Structural Biology and Crystallization Communications

 

Acta Cryst. (2006). F62, 829-834  [ doi:10.1107/S1744309106028740 ]

Trypanosoma brucei UDP-galactose-4'-epimerase in ternary complex with NAD⁺ and the substrate analogue UDP-4-deoxy-4-fluoro--D-galactose

M. S. Alphey, A. Burton, M. D. Urbaniak, G.-J. Boons, M. A. J. Ferguson and W. N. Hunter

Synopsis: The structure of recombinant T. brucei UDP-galactose-4'-epimerase cocrystallized with NAD⁺ and the substrate analogue UDP-4-deoxy-4-fluoro--D-galactose has been determined at medium resolution. Comparisons with structures of human and E. coli UDP-galactose-4'-epimerase-ligand complexes reveal that the hexose moieties are able to adopt different orientations in the active site.

PDB reference: 2cnb

Online 11 August 2006

 

Acta Cryst. (2006). F62, 835-839  [ doi:10.1107/S1744309106029125 ]

Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins

T. P. Roosild, S. Castronovo and S. Choe

Synopsis: The X-ray crystallographic analysis of anti-FLAG M2 Fab is reported and the implications of the structure on FLAG epitope binding are described as a first step in the development of a tool for the structural and biophysical study of membrane proteins.

PDB reference: 2g60

Online 18 August 2006

 

Acta Cryst. (2006). F62, 840-843  [ doi:10.1107/S1744309106029691 ]

Structure of the Y94F mutant of Escherichia coli thymidylate synthase

S. A. Roberts, D. C. Hyatt, J. E. Honts, L. Changchien, G. F. Maley, F. Maley and W. R. Montfort

Synopsis: Mutation of Tyr94 of E. coli thymidylate synthase to phenylalanine leads to a protein with k_cat reduced by a factor of 400. The Y94F structure is essentially identical to the wild-type structure, which is consistent with a catalytic role for the phenolic OH.

PDB references: 2ftn and 2fto

Online 18 August 2006

 

Acta Cryst. (2006). F62, 844-848  [ doi:10.1107/S1744309106030004 ]

Structure of the SH3 domain of human osteoclast-stimulating factor at atomic resolution

L. Chen, Y. Wang, D. Wells, D. Toh, H. Harold, J. Zhou, E. DiGiammarino and E. J. Meehan

Synopsis: The crystal structure of the SH3 domain of human osteoclast-stimulating factor has been determined and refined to the ultrahigh resolution of 1.07 Å. The structure at atomic resolution provides an accurate framework for structure-based design of its inhibitors.

PDB reference: 1zlm

Online 18 August 2006

 

Acta Cryst. (2006). F62, 849-854  [ doi:10.1107/S174430910603096X ]

Structure of Bacillus halmapalus -amylase crystallized with and without the substrate analogue acarbose and maltose

L. Lyhne-Iversen, T. J. Hobley, S. G. Kaasgaard and P. Harris

Synopsis: The structure of an uncomplexed form of -amylase from B. halmapalus is compared with a form in which maltose, glucose and a nonasaccharide derived from acarbose and maltose are bound.

PDB references: 2gjp and 2gjr

Online 26 August 2006

 

Acta Cryst. (2006). F62, 855-860  [ doi:10.1107/S1744309106031150 ]

Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8

N. Nakano, N. Okazaki, S. Satoh, K. Takio, S. Kuramitsu, A. Shinkai and S. Yokoyama

Synopsis: The crystal structure of the stand-alone RAM domain from T. thermophilus HB8 has been determined at 2.4 Å resolution. The structure revealed that five dimers are arranged to form a ring.

