Structural Biology and Crystallization Communications

 

Acta Cryst. (2008). F64, 970-973  [ doi:10.1107/S1744309108026754 ]

Structure of the ligand-binding domain of rat VDR in complex with the nonsecosteroidal vitamin D₃ analogue YR301

S. Kakuda, K. Okada, H. Eguchi, K. Takenouchi, W. Hakamata, M. Kurihara and M. Takimoto-Kamimura

Synopsis: The crystal structure of the ligand-binding domain of rat VDR in complex with a nonsecosteroidal vitamin D₃ analogue YR301 has been determined at 2.0 Å resolution.

PDB reference: 2zfx

Online 31 October 2008

 

Acta Cryst. (2008). F64, 974-977  [ doi:10.1107/S1744309108030297 ]

Crystallization and X-ray diffraction analysis of N-terminally truncated human ALG-2

H. Suzuki, M. Kawasaki, T. Kakiuchi, H. Shibata, S. Wakatsuki and M. Maki

Synopsis: Ca²⁺-bound and Zn²⁺-bound forms of ALG-2 were crystallized and X-ray diffraction data were collected to 3.1 and 3.3 Å resolution, respectively.

PDB references: 2zrs and 2zrt

Online 31 October 2008

 

Acta Cryst. (2008). F64, 978-985  [ doi:10.1107/S1744309108031679 ]

Rv0802c from Mycobacterium tuberculosis: the first structure of a succinyltransferase with the GNAT fold

M. W. Vetting, J. C. Errey and J. S. Blanchard

Synopsis: The structure of Rv0802c was determined in an unliganded form to 2.0 Å resolution utilizing single-wavelength anomalous dispersion from a samarium soak that resulted in a single bound Sm³⁺:citrate² complex.

PDB references: 2vzy and 2vzz

Online 31 October 2008

 

Acta Cryst. (2008). F64, 986-990  [ doi:10.1107/S1744309108033034 ]

Structure of Drosophila Mad MH2 domain

R. Hao, L. Chen, J.-W. Wu and Z.-X. Wang

Synopsis: The crystal structure of the MH2 domain of Mad, which closely matches those of other Smad MH2 domains, is reported at 3.2 Å resolution.

PDB reference: 3dit

Online 31 October 2008

 

Acta Cryst. (2008). F64, 991-996  [ doi:10.1107/S1744309108029667 ]

Phoenito experiments: combining the strengths of commercial crystallization automation

J. Newman, T. M. Pham and T. S. Peat

Synopsis: This paper describes how four general optimization techniques, growth-rate modulation, fine screening, seeding and additive screening, have been adapted for automation in a medium-throughput crystallization service facility.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 997-999  [ doi:10.1107/S1744309108028340 ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Streptococcus suis serotype 2

Q. Zhang, F. Gao, J. Qi, H. Cheng, Y. Liu and G. F. Gao

Synopsis: 2-Keto-3-deoxy-6-phosphogluconate aldolase from pathogenic S. suis, an attractive drug target, was cloned, overexpressed, purified and crystallized.

Online 25 October 2008

 

Acta Cryst. (2008). F64, 1000-1002  [ doi:10.1107/S1744309108030145 ]

Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides

I. Gómez García, C. L. Freel Meyers, C. T. Walsh and D. M. Lawson

Synopsis: Crystals of NovN, an O-carbamoyltransferase from S. spheroides, were obtained in monoclinic and orthorhombic forms and native X-ray data were recorded to a maximum of 2.3 Å resolution.

Online 25 October 2008

 

Acta Cryst. (2008). F64, 1003-1006  [ doi:10.1107/S1744309108029679 ]

Crystallization and preliminary neutron diffraction studies of HIV-1 protease cocrystallized with inhibitor KNI-272

H. Matsumura, M. Adachi, S. Sugiyama, S. Okada, M. Yamakami, T. Tamada, K. Hidaka, Y. Hayashi, T. Kimura, Y. Kiso, T. Kitatani, S. Maki, H. Y. Yoshikawa, H. Adachi, K. Takano, S. Murakami, T. Inoue, R. Kuroki and Y. Mori

Synopsis: In order to determine the protonation states of the residues within the active site of an HIV-1 protease-inhibitor complex, a crystal of HIV-1 protease complexed with inhibitor (KNI-272) was grown to a size of 1.4 mm³ for neutron diffraction study. The crystal diffracted to 2.3 Å resolution with sufficient quality for further structure determination.

