Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 361-368  [ doi:10.1107/S1744309107019185 ]

Oligomerization of BenM, a LysR-type transcriptional regulator: structural basis for the aggregation of proteins in this family

O. C. Ezezika, S. Haddad, E. L. Neidle and C. Momany

Synopsis: A general tetramerization and oligomerization scheme is proposed for BenM and other LysR-type transcriptional regulators based on two newly characterized structures of the effector-binding domain of BenM. An analysis of these structures in the light of other LysR-type structures may explain the general solubility problems associated with this protein family.

PDB references: 2f97 and 2f8d

Online 28 April 2007

 

Acta Cryst. (2007). F63, 369-374  [ doi:10.1107/S1744309107018945 ]

Structure of O67745_AQUAE, a hypothetical protein from Aquifex aeolicus

V. Oganesyan, P. D. Adams, J. Jancarik, R. Kim and S.-H. Kim

Synopsis: Using single-wavelength anomalous dispersion data obtained from a gold-derivatized crystal, the X-ray crystal structure of the protein 067745_AQUAE from the prokaryotic organism Aquifex aeolicus has been determined to a resolution of 2.0 Å.

PDB reference: 2hek

Online 28 April 2007

 

Acta Cryst. (2007). F63, 375-377  [ doi:10.1107/S1744309107006069 ]

Crystallization of human nicotinamide phosphoribosyltransferase

R. Takahashi, S. Nakamura, T. Yoshida, Y. Kobayashi and T. Ohkubo

Synopsis: Human nicotinamide phosphoribosyltransferase has been crystallized using microseeding methods and X-ray diffraction data have been collected at 2.0 Å resolution.

Online 6 April 2007

 

Acta Cryst. (2007). F63, 378-381  [ doi:10.1107/S1744309107013760 ]

Crystallization and diffraction data of 1H-3-hydroxy-4-oxoquinoline 2,4-dioxygenase: a cofactor-free oxygenase of the /-hydrolase family

R. Qi, S. Fetzner and A. J. Oakley

Synopsis: Preliminary crystallographic data for 1H-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from P. putida 33/1 are reported.

Online 6 April 2007

 

Acta Cryst. (2007). F63, 382-385  [ doi:10.1107/S174430910701353X ]

Crystallization and preliminary X-ray analysis of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter nitroguajacolicus Rü61a: a cofactor-devoid dioxygenase of the /-hydrolase-fold superfamily

R. A. Steiner, U. Frerichs-Deeken and S. Fetzner

Synopsis: Preliminary crystallographic data are reported for 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) from A. nitroguajacolicus Rü61a.

Online 6 April 2007

 

Acta Cryst. (2007). F63, 386-392  [ doi:10.1107/S1744309107013693 ]

Humidity control as a strategy for lattice optimization applied to crystals of HLA-A*1101 complexed with variant peptides from dengue virus

P. Chotiyarnwong, G. B. Stewart-Jones, M. Tarry, W. Dejnirattisai, C. Siebold, M. Koch, D. I. Stuart, K. Harlos, P. Malasit, G. Screaton, J. Mongkolsapaya and E. Y. Jones

Synopsis: Crystals of an MHC class I molecule bound to naturally occurring peptide variants from the dengue virus NS3 protein contained high levels of solvent and required optimization of cryoprotectant and dehydration protocols for each complex to yield well ordered diffraction, a process facilitated by the use of a free-mounting system.

Online 6 April 2007

 

Acta Cryst. (2007). F63, 393-395  [ doi:10.1107/S1744309107015102 ]

Preliminary crystallographic analysis of L-2-keto-3-deoxyarabonate dehydratase, an enzyme involved in an alternative bacterial pathway of L-arabinose metabolism

N. Shimada, B. Mikami, S. Watanabe and K. Makino

Synopsis: L-2-Keto-3-deoxyarabonate dehydratase was overexpressed, purified and crystallized at 291 K using the hanging-drop vapour-diffusion method.

Online 6 April 2007

 

Acta Cryst. (2007). F63, 396-398  [ doi:10.1107/S1744309107015345 ]

Crystallization and X-ray analysis of the salmon-egg lectin SEL24K

K. Murata, A. J. Fisher and J. L. Hedrick

Synopsis: The 24 kDa egg lectin of Chinook salmon (Oncorhynchus tshawytscha) was purified by affinity chromatography from salmon eggs and crystallized by the hanging-drop vapor-diffusion method using 15/4 EO/OH (pentaerythritol ethoxylate) as a precipitant.

