Structural Biology and Crystallization Communications

 

Acta Cryst. (2010). F66, 866-870  [ doi:10.1107/S1744309110019767 ]

Refined structures of placental alkaline phosphatase show a consistent pattern of interactions at the peripheral site

B. Stec, A. Cheltsov and J. L. Millán

Synopsis: Re-refinement of three models of human placental alkaline phosphatase resulted in improved models for the protein and led to proper definition of the bound ligands. The PNP molecules found in the new models have consistent interactions at the original peripheral site and an additional peripheral site.

PDB references: 3mk0, 3mk1 and 3mk2

Online 27 July 2010

 

Acta Cryst. (2010). F66, 871-877  [ doi:10.1107/S1744309110019330 ]

Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae

E. M. Gazdag, I. C. Cirstea, R. Breitling, J. Lukes, W. Blankenfeldt and K. Alexandrov

Synopsis: The structures of two new crystal forms of human Cu/Zn superoxide dismutase produced in the eukaryotic expression host L. tarentolae are reported.

PDB references: 3kh3 and 3kh4

Online 27 July 2010

 

Acta Cryst. (2010). F66, 878-880  [ doi:10.1107/S1744309110022529 ]

Structure of a fibronectin type III-like module from Clostridium thermocellum

M. Alahuhta, Q. Xu, R. Brunecky, W. S. Adney, S.-Y. Ding, M. E. Himmel and V. V. Lunin

Synopsis: The 1.6 Å resolution structure of a fibronectin type III-like module from Clostridium thermocellum (PDB code 3mpc) with two molecules in the asymmetric unit is reported.

PDB reference: 3mpc

Online 27 July 2010

 

Acta Cryst. (2010). F66, 881-886  [ doi:10.1107/S1744309110022852 ]

Structural studies of the catalytic core of the primate foamy virus (PFV-1) integrase

S. Réty, L. Rezábková, B. Dubanchet, J. Silhán, P. Legrand and A. Lewit-Bentley

Synopsis: The crystal structure of the catalytic core of the primate foamy virus integrase was solved to 2 Å resolution and in several different crystal forms. The effect of divalent cations and of mutations on the structure is discussed.

PDB references: 2x78, 2x74, 2x6n and 2x6s

Online 27 July 2010

 

Acta Cryst. (2010). F66, 887-892  [ doi:10.1107/S1744309110023043 ]

Structure of uracil-DNA glycosylase from Mycobacterium tuberculosis: insights into interactions with ligands

P. S. Kaushal, R. K. Talawar, U. Varshney and M. Vijayan

Synopsis: The molecule of uracil-DNA glycosylase from M. tuberculosis exhibits domain motion on binding to DNA or a proteinaceous inhibitor. The highly conserved DNA-binding region interacts with a citrate ion in the structure.

PDB reference: 3a7n

Online 27 July 2010

 

Acta Cryst. (2010). F66, 893-898  [ doi:10.1107/S1744309110023079 ]

Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) from Thermus thermophilus HB8

M. Kanagawa, S. Baba, A. Ebihara, A. Shinkai, K. Hirotsu, R. Mega, K. Kim, S. Kuramitsu, G. Sampei and G. Kawai

Synopsis: Structures of hypoxanthine-guanine phosphoribosyltransferase from T. thermophilus HB8 in the unliganded form, in complex with IMP and in complex with GMP are reported at 2.1, 1.9 and 2.2 Å resolution, respectively.

PDB references: 3acb, 3acc and 3acd

Online 27 July 2010

 

Acta Cryst. (2010). F66, 899-901  [ doi:10.1107/S1744309110012157 ]

Expression, purification, crystallization and preliminary X-ray crystallographic data from TktA, a transketolase from the lactic acid bacterium Lactobacillus salivarius

M. Horsham, H. Saxby, J. Blake, N. W. Isaacs, T. J. Mitchell and A. Riboldi-Tunnicliffe

Synopsis: Purified transketolase from L. salivarius has been crystallized using the vapour-diffusion technique.

Online 27 July 2010

 

Acta Cryst. (2010). F66, 902-904  [ doi:10.1107/S1744309110016726 ]

Cloning, purification, crystallization and preliminary crystallographic analysis of the tandem tudor domain of Sgf29 from Saccharomyces cerevisiae

J. Li, X. Xue, J. Ruan, M. Wu, Z. Zhu and J. Zang

Synopsis: The tandem tudor domain of Sgf29 from S. cerevisiae has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 1.92 Å resolution.

