Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 819-824  [ doi:10.1107/S1744309107041139 ]

Structure of Alzheimer's disease amyloid precursor protein copper-binding domain at atomic resolution

G.K.-W. Kong, J. J. Adams, R. Cappai and M. W. Parker

Synopsis: An atomic resolution structure of the copper-binding domain of the Alzheimer's disease amyloid precursor protein is presented.

PDB reference: 2fma

Online 19 September 2007

 

Acta Cryst. (2007). F63, 825-830  [ doi:10.1107/S1744309107040985 ]

Structure of 3(17)-hydroxysteroid dehydrogenase (AKR1C21) holoenzyme from an orthorhombic crystal form: an insight into the bifunctionality of the enzyme

U. Dhagat, V. Carbone, R.P.-T. Chung, C. Schulze-Briese, S. Endo, A. Hara and O. El-Kabbani

Synopsis: The structure of AKR1C21 holoenzyme was determined at 1.8 Å resolution. A model describing the interaction between AKR1C21 and steroid substrates is proposed that explains the bifunctionality of the enzyme.

PDB reference: 2p5n

Online 19 September 2007

 

Acta Cryst. (2007). F63, 831-835  [ doi:10.1107/S1744309107042455 ]

Structure of Mycobacterium tuberculosis mtFabD, a malonyl-CoA:acyl carrier protein transacylase (MCAT)

H. Ghadbane, A. K. Brown, L. Kremer, G. S. Besra and K. Fütterer

Synopsis: Binding of Ni²⁺ ions to the uncleaved affinity tag facilitated de novo phasing of the crystal structure of M. tuberculosis mtFabD to 3.0 Å resolution.

PDB reference: 2qj3

Online 19 September 2007

 

Acta Cryst. (2007). F63, 836-838  [ doi:10.1107/S1744309107040377 ]

Purification, crystallization, X-ray diffraction analysis and phasing of an engineered single-chain PvuII restriction endonuclease

C. Meramveliotaki, D. Kotsifaki, M. Androulaki, A. Hountas, E. Eliopoulos and M. Kokkinidis

Synopsis: PvuII is the first type II restriction endonuclease to be converted from its wild-type homodimeric form into an enzymatically active single-chain variant. The enzyme was crystallized and phasing was successfully performed by molecular replacement.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 839-843  [ doi:10.1107/S1744309107040857 ]

Cocrystallizing natural RNA with its unnatural mirror image: biochemical and preliminary X-ray diffraction analysis of a 5S rRNA A-helix racemate

C. Förster, A. B. E. Brauer, D. Lehmann, T. Borowski, S. Brode, J. P. Fürste, M. Perbandt, C. Betzel and V. A. Erdmann

Synopsis: A 5S rRNA A-helix 7-mer oligonucleotide was chemically synthesized both as D-RNA and as L-RNA, biochemically investigated, crystallized as a stochiometric racemate and examined by X-ray diffraction.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 844-847  [ doi:10.1107/S1744309107041152 ]

Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of adhesion molecule CD44

T. Mori, K. Kitano, S. Terawaki, R. Maesaki and T. Hakoshima

Synopsis: The radixin FERM domain complexed with the CD44 cytoplasmic tail peptide has been crystallized. A diffraction data set from the complex was collected to 2.1 Å.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 848-851  [ doi:10.1107/S1744309107041176 ]

Purification, crystallization and initial crystallographic characterization of peanut major allergen Ara h 3

T. Jin, A. Howard and Y.-Z. Zhang

Synopsis: The crystallization of peanut allergen Ara h 3 is reported.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 852-854  [ doi:10.1107/S1744309107040560 ]

Isolation, crystallization and preliminary crystallographic analysis of Salmonella typhimurium uridine phosphorylase crystallized with 2,2'-anhydrouridine

V. I. Timofeev, A. A. Lashkov, A. G. Gabdoulkhakov, B. P. Pavlyuk, G. S. Kachalova, C. Betzel, E. Y. Morgunova, N. E. Zhukhlistova and A. M. Mikhailov

Synopsis: S. typhimurium uridine phosphorylase has been isolated and crystallized in the presence of ligand.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 855-857  [ doi:10.1107/S1744309107041437 ]

Crystallization and preliminary crystallographic analysis of the ferredoxin component of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177

K. Inoue, Y. Ashikawa, Y. Usami, H. Noguchi, Z. Fujimoto, H. Yamane and H. Nojiri

Synopsis: The ferredoxin component of carbazole 1,9a-dioxygenase from N. aromaticivorans IC177 was crystallized and diffraction data were collected to 2.0 Å resolution.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 858-861  [ doi:10.1107/S1744309107041528 ]

Human tRNA^Gly acceptor-stem microhelix: crystallization and preliminary X-ray diffraction analysis at 1.2 Å resolution

C. Förster, K. Szkaradkiewicz, M. Perbandt, A. B. E. Brauer, T. Borowski, J. P. Fürste, C. Betzel and V. A. Erdmann

Synopsis: The human tRNA^Gly acceptor-stem microhelix was crystallized and preliminary X-ray diffraction analysis revealed diffraction to a resolution of up to 1.2 Å.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 862-864  [ doi:10.1107/S1744309107041814 ]

Crystallization and preliminary X-ray characterization of arylamine N-acetyltransferase C (BanatC) from Bacillus anthracis

