Structural Biology and Crystallization Communications

 

Acta Cryst. (2009). F65, 67-70  [ doi:10.1107/S1744309108037342 ]

Structure of the thioredoxin-fold domain of human phosducin-like protein 2

X. Lou, R. Bao, C.-Z. Zhou and Y. Chen

Synopsis: The X-ray crystal structure of the Trx-fold domain of hPDCL2 was solved at 2.70 Å resolution and resembled the Trx-fold domain of rat phosducin.

PDB reference: 3evi

Online 31 January 2009

 

Acta Cryst. (2009). F65, 71-74  [ doi:10.1107/S1744309108043078 ]

Structure of the ligand-binding domain of the EphB2 receptor at 2 Å resolution

Y. Goldgur, S. Paavilainen, D. Nikolov and J. P. Himanen

Synopsis: The crystal structure of the ligand-binding domain of a receptor tyrosine kinase EphB2, an important mediator of cell-cell communication, has been determined at a resolution of 2 Å. The structure confirms the induced-fit mechanism for the binding of ligands to EphB receptors.

PDB reference: 3etp

Online 31 January 2009

 

Acta Cryst. (2009). F65, 75-79  [ doi:10.1107/S1744309108043194 ]

Structure of human protein kinase CK22 with a potent indazole-derivative inhibitor

T. Nakaniwa, T. Kinoshita, Y. Sekiguchi, T. Tada, I. Nakanishi, K. Kitaura, Y. Suzuki, H. Ohno, A. Hirasawa and G. Tsujimoto

Synopsis: The crystal structure of human protein kinase CK22 with a potent indazole-derivative inhibitor has been determined at 3.2 Å resolution.

PDB reference: 3e3b

Online 31 January 2009

 

Acta Cryst. (2009). F65, 80-83  [ doi:10.1107/S1744309108044114 ]

Substrate binding induces structural changes in cytochrome P450cam

K. Sakurai, H. Shimada, T. Hayashi and T. Tsukihara

Synopsis: X-ray structures of ferric cytochrome P450cam partially complexed with the substrate (+)-camphor to two different extents were determined at 1.30-1.35 Å resolution, revealing the protein structures of the substrate-free and substrate-bound forms.

PDB references: 2zwt and 2zwu

Online 31 January 2009

 

Acta Cryst. (2009). F65, 84-92  [ doi:10.1107/S1744309109001110 ]

The first structure of a cold-adapted superoxide dismutase (SOD): biochemical and structural characterization of iron SOD from Aliivibrio salmonicida

H. L. Pedersen, N. P. Willassen and I. Leiros

Synopsis: The crystal structure of iron superoxide dismutase from the cold-adapted organism A. salmonicida has been determined at a resolution of 1.7 Å.

PDB reference: 2w7w

Online 31 January 2009

 

Acta Cryst. (2009). F65, 93-97  [ doi:10.1107/S1744309108042425 ]

Crystallization and X-ray analysis of human cytoplasmic phenylalanyl-tRNA synthetase

I. Finarov, N. Moor, N. Kessler and M. Safro

Synopsis: The crystallization and X-ray analysis of recombinant human cytosolic phenylalanyl-tRNA synthetase (hcPheRS) are reported. Diffraction data were collected to 3.3 Å resolution and the hcPheRS structure was determined by the molecular-replacement method using phase information derived from multiwavelength anomalous dispersion data.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 98-101  [ doi:10.1107/S1744309108042012 ]

Escherichia coli tRNA^Arg acceptor-stem isoacceptors: comparative crystallization and preliminary X-ray diffraction analysis

A. Eichert, A. Schreiber, J. P. Fürste, M. Perbandt, C. Betzel, V. A. Erdmann and C. Förster

Synopsis: Various E. coli tRNA^Arg acceptor-stem microhelix isoacceptors have been crystallized and investigated by high-resolution X-ray diffraction analysis.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 102-104  [ doi:10.1107/S1744309108042309 ]

Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP

L. Watanabe, P. R. de Moura, A. S. Nascimento, D. Colau, L. Dumoutier, J.-C. Renauld and I. Polikarpov

Synopsis: The human cytokine IL-22 has been crystallized in complex with its soluble decoy receptor IL-22BP. Diffraction data were collected to 2.75 Å resolution; the crystals belonged to the tetragonal space group P4₁.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 105-107  [ doi:10.1107/S1744309108041833 ]

Crystallization, data collection and data processing of maltose-binding protein (MalE) from the phytopathogen Xanthomonas axonopodis pv. citri

C. S. Souza, L. C. S. Ferreira, L. Thomas, J. A. R. G. Barbosa and A. Balan

Synopsis: The Xanthomonas axonopodis pv. citri maltose-binding protein MalE has been crystallized at 293 K using the hanging-drop vapour-diffusion method.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 108-110  [ doi:10.1107/S1744309108040682 ]

