Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 723-727  [ doi:10.1107/S1744309107037335 ]

Crystallization and X-ray structure of full-length recombinant human butyrylcholinesterase

M. N. Ngamelue, K. Homma, O. Lockridge and O. A. Asojo

Synopsis: The first crystals and the 2.8 Å X-ray structure of full-length recombinant human butyrylcholinesterase are reported.

PDB reference: 2pm8

Online 10 August 2007

 

Acta Cryst. (2007). F63, 728-733  [ doi:10.1107/S1744309107039565 ]

A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate

S. B. Larson, J. S. Day, R. Cudney and A. McPherson

Synopsis: The structure of bovine pancreatic RNase A has been determined in complex with 2'-deoxyguanosine-5'-monophosphate (dGMP) at 1.33 Å resolution at room temperature in a previously unreported unit cell belonging to space group P3₁.

PDB reference: 2qca

Online 31 August 2007

 

Acta Cryst. (2007). F63, 734-736  [ doi:10.1107/S1744309107033854 ]

Crystallization and preliminary crystallographic studies of the metalloglycoprotein esterase A4 using a baculovirus expression system

T. Hiraki, N. Shibayama, Y.-H. Yoon, K.-M. Yun, T. Hamamoto, J. R. H. Tame and S.-Y. Park

Synopsis: Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation.

Online 10 August 2007

    

Acta Cryst. (2007). F63, 737-739  [ doi:10.1107/S1744309107034793 ]

Crystallization and preliminary X-ray analysis of ginkbilobin-2 from Ginkgo biloba seeds: a novel antifungal protein with homology to the extracellular domain of plant cysteine-rich receptor-like kinases

T. Miyakawa, Y. Sawano, K. Miyazono, K. Hatano and M. Tanokura

Synopsis: Purification and crystallization of ginkbilobin-2 and its selenomethionine derivative allowed the collection of complete data to 2.38 Å resolution and multiwavelength anomalous diffraction data sets, respectively.

Online 10 August 2007

 

Acta Cryst. (2007). F63, 740-741  [ doi:10.1107/S1744309107035658 ]

Purification, crystallization and preliminary X-ray diffraction analysis of water-soluble chlorophyll-binding protein from Chenopodium album

T. Ohtsuki, S. Ohshima and A. Uchida

Synopsis: A water-soluble chlorophyll-binding protein with photoconvertibility from C. album was extracted, purified and crystallized in a darkroom. The crystal diffracted to around 2.0 Å resolution.

Online 10 August 2007

 

Acta Cryst. (2007). F63, 742-745  [ doi:10.1107/S1744309107035865 ]

Crystallization and preliminary X-ray diffraction studies of choline-binding protein F from Streptococcus pneumoniae

R. Molina, A. González, M. Moscoso, P. García, M. Stelter, R. Kahn and J. A. Hermoso

Synopsis: The modular choline-binding protein F (CbpF) from S. pneumoniae has been crystallized by the hanging-drop vapour-diffusion method. A SAD data set from a gadolinium-complex derivative has been collected to 2.1 Å resolution.

Online 10 August 2007

 

Acta Cryst. (2007). F63, 746-750  [ doi:10.1107/S1744309107035816 ]

Crystallization and preliminary X-ray characterization of the Bacillus amyloliquefaciens YwrO enzyme

M. M. AbuKhader, J. Heap, C. I. De Matteis, S. W. Doughty, N. Minton and M. Paoli

Synopsis: The novel bacterial nitroreductase YwrO from B. amyloliquefaciens has been crystallized in two different crystal forms. An initial molecular-replacement solution has been obtained using the mammalian NQO2 structure.

Online 10 August 2007

 

Acta Cryst. (2007). F63, 751-753  [ doi:10.1107/S1744309107036263 ]

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217

J. Wada, R. Suzuki, S. Fushinobu, M. Kitaoka, T. Wakagi, H. Shoun, H. Ashida, H. Kumagai, T. Katayama and K. Yamamoto

Synopsis: Preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from B. longum is described.

