Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 66, Part 10 (October 2010)


Joint Center for Structural Genomics (JCSG) special issue


[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: The JCSG high-throughput structural biology pipeline.

JCSG pipeline: technology and dissemination


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Acta Cryst. (2010). F66, 1137-1142  [ doi:10.1107/S1744309110038212 ]

The JCSG high-throughput structural biology pipeline

M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley, J. Wooley, K. Wüthrich and I. A. Wilson

Synopsis: The Joint Center for Structural Genomics high-throughput structural biology pipeline has delivered more than 1000 structures to the community over the past ten years and has made a significant contribution to the overall goal of the NIH Protein Structure Initiative (PSI) of expanding structural coverage of the protein universe.

Online 30 September 2010


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Acta Cryst. (2010). F66, 1143-1147  [ doi:10.1107/S1744309110035736 ]

TOPSAN: use of a collaborative environment for annotating, analyzing and disseminating data on JCSG and PSI structures

S. S. Krishna, D. Weekes, C. Bakolitsa, M.-A. Elsliger, I. A. Wilson, A. Godzik and J. Wooley

Synopsis: Specific use cases of TOPSAN, an innovative collaborative platform for creating, sharing and distributing annotations and insights about protein structures, such as those determined by high-throughput structural genomics in the Protein Structure Initiative (PSI), are described. TOPSAN is the main annotation platform for JCSG structures and serves as a conduit for initiating collaborations with the biological community, as illustrated in this special issue of Acta Crystallographica Section F. Developed at the JCSG with the goal of opening a dialogue on the novel protein structures with the broader biological community, TOPSAN is a unique tool for fostering distributed collaborations and provides an efficient pathway to peer-reviewed publications.

Online 30 September 2010


domains of unknown function


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Acta Cryst. (2010). F66, 1148-1152  [ doi:10.1107/S1744309110001685 ]

DUFs: families in search of function

A. Bateman, P. Coggill and R. D. Finn

Synopsis: Domains of unknown function (DUFs) are a large set of uncharacterized protein families that structural genomics is helping biologists to understand functionally.

Online 5 March 2010


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Acta Cryst. (2010). F66, 1153-1159  [ doi:10.1107/S1744309109037749 ]

Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism

H.-J. Chiu, C. Bakolitsa, A. Skerra, A. Lomize, D. Carlton, M. D. Miller, S. S. Krishna, P. Abdubek, T. Astakhova, H. L. Axelrod, T. Clayton, M. C. Deller, L. Duan, J. Feuerhelm, J. C. Grant, S. K. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, A. Kumar, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, L. Okach, J. Paulsen, R. Reyes, C. L. Rife, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: NE1406, the first structural representative of PF09410, reveals a lipocalin-like fold with features that suggest involvement in lipid metabolism. In addition, NE1406 provides potential structural templates for two other protein families (PF07143 and PF08622).

PDB reference: 2ich

Online 8 December 2009


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Acta Cryst. (2010). F66, 1160-1166  [ doi:10.1107/S1744309110002514 ]

The structure of SSO2064, the first representative of Pfam family PF01796, reveals a novel two-domain zinc-ribbon OB-fold architecture with a potential acyl-CoA-binding role

S. S. Krishna, L. Aravind, C. Bakolitsa, J. Caruthers, D. Carlton, M. D. Miller, P. Abdubek, T. Astakhova, H. L. Axelrod, H.-J. Chiu, T. Clayton, M. C. Deller, L. Duan, J. Feuerhelm, J. C. Grant, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, A. Kumar, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, L. Okach, R. Reyes, C. L. Rife, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of SSO2064, the first structural representative of Pfam family PF01796 (DUF35), reveals a two-domain architecture comprising an N-terminal zinc-ribbon domain and a C-terminal OB-fold domain. Analysis of the domain architecture, operon organization and bacterial orthologs combined with the structural features of SSO2064 suggests a role involving acyl-CoA binding for this family of proteins.

