Structural Biology and Crystallization Communications

 

Acta Cryst. (2008). F64, 875-879  [ doi:10.1107/S1744309108027048 ]

Structure of a 6-pyruvoyltetrahydropterin synthase homolog from Streptomyces coelicolor

J. E. Spoonamore, S. A. Roberts, A. Heroux and V. Bandarian

Synopsis: The X-ray crystal structure of a 6-pyruvoyltetrahydropterin synthase homolog of unknown function has been determined at 1.5 Å resolution. The protein retains residues required for pterin binding, but nearly all catalytic residues are missing.

PDB reference: 3d7j

Online 30 September 2008

 

Acta Cryst. (2008). F64, 880-885  [ doi:10.1107/S1744309108028248 ]

There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization

D. Veesler, S. Blangy, C. Cambillau and G. Sciara

Synopsis: Crystals of an E. coli AcrB contaminant were grown from 95% pure CorA preparations. This very frequently occurring problem in membrane-protein crystallography laboratories is reported, as well as suggestions to avoid it.

PDB reference: 3d9b

Online 30 September 2008

 

Acta Cryst. (2008). F64, 886-892  [ doi:10.1107/S1744309108028546 ]

Structure and lability of archaeal dehydroquinase

N. N. Smith and D. T. Gallagher

Synopsis: The structure and thermal melting data for dehydroquinase from A. fulgidus are reported. The protein melts in vitro well below the organism's growth temperature.

PDB reference: 2ox1

Online 30 September 2008

 

Acta Cryst. (2008). F64, 893-898  [ doi:10.1107/S1744309108029102 ]

Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis

S. Lu, C. D. Smith, Z. Yang, P. S. Pruett, L. Nagy, D. McCombs, L. J. DeLucas, W. J. Brouillette and C. G. Brouillette

Synopsis: The crystal structure of apo nicotinic acid mononucleotide adenylyltransferase (NaMNAT) from B. anthracis was determined to 2.3 Å resolution and compared with other bacterial NaMNAT structures.

PDB reference: 3dv2

Online 30 September 2008

 

Acta Cryst. (2008). F64, 899-902  [ doi:10.1107/S1744309108026791 ]

Crystallization and preliminary X-ray analysis of the Thermoplasma acidophilum 20S proteasome in complex with protein substrates

K. Felderer, M. Groves, J. Diez, E. Pohl and S. Witt

Synopsis: Host-guest complexes of 20S proteasomes with either cytochrome c (cyt c) or green fluorescent protein (GFP) trapped inside the inner cavities of the proteasome have been crystallized. Diffraction data were collected to 3.4 Å (cyt c) and 3.8 Å (GFP) resolution using a synchrotron-radiation source.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 903-910  [ doi:10.1107/S1744309108026924 ]

Crystallization and preliminary crystallographic studies of putative threonyl-tRNA synthetases from Aeropyrum pernix and Sulfolobus tokodaii

S. Shimizu, E. C. M. Juan, Y. Miyashita, Y. Sato, M. M. Hoque, K. Suzuki, M. Yogiashi, M. Tsunoda, A.-C. Dock-Bregeon, D. Moras, T. Sekiguchi and A. Takénaka

Synopsis: Two types of putative threonyl-tRNA synthetases with complementary functions from the crenarchaea A. pernix K1 and S. tokodaii strain 7 have been overexpressed, purified and crystallized. The crystal structure of one of the four proteins, the catalytic type ThrRS from A. pernix, has successfully been solved by the Se-MAD method.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 911-913  [ doi:10.1107/S1744309108026869 ]

Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of membrane-type 1 matrix metalloproteinase (MT1-MMP)

S. Terawaki, K. Kitano, M. Aoyama and T. Hakoshima

Synopsis: The radixin FERM domain was shown to bind the MT1-MMP cytoplasmic peptide and crystals of the complex were obtained.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 914-917  [ doi:10.1107/S1744309108026870 ]

Purification, crystallization and preliminary crystallographic studies of SPCI-chymotrypsin complex at 2.8 Å resolution

A. J. da Silva, R. C. L. Teles, G. F. Esteves, C. R. dos Santos, J. A. R. G. Barbosa and S. M. de Freitas

Synopsis: S. parahyba chymotrypsin inhibitor in complex with chymotrypsin has been purified and crystallized using the vapour-diffusion method and preliminarily analysis has been performed using X-ray diffraction.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 918-921  [ doi:10.1107/S1744309108027036 ]

Isolation, crystallization and preliminary X-ray diffraction analysis of L-amino-acid oxidase from Vipera ammodytes ammodytes venom

D. Georgieva, A. Kardas, F. Buck, M. Perbandt and C. Betzel

Synopsis: A novel L-amino-acid oxidase was isolated from V. ammodytes ammodytes venom and crystallized. The solution conditions under which the protein sample was monodisperse were optimized using dynamic light scattering prior to crystallization. Preliminary diffraction data were collected to 2.6 Å resolution.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 922-925  [ doi:10.1107/S1744309108027462 ]

Expression, purification, crystallization and preliminary crystallographic study of the SRA domain of the human UHRF1 protein