PDB reference: 2djw

Online 26 August 2006

 

Acta Cryst. (2006). F62, 861-864  [ doi:10.1107/S1744309106027035 ]

Expression, purification, crystallization and preliminary crystallographic analysis of BipD, a component of the Burkholderia pseudomallei type III secretion system

P. Roversi, S. Johnson, T. Field, J. E. Deane, E. E. Galyov and S. M. Lea

Synopsis: A construct consisting of residues 10-310 of mature BipD, a component of the B. pseudomallei type III secretion system, has been crystallized. Native BipD crystals and SeMet and K₂PtCl₄ derivative crystals have undergone preliminary crystallographic analysis.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 865-868  [ doi:10.1107/S1744309106027047 ]

Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of the Shigella flexneri type III secretion system

S. Johnson, P. Roversi, M. Espina, J. E. Deane, S. Birket, W. D. Picking, A. Blocker, W. L. Picking and S. M. Lea

Synopsis: IpaD, the putative needle-tip protein of the S. flexneri type III secretion system, has been crystallized in a variety of crystal forms using in-drop proteolysis. Native and selenomethionine-labelled data collection and preliminary analyses are reported.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 869-872  [ doi:10.1107/S1744309106028077 ]

Purification, identification and preliminary crystallographic studies of an allergenic protein from Lathyrus sativus

I. A. Qureshi, D. K. Sethi and D. M. Salunke

Synopsis: A 24 kDa protein was purified from the seeds of L. sativus by ammonium sulfate fractionation and ion-exchange chromatography. Crystals were obtained by the hanging-drop vapour-diffusion method.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 873-875  [ doi:10.1107/S1744309106027977 ]

Crystallization and preliminary X-ray crystallographic studies of Mycobacterium tuberculosis CRP/FNR family transcription regulator

M. Akif, Y. Akhter, S. E. Hasnain and S. C. Mande

Synopsis: The CRP/FNR family transcription factor from M. tuberculosis H37Rv has been crystallized in space group P2₁2₁2₁ in the absence of cAMP. The crystals show the presence of a dimeric molecule in the asymmetric unit.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 876-879  [ doi:10.1107/S1744309106028776 ]

Preliminary crystallographic characterization of an RNA helicase from Kunjin virus

E. Mastrangelo, M. Bollati, M. Milani, N. Brisbarre, X. Lamballerie, B. Coutard, B. Canard, A. Khromykh and M. Bolognesi

Synopsis: The C-terminal 440 amino acids of the NS3 protein from Kunjin virus (Flaviviridae) code for a helicase. The protein has been overexpressed and crystallized. Characterization of the isolated monoclinic crystal form and diffraction data (at 3.0 Å resolution) are presented, together with a preliminary molecular-replacement solution.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 880-883  [ doi:10.1107/S1744309106028764 ]

Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis

M. Barreras, M. A. Bianchet and L. Ielpi

Synopsis: Crystallization of a membrane-associated glucuronosyltransferase.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 884-886  [ doi:10.1107/S1744309106029150 ]

Crystallization and preliminary X-ray diffraction analysis of protein 14 from Sulfolobus islandicus filamentous virus (SIFV)

A. Goulet, S. Spinelli, V. Campanacci, S. Porciero, S. Blangy, R. A. Garrett, H. van Tilbeurgh, N. Leulliot, T. Basta, D. Prangishvili and C. Cambillau

Synopsis: Crystals of S. islandicus filamentous virus (SIFV) protein 14 have been grown at 293 K. Crystals belong to space group P6₂22 or P6₄22 and diffract to a resolution of 2.95 Å.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 887-890  [ doi:10.1107/S1744309106029149 ]

The purification, crystallization and preliminary structural characterization of PhzM, a phenazine-modifying methyltransferase from Pseudomonas aeruginosa

N. Gohain, L. S. Thomashow, D. V. Mavrodi and W. Blankenfeldt

Synopsis: PhzM, an S-adenosylmethionine-dependent methyltransferase enzyme that catalyzes a reaction involved in the biosynthesis of pyocyanin in P. aeruginosa, was cloned, overexpressed and crystallized. Data collection from native and selenomethionine-labelled crystals is reported.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 891-894  [ doi:10.1107/S174430910602954X ]

