Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 900-907  [ doi:10.1107/S1744309107041012 ]

The structures of pyruvate oxidase from Aerococcus viridans with cofactors and with a reaction intermediate reveal the flexibility of the active-site tunnel for catalysis

E. C. M. Juan, M. M. Hoque, M. T. Hossain, T. Yamamoto, S. Imamura, K. Suzuki, T. Sekiguchi and A. Takénaka

Synopsis: The crystal structures of pyruvate oxidase from A. viridans in complex with flavin adenine dinucleotide, thiamine diphosphate and the reaction intermediate 2-acetyl-thiamine diphosphate reveal details of substrate recognition and catalysis.

PDB references: 2dji, 1v5f and 1v5g

Online 20 October 2007

 

Acta Cryst. (2007). F63, 908-913  [ doi:10.1107/S1744309107047720 ]

Structure of Staphylococcus aureus 1,4-dihydroxy-2-naphthoyl-CoA synthase (MenB) in complex with acetoacetyl-CoA

V. Ulaganathan, M. F. Agacan, L. Buetow, L. B. Tulloch and W. N. Hunter

Synopsis: The structure of S. aureus MenB, an enzyme in the biosynthetic pathway to vitamin K₂, has been determined and compared with the enzyme derived from another important pathogen, M. tuberculosis.

PDB reference: 2uzf

Online 20 October 2007

 

Acta Cryst. (2007). F63, 914-917  [ doi:10.1107/S1744309107048166 ]

A high-resolution structure of the DNA-binding domain of AhrC, the arginine repressor/activator protein from Bacillus subtilis

J. A. Garnett, S. Baumberg, P. G. Stockley and S. E. V. Phillips

Synopsis: The structure of the winged helix-turn-helix DNA-binding domain of AhrC has been determined at 1.0 Å resolution. The largely hydrophobic -wing shows high B factors and may mediate the dimer interface in operator complexes.

PDB reference: 2p5k

Online 20 October 2007

 

Acta Cryst. (2007). F63, 918-921  [ doi:10.1107/S1744309107049391 ]

Structure of the C-terminal effector-binding domain of AhrC bound to its corepressor L-arginine

J. A. Garnett, S. Baumberg, P. G. Stockley and S. E. V. Phillips

Synopsis: The crystal structure of the C-terminal domain hexameric core of AhrC, with bound corepressor (L-arginine), has been solved at 1.95 Å resolution. Binding of L-arginine results in a rotation between the two trimers of the hexamer, leading to the activation of the DNA-binding state.

PDB reference: 2p5m

Online 20 October 2007

 

Acta Cryst. (2007). F63, 922-928  [ doi:10.1107/S1744309107048609 ]

Structure of the ribosomal interacting GTPase YjeQ from the enterobacterial species Salmonella typhimurium

C. E. Nichols, C. Johnson, H. K. Lamb, M. Lockyer, I. G. Charles, A. R. Hawkins and D. K. Stammers

Synopsis: The X-ray crystal structure of the GTPase YjeQ from S. typhimurium is presented and compared with those of orthologues from T. maritima and B. subtilis.

PDB reference: 2rcn

Online 24 October 2007

 

Acta Cryst. (2007). F63, 929-931  [ doi:10.1107/S1744309107045393 ]

Crystallization and preliminary crystallographic studies of Schizolobium parahyba chymotrypsin inhibitor (SPCI) at 1.8 Å resolution

R. C. L. Teles, G. F. Esteves, M. A. M. Araújo, C. Bloch, J. A. R. G. Barbosa and S. M. de Freitas

Synopsis: Crystallization and preliminary crystallographic studies of Schizolobium parahyba chymotrypsin inhibitor (SPCI) at 1.8 Å resolution.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 932-935  [ doi:10.1107/S1744309107046015 ]

Purification, crystallization and preliminary X-ray analysis of a thermostable glycoside hydrolase family 43 -xylosidase from Geobacillus thermoleovorans IT-08

