Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 546-548  [ doi:10.1107/S1744309107028576 ]

Structure of the ThDP-dependent enzyme benzaldehyde lyase refined to 1.65 Å resolution

A. Maraite, T. Schmidt, M. B. Ansörge-Schumacher, A. M. Brzozowski and G. Grogan

Synopsis: The X-ray crystal structure of the ThDP-dependent enzyme benzaldehyde lyase has been refined to 1.65 Å.

PDB reference: 2uz1

Online 15 June 2007

 

Acta Cryst. (2007). F63, 549-552  [ doi:10.1107/S1744309107024049 ]

Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 Å resolution

L.-L. Clancy Kelley, B. D. Dillard, W. Tempel, L. Chen, N. Shaw, D. Lee, M. G. Newton, F. J. Sugar, F. E. Jenney, H. S. Lee, C. Shah, F. L. Poole, M. W. W. Adams, J. S. Richardson, D. C. Richardson, Z.-J. Liu, B.-C. Wang and J. Rose

Synopsis: The crystal structure of the hypothetical protein PF0899 from P. furiosus has been determined to 1.85 Å resolution.

PDB reference: 2pk8

Online 11 June 2007

 

Acta Cryst. (2007). F63, 553-555  [ doi:10.1107/S1744309107023512 ]

Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus solfataricus phosphotriesterase

M. Elias, J. Dupuy, L. Merone, C. Lecomte, M. Rossi, P. Masson, G. Manco and E. Chabriere

Synopsis: A phosphotriesterase (PTE) from the hyperthermophilic archaeon S. solfataricus has been crystallized. Combined with biochemical and bioengineering studies, it is expected that the structure of this protein will provide insight into the natural function of the PTE family and provide important data for achieving an efficient organophosphate biodecontaminant.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 556-559  [ doi:10.1107/S174430910702492X ]

Crystallization and preliminary X-ray analysis of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8

S.-H. Kim, H. Miyatake, T. Hisano, W. Iwasaki, A. Ebihara and K. Miki

Synopsis: Crystals of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase from T. thermophilus HB8 were obtained which diffracted synchrotron X-rays to a resolution of 1.6 Å.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 560-562  [ doi:10.1107/S1744309107024906 ]

Crystallization and preliminary X-ray crystallographic analysis of BxlE, a xylobiose transporter from Streptomyces thermoviolaceus OPC-520

K. Seike, J. Sato, K. Tomoo, T. Ishida, A. Yamano, S. Ikenishi, K. Miyamoto and H. Tsujibo

Synopsis: To clarify the structural basis of sugar binding by BxlE at the atomic level, recombinant BxlE was crystallized using the hanging-drop vapour-diffusion method at 290 K.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 563-567  [ doi:10.1107/S1744309107025195 ]

Overexpression, purification, crystallization and preliminary structural studies of catabolic ornithine transcarbamylase from Lactobacillus hilgardii

B. de las Rivas, H. Rodríguez, I. Angulo, R. Muñoz and J. M. Mancheño

Synopsis: The catabolic ornithine transcarbamylase (cOTC) from L. hilgardii has been overexpressed in E. coli, purified and crystallized under two different experimental conditions. The structure has been solved by the molecular-replacement method using the atomic coordinates of catabolic ornithine transcarbamylase from P. aeruginosa as the search model.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 568-570  [ doi:10.1107/S174430910702533X ]

The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis

M. J. Sánchez-Barrena, S. Moreno-Pérez, I. Angulo, M. Martínez-Ripoll and A. Albert

Synopsis: Recombinant SOS3 and SOS2 regulatory domain from A. thaliana have been coexpressed in E. coli, purified and crystallized by the hanging-drop vapour-diffusion method. An X-ray data set has been collected at 2.0 Å resolution.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 571-575  [ doi:10.1107/S1744309107026103 ]

Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide

J. Wolfová, J. R. Mesters, J. Brynda, R. Grandori, A. Natalello, J. Carey and I. Kutá Smatanová

Synopsis: E. coli WrbA, the founding member of a novel flavoprotein family, was crystallized in complex with its physiological cofactor. Preliminary diffraction analysis is reported.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 576-578  [ doi:10.1107/S1744309107026164 ]

Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata

H. Morita, S. Kondo, R. Kato, K. Wanibuchi, H. Noguchi, S. Sugio, I. Abe and T. Kohno

Synopsis: An acridone-producing novel type III polyketide synthase from H. serrata has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.0 Å.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 579-581  [ doi:10.1107/S1744309107026152 ]

Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase

S. Kim, S. Joo, H. C. Yoon, Y. Ryu, K. K. Kim and T. D. Kim

Synopsis: Est25, a ketoprofen-specific hormone-sensitive lipase from a metagenomic library, was crystallized and diffraction data were collected to 1.49 Å resolution.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 582-585  [ doi:10.1107/S174430910702622X ]

Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli

M. Lapkouski, S. Panjikar, I. Kuta Smatanova and E. Csefalvay

Synopsis: The purification, crystallization and preliminary diffraction analysis of the HsdR subunit of the EcoR124I endonuclease are described.

Online 11 June 2007

 

Acta Cryst. (2007). F63, 586-589  [ doi:10.1107/S1744309107027273 ]

Expression, purification and preliminary X-ray characterization of DL-2-haloacid dehalogenase from Methylobacterium sp. CPA1

R. Omi, K. Jitsumori, T. Yamauchi, S. Ichiyama, T. Kurihara, N. Esaki, N. Kamiya, K. Hirotsu and I. Miyahara

Synopsis: A recombinant form of DL-2-haloacid dehalogenase from Methylobacterium sp. CPA1 has been expressed in E. coli, purified and crystallized. The crystal belongs to space group P6₃. Diffraction data have been collected to 1.75 Å resolution.

Online 15 June 2007

 

Acta Cryst. (2007). F63, 590-592  [ doi:10.1107/S1744309107028102 ]

Crystallization, diffraction data collection and preliminary crystallographic analysis of DING protein from Pseudomonas fluorescens

S. Moniot, M. Elias, D. Kim, K. Scott and E. Chabriere

Synopsis: Crystallization of DING protein from P. fluorescens is reported. A complete data set was collected to 1.43 Å resolution.

Online 15 June 2007

 

Acta Cryst. (2007). F63, 593-595  [ doi:10.1107/S1744309107028308 ]

Crystallization and preliminary X-ray diffraction analysis of P30, the transmembrane domain of pertactin, an autotransporter from Bordetella pertussis

Y. Zhu, I. Black, A. W. Roszak and N. W. Isaacs

Synopsis: P30, the transmembrane C-terminal domain of pertactin from B. pertussis has been crystallized after refolding in vitro. Preliminary X-ray crystallographic data are reported.

Online 15 June 2007

 

Acta Cryst. (2007). F63, 596-598  [ doi:10.1107/S1744309107028722 ]

Crystallization and preliminary X-ray analysis of BigR, a transcription repressor from Xylella fastidiosa involved in biofilm formation

R. L. Barbosa, F. C. Rinaldi, B. G. Guimarães and C. E. Benedetti

Synopsis: In order to gain new insights into the protein structure and its possible interaction with a metal ion or effector ligand, BigR from X. fastidiosa was crystallized in native and selenomethionine (SeMet) labelled forms using the hanging-drop vapour-diffusion method.

Online 15 June 2007

 

Acta Cryst. (2007). F63, 599-601  [ doi:10.1107/S1744309107028655 ]

Design, synthesis and crystallization of a novel glucagon analog as a therapeutic agent

P. Li, T. Rogers, D. Smiley, R. D. DiMarchi and F. Zhang

Synopsis: The synthesis and crystallization of glucagon-Cex are reported.

Online 15 June 2007

 

Acta Cryst. (2007). F63, 602-604  [ doi:10.1107/S1744309107028783 ]

Expression, purification and crystallization of a human tau-tubulin kinase 2 that phosphorylates tau protein

M. Kitano-Takahashi, H. Morita, S. Kondo, K. Tomizawa, R. Kato, M. Tanio, Y. Shirota, H. Takahashi, S. Sugio and T. Kohno

Synopsis: The kinase domain (residues 1-331) of human tau-tubulin kinase 2 was expressed in insect cells, purified and crystallized. Diffraction data have been collected to 2.9 Å resolution.

