Structural Biology and Crystallization Communications

 

Acta Cryst. (2009). F65, 540-543  [ doi:10.1107/S1744309109014249 ]

Structure of AmpC -lactamase (AmpC^D) from an Escherichia coli clinical isolate with a tripeptide deletion (Gly286-Ser287-Asp288) in the H10 helix

Y. Yamaguchi, G. Sato, Y. Yamagata, Y. Doi, J. Wachino, Y. Arakawa, K. Matsuda and H. Kurosaki

Synopsis: The structure of AmpC -lactamase (AmpC^D) from E. coli with a tripeptide deletion (Gly286-Ser287-Asp288) is reported.

PDB reference: 2zj9

Online 22 May 2009

 

Acta Cryst. (2009). F65, 544-550  [ doi:10.1107/S1744309109017485 ]

ATP-dependent DNA ligase from Archaeoglobus fulgidus displays a tightly closed conformation

D. J. Kim, O. Kim, H.-W. Kim, H. S. Kim, S. J. Lee and S. W. Suh

Synopsis: The crystal structure of an ATP-dependent DNA ligase from A. fulgidus has been determined. It resembles, but shows some variations from, the closed conformation of DNA ligase from P. furiosus.

PDB reference: 3gde

Online 22 May 2009

 

Acta Cryst. (2009). F65, 551-558  [ doi:10.1107/S1744309109017722 ]

Comparison of GFL-GFR complexes: further evidence relating GFL bend angle to RET signalling

V. Parkash and A. Goldman

Synopsis: The second crystal structure of the GDNF-GFR1 complex provides further evidence that GFL signalling through RET is determined by the bend angle in the GFL.

PDB reference: 3fub

Online 23 May 2009

 

Acta Cryst. (2009). F65, 559-561  [ doi:10.1107/S1744309109014158 ]

Expression, crystallization and preliminary crystallographic analysis of the PAS domain of RsbP, a stress-response phosphatase from Bacillus subtilis

M. Makino, S. Kondo, T. Kaneko, S. Baba, K. Hirata and T. Kumasaka

Synopsis: The PAS domain of RsbP, a stress-response protein from B. subtilis, was crystallized using the sitting-drop vapour-diffusion method. The crystals belonged to space group P2₁ and diffraction data were collected to a resolution of 1.6 Å.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 562-564  [ doi:10.1107/S1744309109014687 ]

Purification, crystallization and preliminary X-ray analysis of L-sorbose reductase from Gluconobacter frateurii complexed with L-sorbose or NADPH

K. Kubota, K. Nagata, K. Miyazono, H. Toyama, K. Matsushita and M. Tanokura

Synopsis: NADPH-dependent L-sorbose reductase from G. frateurii (SR) was expressed, purified and crystallized with L-sorbose or NADPH using the sitting-drop vapour-diffusion method. Crystals of the SR-L-sorbose complex and SR-NADPH complex diffracted X-rays to 2.38 and 1.90 Å resolution, respectively.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 565-570  [ doi:10.1107/S1744309109014663 ]

Crystallization and preliminary crystallographic studies of putative RNA 3'-terminal phosphate cyclase from the crenarchaeon Sulfolobus tokodaii

S. Shimizu, M. Ohki, N. Okubo, K. Suzuki, M. Tsunoda, T. Sekiguchi and A. Takénaka

Synopsis: RNA 3'-terminal phosphate cyclase from the crenarchaeon S. tokodaii has been overexpressed and purified. The apo form, its selenomethionine derivative and their complexes with ATP under different conditions have been crystallized. The structure of the selenomethionine-labelled form has been successfully solved by the Se-MAD method.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 571-573  [ doi:10.1107/S1744309109015826 ]

Crystallization of Saccharomyces cerevisiae -mannosidase, a cargo protein of the Cvt pathway