Crystallization and preliminary crystallographic analysis of the second RRM of Pub1 from Saccharomyces cerevisiae

Y. Cui, H. Li, X. Li, Y. Li, H. Zhou, L. Niu and M. Teng

Synopsis: The second RRM of Pub1 from S. cerevisiae was overexpressed, purified and crystallized. Diffraction data were collected to 1.69 Å resolution.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 111-115  [ doi:10.1107/S1744309108040578 ]

Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea

J. Kohoutová, I. Kutá Smatanová, J. Brynda, M. Lapkouski, J. L. Revuelta, J. B. Arellano and R. Ettrich

Synopsis: Degradation-free crystalization of thrombin-digested recombinant His-tagged PsbP protein of photosystem II from Spinacia oleracea resulting in crystals diffracting to 2.06 Å.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 116-119  [ doi:10.1107/S1744309108040219 ]

Crystallization and preliminary X-ray diffraction analysis of a complex between the electron-transfer partners hexameric Cu-containing nitrite reductase and pseudoazurin

D. Hira, M. Nojiri and S. Suzuki

Synopsis: The hexameric Cu-containing nitrite reductase and its electron-donor protein pseudoazurin have been cocrystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 3.3 Å resolution using a synchrotron-radiation source.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 120-122  [ doi:10.1107/S1744309108038505 ]

Purification, crystallization and preliminary X-ray diffraction studies on avian haemoglobin from pigeon (Columba livia)

P. Sathya Moorthy, K. Neelagandan, M. Balasubramanian and M. N. Ponnuswamy

Synopsis: Crystallization of pigeon haemoglobin at low pH (5.5) and high ionic concentration (1 M) using the hanging-drop vapour-diffusion method is reported.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 123-127  [ doi:10.1107/S1744309108040207 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the soluble domain of PPA0092, a putative nitrite reductase from Propionibacterium acnes

M. Nojiri, F. Shirota, D. Hira and S. Suzuki

Synopsis: The soluble domain of a putative copper-containing nitrite reductase from P. acnes has been overexpressed, purified and crystallized. The crystal belonged to space group P2₁3 and diffracted to 2.4 Å resolution.

Online 7 January 2009

 

Acta Cryst. (2009). F65, 128-132  [ doi:10.1107/S1744309108042127 ]

Preliminary structural investigations of the Eut-L shell protein of the ethanolamine ammonia-lyase metabolosome of Escherichia coli

K. Nikolakakis, A. Ohtaki, K. Newton, A. Chworos and M. Sagermann

Synopsis: Preliminary X-ray analysis of crystals of the bacterial microcompartment shell protein Eut-L from Escherichia coli is reported.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 133-135  [ doi:10.1107/S1744309108042474 ]

Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of a family 101 glycoside hydrolase from Streptococcus pneumoniae

K. J. Gregg and A. B. Boraston

Synopsis: The catalytic module of a family 101 glycoside hydrolase from S. pneumoniae was cloned, recombinantly produced and crystallized.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 136-139  [ doi:10.1107/S1744309108042516 ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the small subunit of isopropylmalate isomerase (Rv2987c) from Mycobacterium tuberculosis

M. Karuppasamy, A. Geerlof, L. Schuldt, C. Mueller-Dieckmann and M. S. Weiss

Synopsis: Two C-terminally truncated variants of the small subunit of isopropylmalate isomerase from M. tuberculosis have been cloned, expressed, purified, crystallized and examined by X-ray diffraction.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 140-143  [ doi:10.1107/S174430910804284X ]

Heterologous expression, crystallization and preliminary X-ray characterization of CcCel6C, a glycoside hydrolase family 6 enzyme from the basidiomycete Coprinopsis cinerea

Y. Kurakata, T. Tonozuka, Y. Liu, S. Kaneko, A. Nishikawa, K. Fukuda and M. Yoshida

Synopsis: Crystallization of CcCel6C was carried out by the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.6 Å resolution from the crystal.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 144-146  [ doi:10.1107/S1744309108042942 ]

Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2

T. Kuzuhara, D. Kise, H. Yoshida, T. Horita, Y. Murazaki, H. Utsunomiya and H. Tsuge

Synopsis: The C-terminal domain protein of the PB2 subunit of influenza A virus RNA-dependent RNA polymerase was expressed and crystallized and diffraction data were obtained from the crystals.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 147-150  [ doi:10.1107/S174430910804311X ]

Crystallization and preliminary X-ray analysis of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis

H. Tamura, H. Ashida, S. Koga, Y. Saito, T. Yadani, Y. Kai, T. Inoue, A. Yokota and H. Matsumura

Synopsis: Crystals of the 45.1 kDa functional form of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from B. subtilis diffracted to 2.30 Å resolution.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 151-153  [ doi:10.1107/S1744309108043431 ]