Online 6 April 2007

 

Acta Cryst. (2007). F63, 399-402  [ doi:10.1107/S1744309107017113 ]

Cloning, expression, purification, crystallization and preliminary X-ray analysis of Thermus aquaticus succinyl-CoA synthetase

M. A. Joyce, E. R. Brownie, K. Hayakawa and M. E. Fraser

Synopsis: Attempts to crystallize succinyl-CoA synthetase from the thermophile T. aquaticus were thwarted by proteolysis of the -subunit and preferential crystallization of a truncated form. Crystals of the full-length enzyme were grown after the purification protocol was modified to include frequent additions of protease inhibitors.

Online 14 April 2007

 

Acta Cryst. (2007). F63, 403-405  [ doi:10.1107/S1744309107015357 ]

Crystallization and preliminary X-ray diffraction data of the rat histone H1⁰ globular domain

T. Matsui, S. Nishimura, K. Kijima, Y. Kawasaki, F. Tashiro and S. Miura

Synopsis: Crystals of the rat histone H1⁰ globular domain have been grown in the trigonal space group P3₁21 and diffracted to a resolution of 1.98 Å. Molecular-replacement solutions were identified and refinement has been started.

Online 14 April 2007

 

Acta Cryst. (2007). F63, 406-408  [ doi:10.1107/S1744309107015278 ]

Crystallization and preliminary X-ray analysis of a RecB-family nuclease from the archaeon Pyrococcus abyssi

B. Ren, J. Kuhn, L. Meslet-Cladiere, H. Myllykallio and R. Ladenstein

Synopsis: A RecB-like nuclease from the archaeon Pyrococcus abyssi was expressed, purified and crystallized. The crystals belong to the orthorhombic space group C222₁ with a = 81.5, b = 159.8, c = 100.8 Å, and a native data set was collected to 2.65 Å resolution.

Online 14 April 2007

 

Acta Cryst. (2007). F63, 409-411  [ doi:10.1107/S1744309107016004 ]

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana

M. Bajaj and H. Moriyama

Synopsis: The first crystallization of deoxyuridine triphosphate nucleotidohydrolase from plant, Arabidopsis thaliana, has been performed. An additive, taurine, was effective in producing the single crystal.

Online 14 April 2007

 

Acta Cryst. (2007). F63, 412-414  [ doi:10.1107/S1744309107016685 ]

Purification, crystallization and preliminary X-ray diffraction studies of a putative UDP-N-acetyl-D-mannosamine dehydrogenase from Pyrococcus horikoshii OT3

N. K. Lokanath, K. J. Pampa, T. Kamiya and N. Kunishima

Synopsis: A putative UDP-N-acetyl-D-mannosamine dehydrogenase from P. horikoshii OT3 has been crystallized in space group P2₁, with unit-cell parameters a = 80.28, b = 69.24, c = 83.10 Å, = 114.4°. X-ray diffraction data have been collected to 1.80 Å resolution.

Online 14 April 2007

 

Acta Cryst. (2007). F63, 415-418  [ doi:10.1107/S1744309107016880 ]

Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK

C.-L. Keiski, P. Yip, H. Robinson, L. L. Burrows and P. L. Howell

Synopsis: The cloning, expression, purification and crystallization of wild-type AlgK from P. fluorescens is described. The crystals diffract to 2.5 Å resolution.

Online 14 April 2007

 

Acta Cryst. (2007). F63, 419-421  [ doi:10.1107/S174430910701723X ]

Crystallization and preliminary X-ray analysis of the mRNA-binding domain of elongation factor SelB from Escherichia coli in complex with RNA

N. Soler, D. Fourmy and S. Yoshizawa

Synopsis: The mRNA-binding domain of E. coli selenocysteine-specific elongation factor SelB (residues 478-614; SelB-WH3/4) was overproduced in E. coli and its cognate mRNA ligand, 23 nucleotides of the SECIS RNA hairpin, was prepared by in vitro transcription. The purified SelB-WH3/4-SECIS RNA complex crystallized in space group C2 and diffracted to 2.3 Å.

Online 20 April 2007

 

Acta Cryst. (2007). F63, 422-425  [ doi:10.1107/S1744309107017411 ]

Crystallization and preliminary X-ray diffraction studies of a ferredoxin reductase from Rhodopseudomonas palustris CGA009

Y. Peng, F. Xu, S. G. Bell, L.-L. Wong and Z. Rao

Synopsis: Palustrisredoxin reductase (RPA3782, PuR), a flavin-dependent ferredoxin reductase, is an essential component of the Class I cytochrome P450 systems in Rhodopseudomonas palustris CGA009. Crystals of PuR that diffract to 2.2 Å resolution have been obtained.