PDB reference: 3irb

Online 5 March 2010


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Acta Cryst. (2010). F66, 1167-1173  [ doi:10.1107/S1744309110007517 ]

Structure of the first representative of Pfam family PF04016 (DUF364) reveals enolase and Rossmann-like folds that combine to form a unique active site with a possible role in heavy-metal chelation

M. D. Miller, L. Aravind, C. Bakolitsa, C. L. Rife, D. Carlton, P. Abdubek, T. Astakhova, H. L. Axelrod, H.-J. Chiu, T. Clayton, M. C. Deller, L. Duan, J. Feuerhelm, J. C. Grant, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, L. Okach, R. Reyes, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of the first representative of DUF364 family reveals a combination of enolase N-terminal-like and C-terminal Rossmann-like folds. Analysis of the interdomain cleft combined with sequence and genome context conservation among homologs, suggests a unique catalytic site likely involved in the synthesis of a flavin or pterin derivative.

PDB reference: 3l5o

Online 6 July 2010


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Acta Cryst. (2010). F66, 1174-1181  [ doi:10.1107/S1744309109022672 ]

The structure of the first representative of Pfam family PF09836 reveals a two-domain organization and suggests involvement in transcriptional regulation

D. Das, N. V. Grishin, A. Kumar, D. Carlton, C. Bakolitsa, M. D. Miller, P. Abdubek, T. Astakhova, H. L. Axelrod, P. Burra, C. Chen, H.-J. Chiu, M. Chiu, T. Clayton, M. C. Deller, L. Duan, K. Ellrott, D. Ernst, C. L. Farr, J. Feuerhelm, A. Grzechnik, S. K. Grzechnik, J. C. Grant, G. W. Han, L. Jaroszewski, K. K. Jin, H. A. Johnson, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, S. Oommachen, J. Paulsen, C. Puckett, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of the NGO1945 gene product from N. gonorrhoeae (UniProt Q5F5IO) reveals that the N-terminal domain assigned as a domain of unknown function (DUF2063) is likely to bind DNA and that the protein may be involved in transcriptional regulation.

PDB reference: 3dee

Online 27 October 2009


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Acta Cryst. (2010). F66, 1182-1189  [ doi:10.1107/S1744309109050647 ]

Structures of the first representatives of Pfam family PF06684 (DUF1185) reveal a novel variant of the Bacillus chorismate mutase fold and suggest a role in amino-acid metabolism

C. Bakolitsa, A. Kumar, K. K. Jin, D. McMullan, S. S. Krishna, M. D. Miller, P. Abdubek, C. Acosta, T. Astakhova, H. L. Axelrod, P. Burra, D. Carlton, C. Chen, H.-J. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, Y. Elias, K. Ellrott, D. Ernst, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, S. K. Grzechnik, G. W. Han, L. Jaroszewski, H. A. Johnson, H. E. Klock, M. W. Knuth, P. Kozbial, D. Marciano, A. T. Morse, K. D. Murphy, E. Nigoghossian, A. Nopakun, L. Okach, J. Paulsen, C. Puckett, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, C. V. Trout, H. van den Bedem, D. Weekes, A. White, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: Structures of the first representatives of PF06684 (DUF1185) reveal a Bacillus chorismate mutase-like fold with a potential role in amino-acid synthesis.

PDB references: 3byq and 2qtp

Online 5 March 2010


new folds


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Acta Cryst. (2010). F66, 1190-1197  [ doi:10.1107/S1744309110007177 ]

Structural classification of proteins and structural genomics: new insights into protein folding and evolution

A. Andreeva and A. G. Murzin

Synopsis: This review article surveys the protein structures determined by Joint Center for Structural Genomics and published in this special issue of Acta Crystallographica Section F.

Online 6 July 2010


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Acta Cryst. (2010). F66, 1198-1204  [ doi:10.1107/S1744309109025196 ]

The structure of Jann_2411 (DUF1470) from Jannaschia sp. at 1.45 Å resolution reveals a new fold (the ABATE domain) and suggests its possible role as a transcription regulator

C. Bakolitsa, A. Bateman, K. K. Jin, D. McMullan, S. S. Krishna, M. D. Miller, P. Abdubek, C. Acosta, T. Astakhova, H. L. Axelrod, P. Burra, D. Carlton, H.-J. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, Y. Elias, J. Feuerhelm, J. C. Grant, A. Grzechnik, S. K. Grzechnik, G. W. Han, L. Jaroszewski, H. E. Klock, M. W. Knuth, P. Kozbial, A. Kumar, D. Marciano, A. T. Morse, K. D. Murphy, E. Nigoghossian, L. Okach, S. Oommachen, J. Paulsen, R. Reyes, C. L. Rife, N. Sefcovic, H. Tien, C. B. Trame, C. V. Trout, H. van den Bedem, D. Weekes, A. White, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. Lesley and I. A. Wilson

Synopsis: The crystal structure of the first representative of the Pfam PF07336 (DUF1470) family reveals a two-domain organization that contains a new fold, termed the ABATE domain, at the N-terminus and a treble-clef zinc finger that is likely to bind DNA at the C-terminus.

