Structural Biology and Crystallization Communications

 

Acta Cryst. (2008). F64, 2-7  [ doi:10.1107/S1744309107065931 ]

Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis

N. Lack, E. D. Lowe, J. Liu, L. D. Eltis, M. E. M. Noble, E. Sim and I. M. Westwood

Synopsis: The structure of HsaD, a carbon-carbon bond serine hydrolase involved in steroid catabolism that is critical for the survival of M. tuberculosis inside human macrophages, has been solved by X-ray crystallography. Data were collected at the Diamond Light Source in Oxfordshire, England: this paper describes one of the first structures determined at the new synchrotron.

PDB reference: 2vf2

Online 20 December 2007

 

Acta Cryst. (2008). F64, 8-13  [ doi:10.1107/S1744309107066481 ]

The structure of melon necrotic spot virus determined at 2.8 Å resolution

Y. Wada, H. Tanaka, E. Yamashita, C. Kubo, T. Ichiki-Uehara, E. Nakazono-Nagaoka, T. Omura and T. Tsukihara

Synopsis: The structure of melon necrotic spot virus is reported.

PDB reference: 2zah

Online 20 December 2007

 

Acta Cryst. (2008). F64, 14-18  [ doi:10.1107/S1744309107057260 ]

Semi-automated microseeding of nanolitre crystallization experiments

T. S. Walter, E. J. Mancini, J. Kadlec, S. C. Graham, R. Assenberg, J. Ren, S. Sainsbury, R. J. Owens, D. I. Stuart, J. M. Grimes and K. Harlos

Synopsis: A procedure for microseeding into nanolitre crystallization drops is described with selected successful examples.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 19-21  [ doi:10.1107/S1744309107059696 ]

Crystallization and preliminary X-ray studies of SdiA from Escherichia coli

C. Wu, N. K. Lokanath, D. Y. Kim, L. D. N. Nguyen and K. K. Kim

Synopsis: E. coli SdiA was overexpressed, purified and crystallized. The crystals belonged to the hexagonal space group P6₁22 or P6₅22 and diffracted to 2.7 Å resolution.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 22-24  [ doi:10.1107/S1744309107060897 ]

Overexpression, purification and crystallization of the tetrameric form of SorC sorbitol operon regulator

D. de Sanctis, A. T. Rêgo, D. Marçal, C. E. McVey, M. A. Carrondo and F. J. Enguita

Synopsis: The sorbitol operon regulator from K. pneumoniae has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 3.2 Å.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 25-28  [ doi:10.1107/S174430910706160X ]

Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine -lactoglobulin allergen

M. Niemi, J. Jänis, S. Jylhä, J. M. Kallio, N. Hakulinen, M.-L. Laukkanen, K. Takkinen and J. Rouvinen

Synopsis: The high-resolution mass-spectrometric characterization, crystallization and X-ray diffraction studies of a recombinant IgE Fab fragment in complex with bovine -lactoglobulin are reported.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 29-31  [ doi:10.1107/S1744309107064391 ]

Overproduction, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima

C. Barbey, N. Rouhier, A. Haouz, A. Navaza and J.-P. Jacquot

Synopsis: Crystals of the peroxiredoxin domain of a larger natural hybrid protein from T. maritima were obtained which diffracted to 2.9 Å resolution on a synchrotron source.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 32-35  [ doi:10.1107/S1744309107064615 ]

Purification, identification and preliminary crystallographic studies of Pru du amandin, an allergenic protein from Prunus dulcis

V. Gaur, D. K. Sethi and D. M. Salunke

Synopsis: The purification, identification, crystallization and preliminary crystallographic studies of an allergy-related protein, Pru du amandin, from P. dulcis nuts are reported.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 36-38  [ doi:10.1107/S1744309107065256 ]

Overproduction, crystallization and preliminary X-ray analysis of the putative L-ascorbate-6-phosphate lactonase UlaG from Escherichia coli

F. Garces, F. J. Fernández, R. Pérez-Luque, J. Aguilar, L. Baldomà, M. Coll, J. Badía and M. C. Vega

Synopsis: UlaG, the putative L-ascorbate-6-phosphate lactonase encoded by the ulaG gene from the utilization of L-ascorbate regulon in E. coli, has been cloned, overexpressed, purified using standard chromatographic techniques and crystallized in a monoclinic space group. Crystals were obtained by the sitting-drop vapour-diffusion method at 293 K. A data set diffracting to 3 Å resolution was collected from a single crystal at 100 K.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 39-43  [ doi:10.1107/S1744309107065372 ]

Crystallization and preliminary crystallographic analysis of the catalytic domain of human flap endonuclease 1 in complex with a nicked DNA product: use of a DPCS kit for efficient protein-DNA complex crystallization

S. Sakurai, K. Kitano, H. Morioka and T. Hakoshima

Synopsis: Human flap endonuclease 1 complexed with nicked DNA has been crystallized. A diffraction data set was collected to a resolution of 2.75 Å.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 44-46  [ doi:10.1107/S1744309107065384 ]

Expression, crystallization and preliminary X-ray diffraction analysis of human paired Ig-like type 2 receptor (PILR)

S. Tabata, K. Kuroki, N. Maita, J. Wang, I. Shiratori, H. Arase, D. Kohda and K. Maenaka

Synopsis: Human paired Ig-like type 2 receptor (PILR) has been expressed, purified and crystallized. A diffraction data set has been collected to 1.3 Å resolution.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 47-49  [ doi:10.1107/S1744309107065645 ]

Preliminary X-ray crystallographic analysis of SMU.573, a putative sugar kinase from Streptococcus mutans

Y.-F. Zhou, L.-F. Li, C. Yang, Y.-H. Liang and X.-D. Su

Synopsis: SMU.573 from S. mutans was expressed in E. coli and crystallized. The crystals belong to space group I4 and 2.5 Å resolution diffraction data were collected at an in-house chromium radiation source.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 50-53  [ doi:10.1107/S1744309107066122 ]

Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114

S. Martínez-Rodríguez, L. A. González-Ramírez, J. M. Clemente-Jiménez, F. Rodríguez-Vico, F. J. Las Heras-Vázquez, J. A. Gavira and J. M. García-Ruiz

Synopsis: Crystals of an active-site mutated hydantoin racemase from S. meliloti have been obtained in the presence and absence of D,L-5-isopropyl-hydantoin and characterized by X-ray diffraction.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 54-57  [ doi:10.1107/S1744309107066444 ]

Cleaved thioredoxin fusion protein enables the crystallization of poorly soluble ER in complex with synthetic ligands

V. Cura, M. Gangloff, S. Eiler, D. Moras and M. Ruff

Synopsis: A new crystallization strategy: the presence of cleaved thioredoxin fusion is critical for crystallization of the estrogen nuclear receptor ligand binding domain in complex with synthetic ligands. This novel technique should be regarded as an interesting alternative for crystallization of difficult proteins.

Online 20 December 2007

 

Acta Cryst. (2008). F64, 58-61  [ doi:10.1107/S1744309107066821 ]

Crystallization and preliminary X-ray analysis of Acetivibrio cellulolyticus cellulosomal type II cohesin module: two versions having different linker lengths

I. Noach, O. Alber, E. A. Bayer, R. Lamed, M. Levy-Assaraf, L. J. W. Shimon and F. Frolow

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray characterization of two protein constructs of the second type II cohesin module from A. cellulolyticus ScaB are described. Both constructs contain the native N-terminal linker, but only one of them contains the full-length 45-residue C-terminal linker; the other contains a five-residue segment of this linker.

Online 20 December 2007