Y. Watanabe, N. N. Noda, K. Honbou, K. Suzuki, Y. Sakai, Y. Ohsumi and F. Inagaki

Synopsis: Vacuolar -mannosidase, a cargo protein of the cytoplasm-to-vacuole targeting pathway, has been expressed, purified and crystallized.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 574-576  [ doi:10.1107/S1744309109014833 ]

Cloning, purification, crystallization and preliminary crystallographic analysis of a ribokinase from Staphylococcus aureus

L. Wang, H. Wang, J. Ruan, C. Tian, B. Sun and J. Zang

Synopsis: The ribokinase Sa239 from S. aureus has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.9 Å resolution.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 577-581  [ doi:10.1107/S1744309109015000 ]

Isolation, crystallization and preliminary X-ray analysis of the transamidosome, a ribonucleoprotein involved in asparagine formation

M. Bailly, M. Blaise, B. Lorber, S. Thirup and D. Kern

Synopsis: The isolation, crystallization and preliminary X-ray crystallographic data of the transamidosome from T. thermophilus, a ribonucleoprotein particle that provides the translational machinery with Asn-tRNA^Asn, are reported.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 582-585  [ doi:10.1107/S1744309109015759 ]

Purification, crystallization and preliminary X-ray analysis of the -lactamase Oih-1 from Oceanobacillus iheyensis

M. Toth, S. B. Vakulenko and C. A. Smith

Synopsis: Oih-1, a -lactamase enzyme isolated from the deep-sea bacterium O. iheyensis, has been crystallized and a complete X-ray diffraction data set has been collected to 1.65 Å resolution.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 586-588  [ doi:10.1107/S1744309109015802 ]

Crystallization and preliminary X-ray analysis of Escherichia coli RNase G

P. Fang, J. Wang, X. Li, M. Guo, L. Xing, X. Cao, Y. Zhu, Y. Gao, L. Niu and M. Teng

Synopsis: Full-length E. coli RNase G was overexpressed, purified and crystallized. Diffraction data were collected to a resolution of 3.4 Å.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 589-593  [ doi:10.1107/S1744309109015760 ]

Preliminary X-ray data analysis of crystalline hibiscus chlorotic ringspot virus

A. Cheng, J. A. Speir, Y. A. Yuan, J. E. Johnson and S.-M. Wong

Synopsis: Hibiscus chlorotic ringspot virus is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family. Authentic virus harvested from infected host kenaf leaves was purified and virus crystals were grown in multiple conditions. One of the crystals diffracted to 3.2 Å resolution and allowed the collection of a partial data set.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 594-596  [ doi:10.1107/S1744309109015899 ]

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of interleukin-4-inducing principle from Schistosoma mansoni eggs (IPSE/alpha-1)

H. Mayerhofer, G. Schramm, G. N. Hatzopoulos, C. Mueller-Dieckmann, H. Haas and J. Mueller-Dieckmann

Synopsis: Interleukin-4-inducing principle from S. mansoni eggs (IPSE/alpha-1) was cloned, purified and crystallized using the hanging-drop vapour-diffusion method at 293 K. X-ray diffraction data were collected to 1.66 Å resolution.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 597-600  [ doi:10.1107/S1744309109015735 ]

Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL

T. S. Walter, C. Liu, P. Huang, S. Zhang, L. R. Wedderburn, B. Gao, R. J. Owens, D. I. Stuart, P. Tang and J. Ren

Synopsis: Mouse RANKL and its receptor RANK have been cloned, expressed and purified. Crystals of RANK alone and in complex with RANKL have been obtained from which diffraction data have been collected to 2.0 and 2.8 Å resolution, respectively.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 601-603  [ doi:10.1107/S1744309109016133 ]

Crystallization and preliminary X-ray diffraction analysis of Salmonella typhimurium uridine phosphorylase complexed with 5-fluorouracil

A. A. Lashkov, A. G. Gabdoulkhakov, A. A. Shtil and A. M. Mikhailov

Synopsis: Uridine phosphorylase from S. typhimurium was expressed and purified and cocrystallized with the drug 5-fluorouracil. The crystals diffracted X-rays to 2.2 Å resolution using synchrotron radiation.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 604-607  [ doi:10.1107/S1744309109016200 ]