A. Rohman, N. van Oosterwijk, S. Kralj, L. Dijkhuizen, B. W. Dijkstra and N. N. T. Puspaningsih

Synopsis: The -xylosidase was crystallized using PEG 6000 as precipitant. 5% PEG 6000 yielded bipyramid-shaped tetragonal crystals diffracting to 1.55 Å resolution, and 13% PEG 6000 gave rectangular monoclinic crystals diffracting to 1.80 Å resolution.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 936-939  [ doi:10.1107/S1744309107046374 ]

Expression, purification and preliminary X-ray analysis of the C-terminal domain of an arginine repressor protein from Mycobacterium tuberculosis

G. J. Lu, C. R. Garen, M. M. Cherney, L. T. Cherney, C. Lee and M. N. G. James

Synopsis: The C-terminal portion of the arginine repressor protein from M. tuberculosis H37Rv has been crystallized. The complete transcriptional factor regulates arginine biosynthesis by binding operator DNA when arginine is bound at the C-terminal domain.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 940-943  [ doi:10.1107/S1744309107046489 ]

Expression, purification, crystallization and preliminary X-ray analysis of the Met244Ala variant of catalase-peroxidase (KatG) from the haloarchaeon Haloarcula marismortui

T. Ten-i, T. Kumasaka, W. Higuchi, S. Tanaka, K. Yoshimatsu, T. Fujiwara and T. Sato

Synopsis: The Met244Ala variant of the H. marismortui KatG enzyme was expressed in haloarchaeal host cells and purified to homogeneity. The variant was crystallized using the hanging-drop vapour-diffusion method with ammonium sulfate and NaCl as precipitants. The reddish-brown rod-shaped crystals obtained belong to the monoclinic space group C2, with unit-cell parameters a = 315.24, b = 81.04, c = 74.77 Å, = 99.81°.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 944-946  [ doi:10.1107/S1744309107046386 ]

Expression, purification, crystallization and initial crystallographic characterization of the p-hydroxybenzoate hydroxylase from Corynebacterium glutamicum

S.-Y. Kwon, B. S. Kang, G.-H. Kim and K.-J. Kim

Synopsis: PHBH from Corynebacterium glutamicum was crystallized using the hanging-drop vapour-diffusion method in the presence of NaH₂PO₄ and K₂HPO₄ as precipitants. X-ray diffraction data were collected to a maximum resolution of 2.5 Å on a synchrotron beamline.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 947-949  [ doi:10.1107/S1744309107047069 ]

Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase

H. Morita, S. Kondo, R. Kato, K. Wanibuchi, H. Noguchi, S. Sugio, I. Abe and T. Kohno

Synopsis: Octaketide synthase from A. arborescens has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.6 Å.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 950-952  [ doi:10.1107/S1744309107048142 ]

Purification, crystallization and preliminary X-ray diffraction study of human ribosomal protein L10 core domain

M. Nishimura, T. Kaminishi, M. Kawazoe, M. Shirouzu, C. Takemoto, S. Yokoyama, A. Tanaka, S. Sugano, T. Yoshida, T. Ohkubo and Y. Kobayashi

Synopsis: A truncated variant of human ribosomal protien L10 was prepared and crystallized. Diffraction data were collected to 2.5 Å resolution.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 953-956  [ doi:10.1107/S174430910704821X ]

Expression and crystallization of DsbA from Staphylococcus aureus

B. Heras, M. Kurz, R. Jarrott, K. A. Byriel, A. Jones, L. Thöny-Meyer and J. L. Martin

Synopsis: Free-interface diffusion crystallization chips were used to identify crystallization conditions for S. aureus DsbA, representing the first Gram-positive DsbA to be crystallized. Native and selenomethionine-derivative crystals diffracted to 2.1 and 2.4 Å resolution, respectively.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 957-960  [ doi:10.1107/S1744309107048610 ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of universal stress protein F (YnaF) from Salmonella typhimurium

S. R. Sagurthi, R. R. Panigrahi, G. Gowda, H. S. Savithri and M. R. N. Murthy

Synopsis: The cloning, purification and crystallization of YnaF from S. typhimurium are reported along with preliminary X-ray crystallographic studies.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 961-963  [ doi:10.1107/S1744309107049159 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)

