Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 60-64  [ doi:10.1107/S1744309106056351 ]

Structure of cyclophilin from Leishmania donovani at 1.97 Å resolution

V. Venugopal, B. Sen, A. K. Datta and R. Banerjee

Synopsis: The crystal structure of cyclophilin from L. donovani at 1.97 Å resolution exhibits conservation of the cyclosporin binding site with respect to human cyclophilin.

PDB reference: 2haq

Online 17 January 2007

 

Acta Cryst. (2007). F63, 65-68  [ doi:10.1107/S1744309107003144 ]

Structure of rat acidic fibroblast growth factor at 1.4 Å resolution

N. Kulahin, V. Kiselyov, A. Kochoyan, O. Kristensen, J. S. Kastrup, V. Berezin, E. Bock and M. Gajhede

Synopsis: The structure of rat acidic fibroblast growth factor was determined and compared with those of human, bovine and newt origin. The rat and human structures were found to be very similar.

PDB reference: 2j3p

Online 27 January 2007

 

Acta Cryst. (2007). F63, 69-73  [ doi:10.1107/S1744309106054625 ]

Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions

H. Tanaka, T. Umehara, K. Inaka, S. Takahashi, R. Shibata, Y. Bessho, M. Sato, S. Sugiyama, E. Fusatomi, T. Terada, M. Shirouzu, S. Sano, M. Motohara, T. Kobayashi, T. Tanaka, A. Tanaka and S. Yokoyama

Synopsis: Crystallization of the transcription termination factor NusA under microgravity conditions significantly reduced the twinning content (1.0%) compared with terrestrially grown crystals (18.3%) and improved the maximum resolution from 3.0 to 2.29 Å, with similar unit-cell parameters.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 74-77  [ doi:10.1107/S1744309106054807 ]

Crystallization and preliminary X-ray analysis of cryptolepain, a novel glycosylated serine protease from Cryptolepis buchanani

M. Pande, V. K. Dubey and M. V. Jagannadham

Synopsis: Cryptolepain is a stable glycosylated novel serine protease was crystallized by hanging-drop method. Crystal data was processed up to 2.25 Å with acceptable statistics and structure determination of the enzyme is under way.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 78-83  [ doi:10.1107/S1744309106055084 ]

A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution

T. L. Yap, Y. L. Chen, T. Xu, D. Wen, S. G. Vasudevan and J. Lescar

Synopsis: Crystals of the RNA-dependent RNA polymerase catalytic domain from the dengue virus NS5 protein have been obtained using a strategy that included expression screening of naturally occurring serotype variants of the protein, the addition of divalent metal ions and crystal dehydration. These crystals diffract to 1.85 Å resolution and are thus suitable for a structure-based drug-design program.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 84-88  [ doi:10.1107/S1744309106055461 ]

Crystallization and preliminary X-ray analysis of Escherichia coli RNase HI-dsRNA complexes

L. V. Loukachevitch and M. Egli

Synopsis: To potentially gain insight into the conformational origins of substrate recognition by the enzyme from Escherichia coli, cocrystallization experiments were carried out with RNase HI-dsRNA (enzyme-inhibitor) complexes. Crystals were obtained of two complexes containing 9-mer and 10-mer RNA duplexes that diffracted X-rays to 3.5 and 4 Å resolution, respectively.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 89-92  [ doi:10.1107/S1744309106055850 ]

Crystallization and preliminary X-ray analysis of MotY, a stator component of the Vibrio alginolyticus polar flagellar motor

A. Shinohara, M. Sakuma, T. Yakushi, S. Kojima, K. Namba, M. Homma and K. Imada

Synopsis: Crystals of MotY, a stator protein of the V. alginolyticus polar flagellar motor, have been produced and characterized by X-ray diffraction.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 93-95  [ doi:10.1107/S1744309106055849 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* sialic acid-binding domain of porcine rotavirus strain OSU

Y.-D. Zhang, H. Li, H. Liu and Y.-F. Pan

Synopsis: Porcine rotavirus strain OSU VP8* domain has been expressed, purified and crystallized. X-ray diffraction data from different crystal forms of the VP8* domain have been collected to 2.65 and 2.2 Å resolution, respectively.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 96-98  [ doi:10.1107/S1744309106055837 ]

Purification, crystallization and preliminary X-ray analysis of the regulatory subunit of aspartate kinase from Thermus thermophilus

A. Yoshida, T. Tomita, T. Kuzuyama and M. Nishiyama

Synopsis: To elucidate the mechanism of regulation of aspartate kinase, the regulatory subunit (the subunit of T. thermophilus aspartate kinase) was crystallized in the presence of the inhibitor threonine.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 99-102  [ doi:10.1107/S1744309106056429 ]

Crystallization and preliminary crystallographic analysis of human Atg4B-LC3 complex

K. Satoo, N. N. Suzuki, Y. Fujioka, N. Mizushima, Y. Ohsumi and F. Inagaki

Synopsis: Human Atg4B and LC3 were expressed, purified and crystallized as a complex. Diffraction data were collected to a resolution of 1.9 Å.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 103-105  [ doi:10.1107/S1744309106056521 ]

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus

S. P. Kanaujia, C. V. Ranjani, J. Jeyakanthan, S. Baba, C. Kuroishi, A. Ebihara, A. Shinkai, S. Kuramitsu, Y. Shiro, K. Sekar and S. Yokoyama