Online 10 August 2007

 

Acta Cryst. (2007). F63, 754-757  [ doi:10.1107/S1744309107034458 ]

Preliminary X-ray analysis of cellobiohydrolase Cel7B from Melanocarpus albomyces

T. Parkkinen, A. Koivula, J. Vehmaanperä and J. Rouvinen

Synopsis: The crystallization and preliminary X-ray diffraction analysis of cellobiohydrolase from M. albomyces is reported.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 758-760  [ doi:10.1107/S1744309107037244 ]

Crystallization and preliminary X-ray structural studies of a Melan-A pMHC-TCR complex

F. Yuan, T. Georgiou, T. Hillon, E. Gostick, D. A. Price, A. K. Sewell, R. Moysey, J. Gavarret, A. Vuidepot, M. Sami, J. I. Bell, G. F. Gao, P. J. Rizkallah and B. K. Jakobsen

Synopsis: A preliminary X-ray crystal structural study of a soluble cognate T-cell receptor (TCR) in complex with a pMHC presenting the Melan-A peptide (ELAGIGILTV) is reported. The TCR and pMHC were refolded, purified and mixed together to form complexes, which were crystallized using the sitting-drop vapour-diffusion method. Single TCR-pMHC complex crystals were cryocooled and used for data collection.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 761-764  [ doi:10.1107/S1744309107038651 ]

Purification, crystallization and preliminary X-ray characterization of a human mitochondrial phenylalanyl-tRNA synthetase

I. Levin, N. Kessler, N. Moor, L. Klipcan, E. Koc, P. Templeton, L. Spremulli and M. Safro

Synopsis: The expression, purification and crystallization of recombinant human mitochondrial phenylalanyl-tRNA synthetase (mitPheRS) are reported. Diffraction data were collected to 2.2 Å resolution and the mitPheRS structure was solved using the molecular-replacement method.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 765-767  [ doi:10.1107/S1744309107038833 ]

Crystallization and preliminary X-ray crystallographic study of [NiFe]-hydrogenase maturation factor HypE from Thermococcus kodakaraensis KOD1

T. Arai, S. Watanabe, R. Matsumi, H. Atomi, T. Imanaka and K. Miki

Synopsis: The [NiFe]-hydrogenase maturation protein HypE was purified and crystallized. Crystals of HypE suitable for data collection diffracted to 1.55 Å resolution.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 768-770  [ doi:10.1107/S1744309107038985 ]

Expression, purification, crystallization and preliminary crystallographic analysis of the proliferation-associated protein Ebp1

E. Kowalinski, G. Bange, K. Wild and I. Sinning

Synopsis: Preliminary X-ray analysis of the proliferation-associated protein Ebp1 from Homo sapiens is provided.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 771-773  [ doi:10.1107/S1744309107038948 ]

Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of a cation diffusion facilitator family protein

M. Hattori, Y. Tanaka, R. Ishitani and O. Nureki

Synopsis: Selenomethionine-substituted crystals of the cytosolic domain of the cation diffusion facilitator family protein from T. maritima diffracted X-rays to 2.5 Å and belonged to space group R32, with unit-cell parameters a = b = 97.7, c = 83.4 Å.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 774-776  [ doi:10.1107/S174430910703936X ]

Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase

W. Saburi, H. Hondoh, H. Unno, M. Okuyama, H. Mori, T. Nakada, Y. Matsuura and A. Kimura

Synopsis: Dextran glucosidase from S. mutans was crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.2 Å resolution.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 777-779  [ doi:10.1107/S174430910703953X ]

Crystallization and preliminary crystallographic analysis of an esterase with a novel domain from the hyperthermophile Thermotoga maritima

L. Sun, M. Levisson, S. Hendriks, T. Akveld, S. W. M. Kengen, B. W. Dijkstra and J. van der Oost

Synopsis: A thermostable esterase (EstA) from Thermotoga maritima was cloned and purified. Crystals of EstA and its selenomethionine derivative were grown and diffract to beyond 2.6 Å resolution at 100 K using synchrotron radiation.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 780-783  [ doi:10.1107/S174430910703984X ]

Purification, crystallization and preliminary X-ray diffraction analysis of royal palm tree (Roystonea regia) peroxidase

L. Watanabe, A. S. Nascimento, L. S. Zamorano, V. L. Shnyrov and I. Polikarpov

Synopsis: The purification, crystallization, X-ray diffraction data acquisition and molecular-replacement results of royal palm tree (R. regia) peroxidase are described.

