Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 457-461  [ doi:10.1107/S1744309107020295 ]

Purification, crystallization and structure determination of native GroEL from Escherichia coli lacking bound potassium ions

P. D. Kiser, D. T. Lodowski and K. Palczewski

Synopsis: A 3.02 Å crystal structure of native GroEL from E. coli is presented.

PDB reference: 2nwc

Online 5 May 2007

 

Acta Cryst. (2007). F63, 462-465  [ doi:10.1107/S1744309107021422 ]

New insights into the binding mode of coenzymes: structure of Thermus thermophilus ¹-pyrroline-5-carboxylate dehydrogenase complexed with NADP⁺

E. Inagaki, N. Ohshima, K. Sakamoto, N. D. Babayeva, H. Kato, S. Yokoyama and T. H. Tahirov

Synopsis: A crystal structure of NADP⁺-bound T. thermophilus ¹-pyrroline-5-carboxylate dehydrogenase refined to 1.55 Å resolution is reported. The structure provides structural insights into the mechanism of preference for coenzymes and enzyme activity.

PDB reference: 2ehq

Online 5 May 2007

 

Acta Cryst. (2007). F63, 466-470  [ doi:10.1107/S1744309107024475 ]

Structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a quaternary complex with a magnesium ion, NADPH and the antimalarial drug fosmidomycin

S. Yajima, K. Hara, D. Iino, Y. Sasaki, T. Kuzuyama, K. Ohsawa and H. Seto

Synopsis: The crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) from Escherichia coli complexed with Mg²⁺, NADPH and fosmidomycin was determined at 2.2 Å resolution. The structure showed a well defined loop conformation at the active site of DXR.

PDB reference: 2egh

Online 31 May 2007

 

Acta Cryst. (2007). F63, 471-474  [ doi:10.1107/S174430910702475X ]

Alternative cyanide-binding modes to the haem iron in haem oxygenase

M. Sugishima, K. Oda, T. Ogura, H. Sakamoto, M. Noguchi and K. Fukuyama

Synopsis: Alternative cyanide-binding modes in the haem-haem oxygenase complex are described.

PDB reference: 2e7e

Online 31 May 2007

 

Acta Cryst. (2007). F63, 475-479  [ doi:10.1107/S1744309107017423 ]

Crystallization and crystal properties of squid rhodopsin

M. Murakami, R. Kitahara, T. Gotoh and T. Kouyama

Synopsis: Truncated rhodopsin from the retina of the squid Todarodes pacificus was extracted and crystallized by the sitting-drop vapour-diffusion method. Hexagonal crystals grown in the presence of octylglucoside and ammonium sulfate diffracted to 2.8 Å resolution.

Online 5 May 2007

 

Acta Cryst. (2007). F63, 480-483  [ doi:10.1107/S1744309107020052 ]

Expression, purification, cocrystallization and preliminary crystallographic analysis of sucrose octasulfate/human complement regulator factor H SCRs 6-8

B. E. Prosser, S. Johnson, P. Roversi, S. J. Clark, E. Tarelli, R. B. Sim, A. J. Day and S. M. Lea

Synopsis: The crystallization of human complement regulator FH-678_402H with a glycosaminoglycan analogue is described.

Online 5 May 2007

 

Acta Cryst. (2007). F63, 484-487  [ doi:10.1107/S1744309107020313 ]

Expression, crystallization and preliminary X-ray diffraction studies of recombinant Clostridium perfringens 2-toxin

A. A. Gurjar, N. H. Yennawar, H. P. Yennawar, K. R. Rajashankar, N. V. Hegde and B. M. Jayarao

Synopsis: The cloning, expression, purification and crystallization of recombinant Clostridium perfringens 2-toxin is described. The crystals diffracted to 2.9 Å resolution.

Online 5 May 2007

 

Acta Cryst. (2007). F63, 488-491  [ doi:10.1107/S1744309107020362 ]

Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase

J. Seetharamappa, M. Oke, H. Liu, S. A. McMahon, K. A. Johnson, L. Carter, M. Dorward, M. Zawadzki, I. M. Overton, C. A. J. van Niekirk, S. Graham, C. H. Botting, G. L. Taylor, M. F. White, G. J. Barton, P. J. Coote and J. H. Naismith

Synopsis: Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K.