B. Delagoutte, N. Lallous, C. Birck, P. Oudet and J.-P. Samama

Synopsis: The SET- and RING finger-associated (SRA) domain of human UHRF1 protein has been overexpressed in E. coli, purified and crystallized. The three-dimensional structure of the protein in its SeMet form was solved from 2.2 Å diffraction data using the multiple anomalous dispersion method.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 926-928  [ doi:10.1107/S1744309108027516 ]

Crystallization and preliminary X-ray diffraction analysis of the HsdR subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016

N. T. Uyen, K. Nishi, S.-Y. Park, J.-W. Choi, H.-J. Lee and J.-S. Kim

Synopsis: The crystallization of the HsdR subunit of a putative type I restriction-modification system from V. vulnificus and the collection of diffraction data to 2.6 Å resolution are reported.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 929-932  [ doi:10.1107/S1744309108027504 ]

Expression, purification, crystallization and preliminary X-ray diffraction studies of glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylococcus aureus (MRSA252)

S. Mukherjee, D. Dutta, B. Saha and A. K. Das

Synopsis: The cloning, overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) from MRSA252 are reported.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 933-935  [ doi:10.1107/S1744309108027796 ]

Expression, purification, crystallization and preliminary X-ray analysis of an archaeal protein homologous to plant nicotianamine synthase

C. Dreyfus, D. Pignol and P. Arnoux

Synopsis: A putative nicotianamine synthase from M. thermoautotrophicus was cloned, expressed, purified and crystallized. The native crystals diffracted to a resolution of 1.7 Å.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 936-941  [ doi:10.1107/S1744309108027930 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the CBS-domain pair from the Methanococcus jannaschii protein MJ0100

M. Lucas, D. Kortazar, E. Astigarraga, J. A. Fernández, J. M. Mato, M. L. Martínez-Chantar and L. A. Martínez-Cruz

Synopsis: The C-terminal domain of the Methanococcus jannaschii protein MJ0100 includes a CBS-domain pair and has been overexpressed, purified and crystallized. Crystals of selenomethionine-substituted (SeMet) protein were also grown.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 942-944  [ doi:10.1107/S1744309108028236 ]

Crystallization and preliminary X-ray diffraction studies of an RNA aptamer in complex with the human IgG Fc fragment

S. Sugiyama, Y. Nomura, T. Sakamoto, T. Kitatani, A. Kobayashi, S. Miyakawa, Y. Takahashi, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, Y. Nakamura and H. Matsumura

Synopsis: An RNA aptamer in complex with the human IgG Fc fragment have been crystallized. The stirring technique with a rotary shaker was used to improve the crystals and to ensure that they were of high quality and single, resulting in crystals that diffracted to 2.2 Å resolution.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 945-948  [ doi:10.1107/S1744309108028352 ]

Overexpression, purification, crystallization and preliminary X-ray crystal analysis of Bacillus pallidus D-arabinose isomerase

K. Takeda, H. Yoshida, G. Takada, K. Izumori and S. Kamitori

Synopsis: Recombinant B. pallidus D-arabinose isomerase was crystallized and diffraction data were collected to 2.3 Å resolution.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 949-953  [ doi:10.1107/S174430910802842X ]

Three crystal forms of the bifunctional enzyme proline utilization A (PutA) from Bradyrhizobium japonicum

J. P. Schuermann, T. A. White, D. Srivastava, D. B. Karr and J. J. Tanner

Synopsis: The first diffraction-quality crystals of a PutA protein are reported. One of the three crystal forms described here exhibits pseudo-merohedral twinning. Removal of the N-terminal histidine tag aided the crystallization of another form.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 954-956  [ doi:10.1107/S1744309108028522 ]

Initial crystallographic study of human PCNA in complex with a peptide containing the noncanonical PIP-box sequence of human DNA polymerase

A. Hishiki, T. Shimizu, T. Hanafusa, H. Ohmori, M. Sato and H. Hashimoto

Synopsis: Crystallization and diffraction studies of a human PCNA-Pol peptide complex are reported.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 957-959  [ doi:10.1107/S1744309108028571 ]

Crystallization and preliminary X-ray study of alkaline -mannanase from the alkaliphilic Bacillus sp. N16-5

Y. Zhao, Y. Zhang, F. Gao, Y. Xue, Y. Zeng and Y. Ma

Synopsis: The catalytic domain of an alkaline mannanase from the alkaliphilic Bacillus sp. N16-5 was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic analysis were performed for the recombinant enzyme.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 960-965  [ doi:10.1107/S1744309108028704 ]

Effect of leucine-to-methionine substitutions on the diffraction quality of histone chaperone SET/TAF-I/INHAT crystals

M. Senda, S. Muto, M. Horikoshi and T. Senda

Synopsis: The combination of leucine-to-methionine substitutions and optimization of cryoconditions improved the resolution of histone chaperone SET/TAF-I/INHAT crystals from around 5.5 to 2.3 Å without changing the crystallization conditions, allowing successful structure determination of SET/TAF-I/INHAT by the multiwavelength anomalous diffraction method.

Online 30 September 2008

 

Acta Cryst. (2008). F64, 966-969  [ doi:10.1107/S1744309108029515 ]

Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli

A. Baba-Dikwa, D. Thompson, N. J. Spencer, S. C. Andrews and K. A. Watson

Synopsis: The YncE protein of E. coli has been overproduced and purified and preliminary crystallographic analysis has been performed to 2.1 Å resolution. The structure reveals a seven-bladed -propeller fold.

Online 30 September 2008