Synopsis: DHNA synthetase from G. kaustophilus has been cloned, expressed, purified and crystallized.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 106-109  [ doi:10.1107/S1744309107000103 ]

Crystallization and X-ray diffraction analysis of the -ketoacyl-acyl carrier protein reductase FabG from Aquifex aeolicus VF5

Q. Mao, W. L. Duax and T. C. Umland

Synopsis: FabG from A. aeolicus, a putative component of fatty-acid synthase II, has been overexpressed, purified and crystallized. Diffraction data have been collected to 1.8 Å resolution.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 110-113  [ doi:10.1107/S1744309107000474 ]

Crystallization and X-ray diffraction analysis of the DNA-remodelling protein DnaD from Bacillus subtilis

S. Schneider, M. J. V. M. Carneiro, C. Ioannou, P. Soultanas and M. Paoli

Synopsis: Crystallization and preliminary X-ray diffraction analysis of the two domains of DnaD from B. subtilis is reported.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 114-116  [ doi:10.1107/S1744309107000681 ]

Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae

W. Liu, Y.-Y. Zhou, M.-K. Teng and C.-Z. Zhou

Synopsis: Heat-shock protein Hsp33 from S. cerevisiae was crystallized and diffraction data were collected to 2.7 Å resolution.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 117-119  [ doi:10.1107/S1744309107000863 ]

Crystallization and preliminary X-ray diffraction analysis of -amino acid:pyruvate transaminase from Chromobacterium violaceum

C. Sayer, M. N. Isupov and J. A. Littlechild

Synopsis: An -amino acid:pyruvate transaminase from C. violaceum has been purified and crystallized in two crystal forms. The structure has been solved using molecular replacement.

Online 17 January 2007

 

Acta Cryst. (2007). F63, 120-122  [ doi:10.1107/S1744309107001078 ]

Crystallization and preliminary crystallographic analysis of DtsR1, a carboxyltransferase subunit of acetyl-CoA carboxylase from Corynebacterium glutamicum

M. Yamada, R. Natsume, T. Nakamatsu, S. Horinouchi, H. Kawasaki and T. Senda

Synopsis: DtsR1, a carboxyltransferase subunit of acetyl-CoA carboxylase from C. glutamicum, was crystallized and phases were obtained by molecular replacement.

Online 27 January 2007

 

Acta Cryst. (2007). F63, 123-125  [ doi:10.1107/S1744309107001169 ]

Purification, crystallization and preliminary X-ray diffraction studies of D-tagatose 3-epimerase from Pseudomonas cichorii

H. Yoshida, M. Yamada, T. Nishitani, G. Takada, K. Izumori and S. Kamitori

Synopsis: Recombinant D-tagatose 3-epimerase from P. cichorii was purified and crystallized. Diffraction data were collected to 2.5 Å resolution.

Online 27 January 2007

 

Acta Cryst. (2007). F63, 126-129  [ doi:10.1107/S1744309107001388 ]

Crystallization and preliminary X-ray diffraction study of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis

R. Katsumi, Y. Koga, D.-J. You, H. Matsumura, K. Takano and S. Kanaya

Synopsis: Glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis was crystallized and preliminary crystallographic studies of the crystals were performed.

Online 27 January 2007

 

Acta Cryst. (2007). F63, 130-134  [ doi:10.1107/S1744309107002564 ]

Purification, partial characterization, crystallization and preliminary X-ray diffraction of a novel cardiotoxin-like basic protein from Naja naja atra (South Anhui) venom

H. Rong, Y. Li, X. Lou, X. Zhang, Y. Gao, M. Teng and L. Niu

Synopsis: A novel cardiotoxin-like basic protein from Naja naja atra was crystallized and diffraction data were collected to 2.35 Å resolution.

Online 27 January 2007

 

Acta Cryst. (2007). F63, 135-138  [ doi:10.1107/S1744309107002904 ]

The use of in situ proteolysis in the crystallization of murine CstF-77

Y. Bai, T. C. Auperin and L. Tong

Synopsis: In situ proteolysis with fungal protease or subtilisin is crucial for the crystallization of murine CstF-77.

Online 27 January 2007

 

Acta Cryst. (2007). F63, 139-141  [ doi:10.1107/S1744309107003363 ]

Crystallization and preliminary X-ray crystallographic analysis of the receptor-uncoupled mutant of G_i1

T. Morikawa, A. Muroya, Y. Nakajima, T. Tanaka, K. Hirai, S. Sugio, K. Wakamatsu and T. Kohno

Synopsis: The K349P mutant of G_i1, which is unable to couple to G-protein-coupled receptors, has been crystallized and analyzed. The same crystallization conditions were applicable irrespective of the identity of the bound nucleotide or of the presence of the mutation.

Online 27 January 2007

 

Acta Cryst. (2007). F63, 142-144  [ doi:10.1107/S174430910700334X ]

Crystallization and preliminary X-ray crystallographic studies of Pz peptidase A from Geobacillus collagenovorans MO-1

A. Kawasaki, H. Nakano, Y. Tsujimoto, H. Matsui, T. Shimizu, T. Nakatsu, H. Kato and K. Watanabe

Synopsis: Pz peptidase A has been cocrystallized with a phosphine peptide inhibitor (PPI) that selectively inhibits thimet oligopeptidase and neurolysin.

Online 27 January 2007

 

Acta Cryst. (2007). F63, 145-149  [ doi:10.1107/S1744309107004939 ]

Notes for authors 2007

Online 31 January 2007