Online 5 May 2007

 

Acta Cryst. (2007). F63, 492-494  [ doi:10.1107/S1744309107020842 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of branched-chain aminotransferase from Deinococcus radiodurans

C.-D. Chen, T.-F. Huang, C.-H. Lin, H.-H. Guan, Y.-C. Hsieh, Y.-H. Lin, Y.-C. Huang, M.-Y. Liu, W.-C. Chang and C.-J. Chen

Synopsis: The crystallization of branched-chain aminotransferase from D. radiodurans is described.

Online 5 May 2007

 

Acta Cryst. (2007). F63, 495-498  [ doi:10.1107/S1744309107020416 ]

Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase

I. Jabafi, B. Selisko, B. Coutard, A. M. De Palma, J. Neyts, M.-P. Egloff, S. Grisel, K. Dalle, V. Campanacci, S. Spinelli, C. Cambillau, B. Canard and A. Gruez

Synopsis: The first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported.

Online 12 May 2007

 

Acta Cryst. (2007). F63, 499-502  [ doi:10.1107/S174430910702163X ]

Crystallization and preliminary X-ray diffraction studies of the ferredoxin reductase component in the Rieske nonhaem iron oxygenase system carbazole 1,9a-dioxygenase

Y. Ashikawa, H. Uchimura, Z. Fujimoto, K. Inoue, H. Noguchi, H. Yamane and H. Nojiri

Synopsis: The NAD(P)H:ferredoxin oxidoreductase in carbazole 1,9a-dioxygenase from Janthinobacterium sp. J3 was crystallized and diffraction data were collected to 2.60 Å resolution.

Online 12 May 2007

 

Acta Cryst. (2007). F63, 503-506  [ doi:10.1107/S1744309107022750 ]

Crystallization and preliminary X-ray analysis of the complex between a Bacillus subtilis /-type small acid-soluble spore protein and DNA

D. Bumbaca, J. Kosman, P. Setlow, R. K. Henderson and M. J. Jedrzejas

Synopsis: An /-type small, acid-soluble spore protein (SASP) from Bacillus subtilis, a major source of DNA protection against damaging effects in spores, was crystallized in a functionally relevant complex with a double-stranded DNA. This report provides insights into initial characterization of the complex and its structure elucidation.

Online 12 May 2007

 

Acta Cryst. (2007). F63, 507-511  [ doi:10.1107/S1744309107022506 ]

Reductive methylation to improve crystallization of the putative oxidoreductase Rv0765c from Mycobacterium tuberculosis

W. Rauert, A. N. Eddine, S. H. E. Kaufmann, M. S. Weiss and R. Janowski

Synopsis: The M. tuberculosis protein Rv0765c was cloned, expressed, purified and crystallized. In an attempt to improve the quality of the crystals of Rv0765c, the protein was modified by reductive methylation. The methylated protein crystallized in a new crystal form with profoundly improved diffraction properties.

Online 12 May 2007

 

Acta Cryst. (2007). F63, 512-515  [ doi:10.1107/S1744309107022154 ]

Crystallization and preliminary crystallographic analysis of cysteine synthase from Entamoeba histolytica

C. Krishna, R. Jain, T. Kashav, D. Wadhwa, N. Alam and S. Gourinath

Synopsis: A preliminary crystallographic study of cysteine synthase, a major enzyme in the cysteine-biosynthesis pathway, from the amoebic pathogen E. histolytica.