PDB reference: 3h0n

Online 27 October 2009


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Acta Cryst. (2010). F66, 1205-1210  [ doi:10.1107/S1744309109023689 ]

Structure of LP2179, the first representative of Pfam family PF08866, suggests a new fold with a role in amino-acid metabolism

C. Bakolitsa, A. Kumar, D. Carlton, M. D. Miller, S. S. Krishna, P. Abdubek, T. Astakhova, H. L. Axelrod, H.-J. Chiu, T. Clayton, M. C. Deller, L. Duan, M.-A. Elsliger, J. Feuerhelm, S. K. Grzechnik, J. C. Grant, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, L. Okach, S. Oommachen, J. Paulsen, R. Reyes, C. L. Rife, H. J. Tien, C. V. Trout, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The first structural representative of the PF08866 (DUF1831) protein family reveals a potential new [alpha]+[beta] fold and indicates a possible involvement in amino-acid metabolism.

PDB reference: 2iay

Online 27 October 2009


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Acta Cryst. (2010). F66, 1211-1217  [ doi:10.1107/S1744309109022684 ]

The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism

C. Bakolitsa, A. Kumar, D. McMullan, S. S. Krishna, M. D. Miller, D. Carlton, R. Najmanovich, P. Abdubek, T. Astakhova, H.-J. Chiu, T. Clayton, M. C. Deller, L. Duan, Y. Elias, J. Feuerhelm, J. C. Grant, S. K. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, D. Marciano, A. T. Morse, E. Nigoghossian, L. Okach, S. Oommachen, J. Paulsen, R. Reyes, C. L. Rife, C. V. Trout, H. van den Bedem, D. Weekes, A. White, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: PA1994, a Pfam PF06475 (DUF1089) family homolog from P. aeruginosa, reveals remote similarities to lipoprotein localization factors and a conserved putative glycolipid-binding site.

PDB reference: 2h1t

Online 27 October 2009


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Acta Cryst. (2010). F66, 1218-1225  [ doi:10.1107/S1744309109050416 ]

Structures of the first representatives of Pfam family PF06938 (DUF1285) reveal a new fold with repeated structural motifs and possible involvement in signal transduction

G. W. Han, C. Bakolitsa, M. D. Miller, A. Kumar, D. Carlton, R. J. Najmanovich, P. Abdubek, T. Astakhova, H. L. Axelrod, C. Chen, H.-J. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, D. Ernst, J. Feuerhelm, J. C. Grant, A. Grzechnik, L. Jaroszewski, K. K. Jin, H. A. Johnson, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, L. Okach, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structures of SPO0140 and Sbal_2486 revealed a two-domain structure that adopts a novel fold. Analysis of the interdomain cleft suggests a nucleotide-based ligand with a genome context indicating signaling as a possible role for this family.

PDB references: 2re3 and 2ra9

Online 5 March 2010


novel variants of known folds and function


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Acta Cryst. (2010). F66, 1226-1229  [ doi:10.1107/S1744309110013242 ]

New variants of known folds: do they bring new biology?

E. V. Koonin

Synopsis: New distinct versions of known protein folds provide a powerful means of protein-function prediction that complements sequence and genomic context analysis.

Online 6 July 2010


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Acta Cryst. (2010). F66, 1230-1236  [ doi:10.1107/S1744309109022192 ]

Structure of an essential bacterial protein YeaZ (TM0874) from Thermotoga maritima at 2.5 Å resolution

Q. Xu, D. McMullan, L. Jaroszewski, S. S. Krishna, M.-A. Elsliger, A. P. Yeh, P. Abdubek, T. Astakhova, H. L. Axelrod, D. Carlton, H.-J. Chiu, T. Clayton, L. Duan, J. Feuerhelm, J. C. Grant, G. W. Han, K. K. Jin, H. E. Klock, M. W. Knuth, M. D. Miller, A. T. Morse, E. Nigoghossian, L. Okach, S. Oommachen, J. Paulsen, R. Reyes, C. L. Rife, H. van den Bedem, K. O. Hodgson, J. Wooley, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of an essential bacterial protein, YeaZ, from T. maritima identifies an interface that potentially mediates protein-protein interaction.

