Structure of the newly found green turtle egg-white ribonuclease

The structure of GTRNase has been solved at 1.60 Å resolution by the molecular-replacement technique.

The structures of mutant forms of Hfq from Pseudomonas aeruginosa reveal the importance of the conserved His57 for the protein hexamer organization

The results obtained demonstrate the great importance of solvent-inaccessible conserved hydrogen bonds between the Hfq monomers in the stabilization of the hexamer structure.

X-ray analysis of bilirubin oxidase from Myrothecium verrucaria at 2.3 Å resolution using a twinned crystal

The crystal structure of bilirubin oxidase (BOD) from M. verrucaria has been determined at 2.3 Å resolution using a merohedrally twinned crystal. BOD has four copper-coordination sites that are almost identical to those of other multicopper oxidases and is also very similar to them in overall structure.

The structure of the hypothetical protein smu.1377c from Streptococcus mutans suggests a role in tRNA modification

The crystal structure of smu.1377c, a hypothetical protein from S. mutans, shows a similar fold to Sua5_YciO_YrdC-family proteins and indicates its functional role in tRNA modification.

The role of a topologically conserved isoleucine in gluta­thione transferase structure, stability and function

The role of a topologically conserved isoleucine in the structure of glutathione transferase was investigated by replacing the Ile71 residue in human GSTA1-1 by alanine or valine.

Structure of Rv1848 (UreA), the Mycobacterium tuberculosis urease γ subunit

Crystal and solution structures of Rv1848 protein and their implications in the biological assembly of Mtb urease is presented.

Structure of autophagy-related protein Atg8 from the silkworm Bombyx mori

The crystal structure of autophagy-related protein Atg8 from the silkworm B. mori has two additional helices at the N-terminus before the expected ubiquitin fold.

Crystallization and preliminary X-ray crystallographic analysis of BxlA, an intracellular β-D-xylosidase from Streptomyces thermoviolaceus OPC-520

BxlA from Streptomyces thermoviolaceus OPC-520 (molecular weight 82 kDa) was crystallized by the hanging-drop vapour-diffusion method at 289 K.

Crystallization and preliminary crystallographic analysis of the Clostridium perfringens enterotoxin

The crystallization of the C. perfringens enterotoxin is reported.

Crystallization and preliminary X-ray analysis of human endonuclease 1 (APE1) in complex with an oligonucleotide containing a 5,6-di­hydrouracil (DHU) or an α-anomeric 2′-deoxyadenosine (αdA) modified base

2.2 and 2.7 Å resolution data sets were collected from crystals of the human apurinic/apyrimidinic endonuclease 1 (APE1) truncated of the first 61 N-terminal residues in the presence of a 15-mer DNA containing an oxidatively damaged base as the target base. For both complexes, APE1 crystallized with a 6-mer DNA, suggesting that nucleotide incision and 3′→5′ exonuclease reactions occurred prior to crystallization.

Crystallization and preliminary X-ray analysis of L-azetidine-2-carboxylate hydrolase from Pseudomonas sp. strain A2C

L-Azetidine-2-carboxylate hydrolase from Pseudomonas sp. strain A2C was crystallized and diffraction data were collected to a resolution of 1.38 Å.

Preliminary X-ray crystallographic analysis of the D-xylulose 5-phosphate phospho­ketolase from Lactococcus lactis

The expression, purification, preliminary crystallization and crystallographic analysis of phosphoketolase from L. lactis ssp. lactis (strain IL 1403) are reported.

Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase

Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176.

Crystallization and preliminary X-ray analysis of a phosphopentomutase from Bacillus cereus

Two crystal forms of an Mn²⁺-dependent phosphopentomutase were identified from chemically distinct conditions by sparse-matrix screening with and without the inclusion of 50 mM Mn²⁺. The crystals identified in the presence of Mn²⁺ were of dramatically better diffraction quality than those identified in the absence of added Mn²⁺.

Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of human coronavirus OC43 nucleocapsid protein

Crystals of the N-terminal domain of nucleocapsid protein from human coronavirus OC43 were obtained that diffracted X-rays to a resolution of at least 1.7 Å.

Crystallization and preliminary X-ray analysis of the chemokine-binding protein from orf virus (Poxviridae)

The chemokine-binding protein from orf virus was purified and crystallized. The morphology and diffraction behaviour of these crystals was significantly improved through the use of additives known as Silver Bullets.

Crystallization and preliminary crystallographic analysis of the Magnetospirillum magneticum AMB-1 and M. gryphiswaldense MSR-1 magnetosome-associated proteins MamA

Here, the crystallization and preliminary X-ray analysis of recombinant and purified Magnetospirillum magneticum and M. gryphiswaldense MamA are reported for the first time.

Crystallization and preliminary X-ray diffraction studies of the putative haloalkane dehalogenase DppA from Plesiocystis pacifica SIR-I

The crystallization and preliminary X-ray diffraction studies of DppA from P. pacifica SIR-I are reported.

Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol de­hydrogenase I86A mutant

The secondary alcohol dehydrogenase mutant I86A from Thermoanaerobacter ethanolicus (TeSADH) was crystallized in novel crystallization conditions. Diffraction data to 3.2 Å were collected at the Canadian Light Source.

Purification, crystallization and preliminary crystallographic studies of Lys48-linked polyubiquitin chains

Lys48-linked tetraubiquitin, hexaubiquitin and octaubiquitin were enzymatically synthesized, purified and crystallized. X-ray diffraction data sets for tetraubiquitin and hexaubiquitin were collected at 1.6 and 1.8 Å resolution, respectively.

Crystallization and preliminary crystallographic analysis of Gre2p, an NADP⁺-dependent alcohol de­hydrogenase from Saccharomyces cerevisiae

The alcohol dehydrogenase Gre2p from S. cerevisiae catalyses the stereospecific reduction of a variety of different keto compounds and can therefore be applied as a valuable biocatalyst. The crystallization of the complex of Gre2p with NADP⁺ and its preliminary X-ray analysis are described.

Crystallization and preliminary X-ray diffraction studies of hyperthermophilic archaeal Rieske-type ferredoxin (ARF) from Sulfolobus solfataricus P1

A hyperthermophilic archaeal Rieske-type [2Fe–2S] ferredoxin (ARF) from S. solfataricus P1 has been crystallized as a recombinant protein with a vector-derived long N-terminal extension region. The P4₃2₁2 crystals of recombinant ARF diffracted to 1.85 Å resolution using synchrotron radiation.

High-quality crystals of human haematopoietic prostaglandin D synthase with novel inhibitors

High-quality crystals of human haematopoietic prostaglandin D synthase in complex with novel inhibitors were obtained in microgravity.

Crystallization and preliminary X-ray analysis of cecropin B from Bombyx mori

Cecropin B derived from the hemolymph of Bombyx mori has been crystallized by the hanging-drop vapour-diffusion method. The crystal diffracted to 1.43 Å resolution using X-ray radiation.

Fab crystallization and preliminary X-ray analysis of NC-1, an anti-HIV-1 antibody that recognizes the six-helix bundle core of gp41

The Fab fragment of NC-1, a murine antibody that specifically recognizes the six-helix bundle core of HIV-1 gp41, has been crystallized in space group P3₂21. An X-ray diffraction data set was collected at 3.2 Å resolution and a clear molecular-replacement solution was obtained for solution of the structure.

Characterization, crystallization and preliminary X-ray analysis of the adhesive domain of SdrE from Staphylococcus aureus

The adhesive domain of SdrE from Staphylococcus aureus was recombinantly expressed in Escherichia coli and crystallized. X-ray diffraction data were collected to 1.8 Å resolution.

Protein preparation, crystallization and preliminary X-ray analysis of Trypanosoma cruzi nucleoside diphosphate kinase 1

T. cruzi TcNDPK1 was overexpressed in Escherichia coli as an N-­terminally poly-His-tagged fusion protein and crystallized.