Online 25 October 2008

 

Acta Cryst. (2008). F64, 1007-1009  [ doi:10.1107/S1744309108029692 ]

Crystallization and preliminary crystallographic analysis of exo--1,5-L-arabinofuranosidase from Streptomyces avermitilis NBRC14893

Z. Fujimoto, H. Ichinose and S. Kaneko

Synopsis: Exo--1,5-L-arabinofuranosidase from S. avermitilis NBRC14893 was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 2.2 Å and belonged to space group P2₁2₁2₁.

Online 25 October 2008

 

Acta Cryst. (2008). F64, 1010-1012  [ doi:10.1107/S1744309108029588 ]

Purification, crystallization and preliminary X-ray analysis of adenylylsulfate reductase from Desulfovibrio vulgaris Miyazaki F

H. Ogata, A. Goenka Agrawal, A. P. Kaur, R. Goddard, W. Gärtner and W. Lubitz

Synopsis: The crystallization of adenylylsulfate reductase isolated from the sulfate-reducing bacterium D. vulgaris Miyazaki F is described. The crystals diffracted to 1.7 Å resolution.

Online 25 October 2008

 

Acta Cryst. (2008). F64, 1013-1015  [ doi:10.1107/S1744309108028479 ]

Crystallization and preliminary characterization of dihydropteridine reductase from Dictyostelium discoideum

C. Chen, K. H. Seo, H. L. Kim, N. Zhuang, Y. S. Park and K. H. Lee

Synopsis: The dihydropteridine reductase from D. discoideum has been crystallized. Diffraction data were collected from a rectangular-shaped crystal to 2.16 Å resolution.

Online 25 October 2008

 

Acta Cryst. (2008). F64, 1016-1018  [ doi:10.1107/S1744309108025852 ]

Expression, crystallization and preliminary X-ray crystallographic analysis of Xoo2316, a predicted 6-phosphogluconolactonase, from Xanthomonas oryzae pv. oryzae

M.-K. Hong, J.-K. Kim, H. Kim, J. Jung, Y.-J. Ahn, J.-G. Kim, B.-M. Lee and L.-W. Kang

Synopsis: Xoo2316 from X. oryzae pv. oryzae, a predicted 6-phosphogluconolactonase, the second enzyme of the pentose phosphate pathway, has been cloned, purified and crystallized. A native data set was collected to 2.4 Å resolution and a MAD data set was collected to 2.5 Å resolution.

Online 28 October 2008

 

Acta Cryst. (2008). F64, 1019-1023  [ doi:10.1107/S1744309108031059 ]

Crystallization and preliminary X-ray analysis of the human respiratory syncytial virus nucleocapsid protein

K. El Omari, K. Scott, B. Dhaliwal, J. Ren, N. G. A. Abrescia, J. Budworth, M. Lockyer, K. L. Powell, A. R. Hawkins and D. K. Stammers

Synopsis: Crystals of the nucleocapsid protein of human respiratory syncytial virus have been obtained in two forms, the better of which diffracted to 3.6 Å resolution and contained ten subunits in the asymmetric unit.

Online 28 October 2008

 

Acta Cryst. (2008). F64, 1024-1026  [ doi:10.1107/S174430910803114X ]

Crystallization and preliminary X-ray analysis of isomaltase from Saccharomyces cerevisiae

K. Yamamoto, H. Miyake, M. Kusunoki and S. Osaki

Synopsis: The crystallization and preliminary X-ray analysis of isomaltase is reported.

