Structural Biology and Crystallization Communications

 

Acta Cryst. (2010). F66, 112  [ doi:10.1107/S1744309110001326 ]

In defence of our science - validation now!

E. N. Baker, Z. Dauter, H. Einspahr and M. S. Weiss

Online 28 January 2010

 

Acta Cryst. (2010). F66, 113-120  [ doi:10.1107/S174430910905194X ]

Structure of bovine pancreatic ribonuclease complexed with uridine 5'-monophosphate at 1.60 Å resolution

S. B. Larson, J. S. Day, C. Nguyen, R. Cudney and A. McPherson

Synopsis: The crystal structure of bovine pancreatic ribonuclease A in complex with uridine 5'-monophosphate was determined at 1.60 Å resolution. The uridine lies in the pyrimidine-specific binding site of the enzyme, with the phosphate group extended into the solvent without interacting with the protein.

PDB reference: 3jw1

Online 26 January 2010

 

Acta Cryst. (2010). F66, 121-129  [ doi:10.1107/S1744309109051938 ]

Structure of Bacillus amyloliquefaciens -amylase at high resolution: implications for thermal stability

J. Alikhajeh, K. Khajeh, B. Ranjbar, H. Naderi-Manesh, Y.-H. Lin, E. Liu, H.-H. Guan, Y.-C. Hsieh, P. Chuankhayan, Y.-C. Huang, J. Jeyaraman, M.-Y. Liu and C.-J. Chen

Synopsis: The crystal structure of B. amyloliquefaciens -amylase (BAA) at 1.4 Å resolution revealed ambiguities in the thermal adaptation of homologous proteins in this family.

PDB reference: 3bh4

Online 26 January 2010

 

Acta Cryst. (2010). F66, 130-136  [ doi:10.1107/S1744309109052063 ]

Structure of uracil-DNA N-glycosylase (UNG) from Vibrio cholerae: mapping temperature adaptation through structural and mutational analysis

I. L. U. Raeder, E. Moe, N. P. Willassen, A. O. Smalås and I. Leiros

Synopsis: The crystal structure of uracil-DNA N-glycosylase from Vibrio cholerae has been determined at 1.5 Å, and used for mapping temperature adaptation.

PDB reference: 2jhq

Online 26 January 2010

 

Acta Cryst. (2010). F66, 137-142  [ doi:10.1107/S1744309109052130 ]

A new crystal form of human diamine oxidase

A. P. McGrath, K. M. Hilmer, C. A. Collyer, D. M. Dooley and J. M. Guss

Synopsis: The structure of human diamine oxidase has been refined to 2.1 Å resolution in space group C222₁.

PDB reference: 3k5t

Online 27 January 2010

 

Acta Cryst. (2010). F66, 143-151  [ doi:10.1107/S1744309109052543 ]

A conserved mechanism of autoinhibition for the AMPK kinase domain: ATP-binding site and catalytic loop refolding as a means of regulation

D. R. Littler, J. R. Walker, T. Davis, L. E. Wybenga-Groot, P. J. Finerty, E. Newman, F. Mackenzie and S. Dhe-Paganon

Synopsis: A 1.9 Å resolution crystal structure of the isolated kinase domain from the 2 subunit of human AMPK, the first from a multicellular organism, is presented.

PDB reference: 2h6d

Online 27 January 2010

 

Acta Cryst. (2010). F66, 152-155  [ doi:10.1107/S1744309109052865 ]

A micromolar O-sulfated thiohydroximate inhibitor bound to plant myrosinase

A. Besle, X. Brazzolotto, A. Tatibouët, D. Cerniauskaite, E. Gallienne, P. Rollin and W. P. Burmeister

Synopsis: The crystal structure of an O-sulfated thiohydroximate inhibitor bound to S. alba myrosinase is reported.

PDB reference: 2wxd

Online 27 January 2010

 

Acta Cryst. (2010). F66, 156-159  [ doi:10.1107/S1744309109035581 ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the collagen-binding region of RspB from Erysipelothrix rhusiopathiae

A. S. Devi, Y. Ogawa, Y. Shimoji and K. Ponnuraj

Synopsis: The expression, purification and crystallization of the collagen-binding region of the E. rhusiopathiae surface protein RspB is described. The crystals diffracted to 2.2 Å resolution using synchrotron radiation.

