Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 637-641  [ doi:10.1107/S1744309107033623 ]

The structure of the flexible arm of Thermotoga maritima tRNase Z differs from those of homologous enzymes

R. Ishii, A. Minagawa, H. Takaku, M. Takagi, M. Nashimoto and S. Yokoyama

Synopsis: The crystal structure of T. maritima tRNase Z has been determined at 1.97 Å. The structure shows the flexible arm and the fully metal-binding active site.

PDB reference: 2e7y

Online 21 July 2007

 

Acta Cryst. (2007). F63, 642-647  [ doi:10.1107/S1744309107033726 ]

Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension

W. S. Tan, I. W. McNae, K. L. Ho and M. D. Walkinshaw

Synopsis: Hepatitis B virus capsids have significant potential as carriers for immunogenic peptides. The crystal structure of the T = 4 particle of hepatitis B core protein containing an N-terminal extension reveals that the fusion peptide is exposed on the exterior of the particle.

PDB reference: 2qij

Online 21 July 2007

 

Acta Cryst. (2007). F63, 648-652  [ doi:10.1107/S1744309107033477 ]

High-resolution structures of bacterially expressed soluble human CD59

K. J. Leath, S. Johnson, P. Roversi, T. R. Hughes, R. A. G. Smith, L. Mackenzie, B. P. Morgan and S. M. Lea

Synopsis: The X-ray crystallographic structure of the complement regulator CD59 at 1.15 Å resolution hints at modes of interaction with C8/C9.

PDB references: 2j8b, 2uwr and 2ux2

Online 28 July 2007

 

Acta Cryst. (2007). F63, 653-656  [ doi:10.1107/S1744309107034525 ]

A structural insight into lead neurotoxicity and calmodulin activation by heavy metals

P. Kursula and V. Majava

Synopsis: Crystal structures of human recombinant calmodulin were determined in the presence of lead and barium ions.

PDB references: 2v01 and 2v02

Online 28 July 2007

 

Acta Cryst. (2007). F63, 657-661  [ doi:10.1107/S1744309107031260 ]

Expression, purification, crystallization and preliminary crystallographic analysis of the mouse transcription factor MafB in complex with its DNA-recognition motif Cmare

L. C. Textor, M. Wilmanns and S. J. Holton

Synopsis: The MafB transcription factor (residues 211-305) has been crystallized in complex with the 21 bp Cmare DNA-binding site. Native and mercury-derivatized data collection and preliminary analyses are reported.

Online 7 July 2007

 

Acta Cryst. (2007). F63, 662-664  [ doi:10.1107/S1744309107031363 ]

Purification, crystallization and preliminary crystallographic characterization of the 2,6-sialyltransferase from Photobacterium sp. JT-ISH-224

N. Okino, Y. Kakuta, H. Kajiwara, M. Ichikawa, Y. Takakura, M. Ito and T. Yamamoto

Synopsis: Crystallization of the 2,6-sialyltransferase from Photobacterium.

Online 7 July 2007

 

Acta Cryst. (2007). F63, 665-668  [ doi:10.1107/S1744309107031557 ]

Crystallization and preliminary X-ray analysis of a decameric form of cytosolic thioredoxin peroxidase 1 (Tsa1), C47S mutant, from Saccharomyces cerevisiae

M. A. de Oliveira, V. Genu, K. F. Discola, S. V. Alves, L. E. S. Netto and B. G. Guimarães

Synopsis: A recombinant mutant (C47S) of cytosolic thioredoxin peroxidase 1 from S. cerevisiae was expressed, purified and crystallized by the hanging-drop vapour-diffusion method from protein previously treated with 1,4-dithiothreitol. The crystals belong to the monoclinic space group C2 and diffraction data were collected to 2.8 Å resolution using a synchrotron-radiation source.

Online 7 July 2007

 

Acta Cryst. (2007). F63, 669-672  [ doi:10.1107/S1744309107031764 ]

Crystallization and preliminary crystallographic studies of human kallikrein 7, a serine protease of the multigene kallikrein family

I. S. Fernández, L. Ständker, W.-G. Forssmann, G. Giménez-Gallego and A. Romero

Synopsis: The cloning, expression, purification and crystallization of recombinant human kallikrein 7, directly synthesized in the active form in E. coli, is described. Diffraction data were collected to 2.8 Å resolution from native crystals.