PDB reference: 2a6a

Online 27 October 2009


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Acta Cryst. (2010). F66, 1237-1244  [ doi:10.1107/S1744309110025534 ]

Structure of a putative NTP pyrophosphohydrolase: YP_001813558.1 from Exiguobacterium sibiricum 255-15

G. W. Han, M.-A. Elsliger, T. O. Yeates, Q. Xu, A. G. Murzin, S. S. Krishna, L. Jaroszewski, P. Abdubek, T. Astakhova, H. L. Axelrod, D. Carlton, C. Chen, H.-J. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, D. Ernst, J. Feuerhelm, J. C. Grant, A. Grzechnik, K. K. Jin, H. A. Johnson, H. E. Klock, M. W. Knuth, P. Kozbial, A. Kumar, W. W. Lam, D. Marciano, D. McMullan, M. D. Miller, A. T. Morse, E. Nigoghossian, L. Okach, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, K. O. Hodgson, J. Wooley, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of a putative NTP pyrophosphohydrolase, YP_001813558.1 from E. sibiricum, reveals a novel segment-swapped linked-dimer assembly.

PDB reference: 3nl9

Online 4 August 2010


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Acta Cryst. (2010). F66, 1245-1253  [ doi:10.1107/S1744309109042481 ]

Open and closed conformations of two SpoIIAA-like proteins (YP_749275.1 and YP_001095227.1) provide insights into membrane association and ligand binding

A. Kumar, A. Lomize, K. K. Jin, D. Carlton, M. D. Miller, L. Jaroszewski, P. Abdubek, T. Astakhova, H. L. Axelrod, H.-J. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, L. Okach, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structures of two orthologous proteins from different Shewanella species have uncovered a resemblance to CRAL-TRIO carrier proteins, which suggest that they function as transporters of small nonpolar molecules. One protein adopts an open conformation, while the other adopts a closed structure that may act as a conformational switch in the transport of ligands at the membrane surface.

PDB references: 2ook and 2q3l

Online 8 December 2009


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Acta Cryst. (2010). F66, 1254-1260  [ doi:10.1107/S1744309109018168 ]

The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold

D. Das, P. Kozbial, G. W. Han, D. Carlton, L. Jaroszewski, P. Abdubek, T. Astakhova, H. L. Axelrod, C. Bakolitsa, C. Chen, H.-J. Chiu, M. Chiu, T. Clayton, M. C. Deller, L. Duan, K. Ellrott, M.-A. Elsliger, D. Ernst, C. L. Farr, J. Feuerhelm, A. Grzechnik, J. C. Grant, K. K. Jin, H. A. Johnson, H. E. Klock, M. W. Knuth, S. S. Krishna, A. Kumar, D. Marciano, D. McMullan, M. D. Miller, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, S. Oommachen, J. Paulsen, C. Puckett, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, Q. Xu, K. O. Hodgson, J. Wooley, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: KPN03535 is a protein unique to K. pneumoniae. The crystal structure reveals that KPN03535 represents a novel variant of the OB-fold and is likely to be a DNA-binding lipoprotein.

PDB reference: 3f1z

Online 27 October 2009


human gut microbiome


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Acta Cryst. (2010). F66, 1261-1264  [ doi:10.1107/S1744309110029088 ]

Viewing the human microbiome through three-dimensional glasses: integrating structural and functional studies to better define the properties of myriad carbohydrate-active enzymes

P. J. Turnbaugh, B. Henrissat and J. I. Gordon

Synopsis: Metagenomics has unleashed a deluge of sequencing data describing the organismal, genetic, and transcriptional diversity of the human microbiome. To better understand the precise functions of the myriad proteins encoded by the microbiome, including carbohydrate-active enzymes, it will be critical to combine structural studies with functional analyses.

