Structural Biology and Crystallization Communications

 

Acta Cryst. (2007). F63, 993-997  [ doi:10.1107/S1744309107061106 ]

Structure of the minimized /-hydrolase fold protein from Thermus thermophilus HB8

Y. Xie, C. Takemoto, S. Kishishita, T. Uchikubo-Kamo, K. Murayama, L. Chen, Z.-J. Liu, B.-C. Wang, M. Manzoku, A. Ebihara, S. Kuramitsu, M. Shirouzu and S. Yokoyama

Synopsis: The crystal structure of the minimized /-hydrolase fold protein encoded by the gene TTHA1544 from T. thermophilus HB8 has been determined at 2.0 Å resolution.

PDB reference: 2dst

Online 30 November 2007

 

Acta Cryst. (2007). F63, 998-1002  [ doi:10.1107/S1744309107050920 ]

The structure of allophycocyanin from Thermosynechococcus elongatus at 3.5 Å resolution

J. W. Murray, K. Maghlaoui and J. Barber

Synopsis: The crystal structure of a light-harvesting protein that interacts with photosystem II is reported.

PDB reference: 2v8a

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1003-1007  [ doi:10.1107/S1744309107054127 ]

Structure of Bacillus subtilis superoxide dismutase

P. Liu, H. E. Ewis, Y.-J. Huang, C.-D. Lu, P. C. Tai and I. T. Weber

Synopsis: The crystal structure of MnSOD with four dimers per asymmetric unit was refined to an R factor of 21.1% at 1.8 Å resolution. Differences in the monomer fold and dimer interface relative to the human enzyme can be exploited in the design of selective drugs for Bacillus MnSOD.

PDB reference: 2rcv

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1008-1013  [ doi:10.1107/S1744309107047781 ]

Hexammineruthenium(III) ion interactions with Z-DNA

D. Bharanidharan, S. Thiyagarajan and N. Gautham

Synopsis: The structure of the complex of the hexanucleotide duplex d(CGCGCA)·d(TGCGCG) with hexammineruthenium(III) ion shows a tautomeric shift in the adenine base and a consequent disruption of the A·T base pair.

PDB references: 2hto and 2htt

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1014-1016  [ doi:10.1107/S1744309107045757 ]

Crystallization and preliminary X-ray diffraction studies of the ISC-like [2Fe-2S] ferredoxin (FdxB) from Pseudomonas putida JCM 20004

T. Iwasaki, D. Ohmori, N. Shimizu and T. Kumasaka

Synopsis: A vertebrate-type [2Fe-2S] ferredoxin (FdxB), which is probably involved in the iron-sulfur cluster-biosynthesis system of the -proteobacterium P. putida JCM 20004, has been crystallized in space group P6₁22. The needle-shaped crystals of recombinant FdxB diffract to 1.90 Å resolution using synchrotron radiation.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1017-1020  [ doi:10.1107/S1744309107049706 ]

Crystallization and preliminary X-ray diffraction analysis on the homing endonuclease I-Dmo-I in complex with its target DNA

P. Redondo, J. Prieto, E. Ramos, F. J. Blanco and G. Montoya

Synopsis: I-Dmo-I is a well characterized homing endonuclease from the archaeon Desulfurococcus mobilis. The enzyme was cloned and overexpressed in Escherichia coli. Crystallization experiments of I-Dmo-I in complex with its DNA target in the presence of Ca²⁺ and Mg²⁺ yielded crystals that were suitable for X-ray diffraction analysis.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1021-1025  [ doi:10.1107/S1744309107051408 ]

The production and crystallization of the human leukocyte antigen class II molecules HLA-DQ2 and HLA-DQ8 complexed with deamidated gliadin peptides implicated in coeliac disease

K. N. Henderson, H. H. Reid, N. A. Borg, S. E. Broughton, T. Huyton, R. P. Anderson, J. McCluskey and J. Rossjohn

Synopsis: The production and crystallization of human leukocyte antigen class II molecules HLA-DQ2 and HLA-DQ8 in complex with deamidated gliadin peptides is reported. Crystals of HLA-DQ2^PQPELPYPQ diffracted to 3.9 Å, while the HLA-DQ8^EGSFQPSQE crystals diffracted to 2.1 Å, allowing structure determination by molecular replacement.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1026-1028  [ doi:10.1107/S1744309107051329 ]

Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal domain of human RSK1 serine/threonine protein kinase