Online 27 July 2010

 

Acta Cryst. (2010). F66, 905-908  [ doi:10.1107/S1744309110018750 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of Pseudomonas aeruginosa MnmE, a GTPase involved in tRNA modification

H. H. Lee and S. W. Suh

Synopsis: MnmE from P. aeruginosa was crystallized and X-ray diffraction data were collected to 2.69 Å resolution.

Online 27 July 2010

 

Acta Cryst. (2010). F66, 909-912  [ doi:10.1107/S1744309110020518 ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2

X. Cheng, G. Lu, J. Qi, H. Cheng, F. Gao, J. Wang and J. Yan

Synopsis: Crystals of SAICAR synthase from S. suis serotype 2 were obtained in the presence of 40 mM aspartic acid substrate; they belonged to space group P2 and diffracted to 2.8 Å resolution.

Online 27 July 2010

 

Acta Cryst. (2010). F66, 913-915  [ doi:10.1107/S1744309110020695 ]

Cloning, purification, crystallization and preliminary crystallographic analysis of LsrR from Escherichia coli

X. Liu, M. Wu, D. Sun and J. Zang

Synopsis: The E. coli transcription repressor LsrR has been overexpressed, purified and crystallized. Diffraction data were collected to about 3 Å resolution.

Online 27 July 2010

 

Acta Cryst. (2010). F66, 916-918  [ doi:10.1107/S1744309110021068 ]

Expression, crystallization and preliminary X-ray diffraction analysis of the CMM2 region of the Arabidopsis thaliana Morpheus' molecule 1 protein

T. J. Petty, T. Nishimura, S. Emamzadah, C. Gabus, J. Paszkowski, T. D. Halazonetis and S. Thore

Synopsis: In order to investigate its function in transcriptional gene silencing, the highly conserved motif 2 from A. thaliana Morpheus' molecule 1 protein was expressed, purified and crystallized. X-ray diffraction analysis is reported to a resolution of 3.2 Å.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 919-922  [ doi:10.1107/S1744309110021032 ]

Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.

M. R. Tandang-Silvas, L. Carrazco-Peña, A. P. Barba de la Rosa, J. A. Osuna-Castro, S. Utsumi, B. Mikami and N. Maruyama

Synopsis: High level expression of soluble amaranth 11S proglobulin in Escherichia coli and its purification are described. Crystallization of the recombinant protein and crystal data collection are also presented.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 923-925  [ doi:10.1107/S1744309110021573 ]

Crystallization and preliminary X-ray crystallographic studies of omega-transaminase from Vibrio fluvialis JS17

T. Jang, B. Kim, O. K. Park, J. Y. Bae, B.-G. Kim, H. Yun and H. H. Park

Synopsis: The omega-transaminase from V. fluvialis JS17 was crystallized. Crystals were found to belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 78.43, b = 95.95, c = 122.89 Å. The crystals were obtained at 293 K and diffracted to a resolution of 2.5 Å.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 926-928  [ doi:10.1107/S1744309110021779 ]

Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of SET/TAF-I N from Homo sapiens

Z. Xu, W. Yang, N. Shi, Y. Gao, M. Teng and L. Niu

Synopsis: The SET/TAF-I that lacked the first 22 residues of the N-terminus from Homo sapiens was recombinantly expressed in Escherichia coli and crystallized. X-ray diffraction data were collected to 2.7 Å resolution.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 929-931  [ doi:10.1107/S1744309110016891 ]

Crystallization and preliminary crystallographic studies of the C-terminal domain of outer membrane protein A from enterohaemorrhagic Escherichia coli

J. Gu, X. Ji, J. Qi, Y. Ma, X. Mao and Q. Zou

Synopsis: In this study, recombinant OmpAC from EHEC was purified and crystallized and a diffraction data set was collected to 2.7 Å resolution.

Online 29 July 2010

    

Acta Cryst. (2010). F66, 932-934  [ doi:10.1107/S1744309110022463 ]

Crystallization and preliminary crystallographic analysis of the ADP-ribosyltransferase HopU1

Y. Lin, P. Wang, H. Yang and Y. Xu

Synopsis: Crystallization of the ADP-ribosyltransferase HopU1 is reported.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 935-937  [ doi:10.1107/S1744309110022657 ]

Crystallization and preliminary X-ray crystallographic studies of human FAIM protein

G. Li, L. Qu, G. Meng, X. Bai, K. Dai and X. Zheng

Synopsis: Full-length human Fas apoptosis inhibitory molecule (FAIM) and two truncation constructs have successfully been cloned, expressed and purified in Escherichia coli. FAIM (1-90) was crystallized and diffracted to a resolution of 2.5 Å.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 938-940  [ doi:10.1107/S1744309110022748 ]