Online 31 July 2010


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Acta Cryst. (2010). F66, 1265-1273  [ doi:10.1107/S1744309109046788 ]

The structure of BVU2987 from Bacteroides vulgatus reveals a superfamily of bacterial periplasmic proteins with possible inhibitory function

D. Das, R. D. Finn, D. Carlton, M. D. Miller, P. Abdubek, T. Astakhova, H. L. Axelrod, C. Bakolitsa, C. Chen, H.-J. Chiu, M. Chiu, T. Clayton, M. C. Deller, L. Duan, K. Ellrott, D. Ernst, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, C. Puckett, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of the BVU2987 gene product from B. vulgatus (UniProt A6L4L1) reveals that members of the new Pfam family PF11396 (domain of unknown function; DUF2874) are similar to [beta]-lactamase inhibitor protein and YpmB.

PDB reference: 3due

Online 5 March 2010


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Acta Cryst. (2010). F66, 1274-1280  [ doi:10.1107/S1744309110032999 ]

Structure of BT_3984, a member of the SusD/RagB family of nutrient-binding molecules

C. Bakolitsa, Q. Xu, C. L. Rife, P. Abdubek, T. Astakhova, H. L. Axelrod, D. Carlton, C. Chen, H.-J. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, K. Ellrott, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, W. W. Lam, D. Marciano, D. McMullan, M. D. Miller, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, C. Puckett, R. Reyes, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of BT_3984, a SusD-family protein, reveals a TPR N-terminal region providing support for a loop-rich C-terminal subdomain and suggests possible interfaces involved in sus complex formation.

PDB reference: 3cgh

Online 22 September 2010


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Acta Cryst. (2010). F66, 1281-1286  [ doi:10.1107/S1744309110006548 ]

A conserved fold for fimbrial components revealed by the crystal structure of a putative fimbrial assembly protein (BT1062) from Bacteroides thetaiotaomicron at 2.2 Å resolution

Q. Xu, P. Abdubek, T. Astakhova, H. L. Axelrod, C. Bakolitsa, X. Cai, D. Carlton, C. Chen, H.-J. Chiu, M. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, K. Ellrott, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, D. Marciano, D. McMullan, M. D. Miller, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, C. Puckett, R. Reyes, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, A. Yeh, J. Zhou, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of BT1062 from Bacteroides thetaiotaomicron revealed a conserved fold that is widely adopted by fimbrial components.

PDB reference: 3gf8

Online 6 July 2010


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Acta Cryst. (2010). F66, 1287-1296  [ doi:10.1107/S1744309110028228 ]

Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism

A. P. Yeh, P. Abdubek, T. Astakhova, H. L. Axelrod, C. Bakolitsa, X. Cai, D. Carlton, C. Chen, H.-J. Chiu, M. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, K. Ellrott, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, W. W. Lam, D. Marciano, D. McMullan, M. D. Miller, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, C. Puckett, R. Reyes, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of BT2081 from B. thetaiotaomicron reveals a two-domain protein with a putative carbohydrate-binding site in the C-terminal domain.

PDB reference: 3hbz

Online 4 August 2010


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Acta Cryst. (2010). F66, 1297-1305  [ doi:10.1107/S1744309110023055 ]

Structure of a membrane-attack complex/perforin (MACPF) family protein from the human gut symbiont Bacteroides thetaiotaomicron

Q. Xu, P. Abdubek, T. Astakhova, H. L. Axelrod, C. Bakolitsa, X. Cai, D. Carlton, C. Chen, H.-J. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, K. Ellrott, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, W. W. Lam, D. Marciano, M. D. Miller, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, C. Puckett, R. Reyes, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, A. Yeh, J. Zhou, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of a novel MACPF protein, which may play a role in the adaptation of commensal bacteria to host environments in the human gut, was determined and analyzed.

PDB reference: 3kk7

Online 31 July 2010


ligands that aid in function characterization


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Acta Cryst. (2010). F66, 1306-1308  [ doi:10.1107/S1744309110035748 ]

Ligands in crystal structures that aid in functional characterization

A. E. Speers and B. F. Cravatt

Synopsis: An overview and commentary on the value of liganded structures emerging from the JCSG structural genomics initiative.

Online 30 September 2010


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Acta Cryst. (2010). F66, 1309-1316  [ doi:10.1107/S1744309110008092 ]

Ligands in PSI structures

A. Kumar, H.-J. Chiu, H. L. Axelrod, A. Morse, M.-A. Elsliger, I. A. Wilson and A. Deacon

Synopsis: A survey of the types and frequency of ligands that are bound to PSI structures is analyzed as well as their utility in functional annotation of previously uncharacterized proteins.

