Acta Cryst. (2008). F64, 154-155
[ doi:10.1107/S1744309108005617 ]

A new service for preparing enhanced figures in IUCr journals

H. Einspahr and M. Guss

Online 29 February 2008

 

Acta Cryst. (2008). F64, 156-162
[ doi:10.1107/S1744309108001413 ]

Structure of mouse ADP-ribosylhydrolase 3 (mARH3)

C. Mueller-Dieckmann, S. Kernstock, J. Mueller-Dieckmann, M. S. Weiss and F. Koch-Nolte

Synopsis: The crystal structure of ADP-ribosylhydrolase 3 from M. musculus has been determined and refined to a resolution of 1.8 Å. A detailed comparison with the human orthologue at the protein-sequence level as well as of the three-dimensional architecture is presented.

PDB reference: 2qty

Online 23 February 2008

 

Acta Cryst. (2008). F64, 163-166
[ doi:10.1107/S1744309108002078 ]

A novel acetate-bound complex of human carbonic anhydrase II

P. A. Mazumdar, D. Kumaran, S. Swaminathan and A. K. Das

Synopsis: A complex of human carbonic anhydrase II with its inhibitor acetate is described where the ligand is found to bind in an orientation different from that previously described.

PDB reference: 1xeg

Online 23 February 2008

 

Acta Cryst. (2008). F64, 167-170
[ doi:10.1107/S1744309108002753 ]

Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis

R. Vyas, V. Kumar, S. Panjikar, S. Karthikeyan, K. V. R. Kishan, R. Tewari and M. S. Weiss

Synopsis: The enzyme aspartate semialdehyde dehydrogenase from M. tuberculosis has been expressed, purified and crystallized in two different crystal forms.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 171-174
[ doi:10.1107/S1744309108002662 ]

Preparation, crystallization and preliminary X-ray diffraction analysis of the DNA-binding domain of the Ets transcription factor in complex with target DNA

Y. Suwa, T. Nakamura, S. Toma, S. Ikemizu, H. Kai and Y. Yamagata

Synopsis: The complex between the Ets domain of Ets2 and its target DNA has been crystallized. The crystals diffracted to 3.0 Å resolution.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 175-178
[ doi:10.1107/S1744309108003370 ]

Crystallization and preliminary X-ray analysis of human Brn-5 transcription factor in complex with DNA

J. H. Pereira, S. C. Ha and S.-H. Kim

Synopsis: The human Brn-5 transcription factor has been crystallized in complex with DNA. Diffraction data were collected to 2.80 Å.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 179-181
[ doi:10.1107/S1744309108003473 ]

Crystallization and preliminary X-ray diffraction analysis of full-length and proteolytically activated pyruvate oxidase from Escherichia coli

A. Weidner, P. Neumann, G. Wille, M. T. Stubbs and K. Tittmann

Synopsis: The peripheral membrane flavoprotein pyruvate oxidase from E. coli has been crystallized in the full-length form and as a proteolytically activated truncation variant lacking the last 23 amino acids at the C-terminus.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 182-185
[ doi:10.1107/S174430910800345X ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of variants of monoamine oxidase from Aspergillus niger

K. E. Atkin, R. Reiss, N. J. Turner, A. M. Brzozowski and G. Grogan

Synopsis: Crystals of A. niger monoamine oxidase variants display P2₁ or P4₁2₁2/P4₃2₁2 symmetry, with eight or two molecules in the asymmetric unit, respectively.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 186-189
[ doi:10.1107/S1744309108003667 ]

Crystallization and preliminary X-ray studies of ferredoxin-NAD(P)⁺ reductase from Chlorobium tepidum

N. Muraki, D. Seo, T. Shiba, T. Sakurai and G. Kurisu

Synopsis: Ferredoxin-NAD(P)⁺ reductase from C. tepidum has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.4 Å resolution.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 190-192
[ doi:10.1107/S1744309108003680 ]

Crystallization and preliminary X-ray diffraction analysis of vaccinia virus H1L phosphatase

L. Roces, P. P. Knowles, G. Fox, J. Juanhuix, N. Scaplehorn, M. Way and N. Q. McDonald

Synopsis: A catalytically inactive mutant of the dual-specificity phosphatase H1L from vaccinia virus was expressed recombinantly, purified and crystallized by the microbatch method. The crystals belong to the tetragonal space group P422 and diffraction data were collected to 2.1 Å resolution using a synchrotron-radiation source. Attempts to derivatize these crystals with xenon gas lead to a space-group change to I422 with a smaller unit cell and a diffraction limit of 3.0 Å.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 193-195
[ doi:10.1107/S1744309108003825 ]

Crystallization and preliminary X-ray crystallographic characterization of TrmFO, a folate-dependent tRNA methyltransferase from Thermotoga maritima

N. Cicmil

Synopsis: T. maritima TrmFO was overexpressed, purified and crystallized. A diffraction data set was collected to a resolution of 2.6 Å.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 196-199
[ doi:10.1107/S1744309108003849 ]

Crystallization and preliminary X-ray analysis of RsbS from Moorella thermoacetica at 2.5 Å resolution

M. Quin, J. Newman, S. Firbank, R. J. Lewis and J. Marles-Wright

Synopsis: Crystallization and selenium substructure solution of RsbS from Moorella thermoacetica, the first ab initio phased crystal structure from Diamond.