Online 27 January 2010

 

Acta Cryst. (2010). F66, 160-162  [ doi:10.1107/S1744309109042614 ]

A preliminary X-ray study of D,D-heptose-1,7-bisphosphate phosphatase from Burkholderia thailandensis E264

M.-S. Kim and D. H. Shin

Synopsis: In this study, D,D-heptose-1,7-bisphosphate phosphatase has been cloned, expressed, purified and crystallized.

Online 27 January 2010

 

Acta Cryst. (2010). F66, 163-166  [ doi:10.1107/S174430910904932X ]

Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum

Y. Higashi, T. J. Smith, J. M. Jez and T. M. Kutchan

Synopsis: Recombinant P. somniferum salutaridine reductase (SalR) was purified and crystallized with NADPH using the hanging-drop vapor-diffusion method. Crystals of the SalR-NADPH complex diffracted X-rays to a resolution of 1.9 Å.

Online 27 January 2010

 

Acta Cryst. (2010). F66, 167-171  [ doi:10.1107/S1744309109051550 ]

Purification and crystallization of Phd, the antitoxin of the phd/doc operon

A. Garcia-Pino, Y. Sterckx, G. Vandenbussche and R. Loris

Synopsis: The antitoxin Phd from the phd/doc operon of bacteriophage P1 was crystallized in two distinct crystal forms.

Online 27 January 2010

 

Acta Cryst. (2010). F66, 172-176  [ doi:10.1107/S1744309109051616 ]

Crystallization and preliminary X-ray analysis of Na-SAA-2 from the human hookworm parasite Necator americanus

O. A. Asojo, G. N. Goud, B. Zhan, K. Ordonez, M. Sedlacek, K. Homma, V. Deumic, R. Gupta, J. Brelsford, M. K. Price, M. N. Ngamelue and P. J. Hotez

Synopsis: The purification, crystallization and preliminary X-ray diffraction analysis of a surface-associated antigen from the major human hookworm N. americanus is presented.

Online 27 January 2010

 

Acta Cryst. (2010). F66, 177-179  [ doi:10.1107/S1744309109051951 ]

Purification, crystallization and preliminary crystallographic analysis of the minor pilin FctB from Streptococcus pyogenes

C. Linke, P. G. Young, H. J. Kang, T. Proft and E. N. Baker

Synopsis: The minor pilin FctB from S. pyogenes strain 90/306S was expressed in E. coli, purified and crystallized. The hexagonal FctB crystals diffracted to 2.9 Å resolution.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 180-183  [ doi:10.1107/S1744309109052026 ]

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of the putative SAICAR synthetase (PH0239) from Pyrococcus horikoshii OT3

K. Manjunath, J. Jeyakanthan, N. Nakagawa, A. Shinkai, M. Yoshimura, S. Kuramitsu, S. Yokoyama and K. Sekar

Synopsis: SAICAR synthetase from P. horikoshii OT3 has been cloned, expressed, purified and crystallized.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 184-186  [ doi:10.1107/S1744309109052403 ]

Expression, purification and X-ray analysis of 1,3-propanediol dehydrogenase (Aq_1145) from Aquifex aeolicus VF5

J. Jeyakanthan, S. Thamotharan, S. Panjikar, Y. Kitamura, N. Nakagawa, A. Shinkai, S. Kuramitsu and S. Yokoyama

Synopsis: 1,3-Propanediol dehydrogenase (Aq_1145) from A. aeolicus VF5 has been overexpressed, purified and crystallized. The crystals diffracted to 2.4 Å resolution.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 187-191  [ doi:10.1107/S1744309109052828 ]

Crystallization and preliminary X-ray diffraction analyses of the homodimeric glycine decarboxylase (P-protein) from the cyanobacterium Synechocystis sp. PCC 6803