Online 6 July 2010


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Acta Cryst. (2010). F66, 1317-1325  [ doi:10.1107/S1744309110021688 ]

The structure of Haemophilus influenzae prephenate dehydrogenase suggests unique features of bifunctional TyrA enzymes

H.-J. Chiu, P. Abdubek, T. Astakhova, H. L. Axelrod, D. Carlton, T. Clayton, D. Das, M. C. Deller, L. Duan, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, D. Marciano, D. McMullan, M. D. Miller, A. T. Morse, E. Nigoghossian, L. Okach, R. Reyes, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of the prephenate dehydrogenase component of the bifunctional H. influenzae TyrA reveals unique structural differences between bifunctional and monofunctional TyrA enzymes.

PDB reference: 2pv7

Online 31 July 2010


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Acta Cryst. (2010). F66, 1326-1334  [ doi:10.1107/S1744309110037619 ]

Structure of a tryptophanyl-tRNA synthetase containing an iron-sulfur cluster

G. W. Han, X.-L. Yang, D. McMullan, Y. E. Chong, S. S. Krishna, C. L. Rife, D. Weekes, S. M. Brittain, P. Abdubek, E. Ambing, T. Astakhova, H. L. Axelrod, D. Carlton, J. Caruthers, H.-J. Chiu, T. Clayton, L. Duan, J. Feuerhelm, J. C. Grant, S. K. Grzechnik, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, A. Kumar, D. Marciano, M. D. Miller, A. T. Morse, E. Nigoghossian, L. Okach, J. Paulsen, R. Reyes, H. van den Bedem, A. White, G. Wolf, Q. Xu, K. O. Hodgson, J. Wooley, A. M. Deacon, A. Godzik, S. A. Lesley, M.-A. Elsliger, P. Schimmel and I. A. Wilson

Synopsis: The crystal structure of tryptophanyl-tRNA synthetase from T. maritima unexpectedly revealed an iron-sulfur cluster bound to the tRNA anticodon-binding region.

PDB reference: 2g36

Online 23 September 2010


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Acta Cryst. (2010). F66, 1335-1346  [ doi:10.1107/S1744309110020166 ]

Structures of three members of Pfam PF02663 (FmdE) implicated in microbial methanogenesis reveal a conserved [alpha]+[beta] core domain and an auxiliary C-terminal treble-clef zinc finger

H. L. Axelrod, D. Das, P. Abdubek, T. Astakhova, C. Bakolitsa, D. Carlton, C. Chen, H.-J. Chiu, T. Clayton, M. C. Deller, L. Duan, K. Ellrott, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, W. W. Lam, D. Marciano, D. McMullan, M. D. Miller, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, C. Puckett, R. Reyes, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The first structures from the FmdE Pfam family (PF02663) reveal that some members of this family form tightly intertwined dimers consisting of two domains (N-terminal [alpha]+[beta] core and C-terminal zinc-finger domains), whereas others contain only the core domain. The presence of the zinc-finger domain suggests that some members of this family may perform functions associated with transcriptional regulation, protein-protein interaction, RNA binding or metal-ion sensing.

PDB references: 2glz, 2gvi and 3d00

Online 4 August 2010


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Acta Cryst. (2010). F66, 1347-1353  [ doi:10.1107/S1744309109021988 ]

Conformational changes associated with the binding of zinc acetate at the putative active site of XcTcmJ, a cupin from Xanthomonas campestris pv. campestris

H. L. Axelrod, P. Kozbial, D. McMullan, S. S. Krishna, M. D. Miller, P. Abdubek, C. Acosta, T. Astakhova, D. Carlton, J. Caruthers, H.-J. Chiu, T. Clayton, M. C. Deller, L. Duan, Y. Elias, J. Feuerhelm, S. K. Grzechnik, J. C. Grant, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, A. Kumar, D. Marciano, A. T. Morse, K. D. Murphy, E. Nigoghossian, L. Okach, S. Oommachen, J. Paulsen, R. Reyes, C. L. Rife, H. J. Tien, C. V. Trout, H. van den Bedem, D. Weekes, A. White, Q. Xu, C. Zubieta, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of an RmlC-type cupin with zinc acetate bound at the putative active site reveals significant differences from a previous structure without any bound ligand. The functional implications of the ligand-induced conformational changes are discussed.

