). The linear increase in absorbance at 230 nm arising from the formation of L-DHO (![[epsilon]](/logos/entities/epsiv_rmgif.gif)
230 = 1.17 mM-1 cm-1) was measured and the initial reaction rates were calculated.
1The relative enzyme activity was measured by direct spectrophotometric assay at 230 nm (Sander et al., 1965). The linear increase in absorbance at 230 nm arising from the formation of L-DHO (230 = 1.17 mM-1 cm-1) was measured and the initial reaction rates were calculated.