Online 7 July 2007

 

Acta Cryst. (2007). F63, 673-677  [ doi:10.1107/S1744309107032277 ]

Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus

N. Höfer, O. Kolaj, H. Li, V. Cherezov, R. Gillilan, J. G. Wall and M. Caffrey

Synopsis: Diffraction-quality crystals of the apo (1.7 Å) and zinc-bound forms (2.2 Å) of the water-soluble C-terminal domain of the putative zinc transporter CzrB from T. thermophilus have been grown using recombinant production of the protein in E. coli and a combination of vapour-diffusion, batch and seeding crystallogenesis techniques.

Online 21 July 2007

 

Acta Cryst. (2007). F63, 678-681  [ doi:10.1107/S1744309107032344 ]

Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of the Mg²⁺ transporter MgtE

Y. Tanaka, M. Hattori, S. Fukai, R. Ishitania and O. Nureki

Synopsis: Crystals of the cytosolic domain of the Mg²⁺ transporter MgtE from T. thermophilus in the presence and absence of Mg²⁺ diffracted X-rays to 2.3 and 3.5 Å resolution, respectively, and belong to space groups P6₅22 (a = b = 57.7, c = 317.6 Å) and P2₁2₁2₁ (a = 77.0, b = 100.3, c = 100.3 Å), respectively.

Online 21 July 2007

 

Acta Cryst. (2007). F63, 682-684  [ doi:10.1107/S1744309107032332 ]

Crystallization and preliminary X-ray diffraction analysis of the full-length Mg²⁺ transporter MgtE

M. Hattori, Y. Tanaka, S. Fukai, R. Ishitani and O. Nureki

Synopsis: Selenomethionine-substituted crystals of the Mg²⁺ transporter MgtE from T. thermophilus diffracted to 3.5 Å resolution and belonged to space group C222₁, with unit-cell parameters a = 118.3, b = 134.9, c = 366.2 Å.

Online 21 July 2007

 

Acta Cryst. (2007). F63, 685-688  [ doi:10.1107/S1744309107032757 ]

Crystallization and preliminary crystallographic analysis of hygromycin B phosphotransferase from Escherichia coli

D. Iino, Y. Takakura, M. Kuroiwa, R. Kawakami, Y. Sasaki, T. Hoshino, K. Ohsawa, A. Nakamura and S. Yajima

Synopsis: The crystallization and preliminary X-ray studies of the aminoglycoside antibiotic-modifying enzyme hygromycin B phosphotransferase from E. coli are reported.

Online 21 July 2007

 

Acta Cryst. (2007). F63, 689-691  [ doi:10.1107/S1744309107033027 ]

Cloning, expression, crystallization and preliminary X-ray analysis of the first two Ig domains from human Roundabout 1 (Robo1)

C. Morlot, W. Hemrika, R. A. Romijn, P. Gros, S. Cusack and A. A. McCarthy

Synopsis: Robo1 is a large transmembrane receptor expressed on the axon growth cone. Activation of Robo1 by Slit proteins results in axon repulsion from the midline. Here, the purification and crystallization conditions of first two Ig domains of Robo1 are reported.

Online 21 July 2007

 

Acta Cryst. (2007). F63, 692-694  [ doi:10.1107/S1744309107033702 ]

Crystallization and preliminary X-ray analysis of an arabinoxylan arabinofuranohydrolase from Bacillus subtilis

E. Vandermarliere, T. M. Bourgois, S. Van Campenhout, S. V. Strelkov, G. Volckaert, J. A. Delcour, C. M. Courtin and A. Rabijns

Synopsis: The crystallization and preliminary X-ray analysis of the family 43 glycoside hydrolase arabinoxylan arabinofuranohydrolase from B. subtilis soaked with xylotriose is described in order to gain insight in the way the enzyme binds its substrates.

