Structural Biology and Crystallization Communications

 

Acta Cryst. (2008). F64, 64-69  [ doi:10.1107/S174430910706770X ]

Structural basis for the binding of naproxen to human serum albumin in the presence of fatty acids and the GA module

S. Lejon, J. F. Cramer and P. Nordberg

Synopsis: The crystal structure of the complex formed by human serum albumin and a bacterial albumin-binding domain with the drug compound naproxen and fatty acid decanoate bound to albumin is described.

PDB reference: 2vdb

Online 18 January 2008

 

Acta Cryst. (2008). F64, 70-76  [ doi:10.1107/S1744309107068236 ]

Structure of calcium-bound human S100A13 at pH 7.5 at 1.8 Å resolution

F. L. Imai, K. Nagata, N. Yonezawa, M. Nakano and M. Tanokura

Synopsis: The crystal structure of Ca²⁺-bound human S100A13 at pH 7.5 is reported.

PDB reference: 2egd

Online 31 January 2008

 

Acta Cryst. (2008). F64, 77-80  [ doi:10.1107/S1744309107067437 ]

Preliminary X-ray characterization of a novel type of anchoring cohesin from the cellulosome of Ruminococcus flavefaciens

O. Alber, I. Noach, R. Lamed, L. J. W. Shimon, E. A. Bayer and F. Frolow

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray characterization of a novel class of cohesin module (type III) from the R. flavefaciens ScaE anchoring scaffoldin are described.

Online 18 January 2008

 

Acta Cryst. (2008). F64, 81-84  [ doi:10.1107/S1744309107067930 ]

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of the mannose 6-phosphate isomerase from Salmonella typhimurium

G. Gowda, S. R. Sagurthi, H. S. Savithri and M. R. N. Murthy

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray crystallographic studies of mannose 6-phosphate isomerase from S. typhimurium are reported.

Online 18 January 2008

 

Acta Cryst. (2008). F64, 85-87  [ doi:10.1107/S174430910706825X ]

Crystallization and preliminary X-ray diffraction analysis of a glutathione S-transferase from Xylella fastidiosa

W. Garcia, R. F. Travensolo, N. C. Rodrigues, J. R. C. Muniz, C. S. Caruso, E. G. M. Lemos, A. P. U. Araujo and E. Carrilho

Synopsis: Glutathione S-transferase from X. fastidiosa (xfGST) has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.23 Å.

Online 18 January 2008

 

Acta Cryst. (2008). F64, 88-90  [ doi:10.1107/S1744309107068522 ]

Purification, crystallization and preliminary crystallographic study of a recombinant plant aminoaldehyde dehydrogenase from Pisum sativum

M. Tylichová, P. Briozzo, D. Kopecný, J. Ferrero, S. Moréra, N. Joly, J. Snégaroff and M. Sebela

Synopsis: A 2.8 Å resolution data set was collected from a crystal of a recombinant plant aminoaldehyde dehydrogenase from P. sativum. This enzyme oxidizes -aminoaldehydes arising from polyamine degradation.

Online 18 January 2008

 

Acta Cryst. (2008). F64, 91-93  [ doi:10.1107/S1744309107068649 ]

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304

P. Bahti, S. Chen, Y. Li, N. Shaw, X. Zhang, M. Zhang, C. Cheng, G. Song, J. Yin, H. Zhang, D. Che, A. Abbas, H. Xu, B.-C. Wang and Z.-J. Liu

Synopsis: A non-Pfam protein, AF1514, from A. fulgidus has been crystallized.

Online 18 January 2008

 

Acta Cryst. (2008). F64, 94-97  [ doi:10.1107/S1744309108000055 ]

Crystallization and preliminary diffraction analysis of a DsbA homologue from Wolbachia pipientis

M. Kurz, I. Iturbe-Ormaetxe, R. Jarrott, S. L. O'Neill, K. A. Byriel, J. L. Martin and B. Heras

Synopsis: The first crystallization of a W. pipientis protein, -DsbA1, was achieved using hanging-drop and sitting-drop vapour diffusion.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 98-101  [ doi:10.1107/S1744309108000328 ]

Purification, crystallization and preliminary X-ray analysis of inositol dehydrogenase (IDH) from Bacillus subtilis

K. E. Van Straaten, A. Hoffort, D. R. J. Palmer and D. A. R. Sanders

Synopsis: Selenomethionine-substituted IDH was crystallized using the microbatch method. The crystals diffracted to beyond 2.0 Å resolution using synchrotron radiation.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 102-104  [ doi:10.1107/S1744309108000341 ]

Crystallization and preliminary crystallographic studies of L30e, a ribosomal protein from Methanocaldococcus jannaschii (MJ1044)

S. Rangarajan, J. Jeyakanthan, P. Mridula, K. Sakamoto, Y. Kitamura, Y. Agari, A. Shinkai, A. Ebihara, S. Kuramitsu, S. Yokoyama and K. Sekar

Synopsis: The ribosomal protein (L30e) from M. jannaschii was cloned from the gene MJ1044, expressed, purified and crystallized. The crystal belongs to the primitive tetragonal space group P4₃ and diffracted to 1.9 Å resolution.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 105-107  [ doi:10.1107/S1744309108000626 ]

Purification, crystallization and preliminary crystallographic analysis of a 6-pyruvoyltetrahydropterin synthase homologue from Esherichia coli

K. H. Seo, Supangat, H. L. Kim, Y. S. Park, C. O. Jeon and K. H. Lee

Synopsis: The 6-pyruvoyltetrahydropterin synthase from E. coli has been crystallized in two crystal forms. Diffraction data were collected from hexagonal- and rectangular-shaped crystals to 3.0 and 2.3 Å, respectively.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 108-110  [ doi:10.1107/S1744309108000833 ]

Preliminary X-ray crystallographic studies of mouse UPR responsive protein P58(IPK) TPR fragment

J. Tao, Y. Wu, D. Ron and B. Sha

Synopsis: To investigate the mechanism by which P58(IPK) functions to promote protein folding within the ER, a P58(IPK) TPR fragment without the C-terminal J-domain has been crystallized.

