Structural Biology and Crystallization Communications

Acta Cryst. (2010). F66, 1406  [ doi:10.1107/S1744309110043733 ]

Publishing structural genomics results: the JCSG Special Issue

M. S. Weiss and H. Einspahr

Online 30 October 2010

Acta Cryst. (2010). F66, 1407-1414  [ doi:10.1107/S174430911003246X ]

Structural analysis of Bacillus pumilus phenolic acid decarboxylase, a lipocalin-fold enzyme

A. Matte, S. Grosse, H. Bergeron, K. Abokitse and P. C. K. Lau

Synopsis: The crystal structure of phenolic acid decarboxylase from B. pumilus strain UI-670 has been determined and refined at 1.69 Å resolution. The enzyme is a dimer, with each subunit adopting a -barrel structure belonging to the lipocalin fold.

PDB reference: 3nad

Online 27 October 2010

Acta Cryst. (2010). F66, 1415-1420  [ doi:10.1107/S1744309110035475 ]

Unliganded structure of human bisphosphoglycerate mutase reveals side-chain movements induced by ligand binding

A. Patterson, N. C. Price and J. Nairn

Synopsis: The X-ray structure of human bisphosphoglycerate mutase was solved to 1.94 Å resolution and a bisphosphoglycerate mutase-citrate model was built to explore enzyme-inhibitor interactions.

PDB reference: 3nfy

Online 27 October 2010

Acta Cryst. (2010). F66, 1421-1425  [ doi:10.1107/S1744309110036286 ]

The homodimeric GBS1074 from Streptococcus agalactiae

A. Shukla, M. Pallen, M. Anthony and S. A. White

Synopsis: The homodimeric nature of the ESAT-6 homologue GBS1074 and the potential for fibre-like assemblies are revealed by the 2 Å resolution crystal structure.

PDB reference: 3o9o

Online 27 October 2010

Acta Cryst. (2010). F66, 1426-1431  [ doi:10.1107/S1744309110036997 ]

Structure of Plasmodium falciparum ADP-ribosylation factor 1

W. J. Cook, C. D. Smith, O. Senkovich, A. A. Holder and D. Chattopadhyay

Synopsis: The crystal structure of GDP-bound ARF1 GTPase of Plasmodium falciparum has been determined at 2.5 Å resolution and compared with the structures of mammalian ARF1s.

PDB reference: 3lrp

Online 27 October 2010

Acta Cryst. (2010). F66, 1432-1435  [ doi:10.1107/S1744309110037085 ]

Structure of dihydroorotase from Bacillus anthracis at 2.6 Å resolution

S. Mehboob, D. C. Mulhearn, K. Truong, M. E. Johnson and B. D. Santarsiero

Synopsis: A comparison is made between the structures of dihydroorotase from four different organisms, including B. anthracis dihydroorotase, and reveals substantial variations in the active site, dimer interface and overall tertiary structure.

PDB reference: 3mpg

Online 27 October 2010

Acta Cryst. (2010). F66, 1436-1440  [ doi:10.1107/S1744309110039862 ]

Structure of the Francisella tularensis enoyl-acyl carrier protein reductase (FabI) in complex with NAD⁺ and triclosan

S. Mehboob, K. Truong, B. D. Santarsiero and M. E. Johnson

Synopsis: Structure of the ternary complex of F. tularensis enoyl-acyl carrier protein reductase reveals the structure of the substrate binding loop whose electron density was missing in an earlier structure, and demonstrates a shift in the position of the NAD⁺ cofactor.

PDB reference: 3nrc

Online 27 October 2010

Acta Cryst. (2010). F66, 1441-1444  [ doi:10.1107/S1744309110029192 ]

Crystallization and preliminary X-ray diffraction analysis of the fructofuranosidase from Xanthophyllomyces dendrorhous

A. Polo, D. Linde, M. Estévez, M. Fernández-Lobato and J. Sanz-Aparicio

Synopsis: The invertase from X. dendrorhous has been purified, deglycosylated and crystallized and diffraction data have been collected to 2.3 Å resolution.

Online 28 October 2010

Acta Cryst. (2010). F66, 1445-1448  [ doi:10.1107/S1744309110032136 ]

Crystallization and preliminary X-ray structural analysis of nucleoside triphosphate hydrolases from Neospora caninum and Toxoplasma gondii

K. Matoba, T. Shiba, T. Takeuchi, L. D. Sibley, M. Seiki, F. Kikyo, T. Horiuchi, T. Asai and S. Harada

Synopsis: Recombinant nucleoside triphosphate hydrolases from N. caninum and T. gondii have been purified and crystallized for X-ray structure analysis.