D. Hasse, M. Hagemann, I. Andersson and H. Bauwe

Synopsis: Recombinant glycine decarboxylase from Synechocystis sp. PCC 6803 was expressed in E. coli and purified to homogeneity. Crystals were obtained that diffracted to 2.1 Å resolution using synchrotron radiation.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 192-194  [ doi:10.1107/S1744309109052956 ]

Expression, purification and preliminary crystallographic analysis of recombinant human DEAD-box polypeptide 5

Y.-W. Choi, S. Dutta, B. C. Fielding and Y.-J. Tan

Synopsis: The production and crystallization of the human DEAD box polypeptide 5 are reported. A 2.7 Å native diffraction data set has been obtained.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 195-197  [ doi:10.1107/S1744309109053184 ]

Preliminary crystallographic characterization of the Grb2 SH2 domain in complex with a FAK-derived phosphotyrosyl peptide

H.-H. Chen, C.-W. Chen, Y.-Y. Chang, T.-L. Shen and C.-H. Hsu

Synopsis: Crystals of the Grb2 SH2 domain in complex with a phosphotyrosyl peptide corresponding to residues 921-930 of focal adhesion kinase (FAK) have been obtained using the sitting-drop vapour-diffusion technique. Data have been collected to 2.49 Å resolution.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 198-200  [ doi:10.1107/S1744309109053147 ]

Crystallization and preliminary X-ray crystallographic analysis of a highly specific serpin from the beetle Tenebrio molitor

S. H. Park, S. Piao, H.-M. Kwon, E.-H. Kim, B. L. Lee and N.-C. Ha

Synopsis: In this study, the mature form of SPN48 was overexpressed in Escherichia coli and purified. The purified SPN48 protein was crystallized using 14% polyethylene glycol 8000 and 0.1 M 2-(N-morpholino)ethanesulfonic acid pH 6.0 as the precipitant. The crystals diffracted X-rays to 2.1 Å resolution and were suitable for structure determination.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 201-203  [ doi:10.1107/S1744309109053688 ]

Crystallization and preliminary crystallographic analysis of recombinant VSP1 from Arabidopsis thaliana

Z.-B. Shi, H.-H. Ge, P. Zhao and M. Zhang

Synopsis: VSP1 from Arabidopsis thaliana was expressed in E. coli, purified and crystallized. X-ray diffraction data were collected to 1.9 Å resolution.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 204-206  [ doi:10.1107/S1744309109053846 ]

Crystallization and preliminary X-ray analysis of Psu, an inhibitor of the bacterial transcription terminator Rho

S. Khamrui, A. Ranjan, B. Pani, R. Sen and U. Sen

Synopsis: Psu, a unique 21 kDa protein from bacteriophage P4, is a non-essential capsid-decoration protein that inhibits Rho-dependent termination specifically and efficiently both in vivo and in vitro. Psu has been crystallized using PEG as precipitant and the crystals diffracted to 2.3 Å resolution.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 207-210  [ doi:10.1107/S1744309109052749 ]

Crystallization and preliminary crystallographic analysis of eukaryotic transcription and mRNA export factor Iws1 from Encephalitozoon cuniculi

M. Koch, M.-L. Diebold, J. Cavarelli and C. Romier

Synopsis: E. cuniculi Iws1, which acts in eukaryotic transcription and mRNA processing and export, has been crystallized and data have been collected to 2.5 Å resolution.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 211-213  [ doi:10.1107/S1744309110001077 ]

Crystallization and preliminary X-ray crystallographic analysis of human FAF1 UBX domain

W. Kang, H. Y. Shin and J. K. Yang

Synopsis: The FAF1 UBX domain was crystallized. X-ray diffraction data were collected to 3.00 Å resolution and the crystals belonged to space group F4₁32.

Online 28 January 2010

 

Acta Cryst. (2010). F66, 214  [ doi:10.1107/S1744309110001089 ]

Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1. Addendum

C. S. Hee, S. Gao, M. M. Miller, R. M. Goto, A. Ziegler, O. Daumke and B. Uchanska-Ziegler

Synopsis: Addendum to Hee et al. [Acta Cryst. (2009), F65, 422-425].

Online 28 January 2010