Expression, purification, crystallization and preliminary X-ray studies of Lactobacillus jensenii enolase

P. T. Harris, K. Raghunathan, R. R. Spurbeck, C. G. Arvidson and D. N. Arvidson

Synopsis: Recombinant L. jensenii enolase has been expressed, purified and crystallized. The crystals diffracted to 3.25 Å resolution.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 941-943  [ doi:10.1107/S1744309110023845 ]

Overexpression, crystallization and preliminary X-ray analysis of xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from Bifidobacterium breve

R. Suzuki, B.-J. Kim, T. Shibata, Y. Iwamoto, T. Katayama, H. Ashida, T. Wakagi, H. Shoun, S. Fushinobu and K. Yamamoto

Synopsis: Xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from B. breve was overexpressed and crystallized. The crystals belonged to the tetragonal space group I422 and diffracted to beyond 1.7 Å resolution.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 944-947  [ doi:10.1107/S1744309110022797 ]

Cloning, overexpression, purification, crystallization and preliminary X-ray analysis of CheY3, a response regulator that directly interacts with the flagellar `switch complex' in Vibrio cholerae

S. Khamrui, M. Biswas, U. Sen and J. Dasgupta

Synopsis: A chemotaxis response regulator CheY3 from V. cholerae has been cloned, overexpressed, purified and crystallized. The crystals of CheY3 diffracted to 1.86 Å resolution.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 948-950  [ doi:10.1107/S1744309110023572 ]

Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids

H. Morita, K. Wanibuchi, R. Kato, S. Sugio and I. Abe

Synopsis: Curcuminoid synthase from O. sativa has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 2.5 Å.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 951-953  [ doi:10.1107/S1744309110024140 ]

Crystallization and preliminary X-ray diffraction analysis of the putative aldose 1-epimerase YeaD from Escherichia coli

W. You, X. Qiu, Y. Zhang, J. Ma, Y. Gao, X. Zhang, L. Niu and M. Teng

Synopsis: The putative aldose 1-epimerase YeaD from Escherichia coli was crystallized and diffraction data were collected to a resolution of 1.9 Å.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 954-956  [ doi:10.1107/S1744309110023900 ]

Crystallization and preliminary X-ray analysis of tubulin-folding cofactor A from Arabidopsis thaliana

L. Lu, J. Nan, W. Mi, C.-H. Wei, L.-F. Li and Y. Li

Synopsis: Tubulin-folding cofactor A from A. thaliana has been crystallized and preliminarily analyzed using X-ray diffraction.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 957-960  [ doi:10.1107/S1744309110024061 ]

Crystallization and preliminary X-ray diffraction analysis of Pseudomonas aeruginosa phosphorylcholine phosphatase

L. H. Otero, P. R. Beassoni, C. E. Domenech, A. T. Lisa and A. Albert

Synopsis: Phosphorylcholine phosphatase from P. aeruginosa has been crystallized. Diffraction data have been collected to 2.7 Å resolution.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 961-963  [ doi:10.1107/S1744309110024632 ]

Crystallization and preliminary X-ray analysis of the complex of human -thrombin with a modified thrombin-binding aptamer

I. Russo Krauss, A. Merlino, A. Randazzo, L. Mazzarella and F. Sica

Synopsis: The complex between human -thrombin and a modified thrombin-binding aptamer has been crystallized. Diffraction data were collected to 2.15 Å resolution, the structure was solved by molecular replacement and refinement of the model is in progress.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 964-968  [ doi:10.1107/S1744309110024681 ]

Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556

S. Harth, A. Kotzsch, W. Sebald and T. D. Mueller

Synopsis: The crystallization of BMP receptor type IA bound to the neutralizing antibody Fab fragment AbD1556 obtained by phage-display selection is reported.

Online 29 July 2010

 

Acta Cryst. (2010). F66, 969-972  [ doi:10.1107/S1744309110023857 ]

Laser-improved protein crystallization screening

N. Yennawar, S. Denev, V. Gopalan and H. Yennawar

Synopsis: The effect of laser irradiation on freshly prepared protein drops was explored and found to be a viable tool to induce nucleation. In our experiment, the number of positive crystallization hits during the screening process increased. In some instances the speed of crystal growth, the size of the crystals and the resolution of X-ray diffraction showed a marked improvement after laser exposure. Under optimized laser settings, in no case was the laser detrimental to crystal growth.

Online 30 July 2010