Crystallization and preliminary crystallographic analysis of two Streptococcus agalactiae proteins: the family II inorganic pyrophosphatase and the serine/threonine phosphatase

M. K. Rantanen, L. Lehtiö, L. Rajagopal, C. E. Rubens and A. Goldman

Synopsis: Two S. agalactiae proteins, the inorganic pyrophosphatase and the serine/threonine phosphatase, were crystallized and diffraction data were collected and processed from these crystals. The data from the two protein crystals extended to 2.80 and 2.65 Å, respectively.

Online 11 August 2006

 

Acta Cryst. (2006). F62, 895-898  [ doi:10.1107/S1744309106027825 ]

Expression, crystallization and preliminary X-ray analysis of the periplasmic stress sensory protein RseB from Escherichia coli

P. Wollmann and K. Zeth

Synopsis: The periplasmic stress protein RseB from E. coli was cloned, expressed and crystallized. Crystallographic data are presented and structure solution using the multiple isomorphous replacement approach (MIR) is in progress.

Online 18 August 2006

 

Acta Cryst. (2006). F62, 899-901  [ doi:10.1107/S174430910602968X ]

Crystallization and preliminary crystallographic analysis of a novel plant type III polyketide synthase that produces pentaketide chromone

H. Morita, S. Kondo, T. Abe, H. Noguchi, S. Sugio, I. Abe and T. Kohno

Synopsis: Pentaketide chromone synthase from A. arborescens has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 1.6 Å.

Online 18 August 2006

 

Acta Cryst. (2006). F62, 902-905  [ doi:10.1107/S1744309106030454 ]

Crystallization and preliminary X-ray diffraction study of an active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon

S. Tanaka, K. Saito, H. Chon, H. Matsumura, Y. Koga, K. Takano and S. Kanaya

Synopsis: Crystallization of and preliminary crystallographic studies on an active-site mutant of pro-Tk-subtilisin from the hyperthermophilic archaeon T. kodakaraensis were performed.

Online 18 August 2006

 

Acta Cryst. (2006). F62, 906-908  [ doi:10.1107/S1744309106030375 ]

Crystallization and preliminary crystallographic studies of the Pasteurella multocida toxin catalytic domain

M. Miyazawa, K. Kitadokoro, S. Kamitani, H. Shime and Y. Horiguchi

Synopsis: The C-terminal catalytic domain of P. multocida toxin, which is the virulence factor of the organism in P. multocida, has been expressed, purified and subsequently crystallized using the sitting-drop vapour-diffusion technique.

Online 18 August 2006

 

Acta Cryst. (2006). F62, 909-912  [ doi:10.1107/S1744309106030843 ]

Cloning, expression, purification, crystallization and initial crystallographic analysis of the preprotein translocation ATPase SecA from Thermus thermophilus

M. N. Vassylyeva, H. Mori, T. Tsukazaki, S. Yokoyama, T. H. Tahirov, K. Ito and D. G. Vassylyev

Synopsis: The SecA ATPase from T. thermophilus was cloned, expressed, purified and crystallized. Complete diffraction data sets were collected for two crystal forms at 2.8 and 3.5 Å resolution, respectively. Determination of the structure is now in progress.

Online 18 August 2006

 

Acta Cryst. (2006). F62, 913-915  [ doi:10.1107/S1744309106031125 ]

Cloning, expression, purification, crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis

M. Selvaraj, N. S. Singh, S. Roy, R. Sangeetha, U. Varshney and M. Vijayan

Synopsis: The peptidyl-tRNA hydrolase from M. tuberculosis has been crystallized in three closely related forms, two orthorhombic and one monoclinic, and X-ray diffraction data have been collected from them.