Online 23 February 2008

 

Acta Cryst. (2008). F64, 200-202
[ doi:10.1107/S1744309107068388 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of grass carp ₂-microglobulin

W. Chen, F. Chu, H. Peng, J. Zhang, J. Qi, F. Jiang, C. Xia and F. Gao

Synopsis: Grass carp ₂-microglobulin was expressed in E. coli, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method with PEG 2K as precipitant. The crystals obtained belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 38.72, b = 40.65, c = 71.12 Å.

Online 29 February 2008

 

Acta Cryst. (2008). F64, 203-205
[ doi:10.1107/S1744309108002510 ]

Purification, crystallization and preliminary X-ray analysis of the peptidoglycan N-acetylglucosamine deacetylase BC1960 from Bacillus cereus in the presence of its substrate (GlcNAc)₆

A. Tsalafouta, E. Psylinakis, E. G. Kapetaniou, D. Kotsifaki, A. Deli, A. Roidis, V. Bouriotis and M. Kokkinidis

Synopsis: The peptidoglycan N-acetylglucosamine (GlcNAc) deacetylase BC1960 from B. cereus was crystallized in the presence of the substrate (GlcNAc)₆. The crystals belonged to space group P4₁2₁2 and diffracted to 2.38 Å resolution.

Online 29 February 2008

 

Acta Cryst. (2008). F64, 206-208
[ doi:10.1107/S1744309108002819 ]

The purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Clostridium botulinum

R. C. J. Dobson, S. C. Atkinson, M. A. Gorman, J. M. Newman, M. W. Parker and M. A. Perugini

Synopsis: Dihydrodipicolinate synthase (DHDPS), an enzyme in the lysine-biosynthetic pathway, is a promising target for antibiotic development against pathogenic bacteria. Here, the expression, purification, crystallization and preliminary diffraction analysis of DHDPS from C. botulinum are reported.

Online 29 February 2008

 

Acta Cryst. (2008). F64, 209-212
[ doi:10.1107/S1744309108002832 ]

Crystallization and preliminary X-ray study of native and selenomethionyl -1,4-mannanase AaManA from Alicyclobacillus acidocaldarius Tc-12-31

Y. Zhang, F. Gao, Y. Xue, Y. Zeng, H. Peng, J. Qi and Y. Ma

Synopsis: A. acidocaldarius Tc-12-31 -1,4-mannanase was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic studies were performed for both the native mannanase and its selenomethionyl derivative.

Online 29 February 2008

 

Acta Cryst. (2008). F64, 213-216
[ doi:10.1107/S1744309108003096 ]

Expression, purification, crystallization and preliminary X-ray characterization of two crystal forms of stationary-phase survival E protein from Campylobacter jejuni

A. M. D. Gonçalves, A. T. Rêgo, M. Thomaz, F. J. Enguita and M. A. Carrondo

Synopsis: Survival E (SurE) protein from Campylobacter jejuni, a Gram-negative mesophile, has been overexpressed in Escherichia coli as a soluble protein, successfully purified and crystallized in two distinct crystal forms.

Online 29 February 2008

 

Acta Cryst. (2008). F64, 217-220
[ doi:10.1107/S1744309108003795 ]

Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa

C. Taguchi, F. Taura, T. Tamada, Y. Shoyama, Y. Shoyama, H. Tanaka, R. Kuroki and S. Morimoto

Synopsis: Polyketide synthase-1 from C. sativa has been crystallized. The crystal diffracted to 1.55 Å resolution with sufficient quality for further structure determination.

Online 29 February 2008

 

Acta Cryst. (2008). F64, 221-223
[ doi:10.1107/S1744309108004211 ]

Overproduction, purification and crystallization of a chondroitin sulfate A-binding DBL domain from a Plasmodium falciparum var2csa-encoded PfEMP1 protein

M. K. Higgins

Synopsis: A chondroitin sulfate A-binding DBL important in placental malaria has been overproduced, purified and crystallized. Diffraction data were collected to 1.9 Å resolution.

Online 29 February 2008

 

Acta Cryst. (2008). F64, 224-227
[ doi:10.1107/S1744309108004387 ]

Expression, purification, crystallization and preliminary X-ray analysis of the polysaccharide lyase RB5312 from the marine planctomycete Rhodopirellula baltica

J. Dabin, M. Jam, M. Czjzek and G. Michel

Synopsis: This study describes the crystallization and preliminary X-ray analysis of the family PL1 polysaccharide lyase RB5312 from the marine bacterium R. baltica. Purified recombinant protein was crystallized; the crystals belonged to space group P2₁2₁2₁ and diffracted X-rays to a resolution of 1.8 Å.

Online 29 February 2008

 

Acta Cryst. (2008). F64, 228-230
[ doi:10.1107/S1744309108004326 ]

Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-dehydrogenase

Y. Asada, C. Kuroishi, Y. Ukita, R. Sumii, S. Endo, T. Matsunaga, A. Hara and N. Kunishima

Synopsis: The preliminary X-ray crystallographic study of rabbit L-gulonate 3-dehydrogenase is described.

Online 29 February 2008