Online 21 July 2007

 

Acta Cryst. (2007). F63, 695-700  [ doi:10.1107/S1744309107033957 ]

Heating of proteins as a means of improving crystallization: a successful case study on a highly amyloidogenic triple mutant of human transthyretin

A. Karlsson and A. E. Sauer-Eriksson

Synopsis: By heating a highly amyloidogenic mutant of the human plasma protein transthyretin at 328 K for 48 h, diffraction-quality crystals could be reproducibly produced. The procedure precipitated 40% of the protein, but rendered what remained in solution more homogenous.

PDB reference: 2qel

Online 21 July 2007

 

Acta Cryst. (2007). F63, 701-703  [ doi:10.1107/S1744309107034239 ]

Crystallization and preliminary X-ray diffraction analysis of hemextin A: a unique anticoagulant protein from Hemachatus haemachatus venom

Y. Banerjee, S. Kumar, C. Jobichen and R. M. Kini

Synopsis: Crystals of hemextin A, a three-finger toxin isolated and purified from African Ringhals cobra (H. haemachatus), are orthorhombic, space group P2₁2₁2₁, with unit-cell parameters a = 49.27, b = 49.51, c = 57.87 Å, and diffract to 1.5 Å resolution.

Online 21 July 2007

 

Acta Cryst. (2007). F63, 704-707  [ doi:10.1107/S1744309107031429 ]

Purification, crystallization and preliminary X-ray diffraction analysis of saxthrombin, a thrombin-like enzyme from Gloydius saxatilis venom

W. Wei, W. Zhao, X. Wang, M. Teng and L. Niu

Synopsis: The thrombin-like enzyme saxthrombin has been purified from G. saxatilis snake venom. Crystallization conditions were found and a data set was obtained to 1.43 Å.

Online 28 July 2007

 

Acta Cryst. (2007). F63, 708-710  [ doi:10.1107/S1744309107034367 ]

Crystallization and preliminary crystallographic studies of LipA, a secretory lipase/esterase from Xanthomonas oryzae pv. oryzae

G. Aparna, A. Chatterjee, G. Jha, R. V. Sonti and R. Sankaranarayanan

Synopsis: The crystallization and preliminary crystallographic studies of LipA, a lipase/esterase secreted by X. oryzae pv. oryzae during its infection of rice plants, are reported.

Online 28 July 2007

 

Acta Cryst. (2007). F63, 711-715  [ doi:10.1107/S174430910703477X ]

Cloning, expression, purification and preliminary crystallographic analysis of the short-chain dehydrogenase enzymes WbmF, WbmG and WbmH from Bordetella bronchiseptica

N. J. Harmer, J. D. King, C. M. Palmer, A. Preston, D. J. Maskell and T. L. Blundell

Synopsis: The expression, purification, and crystallisation of the short-chain dehydrogenases WbmF, WbmG and WbmH from B. bronchiseptica are described. Native diffraction data to 1.5, 2.0, and 2.2 Å were obtained for the three proteins, together with complexes with nucleotides.

Online 28 July 2007

 

Acta Cryst. (2007). F63, 716-719  [ doi:10.1107/S1744309107034483 ]

Binding assay and preliminary X-ray crystallographic analysis of ACTIBIND, a protein with anticarcinogenic and antiangiogenic activities

M. de Leeuw, L. Roiz, P. Smirnoff, B. Schwartz, O. Shoseyov and O. Almog

Synopsis: Native ACTIBIND was successfully crystallized and it was shown that the interaction between ACTIBIND and actin is in a molar ratio of 1:2, with a binding constant of 16.17 × 10⁴ M^-1.

Online 28 July 2007

 

Acta Cryst. (2007). F63, 720-722  [ doi:10.1107/S1744309107033234 ]

Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease

X. Tian, Y. Feng, T. Zhao, H. Peng, J. Yan, J. Qi, F. Jiang, K. Tian and F. Gao

Synopsis: Recombinant PRRSV 3CL protease was crystallized and the crystals diffracted to 2.1 Å resolution.

Online 28 July 2007