Crystallization and preliminary X-ray crystallographic analysis of two dimeric hyperthermostable thioredoxins isolated from Sulfolobus solfataricus

A. Ruggiero, M. A. Lanzotti, M. R. Ruocco, P. Grimaldi, D. Marasco, P. Arcari, M. Masullo, A. Zagari and L. Vitagliano

Synopsis: Two thioredoxins (SsTrxA2 and SsTrxA1) isolated from S. solfataricus have been crystallized. Diffraction data have been collected from SsTrxA2 and SsTrxA1 to 1.83 and 1.90 Å resolution, respectively. The structures of both enzymes have been solved by molecular replacement.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 608-611  [ doi:10.1107/S174430910901642X ]

Crystallographic characterization of the passenger domain of the Bordetella autotransporter BrkA

L. Zhao, N. T. Nguyen, R. C. Fernandez and M. E. P. Murphy

Synopsis: A secreted bacterial protein from a human pathogen that mediates serum resistance and adherence was overexpressed, purified, refolded and crystallized. Preliminary X-ray diffraction data are presented.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 612-614  [ doi:10.1107/S1744309109016649 ]

Crystallization and X-ray diffraction data collection of topoisomerase IV ParE subunit from Xanthomonas oryzae pv. oryzae

H. J. Shin, M. Yun, J.-Y. Song, H. J. Kim and Y.-S. Heo

Synopsis: The ParE subunit of topoisomerase IV from X. oryzae pv. oryzae was expressed, purified and crystallized. Diffraction data were collected to a resolution of 2.15 Å.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 615-617  [ doi:10.1107/S1744309109016571 ]

Crystallization and preliminary X-ray analysis of the V domain of human nectin-2

X. Qian, J. Qi, F. Chu, J. Liu, Q. Li and J. Yan

Synopsis: Crystals of the V domain of human nectin-2 diffracted to 1.85 Å resolution and were monoclinic, belonging to space group P2₁, with unit-cell parameters a = 52.3, b = 43.9, c = 56.1 Å, = 118.2°.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 618-620  [ doi:10.1107/S1744309109016704 ]

Crystallization of Mycobacterium smegmatis methionyl-tRNA synthetase in the presence of methionine and adenosine

H. Ingvarsson, T. A. Jones and T. Unge

Synopsis: The expression, purification and crystallization of methionyl-tRNA synthetase from Mycobacterium smegmatis. The crystals diffracted to 2.1 Å.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 621-624  [ doi:10.1107/S1744309109017023 ]

Crystallization and preliminary X-ray studies of the N-domain of the Wilson disease associated protein

L. Liu, C. O'Grady, S. A. Dalrymple, L. Prasad, O. Y. Dmitriev and L. T. J. Delbaere

Synopsis: The modified N-domain of the Wilson disease protein has been crystallized both in its native form and as a selenomethionine derivative. X-ray diffraction data were collected from the native crystal to 1.7 Å resolution, while the selenomethionine derivative diffracted to 2.7 Å resolution.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 625-628  [ doi:10.1107/S1744309109016820 ]

Crystallization of the flexible nuclear import receptor importin- in the unliganded state

N. Roman, B. Kirkby, M. Marfori, B. Kobe and J. K. Forwood

Synopsis: Conditions are reported for the crystallization of the flexible nuclear import receptor importin-. Preliminary X-ray diffraction data indicate that the structure can be solved using molecular replacement.

Online 22 May 2009

 

Acta Cryst. (2009). F65, 629-631  [ doi:10.1107/S1744309109017163 ]

Overexpression, purification, crystallization and preliminary X-ray studies of Vibrio cholerae EpsG

J. Jens, K. Raghunathan, F. Vago and D. Arvidson

Synopsis: Recombinant V. cholerae EpsG has been expressed, purified and crystallized. The crystals diffracted to 2.26 Å resolution.