Online 28 October 2008

 

Acta Cryst. (2008). F64, 1027-1030  [ doi:10.1107/S174430910803131X ]

Crystallization and preliminary diffraction studies of prostaglandin E₂-specific monoclonal antibody Fab fragment in the ligand complex

Y. Kurahashi, M. Sugahara, H. Ago, S. Aoyama, N. Takahashi, K. Takio, M. Katsukawa, S. Yamamoto and M. Miyano

Synopsis: The Fab fragment of a monoclonal anti-prostaglandin E₂ antibody was prepared and its complex with prostaglandin E₂ was crystallized.

Online 28 October 2008

 

Acta Cryst. (2008). F64, 1031-1033  [ doi:10.1107/S1744309108031631 ]

Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Xanthomonas oryzae pv. oryzae

P.-T.H. Ngo, J.-K. Kim, H. Kim, J. Jung, Y.-J. Ahn, J.-G. Kim, B.-M. Lee and L.-W. Kang

Synopsis: Peptide deformylase (PDF) catalyzes the removal of the N-formyl group from the N-terminus of nascent polypeptides. This process is essential for the survival of bacteria. As it is an antibacterial drug target, PDF from X. oryzae pv. oryzae was cloned, purified and crystallized for preliminary X-ray studies.

Online 28 October 2008

 

Acta Cryst. (2008). F64, 1034-1038  [ doi:10.1107/S1744309108031722 ]

Crystallization of Doc and the Phd-Doc toxin-antitoxin complex

A. Garcia-Pino, M.-H. Dao-Thi, E. Gazit, R. D. Magnuson, L. Wyns and R. Loris

Synopsis: Crystals of bacteriophage P1 Doc were grown in the free state, in complex with a 22-amino-acid C-terminal peptide of Phd and in complex with full-length Phd.

Online 28 October 2008

 

Acta Cryst. (2008). F64, 1039-1042  [ doi:10.1107/S1744309108031692 ]

Crystallographic characterization of the membrane-targeting domains of the Rac-specific guanine nucleotide-exchange factors Tiam1 and Tiam2

S. Terawaki, K. Kitano and T. Hakoshima

Synopsis: The membrane-targeting domains of the Rac-specific guanine nucleotide-exchange factors Tiam1 and Tiam2 were purified and crystallized.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1043-1045  [ doi:10.1107/S1744309108031928 ]

Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganism Bacillus thermocatenulatus

C. Carrasco-López, C. Godoy, B. de las Rivas, G. Fernández-Lorente, J. M. Palomo, J. M. Guisán, R. Fernández-Lafuente, M. Martínez-Ripoll and J. A. Hermoso

Synopsis: BTL2, a thermostable enantioselective biocatalyst of interest for industrial applications, has been crystallized using the sitting-drop vapour-diffusion method. Preliminary X-ray data to 2.2 Å resolution allowed the determination of its unit-cell parameters and space group and initiation of its structure determination.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1046-1048  [ doi:10.1107/S1744309108031898 ]

Crystallization of the coiled-coil domain of Atg16 essential for autophagy

Y. Fujioka, N. N. Noda, M. Matsushita, Y. Ohsumi and F. Inagaki

Synopsis: The coiled-coil domain of Atg16 was expressed, purified and crystallized. The resultant crystal diffracted to 2.5 Å resolution.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1049-1051  [ doi:10.1107/S1744309108031965 ]

Crystallization and preliminary X-ray diffraction analysis of PAT, an acetyltransferase from Sulfolobus solfataricus

C.-C. Cho, C.-W. Luo and C.-H. Hsu

Synopsis: PAT, an acetyltransferase from the archaeon S. solfataricus that specifically acetylates the chromatin protein Alba, was expressed, purified and crystallized.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1052-1055  [ doi:10.1107/S1744309108032132 ]

Purification, crystallization and data collection of Pectobacterium chrysanthemi AcsD, a type A siderophore synthetase