Crystallization and diffraction of an Lhx4-Isl2 complex

M. S. Gadd, D. B. Langley, J. M. Guss and J. M. Matthews

Synopsis: A complex comprising the LIM domains of the LIM-homeodomain protein Lhx4 tethered to a peptide region of Isl2 has been engineered, purified and crystallized. Crystals of this intramolecular complex diffracted to 2.16 Å resolution.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 154-158  [ doi:10.1107/S1744309108043534 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the transcriptional repressor SirR from Mycobacterium tuberculosis H37Rv

B. Saha, S. Mukherjee, D. Dutta and A. K. Das

Synopsis: The cloning, overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the transcriptional repressor SirR (staphylococcal iron regulator) from M. tuberculosis are reported.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 159-162  [ doi:10.1107/S1744309108044151 ]

Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixJ from Anabaena sp. PCC 7120

R. Narikawa, N. Muraki, T. Shiba, M. Ikeuchi and G. Kurisu

Synopsis: The chromophore-binding domain of cyanobacteriochrome AnPixJ was overproduced in E. coli, purified and crystallized in its red-absorbing form. Diffraction data were then collected to a resolution of 1.8 Å.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 163-165  [ doi:10.1107/S1744309108044187 ]

Expression, purification, crystallization and preliminary X-ray studies of the Ebola VP35 interferon inhibitory domain

D. W. Leung, N. D. Ginder, J. C. Nix, C. F. Basler, R. B. Honzatko and G. K. Amarasinghe

Synopsis: Native and selenomethionine-labeled crystals of Ebola VP35 interferon inhibitory domain were obtained by the hanging-drop vapor-diffusion method.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 166-168  [ doi:10.1107/S174430910900013X ]

Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4

J. Ju, J. Qi, S. Xu, K. Ohnishi, M. J. Benedik, Y. Xue and Y. Ma

Synopsis: An alkaline alanine racemase from alkaliphilic B. pseudofirmus OF4 was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic analysis were performed for the recombinant enzyme.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 169-172  [ doi:10.1107/S1744309109000153 ]

Crystallization and preliminary crystallographic analysis of N-acetyltransferase Mpr1 from Saccharomyces cerevisiae

T. Hibi, H. Yamamoto, G. Nakamura and H. Takagi

Synopsis: The yeast N-acetyltransferase that N-acetylates L-azetidine-2-carboxylic acid has been crystallized. Native data were collected to 1.9 Å resolution from a substrate-soaked crystal.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 173-176  [ doi:10.1107/S1744309109000360 ]

Preliminary X-ray crystallographic analysis of ornithine acetyltransferase (Rv1653) from Mycobacterium tuberculosis

R. Sankaranarayanan, C. R. Garen, M. M. Cherney, M. Yuan, C. Lee and M. N. G. James

Synopsis: Rv1653, an ornithine acetyltransferase from M. tuberculosis, has been crystallized and diffraction data have been collected to 1.7 Å resolution.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 177-183  [ doi:10.1107/S1744309109000372 ]

Twinned crystals of adeno-associated virus serotype 3b prove suitable for structural studies

T. F. Lerch, Q. Xie, H. M. Ongley, J. Hare and M. S. Chapman

Synopsis: Crystals of adeno-associated virus serotype 3b, a human DNA virus with promise as a vector for gene therapy, have been grown, diffract X-rays to 2.6 Å resolution and are suitable for structure determination in spite of twinning.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 184-187  [ doi:10.1107/S1744309109000566 ]

A preliminary neutron crystallographic study of proteinase K at pD 6.5

A. S. Gardberg, M. P. Blakeley and D. A. A. Myles

Synopsis: Preliminary neutron crystallographic data from the serine protease proteinase K have been recorded using the LADI-III diffractometer at the Institut Laue-Langevin. The results illustrate the feasibility of a full neutron structural analysis aimed at further understanding the catalytic mechanism of proteinase K.

Online 31 January 2009

 

Acta Cryst. (2009). F65, 188-191  [ doi:10.1107/S1744309109000670 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Bacillus anthracis in the presence of pyruvate

J. E. Voss, S. W. Scally, N. L. Taylor, C. Dogovski, M. R. Alderton, C. A. Hutton, J. A. Gerrard, M. W. Parker, R. C. J. Dobson and M. A. Perugini

Synopsis: Dihydrodipicolinate synthase (DHDPS) catalyses an important step in lysine biosynthesis. Here, the expression, purification, crystallization and preliminary diffraction analysis to 2.15 Å resolution of DHDPS from B. anthracis soaked with the substrate pyruvate are reported.

Online 31 January 2009