Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 1-6  [ doi:10.1107/S1744309106046823 ]

Effects of the magnesium and chloride ions and shikimate on the structure of shikimate kinase from Mycobacterium tuberculosis

M. V. B. Dias, L. M. Faím, I. B. Vasconcelos, J. S. de Oliveira, L. A. Basso, D. S. Santos and W. F. de Azevedo

Synopsis: The crystal structure of MtSK in complex with ADP shows conformational changes caused by the absence of the shikimate molecule, and the effect of chloride ions on the nucleotide binding site, and the crystal structure of MtSK in absence of magnesium shows its effect on the shikimate binding site.

PDB references: 2dfn and 2dft

Online 16 December 2006

 

Acta Cryst. (2007). F63, 7-11  [ doi:10.1107/S1744309106051773 ]

Structure of the catalytic domain of the hyperthermophilic chitinase from Pyrococcus furiosus

T. Nakamura, S. Mine, Y. Hagihara, K. Ishikawa and K. Uegaki

Synopsis: The crystal structure of the catalytic domain of a chitinase from P. furiosus is reported.

PDB reference: 2dsk

Online 16 December 2006

 

Acta Cryst. (2007). F63, 12-14  [ doi:10.1107/S1744309106051530 ]

Purification, crystallization and preliminary X-ray analysis of the BseCI DNA methyltransferase from Bacillus stearothermophilus in complex with its cognate DNA

E. G. Kapetaniou, D. Kotsifaki, M. Providaki, M. Rina, V. Bouriotis and M. Kokkinidis

Synopsis: The DNA methyltransferase M.BseCI from B. stearothermophilus was crystallized as a complex with its cognate DNA. Crystals belong to space group P6 and diffract to 2.5 Å resolution at a synchrotron source.

Online 16 December 2006

 

Acta Cryst. (2007). F63, 15-17  [ doi:10.1107/S1744309106051578 ]

Purification, crystallization and preliminary crystallographic analysis of archaeal 6-pyruvoyl tetrahydrobiopterin synthase homologue PH0634 from Pyrococcus horikoshii OT3

B. Bagautdinov, M. Sugahara and N. Kunishima

Synopsis: An archaeal 6-pyruvoyl tetrahydrobiopterin synthase homologue from P. horikoshii OT3 was overexpressed as native and selenomethionine-substituted protein, purified and crystallized. The native and selenomethionine-derivative crystals are isomorphous and diffract X-rays to 2.1 and 2.9 Å resolution, respectively.

Online 16 December 2006

 

Acta Cryst. (2007). F63, 18-20  [ doi:10.1107/S1744309106051682 ]

Expression, purification and preliminary crystallographic studies on the catalytic region of the nonreceptor tyrosine kinase Fes

I. Gnemmi, C. Scotti, D. Cappelletti, P. L. Canonico, F. Condorelli and C. Rosano

Synopsis: The catalytic domain of human Fes tyrosine kinase has been cloned, expressed, purified and crystallized.

Online 16 December 2006

 

Acta Cryst. (2007). F63, 21-23  [ doi:10.1107/S1744309106051955 ]

Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1

T. Srimathi, S. L. Robbins, R. L. Dubas, J.-H. Seo and Y. C. Park

Synopsis: The caspase-recruitment domain of the cytosolic pathogen receptor Nod1 was crystallized. X-ray diffraction data were collected to 1.9 Å resolution.

Online 16 December 2006

 

Acta Cryst. (2007). F63, 24-26  [ doi:10.1107/S1744309106052341 ]

Preliminary crystallographic analysis of avian infectious bronchitis virus main protease

J. Li, W. Shen, M. Liao and M. Bartlam

Synopsis: The avian infectious bronchitis virus main protease has been crystallized; crystals diffract to 2.7 Å resolution.

Online 16 December 2006

 

Acta Cryst. (2007). F63, 27-29  [ doi:10.1107/S1744309106052560 ]

Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus

S. P. Kanaujia, C. V. Ranjani, J. Jeyakanthan, S. Baba, L. Chen, Z.-J. Liu, B.-C. Wang, M. Nishida, A. Ebihara, A. Shinkai, S. Kuramitsu, Y. Shiro, K. Sekar and S. Yokoyama

Synopsis: The molybdenum-cofactor biosynthesis protein C from T. thermophilus has been crystallized in two different space groups, P2₁ and R32; the crystals diffracted to 1.9 and 1.75 Å resolution, respectively.