Online 25 August 2007

 

Acta Cryst. (2007). F63, 784-786  [ doi:10.1107/S1744309107038328 ]

Crystallization and preliminary X-ray crystallographic analysis of a 40 kDa N-terminal fragment of the yeast prion-remodeling factor Hsp104

S. Lee and F. T. F. Tsai

Synopsis: An N-terminal fragment of S. cerevisiae Hsp104 has been crystallized. This is the first report of the crystallization of a eukaryotic member of the Hsp100 family of molecular chaperones.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 787-791  [ doi:10.1107/S1744309107039838 ]

Crystallization and identification of the glycosylated moieties of two isoforms of the main allergen Hev b 2 and preliminary X-ray analysis of two polymorphs of isoform II

D. Fuentes-Silva, G. Mendoza-Hernández, V. Stojanoff, L. A. Palomares, E. Zenteno, A. Torres-Larios and A. Rodríguez-Romero

Synopsis: Crystallization of important glycoenzymes involved in IgE-mediated latex allergy.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 792-794  [ doi:10.1107/S1744309107039590 ]

Purification, crystallization and preliminary X-ray analysis of the fumarylacetoacetase family member TTHA0809 from Thermus thermophilus HB8

H. Mizutani and N. Kunishima

Synopsis: The putative fumarylacetoacetase TTHA0809 from T. thermophilus HB8 has been overexpressed, purified and crystallized. The crystals diffracted X-rays to 2.2 Å resolution using synchrotron radiation.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 795-797  [ doi:10.1107/S174430910704016X ]

Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I

G. S. Kachalova, A. K. Yunusova, R. I. Artyukh, E. A. Rogulin, T. A. Perevyazova, L. A. Zheleznaya, N. I. Matvienko and H. D. Bartunik

Synopsis: The crystallization of the small subunit of the heterodimeric restriction endonuclease R.BspD6I and diffraction data collection to 1.5 Å resolution are reported.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 798-801  [ doi:10.1107/S1744309107040134 ]

Purification, crystallization and preliminary X-ray diffraction studies of the soluble domain of the oligosaccharyltransferase STT3 subunit from the thermophilic archaeon Pyrococcus furiosus

M. Igura, N. Maita, T. Obita, J. Kamishikiryo, K. Maenaka and D. Kohda

Synopsis: The C-terminal soluble domain of the catalytic subunit (STT3) of the oligosaccharyltransferase from P. furiosus was purified and crystallized. A native crystal and a SeMet derivative have been analyzed using X-ray diffraction.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 802-806  [ doi:10.1107/S1744309107040341 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of Thermotoga neapolitana -glucosidase B

P. Turner, A. Pramhed, E. Kanders, M. Hedström, E. N. Karlsson and D. T. Logan

Synopsis: Here, the expression, purification, crystallization and X-ray diffraction data of a family 3 -glucosidase from the hyperthermophilic bacterium Thermotoga neapolitana are reported.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 807-808  [ doi:10.1107/S1744309107040298 ]

Crystallization and preliminary crystallographic analysis of D-alanine-D-alanine ligase from Streptococcus mutans

Y.-Z. Lu, Y. Sheng, L.-F. Li, D.-W. Tang, X.-Y. Liu, X. Zhao, Y.-H. Liang and X.-D. Su

Synopsis: A potential target for antibiotic drug design, D-alanine-D-alanine ligase from S. mutans, was expressed in E. coli, purified and crystallized. Diffraction data were collected to 2.4 Å resolution.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 809-811  [ doi:10.1107/S1744309107040304 ]

Protein preparation and preliminary X-ray crystallographic analysis of a putative glucosamine 6-phosphate deaminase from Streptococcus mutants

G.-J. Hu, L.-F. Li, D. Li, C. Liu, S.-C. Wei, Y.-H. Liang and X.-D. Su

Synopsis: A glucosamine 6-phosphate deaminase homologue from S. mutans was expressed, purified and crystallized. Diffraction data have been collected to 2.4 Å resolution.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 812-815  [ doi:10.1107/S1744309107040316 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of Nudix hydrolase Orf141 from Escherichia coli K-1

J. Jung, Y.-J. Ahn and L.-W. Kang

Synopsis: A novel Nudix hydrolase Orf141 from E. coli, which cleaves pyrimidine deoxynucleoside triphosphates, was recently cloned. In order to illuminate the structural and functional features of the novel Nudix hydrolase Orf141, it was expressed, purified and crystallized and a preliminary X-ray crystallographic analysis was conducted.

Online 31 August 2007

 

Acta Cryst. (2007). F63, 816-818  [ doi:10.1107/S1744309107040523 ]

Purification, crystallization and preliminary X-ray analysis of a Nup107-Nup133 heterodimeric nucleoporin complex

T. Boehmer and T. U. Schwartz

Synopsis: A heterodimeric complex consisting of the C-terminal domains of human nucleoporins Nup107 and Nup133 was purified and crystallized and complete diffraction data sets were collected.

Online 31 August 2007