T.-M. Fu, D. Li, J. Nan, L. Li, Y. Xue and X.-D. Su

Synopsis: The C-terminal catalytic domain of serine/threonine kinase RSK1 has been crystallized; the crystals diffracted to a resolution of 2.7 Å and belonged to space group P2₁.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1029-1034  [ doi:10.1107/S1744309107054176 ]

An unexpected outcome of surface engineering an integral membrane protein: improved crystallization of cytochrome ba₃ from Thermus thermophilus

B. Liu, V. M. Luna, Y. Chen, C. D. Stout and J. A. Fee

Synopsis: Mutation of symmetry-related (in space group P4₃2₁2) glutamate and lysine residues on the water-exposed surface of an integral membrane protein, cytochrome ba₃, from T. thermophilus leads to robust crystallization of the protein in space group P4₁2₁2; crystals of these mutant proteins show X-ray diffraction to molecular resolution.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1035-1037  [ doi:10.1107/S1744309107054231 ]

Crystallization and X-ray diffraction analysis of a novel immune-type receptor from Ictalurus punctatus and phasing by selenium anomalous dispersion methods

D. A. Ostrov, J. A. Hernández Prada, R. N. Haire, J. P. Cannon, A. T. Magis, K. Bailey and G. W. Litman

Synopsis: A highly diversified novel immune-type receptor from catfish, NITR10, was crystallized to reveal novel mechanisms of immune recognition.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1038-1040  [ doi:10.1107/S1744309107053973 ]

Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin

T. Nakamura, T. Tonozuka, M. Kotani, K. Obata, K. Oguma and A. Nishikawa

Synopsis: HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1041-1043  [ doi:10.1107/S1744309107054310 ]

Crystallization and initial crystallographic characterization of a vicilin-type seed storage protein from Pinus koraiensis

T. Jin, T.-J. Fu, M. H. Kothary, A. Howard and Y.-Z. Zhang

Synopsis: In this study, the Korean pine (Pinus koraiensis) vicilin-type 7S seed storage protein was isolated from defatted pine-nut extract and purified by sequential gel-filtration and anion-exchange chromatography. Well diffracting single crystals were obtained by the vapour-diffusion method in hanging drops.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1044-1047  [ doi:10.1107/S1744309107054644 ]

Crystallization and preliminary diffraction studies of CBM3b of cellobiohydrolase 9A from Clostridium thermocellum

S. Jindou, S. Petkun, L. Shimon, E. A. Bayer, R. Lamed and F. Frolow

Synopsis: The cloning, expression, purification and crystallization of the CBM3b module of cellobiohydrolase 9A from C. thermocellum is described. The crystals diffract to 2.68 Å.

Online 21 November 2007

 

Acta Cryst. (2007). F63, 1048-1050  [ doi:10.1107/S1744309107054747 ]

Crystallization and preliminary X-ray studies of TON_1713 from Thermococcus onnurineus NA1, a putative member of the haloacid dehalogenase superfamily

B. V. Le, H. S. Lee, Y. Cho, S. G. Kang, D. Y. Kim, Y.-G. Kim and K. K. Kim

Synopsis: A putative member of the haloacid dehalogenase superfamily from T. onnurineus has been expressed, purified and crystallized using 1.6 M magnesium sulfate as a precipitant. The crystals belonged to the triclinic space group P1 and diffracted to 1.8 Å resolution.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1051-1053  [ doi:10.1107/S1744309107055418 ]

Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12

S.-Y. Park, S.-H. Lee, J. Lee, C.-H. Jung and J.-S. Kim

Synopsis: The crystallization of ybfF, a new esterase from E. coli, and the collection of diffraction data to 1.1 Å resolution are reported.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1054-1057  [ doi:10.1107/S1744309107055571 ]

Expression, purification and crystallization of human 5-lipoxygenase-activating protein with leukotriene-biosynthesis inhibitors

S. Xu, B. M. McKeever, D. Wisniewski, D. K. Miller, R. H. Spencer, L. Chu, F. Ujjainwalla, T.-T. Yamin, J. F. Evans, J. W. Becker and A. D. Ferguson

Synopsis: The expression, purification and crystallization of human 5-lipoxygenase-activating protein in complex with two leukotriene-biosynthesis inhibitors is decribed. The processes that were used to generate diffraction quality crystals are presented in detail.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1058-1060  [ doi:10.1107/S1744309107056953 ]

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of ScpB (Rv1710) from Mycobacterium tuberculosis