Online 26 August 2006

 

Acta Cryst. (2006). F62, 916-919  [ doi:10.1107/S1744309106031423 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of chitinase from Bacillus cereus NCTU2

C.-Y. Kuo, Y.-J. Wu, Y.-C. Hsieh, H.-H. Guan, H.-J. Tsai, Y.-H. Lin, Y.-C. Huang, M.-Y. Liu, Y.-K. Li and C.-J. Chen

Synopsis: The crystallization of B. cereus chitinase is reported.

Online 26 August 2006

 

Acta Cryst. (2006). F62, 920-922  [ doi:10.1107/S1744309106031216 ]

Crystallization of mutant forms of glutaredoxin Grx1p from yeast

K. O. Håkansson, H. Østergaard and J. R. Winther

Synopsis: Glutathionylated glutaredoxin Grx1p from yeast was crystallized, along with a yellow fluorescent protein-glutaredoxin fusion protein.

Online 26 August 2006

 

Acta Cryst. (2006). F62, 923-925  [ doi:10.1107/S1744309106031058 ]

Crystallization and preliminary X-ray diffraction analysis of rat protein tyrosine phosphatase

H. C. Matozo, A. S. Nascimento, M. A. M. Santos, R. Iuliano, A. Fusco and I. Polikarpov

Synopsis: In this study, the catalytic domain of rat protein tyrosine phosphatase was produced in Escherichia coli in soluble form and purified to homogeneity. Crystals were obtained by the hanging-drop vapour-diffusion method.

Online 26 August 2006

 

Acta Cryst. (2006). F62, 926-929  [ doi:10.1107/S1744309106029253 ]

Purification, crystallization and X-ray diffraction analysis of human synaptotagmin 1 C2A-C2B

M. Montes, K. L. Fuson, R. B. Sutton and J. J. Robert

Synopsis: Human synaptotagmin C2A-C2B has been expressed as a glutathione-S-transferase fusion protein in Escherichia coli. The purification, crystallization and preliminary X-ray analysis of this protein are reported here.

Online 26 August 2006

 

Acta Cryst. (2006). F62, 930-934  [ doi:10.1107/S1744309106030107 ]

Cloning, expression, purification, crystallization and preliminary structure determination of glucose-1-phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose-1-phosphate

D. Aragão, A. R. Marques, C. Frazão, F. J. Enguita, M. A. Carrondo, A. M. Fialho, I. Sá-Correia and E. P. Mitchell

Synopsis: Glucose-1-phosphate uridylyltransferase (UgpG) from S. elodea ATCC 31461 has been expressed, purified and crystallized. Seven crystal forms were obtained and characterized to a maximum resolution of 2.65 Å.

Online 26 August 2006

 

Acta Cryst. (2006). F62, 935-937  [ doi:10.1107/S1744309106032246 ]

Crystallization and preliminary crystallographic analysis of the tetrameric form of phosphofructokinase-2 from Escherichia coli, a member of the ribokinase family

R. Cabrera, A. Caniuguir, A. L. B. Ambrosio, V. Guixé, R. C. Garratt and J. Babul

Synopsis: The phosphofructokinase-2 enzyme from E. coli was crystallized in its tetrameric inhibited form. This is the only member of the ribokinase family known to suffer a transition from dimer to tetramer in response to the allosteric binding of MgATP.

Online 26 August 2006

 

Acta Cryst. (2006). F62, 938-943  [ doi:10.1107/S1744309106032064 ]

Crystallization and X-ray data analysis of the 10 kDa C-terminal lid subdomain from Caenorhabditis elegans Hsp70

L. Worrall and M. D. Walkinshaw

Synopsis: Crystals of the C-terminal 10 kDa lid subdomain from the C. elegans chaperone Hsp70 have been obtained that diffract X-rays to 3.5 Å and belong to space group I2₁2₁2₁. Analysis of X-ray data and initial heavy-atom phasing reveals 24 monomers in the asymmetric unit related by 432 non-crystallographic symmetry.

Online 26 August 2006