Structural Biology and Crystallization Communications

 

Acta Cryst. (2008). F64, 233-238  [ doi:10.1107/S1744309108004995 ]

Structural analysis of the complex of Keap1 with a prothymosin peptide

B. Padmanabhan, Y. Nakamura and S. Yokoyama

Synopsis: The crystal structure of the complex of mouse Keap1-DC with a fragment of the nuclear protein prothymosin was determined and refined to 1.9 Å resolution and revealed that the peptide binds to the bottom region of the -propeller domain of Keap1-DC.

PDB reference: 2z32

Online 21 March 2008

 

Acta Cryst. (2008). F64, 239-242  [ doi:10.1107/S174430910800568X ]

Monoclinic form of isopentenyl diphosphate isomerase: a case of polymorphism in biomolecular crystals

J. de Ruyck, Y. Oudjama and J. Wouters

Synopsis: Type 1 isopentenyl diphosphate isomerase (IDI-1) in a new crystal form.

PDB references: 2vnq and 2vnp

Online 21 March 2008

 

Acta Cryst. (2008). F64, 243-246  [ doi:10.1107/S1744309108007574 ]

Structure of the SH3 domain of rat endophilin A2

P. J. Loll, E. Swain, Y. Chen, B. T. Turner and J. Zhang

Synopsis: The crystal structure of the SH3 domain of rat endophilin A2 has been determined by the multiwavelength anomalous dispersion method and refined at a resolution of 1.70 Å to R and R_free values of 0.196 and 0.217, respectively.

PDB reference: 3c0c

Online 29 March 2008

 

Acta Cryst. (2008). F64, 247-251  [ doi:10.1107/S1744309108005411 ]

Structure of the cold-shock domain protein from Neisseria meningitidis reveals a strand-exchanged dimer

J. Ren, J. E. Nettleship, S. Sainsbury, N. J. Saunders and R. J. Owens

Synopsis: The X-ray crystal structure of the cold-shock domain protein from N. meningitidis reveals a strand-exchanged dimer.

PDB reference: 3cam

Online 21 March 2008

 

Acta Cryst. (2008). F64, 252-254  [ doi:10.1107/S1744309108004132 ]

Crystallization and preliminary X-ray crystallographic analysis of a carbonyl reductase from Candida parapsilosis

R. Zhang, Y. Xu, Y. Sun, Y. Nie, X. Mu, X. Li, X. C. Zhang and Z. Rao

Synopsis: A novel short-chain NADPH-dependent (S)-1-phenyl-1,2-ethanediol dehydrogenase (SCR) has been crystallized.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 255-257  [ doi:10.1107/S1744309108004594 ]

Crystallization and preliminary X-ray diffraction analysis of the glucuronoyl esterase catalytic domain from Hypocrea jecorina

S. J. Wood, X.-L. Li, M. A. Cotta, P. Biely, N. E. C. Duke, M. Schiffer and P. R. Pokkuluri

Synopsis: The catalytic domain of the glucuronoyl esterase from H. jecorina was overexpresssed, purified and crystallized in space group P2₁2₁2₁. X-ray diffraction data were collected to 1.9 Å resolution.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 258-262  [ doi:10.1107/S1744309108004557 ]

Expression, purification and crystallization of a lyssavirus matrix (M) protein

R. Assenberg, O. Delmas, S. C. Graham, A. Verma, N. Berrow, D. I. Stuart, R. J. Owens, H. Bourhy and J. M. Grimes

Synopsis: The expression, purification and crystallization of the full-length matrix protein from three lyssaviruses is described.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 263-265  [ doi:10.1107/S1744309108004569 ]

Structural analysis of bacteriophage-encoded peptidoglycan hydrolase domain KMV36C: crystallization and preliminary X-ray diffraction

K. Van Hecke, Y. Briers, R. Derua, E. Waelkens, R. Lavigne and L. Van Meervelt

Synopsis: Crystallization and X-ray data collection of the C-terminus of gp36 from bacteriophage KMV (KMV36C) are reported.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 266-269  [ doi:10.1107/S1744309108004752 ]

Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to the extracellular IL-22R1 chain

B. C. Jones, N. J. Logsdon and M. R. Walter

Synopsis: Crystals of the IL-22-sIL-22R1 complex have been obtained by hanging-drop vapor-diffusion experiments combined with streak-seeding at 298 K. A complete low-temperature 3.2 Å resolution data set has been collected using synchrotron radiation.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 270-273  [ doi:10.1107/S1744309108005137 ]

Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin

A. Y. Kovalevsky, T. Chatake, N. Shibayama, S.-Y. Park, T. Ishikawa, M. Mustyakimov, S. Z. Fisher, P. Langan and Y. Morimoto

Synopsis: In order to investigate the role of the protonation states of protein residues in O₂ binding, large crystals of deoxy HbA (20 mm³) were grown in D₂O under anaerobic conditions for neutron diffraction studies.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 274-276  [ doi:10.1107/S1744309108005186 ]

Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis

H. Y. Cho, H. J. Cho, Y. M. Kim, J. I. Oh and B. S. Kang

Synopsis: The second GAF domain of the hypoxia-sensing DevS from M. smegmatis has been crystallized. The crystal belonged to space group P6₁ and diffracted to 2.0 Å resolution.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 277-280  [ doi:10.1107/S1744309108005277 ]

Cloning, purification and preliminary X-ray analysis of the C-terminal domain of Helicobacter pylori MotB

A. Roujeinikova

Synopsis: The cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of a putative peptidoglycan-binding domain of H. pylori MotB, a stator component of the bacterial flagellar motor, are reported.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 281-283  [ doi:10.1107/S1744309108005678 ]

Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum

A. Pasquo, A. Bonamore, S. Franceschini, A. Macone, A. Boffi and A. Ilari

Synopsis: The cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from T. flavum, a protein which catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, are reported.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 284-288  [ doi:10.1107/S1744309108006374 ]

Crystallization and preliminary crystallographic analysis of the transpeptidase domain of penicillin-binding protein 2B from Streptococcus pneumoniae

M. Yamada, T. Watanabe, N. Baba, T. Miyara, J. Saito and Y. Takeuchi

Synopsis: The selenomethionyl-substituted transpeptidase domain of penicillin-binding protein (PBP) 2B from S. pneumoniae was isolated from a limited proteolysis digest of the soluble form of recombinant PBP 2B and then crystallized. MAD data were collected to 2.4 Å resolution.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 289-292  [ doi:10.1107/S1744309108006490 ]

Crystallization and preliminary X-ray diffraction analysis of the multidrug efflux transporter NorM from Neisseria gonorrhoeae

C.-C. Su, F. Long, G. McDermott, W. M. Shafer and E. W. Yu

Synopsis: The multidrug efflux transporter NorM from N. gonorrhoeae has been crystallized and X-ray diffraction data have been collected to a resolution of 6.5 Å.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 293-296  [ doi:10.1107/S1744309108006921 ]

Crystallization and preliminary X-ray diffraction studies of Seneca Valley Virus-001, a new member of the Picornaviridae family

S. Venkataraman, S. P. Reddy, J. Loo, N. Idamakanti, P. L. Hallenbeck and V. S. Reddy

Synopsis: Seneca Valley Virus-001 of the Picornavirdae family was crystallized in the space group R3 and X-ray diffraction data was collected to a resolution of 2.3 Å. Rotation-function studies suggested the presence of two distict sets of 20 protomers that belong to two different virus particles in the crystallographic asymmetric unit.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 297-299  [ doi:10.1107/S174430910800691X ]

Crystallization of an Lhx3-Isl1 complex

M. Bhati, M. Lee, A. L. Nancarrow, I. Bach, J. M. Guss and J. M. Matthews

Synopsis: An intramolecular complex comprising the LIM domains of Lhx3 and the interacting domain of Isl1 tethered by a flexible linker was engineered, overexpressed in E. coli, purified and crystallized.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 300-303  [ doi:10.1107/S1744309108007033 ]

Preliminary structural studies on the MtxX protein from Methanococcus jannaschii

D. H. Shin

Synopsis: In this study, the MtxX protein from M. jannaschii has been cloned, expressed, purified and crystallized.

Online 21 March 2008

 

Acta Cryst. (2008). F64, 304-306  [ doi:10.1107/S1744309108006775 ]

Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone

H. Morita, M. Tanio, S. Kondo, R. Kato, K. Wanibuchi, H. Noguchi, S. Sugio, I. Abe and T. Kohno

Synopsis: Benzalacetone synthase from R. palmatum has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 1.8 Å.

Online 29 March 2008

 

Acta Cryst. (2008). F64, 307-312  [ doi:10.1107/S1744309108006945 ]

Crystallization and preliminary X-ray analysis of the complex of the first von Willebrand type C domain bound to bone morphogenetic protein 2

L. Qiu, J. Zhang, A. Kotzsch, W. Sebald and T. D. Mueller

Synopsis: Crystals of the complex of the first von Willebrand type C domain (VWC1) of crossveinless 2 (CV2) bound to bone morphogenetic protein 2 (BMP2) exist in two tetragonal crystal forms belonging to either space group P4₁2₁2 or I4₁, with one complete BMP2 dimer and two CV2 VWC1 domains per asymmetric unit, and diffract to 2.6 Å resolution.

Online 29 March 2008

 

Acta Cryst. (2008). F64, 313-317  [ doi:10.1107/S1744309108007136 ]

Crystallization of hepatocyte nuclear factor 4 (HNF4) in complex with the HNF1 promoter element

P. Lu, J. Liu, M. Melikishvili, M. G. Fried and Y.-I. Chi

Synopsis: Sample preparation, characterization, crystallization and preliminary X-ray analysis are reported for the HNF4-DNA binary complex.

Online 29 March 2008

 

Acta Cryst. (2008). F64, 318-322  [ doi:10.1107/S1744309108007197 ]

Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis E virus-like particle

C.-Y. Wang, N. Miyazaki, T. Yamashita, A. Higashiura, A. Nakagawa, T.-C. Li, N. Takeda, L. Xing, E. Hjalmarsson, C. Friberg, D.-M. Liou, Y.-J. Sung, T. Tsukihara, Y. Matsuura, T. Miyamura and R. H. Cheng

Synopsis: A recombinant virus-like particle that is a potential oral hepatitis E vaccine was crystallized. Diffraction data were collected to 8.3 Å resolution and the X-ray structure was phased with the aid of a low-resolution density map determined using cryo-electron microscopy data.

Online 29 March 2008

 

Acta Cryst. (2008). F64, 323-325  [ doi:10.1107/S1744309108004612 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of thioredoxin Trx1 from Saccharomyces cerevisiae

Y. Zhang, R. Bao, C.-Z. Zhou and Y. Chen

Synopsis: In the present work, the cytosolic form of thioredoxin from S. cerevisiae was cloned, expressed, purified and crystallized.

Online 29 March 2008

 

Acta Cryst. (2008). F64, 326  [ doi:10.1107/S1744309108005666 ]

Protein Condensation

Online 29 March 2008