B. A. Cromer, M. A. Gorman, G. Hansen, J. J. Adams, M. Coggan, P. G. Board and M. W. Parker

Synopsis: Chloride intracellular channel 2 (CLIC2) belongs to a family of intracellular chloride-channel proteins that can exist in a soluble form. The expression, purification and crystallization in two different crystal forms of human CLIC2 is reported.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 964-966  [ doi:10.1107/S1744309107049962 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of ST1022, a putative member of the Lrp/AsnC family of transcriptional regulators isolated from Sulfolobus tokodaii strain 7

N. Nakano, T. Kumarevel, E. Matsunaga, A. Shinkai, S. Kuramitsu and S. Yokoyama

Synopsis: A putative member of the Lrp/AsnC family of transcriptional regulators, ST1022 from S. tokodaii strain 7, has been purified and crystallized in the absence and presence of the effector L-glutamine. A molecular-replacement solution was found using the FL11 transcriptional regulator from Pyrococcus sp. OT3 as a model and structural refinement is under way.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 967-971  [ doi:10.1107/S1744309107050142 ]

Crystallization and preliminary crystallographic analysis of recombinant human galectin-1

S. A. Scott, K. Scott and H. Blanchard

Synopsis: Human galectin-1 has been cloned, expressed in E. coli, purified and crystallized in the presence of both lactose (ligand) and -mercaptoethanol under six different conditions. The X-ray diffraction data obtained have enabled the assignment of unit-cell parameters for two novel crystal forms of human galectin-1.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 972-975  [ doi:10.1107/S1744309107050993 ]

Improving protein crystal quality by selective removal of a Ca²⁺-dependent membrane-insertion loop

D. B. Neau, N. C. Gilbert, S. G. Bartlett, A. Dassey and M. E. Newcomer

Synopsis: Protein engineering dramatically enhances the quality of crystals of a Ca²⁺-dependent membrane-binding protein.

Online 24 October 2007

 

Acta Cryst. (2007). F63, 976-979  [ doi:10.1107/S1744309107051445 ]

Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2

F. Guo, T. Jin, A. Howard and Y.-Z. Zhang

Synopsis: The crystallization of the brazil nut allergen Ber e 2 is reported.

Online 26 October 2007

 

Acta Cryst. (2007). F63, 980-982  [ doi:10.1107/S1744309107051640 ]

Expression, purification and preliminary X-ray diffraction studies of RebC

L. M. van Staalduinen, A. Bhattacharya, K. Groom, D. L. Zechel and Z. Jia

Synopsis: The flavin-dependent monooxygenase RebC has been crystallized by the hanging-drop vapour-diffusion method and initial X-ray diffraction analysis has been completed.

Online 26 October 2007

 

Acta Cryst. (2007). F63, 983-986  [ doi:10.1107/S1744309107051524 ]

Crystallization and preliminary X-ray diffraction studies of tetrameric malate dehydrogenase from the novel Antarctic psychrophile Flavobacterium frigidimaris KUC-1

T. Fujii, T. Oikawa, I. Muraoka, K. Soda and Y. Hata

Synopsis: A psychrophilic malate dehydrogenase from the novel Antarctic bacterium F. frigidimaris KUC-1 was crystallized using the hanging-drop vapour-diffusion method. The crystals contained one tetrameric molecule per asymmetric unit. The best crystal diffracted to 1.8 Å resolution.

Online 26 October 2007

 

Acta Cryst. (2007). F63, 987-989  [ doi:10.1107/S1744309107052256 ]

Purification, crystallization and preliminary X-ray analysis of struthiocalcin 1 from ostrich (Struthio camelus) eggshell

J. P. Reyes-Grajeda, L. Marín-García, V. Stojanoff and A. Moreno

Synopsis: The purification, crystallization and preliminary X-ray diffraction data of the protein struthiocalcin 1 isolated from ostrich eggshell are reported.

Online 26 October 2007

 

Acta Cryst. (2007). F63, 990-992  [ doi:10.1107/S1744309107052670 ]

Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase

E. Kiyota, S. M. de Sousa, M. L. dos Santos, A. da Costa Lima, M. Menossi, J. A. Yunes and R. Aparicio

Synopsis: Preliminary X-ray diffraction studies of apo maize aldose reductase at 2.0 Å resolution are reported.

Online 26 October 2007