Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 63, Part 12 (December 2007)

crystallization communications

Acta Cryst. (2007). F63, 1021-1025 [ doi:10.1107/S1744309107051408 ]

The production and crystallization of the human leukocyte antigen class II molecules HLA-DQ2 and HLA-DQ8 complexed with deamidated gliadin peptides implicated in coeliac disease

K. N. Henderson, H. H. Reid, N. A. Borg, S. E. Broughton, T. Huyton, R. P. Anderson, J. McCluskey and J. Rossjohn

Abstract: The major histocompatibility complex (MHC) class II molecules HLA-DQ2 and HLA-DQ8 are key risk factors in coeliac disease, as they bind deamidated gluten peptides that are subsequently recognized by CD4⁺ T cells. Here, the production and crystallization of both HLA-DQ2 and HLA-DQ8 in complex with the deamidated gliadin peptides DQ2 [alpha] -I (PQPELPYPQ) and DQ8 [alpha] -I (EGSFQPSQE), respectively, are reported.

Keywords: human leukocyte antigen class II molecules; HLA-DQ2; HLA-DQ8.


	PDF file (144.7 kbytes) [ doi:10.1107/S1744309107051408/en5259sup1.pdf ] Sequence details.

	PDF file (40.4 kbytes) [ doi:10.1107/S1744309107051408/en5259sup2.pdf ] Cloning procedure.

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