Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 150-153  [ doi:10.1107/S174430910700379X ]

A high-resolution structure of ligand-free human glutamate carboxypeptidase II

C. Barinka, J. Starkova, J. Konvalinka and J. Lubkowski

Synopsis: A crystal structure of ligand-free human glutamate carboxypeptidase II refined to 1.65 Å resolution is reported. The structure provides insight into the active-site of the enzyme in its unliganded state.

PDB reference: 2oot

Online 13 February 2007

 

Acta Cryst. (2007). F63, 154-161  [ doi:10.1107/S1744309107004009 ]

Structure of the T109S mutant of Escherichia coli dihydroorotase complexed with the inhibitor 5-fluoroorotate: catalytic activity is reflected by the crystal form

M. Lee, M. J. Maher and J. M. Guss

Synopsis: A single-point mutant (T109S) of E. coli dihydroorotase initially crystallizes so that the two monomers of the dimer are related by a crystallographic twofold axis. In the presence of substrate, conversion to the previously observed asymmetric dimer with substrate bound in one subunit and product in the other is observed.

PDB reference: 2e25

Online 13 February 2007

 

Acta Cryst. (2007). F63, 162-167  [ doi:10.1107/S1744309107005271 ]

Monellin (MNEI) at 1.15 Å resolution

J. R. Hobbs, S. D. Munger and G. L. Conn

Synopsis: The crystal structure of the sweet protein MNEI at 1.15 Å resolution reveals networks of alternate conformations and stably bound negative ions.

PDB reference: 2o9u

Online 13 February 2007

 

Acta Cryst. (2007). F63, 168-172  [ doi:10.1107/S1744309107007221 ]

Structure of 5-formyltetrahydrofolate cyclo-ligase from Bacillus anthracis (BA4489)

C. Meier, L. G. Carter, G. Winter, R. J. Owens, D. I. Stuart and R. M. Esnouf

Synopsis: The structure of 5-formyltetrahydrofolate cyclo-ligase from B. anthracis determined by X-ray crystallography at a resolution of 1.6 Å is described.

PDB reference: 2jcb

Online 23 February 2007

 

Acta Cryst. (2007). F63, 173-177  [ doi:10.1107/S1744309107006070 ]

Structure of a UPF0150-family protein from Thermus thermophilus HB8

N. Okazaki, M. Kumei, M. Manzoku, S. Kuramitsu, M. Shirouzu, A. Shinkai and S. Yokoyama

Synopsis: The crystal structure of the hypothetical protein TTHA0281 from T. thermophilus HB8 has been determined at 1.9 Å resolution. The TTHA0281 protein forms a homotetramer in which each monomer adopts an ---- fold.

PDB reference: 2dsy

Online 23 February 2007

 

Acta Cryst. (2007). F63, 178-182  [ doi:10.1107/S1744309107007580 ]

The structure of Plasmodium vivax phosphatidylethanolamine-binding protein suggests a functional motif containing a left-handed helix

T. Arakaki, H. Neely, E. Boni, N. Mueller, F. S. Buckner, W. C. Van Voorhis, A. Lauricella, G. DeTitta, J. Luft, W. G. J. Hol and E. A. Merritt

Synopsis: The crystal structure of a phosphatidylethanolamine-binding protein from P. vivax, a homolog of Raf-kinase inhibitor protein (RKIP), has been solved to a resolution of 1.3 Å. The inferred interaction surface near the anion-binding site is found to include a distinctive left-handed -helix.

PDB reference: 2gzq

Online 23 February 2007

 

Acta Cryst. (2007). F63, 183-186  [ doi:10.1107/S174430910700437X ]

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis

G. N. Hatzopoulos and J. Mueller-Dieckmann

Synopsis: Initiation factor 1 from M. tuberculosis was cloned, expressed, purified and crystallized. A complete data set has been collected to 1.47 Å resolution.

Online 10 February 2007

 

Acta Cryst. (2007). F63, 187-189  [ doi:10.1107/S1744309107004575 ]

Extracellular overproduction and preliminary crystallographic analysis of a family I.3 lipase

C. Angkawidjaja, D.-J. You, H. Matsumura, Y. Koga, K. Takano and S. Kanaya

Synopsis: A family I.3 lipase from Pseudomonas sp. MIS38 was secreted from Escherichia coli cells to the external medium, purified and crystallized and preliminary crystallographic studies were performed.

Online 10 February 2007

 

Acta Cryst. (2007). F63, 190-192  [ doi:10.1107/S1744309107004848 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the non-ATPase subunit Nas6 in complex with the ATPase subunit Rpt3 of the 26S proteasome from Saccharomyces cerevisiae

Y. Nakamura, T. Umehara, A. Tanaka, M. Horikoshi, B. Padmanabhan and S. Yokoyama

Synopsis: The complex of the non-ATPase subunit Nas6 with the C-terminal domain of the ATPase subunit Rpt3 of the 26S proteasome from S. cerevisiae was co-expressed in E. coli and purified to homogeneity. The crystals obtained from the protein complex diffracted to a resolution of 2.2 Å.