PDB reference: 3h50

Online 27 October 2009


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Acta Cryst. (2010). F66, 1354-1364  [ doi:10.1107/S1744309110021214 ]

Structure of the [gamma]-D-glutamyl-L-diamino acid endopeptidase YkfC from Bacillus cereus in complex with L-Ala-[gamma]-D-Glu: insights into substrate recognition by NlpC/P60 cysteine peptidases

Q. Xu, P. Abdubek, T. Astakhova, H. L. Axelrod, C. Bakolitsa, X. Cai, D. Carlton, C. Chen, H.-J. Chiu, M. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, K. Ellrott, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, W. W. Lam, D. Marciano, M. D. Miller, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, C. Puckett, R. Reyes, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, A. Yeh, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of the highly specific [gamma]-D-glutamyl-L-diamino acid endopeptidase YkfC from Bacillus cereus in complex with L-Ala-[gamma]-D-Glu reveals the structural basis for the substrate specificity of NlpC/P60-family cysteine peptidases.

PDB reference: 3h41

Online 27 July 2010


NMR in a high-throughput environment


 

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Acta Cryst. (2010). F66, 1365-1366  [ doi:10.1107/S1744309110030320 ]

NMR in a crystallography-based high-throughput protein structure-determination environment

K. Wüthrich

Synopsis: As an introduction to three papers on comparisons of corresponding crystal and NMR solution structures determined by the Joint Center for Structural Genomics (JCSG), an outline is provided of the JCSG strategy for combined use of the two techniques. A special commentary addresses the potentialities of the concept of `reference crystal structures', which is introduced in the following three papers.

Online 30 September 2010


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Acta Cryst. (2010). F66, 1367-1380  [ doi:10.1107/S1744309110005890 ]

NMR structure of the protein NP_247299.1: comparison with the crystal structure

K. Jaudzems, M. Geralt, P. Serrano, B. Mohanty, R. Horst, B. Pedrini, M.-A. Elsliger, I. A. Wilson and K. Wüthrich

Synopsis: Comparison of the NMR and crystal structures of a protein determined using largely automated methods has enabled the interpretation of local differences in the highly similar structures. These differences are found in segments of higher B values in the crystal and correlate with dynamic processes on the NMR chemical shift timescale observed in solution.

PDB reference: 2kla

Online 6 July 2010


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Acta Cryst. (2010). F66, 1381-1392  [ doi:10.1107/S1744309110020956 ]

Comparison of NMR and crystal structures for the proteins TM1112 and TM1367

B. Mohanty, P. Serrano, B. Pedrini, K. Jaudzems, M. Geralt, R. Horst, T. Herrmann, M.-A. Elsliger, I. A. Wilson and K. Wüthrich

Synopsis: NMR structures of the proteins TM1112 and TM1367 solved by the JCSG in solution at 298 K could be superimposed with the corresponding crystal structures at 100 K with r.m.s.d. values of <1.0 Å for the backbone heavy atoms. For both proteins the structural differences between multiple molecules in the asymmetric unit of the crystals correlated with structural variations within the bundles of conformers used to represent the NMR solution structures. A recently introduced JCSG NMR structure-determination protocol, which makes use of the software package UNIO for extensive automation, was further evaluated by comparison of the TM1112 structure obtained using these automated methods with another NMR structure that was independently solved in another PSI center, where a largely interactive approach was applied.

PDB references: 2k9z and 2ka0

Online 7 August 2010


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Acta Cryst. (2010). F66, 1393-1405  [ doi:10.1107/S1744309110033658 ]

Comparison of NMR and crystal structures highlights conformational isomerism in protein active sites

P. Serrano, B. Pedrini, M. Geralt, K. Jaudzems, B. Mohanty, R. Horst, T. Herrmann, M.-A. Elsliger, I. A. Wilson and K. Wüthrich

Synopsis: Tools for systematic comparisons of NMR and crystal structures developed by the JCSG were applied to two proteins with known functions: the T. maritima anti-[sigma] factor antagonist TM1081 and the mouse [gamma]-glutamylamine cyclotransferase A2LD1 (gi:13879369). In an attempt to exploit the complementarity of crystal and NMR data, the combined use of the two structure-determination techniques was explored for the initial steps in the challenge of searching proteins of unknown functions for putative active sites.

PDB references: 2ka5 and 2kl2

Online 30 September 2010


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