Online 16 December 2006

 

Acta Cryst. (2007). F63, 30-33  [ doi:10.1107/S1744309106052730 ]

Preliminary X-ray analysis of XC5848, a hypothetical ORFan protein with an Sm-like motif from Xanthomonas campestris

S.-K. Ruan, K.-H. Chin, H.-L. Shr, P.-C. Lyu, A.H.-J. Wang and S.-H. Chou

Synopsis: A conserved hypothetical protein Se-XC5848 from X. campestris pv. campestris has been overexpressed in E. coli, purified and crystallized. Crystals obtained from the purified recombinant protein diffracted to a resolution of 1.68 Å.

Online 16 December 2006

 

Acta Cryst. (2007). F63, 34-36  [ doi:10.1107/S1744309106053127 ]

Preliminary structural studies of the transcriptional regulator CmeR from Campylobacter jejuni

C.-C. Su, F. Shi, R. Gu, M. Li, G. McDermott, E. W. Yu and Q. Zhang

Synopsis: The transcriptional regulator CmeR from C. jejuni has been purified and crystallized and X-ray diffraction data have been collected to a resolution of 2.2 Å.

Online 16 December 2006

 

Acta Cryst. (2007). F63, 37-41  [ doi:10.1107/S174430910604509X ]

Preliminary X-ray diffraction analysis of YcdB from Escherichia coli: a novel haem-containing and Tat-secreted periplasmic protein with a potential role in iron transport

M. L. Cartron, S. A. Mitchell, M. R. Woodhall, S. C. Andrews and K. A. Watson

Synopsis: The crystallization and structure determination of the apo form of a novel haem-containing Tat substrate, YcdB from E. coli, has been solved to 2.0 Å resolution. The preliminary structure shows similarity to other haem-dependent peroxidases, despite low sequence homology.

Online 22 December 2006

 

Acta Cryst. (2007). F63, 42-45  [ doi:10.1107/S1744309106052924 ]

Preliminary time-of-flight neutron diffraction study on diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris

M.-M. Blum, A. Koglin, H. Rüterjans, B. Schoenborn, P. Langan and J.C.-H. Chen

Synopsis: Diisopropyl fluorophosphatase (DFPase) effectively hydrolyzes a number of organophosphorus nerve agents, including sarin, cyclohexylsarin, soman and tabun. Neutron diffraction data have been collected from DFPase crystals to 2.2 Å resolution in an effort to gain further insight into the mechanism of this enzyme.

Online 22 December 2006

 

Acta Cryst. (2007). F63, 46-48  [ doi:10.1107/S1744309106052870 ]

Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNA^Gly acceptor-stem microhelix

C. Förster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Fürste, C. Betzel and V. A. Erdmann

Synopsis: In order to investigate the identity elements of the E. coli tRNA^Gly/GlyRS class II system, a tRNA^Gly acceptor-stem microhelix was crystallized and a data set was collected to 2.0 Å resolution using synchrotron radiation.

Online 22 December 2006

 

Acta Cryst. (2007). F63, 49-51  [ doi:10.1107/S1744309106054145 ]

Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tails of adhesion molecules CD43 and PSGL-1

Y. Takai, K. Kitano, S. Terawaki, R. Maesaki and T. Hakoshima

Synopsis: The radixin FERM domain has been crystallized in complex with CD43 and PSGL-1 peptides. Diffraction data sets were collected from the complexes to 2.9 and 2.8 Å resolution, respectively.

Online 22 December 2006

 

Acta Cryst. (2007). F63, 52-55  [ doi:10.1107/S1744309106054522 ]

Exploration of triple-helical fragments: crystallization and preliminary X-ray diffraction of d(TGGCCTTAAGG)

K. Van Hecke, K. Uytterhoeven and L. Van Meervelt

Synopsis: The crystallization and X-ray data collection of the undecamer d(TGGCCTTAAGG) are reported.

Online 22 December 2006

 

Acta Cryst. (2007). F63, 56-58  [ doi:10.1107/S1744309106054509 ]

Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3

M. Sugahara, S. Murai, M. Sugahara and N. Kunishima

Synopsis: The putative thiamine-biosynthesis protein PH1313 from P. horikoshii OT3 was overexpressed, purified and crystallized. The crystals belong to space group P2₁2₁2₁ and diffract X-rays to 1.9 Å resolution.

Online 22 December 2006

 

Acta Cryst. (2007). F63, 59  [ doi:10.1107/S1744309106054650 ]

Crystallization and preliminary X-ray diffraction analysis of two N-terminal fragments of the DNA-cleavage domain of topoisomerase IV from Staphylococcus aureus. Corrigendum

S. B. Carr, G. Makris, S. E. V. Phillips and C. D. Thomas

Synopsis: A corrigendum to the paper by Carr et al. (2006), Acta Cryst. F62, 1164-1167.

Online 22 December 2006