Structural Biology and Crystallization Communications

 

Acta Cryst. (2009). F65, 962  [ doi:10.1107/S174430910903855X ]

Laboratory Communications in Acta Crystallographica Section F

M. S. Weiss and H. Einspahr

Online 30 September 2009

 

Acta Cryst. (2009). F65, 963-966  [ doi:10.1107/S1744309109032813 ]

Structures of two truncated phage-tail hyaluronate lyases from Streptococcus pyogenes serotype M1

C. Martinez-Fleites, N. L. Smith, J. P. Turkenburg, G. W. Black and E. J. Taylor

Synopsis: The crystal structures of truncated forms of the Streptococcus pyogenes phage-encoded hyaluronate lyases HylP2 and HylP3 have been determined by molecular replacement to 1.6 and 1.9 Å resolution, respectively.

PDB references: 2wh7 and 2wb3

Online 18 September 2009

 

Acta Cryst. (2009). F65, 967-971  [ doi:10.1107/S1744309109032928 ]

Use of thallium to identify monovalent cation binding sites in GroEL

P. D. Kiser, G. H. Lorimer and K. Palczewski

Synopsis: Monovalent cation-binding sites in GroEL were identified utilizing the anomalous scattering signal from Tl⁺.

PDB reference: 3e76

Online 18 September 2009

 

Acta Cryst. (2009). F65, 972-977  [ doi:10.1107/S1744309109035027 ]

Structure of Mycobacterium tuberculosis Rv2714, a representative of a duplicated gene family in Actinobacteria

M. Graña, M. Bellinzoni, I. Miras, C. Fiez-Vandal, A. Haouz, W. Shepard, A. Buschiazzo and P. M. Alzari

Synopsis: The crystal structure of Rv2714, a protein of unknown function from M. tuberculosis, has been determined at 2.6 Å resolution using single-wavelength anomalous diffraction methods.

PDB reference: 2wam

Online 18 September 2009

 

Acta Cryst. (2009). F65, 978-986  [ doi:10.1107/S1744309109036033 ]

New structural insights and molecular-modelling studies of 4-methyl-5--hydroxyethylthiazole kinase from Pyrococcus horikoshii OT3 (PhThiK)

J. Jeyakanthan, S. Thamotharan, D. Velmurugan, V. S. N. Rao, S. Nagarajan, A. Shinkai, S. Kuramitsu and S. Yokoyama

Synopsis: The first crystal structure of 4-methyl-5--hydroxyethylthiazole kinase from an archaeon (P. horikoshii OT3) has been determined at 1.85 Å resolution. Comparative analyses of sequences and structures and modelling studies are presented.

PDB reference: 3hpd

Online 18 September 2009

 

Acta Cryst. (2009). F65, 987-991  [ doi:10.1107/S1744309109036616 ]

The structure of Staphylococcus aureus phosphopantetheine adenylyltransferase in complex with 3'-phosphoadenosine 5'-phosphosulfate reveals a new ligand-binding mode

H. H. Lee, H.-J. Yoon, J. Y. Kang, J. H. Park, D. J. Kim, K.-H. Choi, S.-K. Lee, J. Song, H.-J. Kim and S. W. Suh

Synopsis: The crystal structure of S. aureus phosphopantetheine adenylyltransferase in complex with 3'-phosphoadenosine 5'-phosphosulfate has been determined and reveals a new ligand-binding mode.

PDB reference: 3f3m

Online 23 September 2009

 

Acta Cryst. (2009). F65, 992-995  [ doi:10.1107/S1744309109036665 ]

High-resolution structure of human carbonic anhydrase II complexed with acetazolamide reveals insights into inhibitor drug design

K. H. Sippel, A. H. Robbins, J. Domsic, C. Genis, M. Agbandje-McKenna and R. McKenna

Synopsis: The crystal structure of human carbonic anhydrase II (CA II) complexed with acetazolamide (AZM) has been determined at 1.1 Å resolution. The co-binding of AZM and glycerol in the active site demonstrate that an isozyme specific CA inhibitor may be developed.

PDB reference: 3hs4

Online 25 September 2009

 

Acta Cryst. (2009). F65, 996-1000  [ doi:10.1107/S1744309109032333 ]

Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB

M. Tanabe and T. M. Iverson

Synopsis: The major outer membrane protein PorB from N. meningitidis was crystallized in three crystal forms; the best X-ray diffraction data were collected to 2.3 Å resolution.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1001-1003  [ doi:10.1107/S1744309109032321 ]

Purification and crystallization of a putative transcriptional regulator of the benzoate oxidation pathway in Burkholderia xenovorans LB400

A. M. Law, J. Bains and M. J. Boulanger

Synopsis: The X-ray diffraction and preliminary phasing of the putative transcriptional regulator Bxe_C0898 from B. xenovorans LB400 are reported.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1004-1006  [ doi:10.1107/S1744309109032370 ]