Online 10 February 2007

 

Acta Cryst. (2007). F63, 193-195  [ doi:10.1107/S1744309107005283 ]

Purification and crystallization of Kokobera virus helicase

L. De Colibus, S. Speroni, B. Coutard, N. L. Forrester, E. Gould, B. Canard and A. Mattevi

Synopsis: Kokobera virus is a mosquito-borne flavivirus belonging, like West Nile virus, to the Japanese encephalitis virus serocomplex. Crystals of the Kokobera virus helicase domain were obtained by the hanging-drop vapour-diffusion method and exhibit a diffraction limit of 2.3 Å.

Online 10 February 2007

 

Acta Cryst. (2007). F63, 196-199  [ doi:10.1107/S174430910700509X ]

Preparation and preliminary X-ray diffraction analysis of crystals of bacterial flagellar sigma factor ²⁸ in complex with the ²⁸-binding region of its antisigma factor, FlgM

K. Okada, H. Ichihara, H. Takahashi, N. Fujita, A. Ishihama and T. Hakoshima

Synopsis: A complex of E. coli flagellar and chemotaxis-specific sigma factor ²⁸ bound to the ²⁸-binding region of its antisigma factor FlgM was crystallized. Diffraction data were collected to a resolution of 2.7 Å.

Online 10 February 2007

 

Acta Cryst. (2007). F63, 200-203  [ doi:10.1107/S1744309107005441 ]

Crystallization of Saccharomyces cerevisiae aminopeptidase 1, the major cargo protein of the Cvt pathway

W. Adachi, N. N. Suzuki, Y. Fujioka, K. Suzuki, Y. Ohsumi and F. Inagaki

Synopsis: Aminopeptidase 1, a cargo protein in the cytosol-to-vacuole targeting (Cvt) pathway, was expressed, purified and crystallized in two crystal forms.

Online 10 February 2007

 

Acta Cryst. (2007). F63, 204-208  [ doi:10.1107/S1744309107004903 ]

Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1

N. Dimasi, D. Flot, F. Dupeux and J. A. Márquez

Synopsis: IREM-1 (immune receptor expressed on myeloid cells 1) is an inhibitory receptor of the immunoglobulin superfamily involved in the functional regulation of myeloid cells. It contains an extracellular region comprising one IgV-like domain. The extracellular IgV-like domain, believed to be involved in target-cell recognition, has been expressed in E. coli, folded in vitro and crystallized.

Online 13 February 2007

 

Acta Cryst. (2007). F63, 209-213  [ doi:10.1107/S1744309107004599 ]

The use of Co²⁺ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein

G. Guncar, C.-I.A. Wang, J. K. Forwood, T. Teh, A.-M. Catanzariti, J. G. Ellis, P. N. Dodds and B. Kobe

Synopsis: It is demonstrated that anomalous diffraction based on the signal from a cobalt ion measured on a conventional monochromatic X-ray source can be used to determine the structure of a protein with a novel fold (M. lini avirulence protein AvrL567-A). The approach could be applicable to many metal-binding proteins, particularly when synchrotron radiation is not readily available.

PDB reference: 2opc

Online 23 February 2007

 

Acta Cryst. (2007). F63, 214-216  [ doi:10.1107/S1744309107005830 ]

Crystallization, diffraction data collection and preliminary crystallographic analysis of hexagonal crystals of Pseudomonas aeruginosa amidase

J. Andrade, A. Karmali, M. A. Carrondo and C. Frazão

Synopsis: Crystals of aliphatic amidase (acylamide amidohydrolase; EC 3.5.1.4) from P. aeruginosa were obtained in space group P6₃22 and diffracted to 1.25 Å resolution.

Online 23 February 2007

 

Acta Cryst. (2007). F63, 217-223  [ doi:10.1107/S1744309107005957 ]

Crystallization and molecular-replacement solution of a diagnostic fluorescent dye in complex with a specific Fab fragment

R. C. Hillig, S. Baesler, S. Urlinger, Y. Stark, S. Bauer, V. Badock, M. Huber, I. Bahr, M. Schirner and K. Licha

Synopsis: The complex formed by a Fab antibody fragment and the indotricarbocyanine dye tetrasulfocyanine (TSC) has been crystallized in three different crystal forms. For two of these, data sets were collected, molecular-replacement solutions were identified and refinement has been started.

Online 23 February 2007

 

Acta Cryst. (2007). F63, 224-228  [ doi:10.1107/S1744309107006264 ]

Crystallization and preliminary X-ray crystallographic study of alanyl-tRNA synthetase from the archaeon Archaeoglobus fulgidus

R. Fukunaga and S. Yokoyama

Synopsis: The N- and C-terminal fragments of alanyl-tRNA synthetase from the archaeon A. fulgidus have been crystallized.