S. A. McMahon, M. Oke, H. Liu, K. A. Johnson, L. Carter, N. Kadi, M. F. White, G. L. Challis and J. H. Naismith

Synopsis: AcsD, an NRPS-independent siderophore synthetase, has been crystallized.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1056-1059  [ doi:10.1107/S1744309108032399 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the Whirly domain of StWhy2 in complex with single-stranded DNA

L. Cappadocia, J. Sygusch and N. Brisson

Synopsis: The Whirly domain of StWhy2 was crystallized both in the free form and in complex with single-stranded DNA.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1060-1062  [ doi:10.1107/S1744309108032454 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of Sagittaria sagittifolia arrowhead protease inhibitor API-A in complex with bovine trypsin

C. Jiang, R. Bao and Y. Chen

Synopsis: Arrowhead protease inhibitor A, a member of the serine protease inhibitor family, from S. sagittifolia has been cloned, expressed, purified and crystallized in complex with bovine trypsin.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1063-1065  [ doi:10.1107/S1744309108032569 ]

Expression, purification and crystallization of the ecto-enzymatic domain of rat E-NTPDase1 CD39

X. Zhong, M. Buddha, G. Guidotti, R. Kriz, W. Somers and L. Mosyak

Synopsis: The ecto-enzymatic domain of rat E-NTPDase1 CD39 was expressed and purified and diffraction-quality crystals of the enzyme were obtained.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1066-1069  [ doi:10.1107/S1744309108033046 ]

Purification, crystallization and X-ray diffraction analysis of pavine N-methyltransferase from Thalictrum flavum

A. Jain, J. Ziegler, D. K. Liscombe, P. J. Facchini, P. A. Tucker and S. Panjikar

Synopsis: The plant enzyme pavine N-methyltransferase from T. flavum has been produced in E. coli, purified and crystallized and its structure has been solved.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1070-1073  [ doi:10.1107/S174430910803306X ]

Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45

W. Zhang, M. Zhao, J. Li, X. Li, Z. Zeng and Z. Rao

Synopsis: The human Gadd45 protein has been crystallized as a prelude towards determination of its three-dimensional structure by X-ray crystallography.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1074-1078  [ doi:10.1107/S1744309108033290 ]

Crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 6

Q. Xie, H. M. Ongley, J. Hare and M. S. Chapman

Synopsis: Adeno-associated virus type 6, a human DNA virus that is being developed as a vector for gene therapy, has been crystallized in a form suitable for structure determination at about 3.2 Å resolution.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1079-1082  [ doi:10.1107/S1744309108033447 ]

Crystallization and preliminary X-ray analysis of the NKX2.5 homeodomain in complex with DNA

C. Genis, P. Scone, H. Kasahara and H.-J. Nam

Synopsis: The NKX2.5 homeodomain has been crystallized in complex with DNA. Diffraction data were collected to 1.7 Å resolution.

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1083-1086  [ doi:10.1107/S1744309108034039 ]

Expression, purification, crystallization and preliminary X-ray studies of the TAN1 orthologue from Methanothermobacter thermautotrophicus

A. P. G. Silva, R. T. Byrne, M. Chechik, C. Smits, D. G. Waterman and A. A. Antson

Synopsis: MTH909, the M. thermautotrophicus orthologue of S. cerevisiae TAN1, has been overexpressed, purified and crystallized. X-ray data were collected to 2.85 Å resolution from a crystal belonging to space group P6₁22 (or P6₅22).

Online 31 October 2008

 

Acta Cryst. (2008). F64, 1087-1090  [ doi:10.1107/S1744309108034180 ]

Purification, crystallization and preliminary crystallographic study of the putative enolase MJ0232 from the hyperthermophilic archaeon Methanococcus jannaschii

H. Yamamoto and N. Kunishima

Synopsis: The putative enolase MJ0232 from M. jannaschii was purified and crystallized. A native data set was collected to 1.85 Å resolution. Preliminary crystallographic analysis and a dynamic light-scattering experiment suggested biological relevance of the octameric state of MJ0232 in solution.

Online 31 October 2008