Expression, purification, crystallization and preliminary X-ray analysis of para-nitrophenol 4-monooxygenase from Pseudomonas putida DLL-E4

W. Liu, W. Shen, X. Zhao, H. Cao and Z. Cui

Synopsis: The crystallization and preliminary crystallographic analysis of a para-nitrophenol 4-monooxygenase PnpA from Pseudomonas putida DLL-E4 are presented.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1007-1010  [ doi:10.1107/S1744309109032540 ]

Crystallization and heavy-atom derivatization of StHsp14.0, a small heat-shock protein from Sulfolobus tokodaii

T. Hayashi, T. Abe, K. Takeda, N. Akiyama, M. Yohda and K. Miki

Synopsis: A variant of StHsp14.0, a small heat-shock protein from S. tokodaii, has been crystallized. Heavy-atom derivative crystals could be prepared by the cocrystallization method.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1011-1013  [ doi:10.1107/S1744309109032631 ]

Crystallization and preliminary X-ray diffraction analysis of crotoxin B from Crotalus durissus collilineatus venom

G. H. M. Salvador, C. A. H. Fernandes, L. C. Corrêa, N. A. Santos-Filho, A. M. Soares and M. R. M. Fontes

Synopsis: Crotoxin B is a basic phospholipase A₂ found in the venom of C. durissus collilineatus and is one of the subunits which constitute the crotoxin. Here, we present the crystallization, X-ray diffraction data collection and molecular-replacement solution of the dimeric complex formed by two crotoxin B isoforms.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1014-1017  [ doi:10.1107/S1744309109032576 ]

Crystallization and preliminary X-ray analysis of mannosyl-3-phosphoglycerate synthase from Thermus thermophilus HB27

S. Gonçalves, N. Borges, H. Santos and P. M. Matias

Synopsis: Mannosyl-3-phosphoglycerate synthase (MpgS) is a key enzyme in the biosynthesis of MG. Here, the purification, crystallization and preliminary crystallographic characterization of apo MpgS from Thermus thermophilus HB27 are reported.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1018-1020  [ doi:10.1107/S1744309109033016 ]

Crystallization and preliminary X-ray analysis of ZHE1, a hatching enzyme from the zebrafish Danio rerio

A. Okada, K. Nagata, K. Sano, S. Yasumasu, K. Kubota, J. Ohtsuka, I. Iuchi and M. Tanokura

Synopsis: The hatching enzyme of zebrafish, ZHE1, was expressed, purified and crystallized using the hanging-drop vapour-diffusion method. The crystal belonged to space group P2₁2₁2₁ and diffracted X-rays to a resolution of 1.14 Å.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1021-1023  [ doi:10.1107/S1744309109033156 ]

Crystallization and preliminary X-ray diffraction characterization of RpfF, a key DSF synthase from Stenotrophomonas maltophilia

X.-Y. Lin, S.-J. Liao, K.-H. Chin, H.-L. Shih, G.-H. Shen, A.H.-J. Wang and S.-H. Chou

Synopsis: Crystals of recombinant RpfF from S. maltophilia are tetragonal, space group P4₁2₁2 or P4₃2₁2, with unit-cell parameters a = 148.51, c = 122.82 Å, and diffract to 2.25 Å resolution.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1024-1026  [ doi:10.1107/S1744309109033831 ]

Crystallization and preliminary X-ray analysis of a D-Ala:D-Ser ligase associated with VanG-type vancomycin resistance

P. Weber, D. Meziane-Cherif, A. Haouz, F. A. Saul and P. Courvalin

Synopsis: The VanG D-alanine:D-serine ligase was crystallized in complex with ADP and diffraction data were collected at 2.35 Å resolution.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1027-1029  [ doi:10.1107/S1744309109034071 ]

Purification, crystallization and preliminary X-ray diffraction studies of parakeet (Psittacula krameri) haemoglobin

S. M. Jaimohan, M. D. Naresh, V. Arumugam and A. B. Mandal

Synopsis: Parakeet (Psittacula krameri) haemoglobin has been purified and crystallized under low salt buffered conditions. Preliminary analysis of the crystal that belonged to monoclinic system (C2) is reported.