Online 23 February 2007

 

Acta Cryst. (2007). F63, 229-232  [ doi:10.1107/S1744309107007889 ]

Crystallization and preliminary X-ray crystallographic studies of the oligomeric death-domain complex between PIDD and RAIDD

H. H. Park and H. Wu

Synopsis: The PIDD DD-RAIDD DD complex has been crystallized. The crystals are hexagonal and belong to space group P6₅, with unit-cell parameters a = b = 138.4, c = 207.6 Å. The crystals were obtained at room temperature; a native crystal diffracted to 3.2 Å resolution and a Hg-derivatized crystal to 4.0 Å resolution.

Online 23 February 2007

 

Acta Cryst. (2007). F63, 233-235  [ doi:10.1107/S1744309107007944 ]

Crystallization and preliminary X-ray analysis of AbsC, a novel regulator of antibiotic production in Streptomyces coelicolor

C. E. M. Stevenson, H. Kock, S. Mootien, S. C. Davies, M. J. Bibb and D. M. Lawson

Synopsis: A novel regulator of antibiotic production in S. coelicolor, AbsC, has been crystallized in space group P2₁2₁2₁. X-ray data to 2.25 Å resolution were collected on station PX 14.1 at Daresbury.

Online 23 February 2007

 

Acta Cryst. (2007). F63, 236-238  [ doi:10.1107/S1744309107008287 ]

Crystallization and preliminary X-ray analysis of the O-methyltransferase NovP from the novobiocin-biosynthetic cluster of Streptomyces spheroides

C. E. M. Stevenson, C. L. Freel Meyers, C. T. Walsh and D. M. Lawson

Synopsis: Monoclinic crystals of NovP, an O-methyltransferase from S. spheroides, were obtained and native X-ray data to 1.4 Å resolution were recorded.

Online 28 February 2007

 

Acta Cryst. (2007). F63, 239-241  [ doi:10.1107/S1744309107008500 ]

Purification, crystallization and preliminary crystallographic analysis of a GTP-binding protein from the hyperthermophilic archaeon Sulfolobus solfataricus

H. Wu, L. Sun, S. J. J. Brouns, S. Fu, J. Akerboom, X. Li and J. van der Oost

Synopsis: A GTP-binding protein from the hyperthermophilic archaeon Sulfolobus solfataricus has been crystallized. Combined with biochemical analyses, it is expected that the structure of this protein will give insight in the function of a relatively unknown subfamily of the GTPase superfamily.

Online 28 February 2007

 

Acta Cryst. (2007). F63, 242-245  [ doi:10.1107/S1744309107008524 ]

Crystallization and preliminary X-ray diffraction studies of trypsin-like proteases from the gastric fluid of the marine crab Cancer pagurus

J.-H. Hehemann, L. Redecke, M. Perbandt, R. Saborowski and C. Betzel

Synopsis: Two trypsins from the gastric fluid of the marine crab C. pagurus were purified and crystallized and X-ray data were collected to 0.97 and 3.2 Å resolution.

Online 28 February 2007

 

Acta Cryst. (2007). F63, 246-248  [ doi:10.1107/S1744309107008469 ]

Crystallization and preliminary X-ray crystallographic analysis of enoyl-ACP reductase III (FabL) from Bacillus subtilis

K.-H. Kim, J. K. Park, B. H. Ha, J. H. Moon and E. E. Kim

Synopsis: Enoyl-ACP reductase III (FabL) from B. subtilis has been overexpressed, purified and crystallized. The crystal belongs to space group P622, with unit-cell parameters a = b = 139.56, c = 62.75 Å, = = 90, = 120°, and data were collected to 2.5 Å resolution using synchrotron radiation.

Online 28 February 2007

 

Acta Cryst. (2007). F63, 249-251  [ doi:10.1107/S1744309107008834 ]

Crystallization and preliminary X-ray characterization of 1,3-propanediol dehydrogenase from the human pathogen Klebsiella pneumoniae

D. Marçal, A. T. Rego, M. J. Fogg, K. S. Wilson, M. A. Carrondo and F. J. Enguita

Synopsis: 1,3-Propanediol dehydrogenase from K. pneumoniae has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.7 Å resolution.

Online 28 February 2007

 

Acta Cryst. (2007). F63, 252  [ doi:10.1107/S1744309107009098 ]

Preliminary characterization of (nucleoside-2'-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses. Corrigendum

E. Mastrangelo, M. Bollati, M. Milani, X. De Lamballerie, N. Brisbarre, K. Dalle, V. Lantez, M.-P. Egloff, B. Coutard, B. Canard, E. Gould, N. Forrester and M. Bolognesi

Synopsis: A corrigendum to the paper by Mastrangelo et al. (2006), Acta Cryst. F62, 768-770.

Online 28 February 2007