Online 31 January 2008

    

Acta Cryst. (2008). F64, 111-114  [ doi:10.1107/S1744309108000754 ]

Preliminary crystallographic data of the three homologues of the thiol-disulfide oxidoreductase DsbA in Neisseria meningitidis

C. Lafaye, T. Iwena, J.-L. Ferrer, J. S. Kroll, M. Griat and L. Serre

Synopsis: The Neisseria meningitidis genome possesses three genes encoding active DsbAs. To throw light on the reason for this genetic multiplicity, the three enzymes have been purified and crystallized.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 115-118  [ doi:10.1107/S1744309108001000 ]

Overproduction, purification and preliminary crystallographic analysis of the carbohydrate-recognition domain of human langerin

M. Thépaut, C. Vivès, G. Pompidor, R. Kahn and F. Fieschi

Synopsis: Crystals of the carbohydrate-recognition domain of human langerin were obtained that diffracted synchrotron radiation to 1.5 Å resolution.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 119-122  [ doi:10.1107/S1744309108000924 ]

Crystallization and preliminary X-ray characterization of queD from Bacillus subtilis, an enzyme involved in queuosine biosynthesis

N. Cicmil and L. Shi

Synopsis: B. subtilis queD was overexpressed, purified and crystallized. A diffraction data set was collected to a resolution of 3.6 Å.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 123-125  [ doi:10.1107/S1744309108001206 ]

Crystallization of the extracellular rubber oxygenase RoxA from Xanthomonas sp. strain 35Y

M. Hoffmann, R. Braaz, D. Jendrossek and O. Einsle

Synopsis: The extracellular rubber-degrading enzyme rubber oxygenase A (RoxA) from Xanthomonas sp. strain 35Y has been crystallized and diffraction data have been collected to high resolution.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 126-129  [ doi:10.1107/S1744309108001450 ]

Purification, crystallization and preliminary X-ray analysis of aminoglycoside-2''-phosphotransferase-Ic [APH(2'')-Ic] from Enterococcus gallinarum

L. J. Byrnes, A. Badarau, S. B. Vakulenko and C. A. Smith

Synopsis: APH(2'')-Ic is an enzyme that is responsible for high-level gentamicin resistance in E. gallinarum isolates. Crystals of the wild-type enzyme and three mutants have been prepared and a complete X-ray diffraction data set was collected to 2.15 Å resolution from an F108L crystal.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 130-132  [ doi:10.1107/S1744309108001760 ]

Crystallization and preliminary X-ray crystallographic analyses of Nur, a nickel-responsive transcription regulator from Streptomyces coelicolor

Y. J. An, B.-E. Ahn, J.-H. Roe and S.-S. Cha

Synopsis: A nickel uptake regulator (Nur) from Streptomyces coelicolor has been crystallized. A 2.4 Å native data set and a 3.0 Å Ni-MAD data set were collected using synchrotron radiation.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 133-136  [ doi:10.1107/S1744309108001905 ]

Crystallization and preliminary crystallographic analysis of human Ca²⁺-loaded calbindin-D28k

C. Zhang, Y. Sun, W. Wang, Y. Zhang, M. Ma and Z. Lou

Synopsis: Human calbindin-D28k has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.4 Å resolution.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 137-140  [ doi:10.1107/S1744309108002066 ]

Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis

A. Stsiapanava, T. Koudelakova, M. Lapkouski, M. Pavlova, J. Damborsky and I. Kuta Smatanova

Synopsis: Three mutants of the haloalkane dehalogenase DhaA derived from R. rhodochrous NCIMB 13064 were crystallized and diffracted to ultrahigh resolution.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 141-143  [ doi:10.1107/S1744309108002273 ]

Purification, crystallization and preliminary crystallographic analysis of Streptococcus pyogenes laminin-binding protein Lbp

C. Linke, T. T. Caradoc-Davies, T. Proft and E. N. Baker

Synopsis: The S. pyogenes laminin-binding protein Lbp, which is essential for adhesion to human laminin, has been expressed, purified and crystallized.

Online 31 January 2008

 

Acta Cryst. (2008). F64, 144-148  [ doi:10.1107/S1744309108002364 ]

Purification, crystallization and preliminary structural characterization of the periplasmic domain P1 of the Escherichia coli membrane-protein insertase YidC

S. Ravaud, K. Wild and I. Sinning

Synopsis: The overproduction, purification, crystallization and preliminary crystallographic studies of the native and selenomethionine-labelled P1 domain are reported here as a first step towards the elucidation of the molecular mechanism of YidC as a membrane-protein insertase.

Online 31 January 2008