B. Pluvinage, I. Li de la Sierra-Gallay, M. Martins, N. Ragunathan, J.-M. Dupret and F. Rodrigues-Lima

Synopsis: Bacillus anthracis arylamine N-acetyltransferase C (BanatC) is an enzyme that metabolizes the drug sulfamethoxazole. Crystals of the purified enzyme that diffract at 1.95 Å are reported.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 865-869  [ doi:10.1107/S1744309107042807 ]

Crystallization and preliminary X-ray diffraction of human interleukin-7 bound to unglycosylated and glycosylated forms of its -receptor

J. Wickham and S. T. R. Walsh

Synopsis: Bacterial and insect cell expression systems have been developed to produce unglycosylated and glycosylated forms of human interleukin-7 (IL-7) and the extracellular domain of its receptor, IL-7R. We report the crystallization and X-ray diffraction of IL-7 complexes to both unglycosylated and glycosylated forms of the IL-7R to 2.7 and 3.0 Å, respectively.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 870-873  [ doi:10.1107/S1744309107043217 ]

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a resuscitation-promoting factor from Mycobacterium tuberculosis

A. Ruggiero, B. Tizzano, A. Geerlof, E. Pedone, C. Pedone, M. Wilmanns and R. Berisio

Synopsis: The first crystallization of a resuscitation-promoting factor has been performed. Multiwavelength anomalous dispersion experiments have been carried out to obtain experimental phases using data at 2.9 Å resolution from a selenomethionine derivative.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 874-877  [ doi:10.1107/S1744309107042984 ]

Crystallization and preliminary X-ray data analysis of -alanine synthase from Drosophila melanogaster

S. Lundgren, B. Andersen, J. Piskur and D. Dobritzsch

Synopsis: -Alanine synthase catalyzes the last step in the reductive degradation pathway for uracil and thymine. Crystals of the recombinant enzyme from D. melanogaster belong to space group C2. Diffraction data to 3.3 Å resolution were collected and analyzed.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 878-881  [ doi:10.1107/S1744309107043400 ]

Purification, crystallization and preliminary structural characterization of the N-terminal region of the human formin-homology protein FHOD1

A. Schulte, A. Rak, O. Pylypenko, D. Ludwig and M. Geyer

Synopsis: The N-terminal region (1-339) of the human FHOD1 protein has been crystallized in two different crystal forms. A crystal of the (C31S,C71S) mutant diffracted to around 2.3 Å resolution.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 882-883  [ doi:10.1107/S1744309107043242 ]

Protein preparation, crystallization and preliminary X-ray crystallographic analysis of SMU.961 protein from the caries pathogen Streptococcus mutans

X.-Z. Gao, L.-F. Li, X.-D. Su, X. Zhao and Y.-H. Liang

Synopsis: The SMU.961 protein from S. mutans was crystallized and preliminary characterization of the crystals, which diffracted to 2.9 Å resolution, shows them to belong to space group C2.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 884-886  [ doi:10.1107/S1744309107043709 ]

Overproduction, purification and preliminary X-ray diffraction analysis of CzcE from Cupriavidus metallidurans CH34

G. Pompidor, A. Zoropogui, R. Kahn and J. Covès

Synopsis: Crystals of the mature form of CzcE from C. metallidurans CH34 were obtained which diffracted synchrotron radiation to 1.96 Å.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 887-889  [ doi:10.1107/S1744309107044296 ]

Crystallization of sheep (Ovis aries) and goat (Capra hircus) haemoglobins under unbuffered low-salt conditions

K. Neelagandan, P. S. Moorthy, M. Balasubramanian and M. N. Ponnuswamy

Synopsis: The haemoglobins from low oxygen affinity species, sheep and goat are crystallized under unbuffered low-salt conditions to explore the possibility of obtaining new crystal forms.

Online 19 September 2007

 

Acta Cryst. (2007). F63, 890-892  [ doi:10.1107/S1744309107041188 ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DsrEFH from Allochromatium vinosum

C. Dahl, A. Schulte and D. H. Shin

Synopsis: DsrEFH from Allochromatium vinosum has been cloned, expressed, purified, and crystallized. A preliminary X-ray study of DsrEFH has been performed with a good quality crystal.

Online 29 September 2007

 

Acta Cryst. (2007). F63, 893-895  [ doi:10.1107/S1744309107039358 ]

Crystallization and preliminary crystallographic analysis of the fourth FAS1 domain of human BigH3

J.-H. Yoo, E. Kim, J. Kim and H.-S. Cho

Synopsis: The crystallization and X-ray diffraction analysis of the fourth FAS1 domain of human BigH3 are reported.

Online 29 September 2007

 

Acta Cryst. (2007). F63, 896-898  [ doi:10.1107/S1744309107044879 ]

Preparation, crystallization and preliminary crystallographic analysis of old yellow enzyme from Trypanosoma cruzi

S. Sugiyama, K. Tokuoka, N. Uchiyama, N. Okamoto, Y. Okano, H. Matsumura, K. Inaka, Y. Urade and T. Inoue

Synopsis: Old yellow enzyme from Trypanosoma cruzi, has been crystallized using the hanging-drop vapour-diffusion method.

Online 29 September 2007

 

Acta Cryst. (2007). F63, 899  [ doi:10.1107/S1744309107044119 ]

Crystallization and preliminary X-ray analysis of ginkbilobin-2 from Ginkgo biloba seeds: a novel antifungal protein with homology to the extracellular domain of plant cysteine-rich receptor-like kinases. Erratum

T. Miyakawa, Y. Sawano, K. Miyazono, K. Hatano and M. Tanokura

Synopsis: An erratum to the paper by Miyakawa et al. [(2007), Acta Cryst. F63, 737-739].

Online 29 September 2007