Online 15 June 2007

 

Acta Cryst. (2007). F63, 605-607  [ doi:10.1107/S1744309107026073 ]

Crystallization and preliminary X-ray diffraction analysis of a novel Arg49 phospholipase A₂ homologue from Zhaoermia mangshanensis venom

M. T. Murakami, U. Kuch, D. Mebs and R. K. Arni

Synopsis: A single crystal of zhaoermiatoxin with maximum dimensions of 0.2 × 0.2 × 0.5 mm was used for X-ray diffraction data collection to a resolution of 2.05 Å using synchrotron radiation and the diffraction pattern was indexed in the hexagonal space group P6₄, with unit-cell parameters a = 72.9, b = 72.9, c = 93.9 Å.

Online 22 June 2007

 

Acta Cryst. (2007). F63, 608-612  [ doi:10.1107/S1744309107026905 ]

Crystallization and preliminary X-ray crystallographic study of a putative aspartyl-tRNA synthetase from the crenarchaeon Sulfolobus tokodaii strain 7

K. Suzuki, Y. Sato, Y. Maeda, S. Shimizu, M. T. Hossain, S. Ubukata, T. Sekiguchi and A. Takénaka

Synopsis: A putative nondiscriminating aspartyl-tRNA synthetase from the crenarchaeon S. tokodaii strain 7 has been recombinantly expressed, purified and crystallized. The crystal structure has been preliminarily solved at 2.3 Å resolution by the molecular-replacement method.

Online 22 June 2007

 

Acta Cryst. (2007). F63, 613-615  [ doi:10.1107/S1744309107028473 ]

Purification, crystallization and preliminary X-ray diffraction of the C-terminal bromodomain from human BRD2

T. Umehara, M. Wakamori, A. Tanaka, B. Padmanabhan and S. Yokoyama

Synopsis: The C-terminal bromodomain of human BRD2 was cloned, expressed, purified and crystallized. A complete diffraction data set has been collected to 1.80 Å resolution.

Online 22 June 2007

 

Acta Cryst. (2007). F63, 616-619  [ doi:10.1107/S1744309107027121 ]

Crystallization and crystallographic analysis of yeast Sec2p, a guanine nucleotide-exchange factor for the yeast Rab GTPase Sec4p

Y. Sato, S. Fukai and O. Nureki

Synopsis: Crystal structure of a triple mutant (M115L, K121M, T142M) of SeMet-labelled Sec2p, a GEF for Rab GTPase, has been solved by SAD. Control of the selenium sites by mutagenesis much improved the resolution of the SeMet-labelled crystals.

Online 22 June 2007

 

Acta Cryst. (2007). F63, 620-623  [ doi:10.1107/S1744309107025286 ]

Crystallization and preliminary X-ray analysis of phage Mu activator protein C in a complex with promoter DNA

K. K. Shanmuganatham, M. Ravichandran, M. M. Howe and H.-W. Park

Synopsis: The isolation and preliminary X-ray analysis of crystals of phage Mu activator protein C bound to promoter DNA are reported.

Online 22 June 2007

 

Acta Cryst. (2007). F63, 624-626  [ doi:10.1107/S1744309107029351 ]

Expression, purification and crystallization of two major envelope proteins from white spot syndrome virus

X. Tang and C. L. Hew

Synopsis: The crystallization of the N-terminal transmembrane region-truncated VP26 and VP28 of white spot syndrome virus is described.

Online 22 June 2007

 

Acta Cryst. (2007). F63, 627-630  [ doi:10.1107/S1744309107030059 ]

Expression, purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of metabotropic glutamate receptor 7

T. Muto, D. Tsuchiya, K. Morikawa and H. Jingami

Synopsis: The ligand-binding domain of metabotropic glutamate receptor 7 has been overexpressed, purified, and crystallized by the hanging-drop vapour-diffusion method. A complete data set has been collected to 3.30 Å.

Online 29 June 2007

 

Acta Cryst. (2007). F63, 631-634  [ doi:10.1107/S1744309107029077 ]

Expression, purification and preliminary X-ray crystallographic analysis of the human major histocompatibility antigen HLA-B*1402 in complex with a viral peptide and with a self-peptide

P. Kumar, A. Vahedi-Faridi, E. Merino, J. A. López de Castro, A. Volz, A. Ziegler, W. Saenger and B. Uchanska-Ziegler

Synopsis: The crystallization of HLA-B*1402 in complex with two peptides is reported.

Online 29 June 2007

 

Acta Cryst. (2007). F63, 635-636  [ doi:10.1107/S1744309107023652 ]

Nanodevices for the Life Sciences

Online 11 June 2007