Online 23 May 2009

 

Acta Cryst. (2009). F65, 632-634  [ doi:10.1107/S1744309109017230 ]

Crystallization and preliminary crystallographic analysis of -L-arabinopyranosidase from Streptomyces avermitilis NBRC14893

Z. Fujimoto, H. Ichinose, K. Harazono, M. Honda, A. Uzura and S. Kaneko

Synopsis: -L-Arabinopyranosidase from S. avermitilis NBRC14893 was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to 1.6 Å resolution and belonged to space group P2₁2₁2₁.

Online 23 May 2009

 

Acta Cryst. (2009). F65, 635-637  [ doi:10.1107/S174430910901745X ]

Isolation, purification, crystallization and preliminary crystallographic studies of amaryllin, a plant pathogenesis-related protein from Amaryllis belladonna

S. Kumar, N. Singh, M. Sinha, P. Kaur, A. Srinivasan, S. Sharma and T. P. Singh

Synopsis: A novel 15 kDa antifungal protein amaryllin has been crystallized using 30% PEG 8000 as the precipitating agent. The crystals belong to orthorhombic space group I or I2₁2₁2₁ with cell dimensions, a = 48.6, b = 61.9 and c = 79.6-Å.

Online 23 May 2009

 

Acta Cryst. (2009). F65, 638-640  [ doi:10.1107/S1744309109017643 ]

Crystallization of carbohydrate oxidase from Microdochium nivale

J. Dusková, J. Dohnálek, T. Skálová, L. H. Østergaard, C. C. Fuglsang, P. Kolenko, A. Stepánková and J. Hasek

Synopsis: Industrially used carbohydrate oxidase was successfully crystallized in several forms, diffraction data suitable for structural analysis were collected.

Online 23 May 2009

 

Acta Cryst. (2009). F65, 641-644  [ doi:10.1107/S1744309109017734 ]

Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain

C. R. dos Santos, M. R. Fessel, L. C. Vieira, M. A. Krieger, S. Goldenberg, B. G. Guimarães, N. I. T. Zanchin and J. A. R. G. Barbosa

Synopsis: The gene coding for Q4DV70 has been cloned and the protein overexpressed in Escherichia coli with an N-terminal His tag. Purification of Q4DV70 was carried out by affinity and size-exclusion chromatography and the His tag was removed by TEV protease digestion. Crystals of Q4DV70 were grown using the sitting-drop vapour-diffusion method.

Online 23 May 2009

 

Acta Cryst. (2009). F65, 645-647  [ doi:10.1107/S1744309109017588 ]

Crystallization and preliminary X-ray analysis of the NADPH-dependent 3-quinuclidinone reductase from Rhodotorula rubra

D. Takeshita, M. Kataoka, T. Miyakawa, K. Miyazono, A. Uzura, K. Nagata, S. Shimizu and M. Tanokura

Synopsis: The NADPH-dependent 3-quinuclidinone reductase from Rhodotorula rubra was expressed, purified, and crystallized and X-ray diffraction data of this crystal were collected to 2.2 Å resolution.

Online 23 May 2009

 

Acta Cryst. (2009). F65, 648-650  [ doi:10.1107/S1744309109018405 ]

Crystallization and preliminary crystallographic studies of human RIG-I in complex with double-stranded RNA

H. Moon and J. Choe

Synopsis: RIG-I is important in recognizing viral RNA and stimulating the immune system. Human RIG-I protein was crystallized in complex with 18-mer double-stranded RNA to study the recognition mechanism.

Online 23 May 2009

 

Acta Cryst. (2009). F65, 651-653  [ doi:10.1107/S1744309109018417 ]

Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5

Y. Wang, Y.-X. He, J. Yu and C.-Z. Zhou

Synopsis: Grx5 from Saccharomyces cerevisiae is a monothiol glutaredoxin that is involved in iron-sulfur cluster biogenesis. Here, yeast Grx5 was cloned and overproduced in Escherichia coli and the purified protein was crystallized using the hanging-drop vapour-diffusion method.

Online 23 May 2009