Online 12 May 2007

 

Acta Cryst. (2007). F63, 516-519  [ doi:10.1107/S1744309107022129 ]

Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: purification, crystallization and preliminary X-ray analysis

C. Coelho, P. J. González, J. Trincão, A. L. Carvalho, S. Najmudin, T. Hettman, S. Dieckman, J. J. G. Moura, I. Moura and M. J. Romão

Synopsis: Crystals of the oxidized form of the periplasmic nitrate reductase from Cupriavidus necator were obtained using polyethylene glycol 3350 as precipitant

Online 12 May 2007

 

Acta Cryst. (2007). F63, 520-523  [ doi:10.1107/S1744309107023007 ]

Crystallization and preliminary X-ray analysis of the electron-transfer complex of Rieske-type [2Fe-2S] ferredoxin and NADH-dependent ferredoxin reductase derived from Acidovorax sp. strain KKS102

M. Senda, S. Kishigami, S. Kimura and T. Senda

Synopsis: The electron-transfer complex of BphA3, a Rieske-type [2Fe-2S] ferredoxin, and BphA4, a NADH-dependent ferredoxin reductase, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions.

Online 18 May 2007

 

Acta Cryst. (2007). F63, 524-528  [ doi:10.1107/S1744309107022361 ]

Crystallization and preliminary X-ray diffraction of the Munc18c-syntaxin4_1-29 complex

C. F. Latham, S.-H. Hu, C. L. Gee, C. J. Armishaw, P. F. Alewood, D. E. James and J. L. Martin

Synopsis: Cocrystallization with a peptide, free-interface diffusion crystal chips and crystal dehydration were important in the production of diffraction-quality crystals of the Munc18c protein that helps to regulate membrane fusion.

Online 18 May 2007

 

Acta Cryst. (2007). F63, 529-531  [ doi:10.1107/S1744309107022579 ]

Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain

N. Shibata, Y. Tomimoto, T. Hanamura, R. Yamamoto, M. Ueda, Y. Ueda, N. Mizuno, H. Ogata, H. Komori, Y. Shomura, M. Kataoka, S. Shimizu, J. Kondo, H. Yamamoto, A. Kikuchi and Y. Higuchi

Synopsis: The DIX domain of rat axin has been purified and crystallized. Crystals diffracted to 2.9 Å resolution using synchrotron radiation.

Online 18 May 2007

 

Acta Cryst. (2007). F63, 532-534  [ doi:10.1107/S1744309107024487 ]

Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins

M. Shirokane, Y. Sawano, K. Miyazono, K. Nagata and M. Tanokura

Synopsis: PH1010, a DUF54-family protein from the hyperthermophilic archaeon P. horikoshii OT3, was crystallized and X-ray diffraction data were collected to 1.90 Å resolution.

Online 31 May 2007

 

Acta Cryst. (2007). F63, 535-537  [ doi:10.1107/S1744309107024712 ]

Crystallization and preliminary X-ray analysis of the Ca²⁺-bound C-terminal lobe of troponin C in complex with a troponin I-derived peptide fragment from Akazara scallop

F. Yumoto, K. Nagata, Y. Miyauchi, T. Ojima, H. Tanaka, K. Nishita, I. Ohtsuki and M. Tanokura

Synopsis: Recombinant TnC was expressed in Escherichia coli, purified, complexed with a 24-residue synthetic peptide derived from scallop troponin I (TnI) and crystallized.

Online 31 May 2007

 

Acta Cryst. (2007). F63, 538-541  [ doi:10.1107/S1744309107023391 ]

Crystallization and preliminary X-ray crystallographic studies of the [NiFe] hydrogenase maturation proteins HypC and HypD

S. Watanabe, R. Matsumi, H. Atomi, T. Imanaka and K. Miki

Synopsis: The [NiFe] hydrogenase maturation proteins HypC and HypD were purified and crystallized. Crystals of HypC and HypD suitable for data collection diffracted to 1.80 and 2.07 Å resolution, respectively.

Online 31 May 2007

 

Acta Cryst. (2007). F63, 542-545  [ doi:10.1107/S1744309107022762 ]

Crystallization and preliminary crystallographic characterization of the origin-binding domain of the bacteriophage O replication initiator

E. B. Struble, A. G. Gittis, M. A. Bianchet and R. McMacken

Synopsis: Crystallization and preliminary diffraction data of the N-terminal 19-139 fragment of the origin-binding domain of bacteriophage O replication initiator are reported.

Online 31 May 2007