Online 20 April 2007

 

Acta Cryst. (2007). F63, 426-429  [ doi:10.1107/S1744309107017988 ]

Crystallization of the avian reovirus double-stranded RNA-binding and core protein A

X. L. Hermo-Parrado, P. Guardado-Calvo, A. L. Llamas-Saiz, G. C. Fox, L. Vazquez-Iglesias, J. Martínez-Costas, J. Benavente and M. J. van Raaij

Synopsis: The avian reovirus double-stranded RNA-binding and core protein A has been crystallized in space group P1, with unit-cell parameters a = 103.2, b = 129.9, c = 144.0 Å, = 93.8, = 105.1, = 98.2°. A complete data set has been collected to 2.3 Å resolution and analyzed.

Online 20 April 2007

 

Acta Cryst. (2007). F63, 430-433  [ doi:10.1107/S1744309107018271 ]

Crystallization and preliminary X-ray diffraction studies of hexokinase KlHxk1 from Kluyveromyces lactis

E. B. Kuettner, T. M. Kriegel, A. Keim, M. Naumann and N. Sträter

Synopsis: Crystals of the enzyme hexokinase 1 from the yeast K. lactis (KlHxk1) suitable for X-ray analysis were obtained in various space groups.

Online 20 April 2007

 

Acta Cryst. (2007). F63, 434-437  [ doi:10.1107/S1744309107019045 ]

Crystallization of a Mos1 transposase-inverted-repeat DNA complex: biochemical and preliminary crystallographic analyses

J. M. Richardson, D. J. Finnegan and M. D. Walkinshaw

Synopsis: Full-length Mos1 transposase has been crystallized in a complex with inverted-repeat DNA. Crystals diffract to 3.25 Å resolution and display noncrystallographic twofold symmetry.

Online 20 April 2007

 

Acta Cryst. (2007). F63, 438-442  [ doi:10.1107/S1744309107018787 ]

The crystallization of apo-form UMP kinase from Xanthomonas campestris is significantly improved in a strong magnetic field

J.-L. Tu, K.-H. Chin, A.H.-J. Wang and S.-H. Chou

Synopsis: A bacterial UMP kinase from the plant pathogen X. campestris pathovar campestris has been overexpressed in E. coli, purified and crystallized in a strong magnetic field. The crystals diffracted to 2.35 Å.

Online 20 April 2007

 

Acta Cryst. (2007). F63, 443-445  [ doi:10.1107/S1744309107017472 ]

Crystallization and preliminary X-ray analysis of Atg10

M. Yamaguti, N. N. Suzuki, Y. Fujioka, Y. Ohsumi and F. Inagaki

Synopsis: S. cerevisiae Atg10, an E2-like enzyme that mediates the conjugation reaction between Atg12 and Atg5, was crystallized and diffracted to 2.3 Å resolution.

Online 21 April 2007

 

Acta Cryst. (2007). F63, 446-448  [ doi:10.1107/S1744309107014819 ]

Preliminary X-ray crystallographic study of glucose dehydrogenase from Thermus thermophilus HB8

S. P. Kanaujia, C. V. Ranjani, J. Jeyakanthan, M. Nishida, Y. Kitamura, S. Baba, A. Ebihara, N. Shimizu, N. Nakagawa, A. Shinkai, M. Yamamoto, S. Kuramitsu, Y. Shiro, K. Sekar and S. Yokoyama

Synopsis: The glucose dehydrogenase (GDH) protein from T. thermophilus HB8 was cloned, expressed, purified and crystallized. GDH crystals belong to space group P2₁ and diffract to 1.9 Å resolution.

Online 28 April 2007

 

Acta Cryst. (2007). F63, 449-451  [ doi:10.1107/S1744309107020180 ]

Expression, purification and preliminary crystallographic characterization of FlhF from Bacillus subtilis

G. Bange, G. Petzold, K. Wild and I. Sinning

Synopsis: Preliminary crystallographic data are reported for the third SRP GTPase FlhF from Bacillus subtilis.

Online 28 April 2007

 

Acta Cryst. (2007). F63, 452-456  [ doi:10.1107/S1744309107019562 ]

Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase

J. Seetharamappa, M. Oke, H. Liu, S. A. McMahon, K. A. Johnson, L. Carter, M. Dorward, M. Zawadzki, I. M. Overton, C. A. J. van Niekirk, S. Graham, C. H. Botting, G. L. Taylor, M. F. White, G. J. Barton, P. J. Coote and J. H. Naismith

Synopsis: As part of work on S. aureus, the crystallization of Sar2028, a protein that is upregulated in MRSA, is reported.

Online 28 April 2007