoon et al., 1999). These crystallization conditions were also screened for the sGDH variants, but results were erratic in terms of reproducibility and crystal morphology. A second drawback of these conditions is the presence of Tris, which for reasons that are only poorly understood prevents reconstitution of the holoenzyme in the crystals upon soaking with the PQQ cofactor. In order to obtain structural information on cofactor- and substrate-bound states, it has therefore been found necessary to transfer the crystals to a buffer containing CHES (Oubrie, Rozeboom, Kalk, Duine et al., 1999; Oubrie, Rozeboom & Dijkstra, 1999). Unfortunately, few crystals survive this treatment and crystallization in a buffer containing CHES has so far been unsuccessful, even with wild-type A. calcoaceticus protein.
These drawbacks could be circumvented by using the microseeding method (Fig. 1). Initially, A. calcoaceticus wild-type sGDH seeds were used to obtain crystals of all three variants as well as the two homologues. In subsequent crystallization experiments, seeds of the target proteins themselves were used. The microseeding method facilitated efficient and reproducible crystal growth of all sGDH variants tried so far (Fig. 2). An additional advantage of the microseeding method in CHES- or HEPES-containing solutions is that no buffer exchanges are required to facilitate soaking with PQQ, which has made it more straightforward to obtain crystals of PQQ-bound enzyme forms.
| || Figure 1 |
Effect of seed serial dilution (1/10 to 1/106), successively in (a) to (f), on the crystal growth of the apo-form variant A of sGDH from A. calcoaceticus. The scale bar represents 300 µm.
| || Figure 2 |
Crystals of apo forms of sGDH variants A (a), B (b) and G (c) and homologues from E. coli (d) and S. coelicolor (e, f). (e) and (f) show two different views of a S. coelicolor crystal. The scale bar represents 60 µm.
Using this method, it has also been possible to reproducibly obtain crystals with a typical maximal size of 0.1 mm and 0.3 mm for the E. coli and S. coelicolor sGDH homologues, respectively (Fig. 2). In most cases, crystals were clearly visible after 3 h and were fully grown after a couple of days. S. coelicolor control drops did not produce crystals, whereas a small number of E. coli sGDH crystals grew in some control drops after several weeks.
Data sets were collected from crystals of apo forms of each of the three sGDH variants A, B and G. The mutants crystallize in a centered orthorhombic space group rather than the monoclinic space group of wild-type crystals (Oubrie, Rozeboom, Kalk, Olsthoon et al., 1999). The asymmetric unit of the C2221 space group contains two sGDH monomers (Oubrie, Rozeboom & Dijkstra, 1999). Data-collection statistics are summarized in Table 1.
A data set was collected at 2.0 Å resolution from a single crystal of the E. coli sGDH homologue. The crystals belonged to space group P212121, with unit-cell parameters a = 59.22, b = 73.80, c = 75.80 Å. The asymmetric unit is estimated to contain one molecule of the E. coli protein, with a solvent content of 41.8%. Data-collection statistics are shown in Table 2. A data set was collected at 1.8 Å resolution from a single crystal of the apo form of the S. coelicolor sGDH homologue. The crystals belonged to space group P21, with unit-cell parameters a = 48.94, b = 40.67, c = 86.06 Å, = 93.41°. The asymmetric unit is estimated to contain one molecule of the S. coelicolor protein, with a solvent content of 47.3%. Data-collection statistics are shown in Table 2.
We are grateful to Dr Rainer Schmuck, Dr Mara Boënitz and Peter Kratzsch (Roche Diagnostics, Penzberg, Germany) for kindly providing us with samples of sGDH variants and Ann Reilly (University of East Anglia) for preparation of E. coli and S. coelicolor sGDH samples. For access to BESSY, ELETTRA and SLS, we acknowledge support from the European Community Research Infrastructure Action under the FP6 `Structuring the European Research Area' Programme (through the Integrated Infrastructure Initiative `Integrating Activity on Synchrotron and Free Electron Laser Science', contract No. RII3-CT-2004-506008).
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