S.-Y. Kwon, B. S. Kang, M. H. Kim and K. J. Kim

Synopsis: ScpB from M. tuberculosis was crystallized using the sitting-drop vapour-diffusion method in the presence of 2 M NaCl and 10% PEG 6000 at 295 K. X-ray diffraction data were collected to a maximum resolution of 2.3 Å at a synchrotron beamline.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1061-1063  [ doi:10.1107/S1744309107057466 ]

Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex

K. Nishida, M. Horiuchi, N. N. Noda, K. Takahasi, N. Iwasaki, A. Minami and F. Inagaki

Synopsis: The antiproliferative complex Tob-hCaf1 was purified and crystallized using the sitting-drop vapour-diffusion method. The crystal diffracted to around 2.6 Å resolution.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1064-1066  [ doi:10.1107/S1744309107057636 ]

Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12

J.-W. Choi, J. Lee, N. Kosuke, C.-H. Jung and J.-S. Kim

Synopsis: Crystals scattering to 2.00 Å resolution have been obtained of the ydjA nitroreductase from E. coli K12.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1067-1069  [ doi:10.1107/S1744309107058563 ]

Preliminary X-ray crystallographic studies of a tetrameric phospholipase A₂ formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom

D. P. Marchi-Salvador, L. C. Corrêa, G. H. M. Salvador, A. J. Magro, C. Z. Oliveira, J. Iulek, A. M. Soares and M. R. M. Fontes

Synopsis: Crotoxin B is a basic phospholipase A₂ found in the venom of C. durissus terrificus and is one of the subunits that constitute crotoxin. Here, the crystallization, X-ray diffraction data collection and molecular-replacement solution of a novel tetrameric complex formed by two dimers of crotoxin B isoforms are presented.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1070-1072  [ doi:10.1107/S1744309107059672 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the plant Rho protein ROP5

C. Thomas and A. Berken

Synopsis: Crystals of the plant Rho protein ROP5 from A. thaliana have been obtained that diffract to 1.53 Å resolution.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1073-1076  [ doi:10.1107/S1744309107060289 ]

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7

O. Quesada, B. Gurda, L. Govindasamy, R. McKenna, E. Kohlbrenner, G. Aslanidi, S. Zolotukhin, N. Muzyczka and M. Agbandje-McKenna

Synopsis: Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids have been produced which diffract X-rays to 3.0 Å resolution.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1077-1079  [ doi:10.1107/S1744309107060721 ]

Crystallization and preliminary X-ray diffraction analysis of the small laccase from Streptomyces coelicolor

T. Skálová, J. Dohnálek, L. H. Østergaard, P. R. Østergaard, P. Kolenko, J. Dusková and J. Hasek

Synopsis: The expression, purification and crystallization of the small laccase from S. coelicolor are reported. Diffraction data were collected to 3 Å resolution.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1080-1083  [ doi:10.1107/S1744309107061283 ]

Preliminary neutron and ultrahigh-resolution X-ray diffraction studies of the aspartic proteinase endothiapepsin cocrystallized with a gem-diol inhibitor

H.-F. Tuan, P. Erskine, P. Langan, J. Cooper and L. Coates

Synopsis: Three data sets have been collected on endothiapepsin complexed with the gem-diol inhibitor PD-135,040: a high-resolution synchrotron X-ray data set, a room-temperature X-ray data set and a neutron diffraction data set. Until recently, it has been impossible to grow large protein crystals of endothiapepsin with any gem-diol inhibitor that are suitable for neutron diffraction.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1084-1086  [ doi:10.1107/S1744309107061118 ]

Purification, crystallization and preliminary structural analysis of nucleoside diphosphate kinase from Bacillus anthracis

G. Misra, A. Aggarwal, S. Mittal, Y. Singh and R. Ramachandran

Synopsis: Nucleoside diphosphate kinase from B. anthracis has been crystallized. Preliminary crystallographic analysis shows that there is one monomer in the asymmetric unit of the crystal.

Online 30 November 2007

 

Acta Cryst. (2007). F63, 1087-1090  [ doi:10.1107/S1744309107056424 ]

Crystallization, data collection and processing of the chymotrypsin-BTCI-trypsin ternary complex

G. F. Esteves, R. C. L. Teles, N. S. Cavalcante, D. Neves, M. M. Ventura, J. A. R. G. Barbosa and S. M. de Freitas

Synopsis: A ternary complex of the proteinase inhibitor (BTCI) with trypsin and chymotrypsin was crystallized and its crystal structure was solved by molecular replacement.

Online 30 November 2007