Online 23 September 2009

 

Acta Cryst. (2009). F65, 1030-1034  [ doi:10.1107/S1744309109034459 ]

Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase

K. Homma and H. Moriyama

Synopsis: Algal Chlorella virus IL-3A deoxyuridine triphosphatase and its Glu81Ser plus Thr84Arg-mutated derivative, Mu-22, were crystallized using the hanging-drop vapor-diffusion method. Glycyl-seryl-tagged dUTPases yielded cubic and hexagonal crystals for IL-3A and Mu-22, respectively.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1035-1038  [ doi:10.1107/S1744309109034526 ]

Expression, purification and preliminary crystallographic analysis of Pseudomonas aeruginosa RocR protein

M. Kotaka, S. Dutta, H. C. Lee, M. J. M. Lim, Y. Wong, F. Rao, E. P. Mitchell, Z.-X. Liang and J. Lescar

Synopsis: P. aeruginosa RocR, an EAL-domain protein, has been expressed in E. coli, purified and crystallized. An X-ray diffraction data set was collected to a resolution of 2.50 Å.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1039-1041  [ doi:10.1107/S1744309109035118 ]

Crystallization and preliminary X-ray crystallographic studies of O-methyltransferase from Anabaena PCC 7120

G. Li, Z. Tang, G. Meng, K. Dai, J. Zhao and X. Zheng

Synopsis: The O-methyltransferase (OMT) from the Anabaena PCC 7120 has been overexpressed in a soluble form in E. coli, purified and crystallized. The crystals belonged to space group C222₁ and diffracted to 2.4 Å resolution.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1042-1047  [ doi:10.1107/S1744309109035386 ]

Towards the structure of the C-terminal part of the S-layer protein SbsC

M. Kroutil, T. Pavkov, R. Birner-Gruenberger, M. Tesarz, U. B. Sleytr, E. M. Egelseer and W. Keller

Synopsis: Various N-terminally truncated forms of the S-layer protein SbsC were crystallized and the form rSbsC_(755-1099), which was obtained by unintentional in situ proteolysis, was used for preliminary structure determination.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1048-1052  [ doi:10.1107/S1744309109035878 ]

A novel noncovalent complex of chorismate mutase and DAHP synthase from Mycobacterium tuberculosis: protein purification, crystallization and X-ray diffraction analysis

M. Ökvist, S. Sasso, K. Roderer, P. Kast and U. Krengel

Synopsis: Two shikimate-pathway enzymes from M. tuberculosis, the intracellular chorismate mutase (MtCM) and DAHP synthase (MtDS), were produced recombinantly and purified. MtCM was crystallized alone and in complex with MtDS and analyzed by X-ray diffraction.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1053-1055  [ doi:10.1107/S1744309109036173 ]

Preliminary X-ray crystallographic analysis of a nitric oxide-inducible lactate dehydrogenase from Staphylococcus aureus

J.-B. Dong, X. Liu, L.-F. Li, S. Wu and X.-D. Su

Synopsis: A nitric oxide-inducible lactate dehydrogenase from S. aureus, Sa-LDH-1, has been cloned, expressed, purified and crystallized in space group C2, with unit-cell parameters a = 131.4, b = 74.4, c = 103.2 Å, = 133.4°.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1056-1059  [ doi:10.1107/S1744309109036239 ]

Crystallization and preliminary X-ray diffraction characterization of an essential protein from Xanthomonas campestris that contains a noncanonical PilZ signature motif yet is critical for pathogenicity

T.-N. Li, K.-H. Chin, H.-L. Shih, A.H.-J. Wang and S.-H. Chou

Synopsis: An essential protein from the plant pathogen X. campestris pv. campestris (Xcc) that contains a noncanonical PilZ signature motif yet is critical for Xcc pathogenicity has been overexpressed in E. coli, purified and crystallized. The crystals diffracted to a resolution of 2.1 Å.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1060-1064  [ doi:10.1107/S1744309109036513 ]

Crystallization and preliminary X-ray diffraction analysis of the middle domain of Paip1

A. S. Kanaan, F. Frank, C. Maedler-Kron, K. Verma, N. Sonenberg and B. Nagar

Synopsis: The crystallization of the putative MIF4G domain of Paip1 is described. The crystals belonged to the monoclinic space group P2₁ and diffracted X-rays to beyond 2.2 Å resolution.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1065-1067  [ doi:10.1107/S1744309109036562 ]

Cloning, purification and preliminary X-ray crystallographic analysis of a hypothetical protein, MJ0754, from Methanococcus jannaschii DSM 2661

E. H. Lee, K. H. Nam and K. Y. Hwang

Synopsis: In this study, two forms of MJ0754 from the archaeon M. jannaschii were overexpressed and crystallized. The crystal of MJ0754 belonged to the hexagonal space group P6₁ and diffracted to 3.1 Å resolution, while the crystal of MJ0754t belonged to the orthogonal space group C222₁ and diffracted to 1.3 Å resolution using synchrotron radiation.

Online 25 September 2009

 

Acta Cryst. (2009). F65, 1068-1070  [ doi:10.1107/S1744309109036884 ]

How to get the magic triangle and the MAD triangle into your protein crystal

T. Beck, C. E. da Cunha and G. M. Sheldrick

Synopsis: The handling of the phasing tools I3C and B3C is described, emphasizing practical aspects such as the preparation of solutions and incorporation of the compounds into protein crystals.

PDB reference: 3iti

Online 25 September 2009