Online 28 October 2010

Acta Cryst. (2010). F66, 1449-1452  [ doi:10.1107/S1744309110032422 ]

Crystallization and preliminary X-ray analysis of a marine diatom-infecting single-stranded RNA virus

H. Naitow, Y. Shirai, Y. Tomaru and K. Nagasaki

Synopsis: A marine diatom-infecting virus was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group P6₃22, with unit-cell parameters a = b = 448.67, c = 309.76 Å, and diffracted to 4.0 Å resolution.

Online 28 October 2010

Acta Cryst. (2010). F66, 1453-1457  [ doi:10.1107/S1744309110032343 ]

Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus

Z. Kovárová, J. Chmelar, M. Sanda, J. Brynda, M. Mares and P. Rezácová

Synopsis: Cleavage of the serpin IRS-2 from the hard tick I. ricinus by contaminating proteolytic activity mimicked the specific processing of the serpin by its target protease and resulted in a more stable form of the serpin which produced crystals that diffracted to 1.8 Å resolution.

Online 28 October 2010

Acta Cryst. (2010). F66, 1458-1462  [ doi:10.1107/S1744309110032483 ]

Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus

S. L. Martin, R. H. Guenther, T. L. Sit, P. D. Swartz, F. Meilleur, S. A. Lommel and R. B. Rose

Synopsis: Virions of red clover necrotic mosaic virus have been purified and crystallized. The space group was determined to be I23, with unit-cell parameter a = 377.8 Å. The crystals diffracted to 4 Å resolution.

Online 28 October 2010

Acta Cryst. (2010). F66, 1463-1465  [ doi:10.1107/S1744309110032677 ]

Production, crystallization and preliminary X-ray analysis of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus

J. A. Brito, N. Borges, H. Santos and M. Archer

Synopsis: The expression, purification, crystallization and preliminary X-ray diffraction analysis of CTP:inositol-1-phosphate cytidylyltransferase from A. fulgidus is described.

Online 28 October 2010

Acta Cryst. (2010). F66, 1466-1469  [ doi:10.1107/S1744309110032872 ]

Purification, crystallization and preliminary X-ray analysis of a fusion of the LIM domains of LMO2 and the LID domain of Ldb1

K. El Omari, C. Porcher and E. J. Mancini

Synopsis: The crystallization and preliminary diffraction analysis of LMO2 in complex with the LID domain of Ldb1 is described. A three-wavelength anomalous dispersion (MAD) data set was collected to 2.8 Å resolution at the Zn K edge.

Online 28 October 2010

Acta Cryst. (2010). F66, 1470-1472  [ doi:10.1107/S1744309110033191 ]

Purification, crystallization and preliminary X-ray analysis of the dissimilatory sulfite reductase from Desulfovibrio vulgaris Miyazaki F

H. Ogata, Y. Shomura, A. Goenka Agrawal, A. P. Kaur, W. Gärtner, Y. Higuchi and W. Lubitz

Synopsis: Dissimilatory sulfite reductase from Desulfovibrio vulgaris Miyazaki F was purified and crystallized. The crystals belonged to the space group P4₁2₁2 and diffracted to 3.7 Å resolution.

Online 28 October 2010

Acta Cryst. (2010). F66, 1473-1476  [ doi:10.1107/S1744309110034317 ]

Expression, purification, crystallization and preliminary crystallographic analysis of PA3885 (TpbA) from Pseudomonas aeruginosa PAO1

W. Yang, K. Li, Y. Bai, R. Zhou, W. Zhou and M. Bartlam

Synopsis: PA3885 (TpbA), a tyrosine phosphatase, may function as a balancing factor between biofilm formation and motility in the opportunistic pathogen P. aeruginosa. Here, the expression, purification, crystallization and preliminary crystallographic analysis of PA3885 from P. aeruginosa PAO1 are reported.

Online 28 October 2010

Acta Cryst. (2010). F66, 1477-1479  [ doi:10.1107/S1744309110034913 ]

Crystallization and preliminary X-ray analysis of dimeric and trimeric cytochromes c from horse heart

M. Taketa, H. Komori, Y. Hattori, S. Nagao, S. Hirota and Y. Higuchi

Synopsis: Dimeric and trimeric cytochromes c were purified and crystallized. X-ray diffraction data sets were collected to resolutions of 2.2 and 2.1 Å, respectively.

Online 28 October 2010

Acta Cryst. (2010). F66, 1480-1483  [ doi:10.1107/S1744309110034949 ]

Crystallization and preliminary X-ray diffraction studies of a terminal oxygenase of carbazole 1,9a-dioxygenase from Novosphingobium sp. KA1

T. Umeda, J. Katsuki, Y. Ashikawa, Y. Usami, K. Inoue, H. Noguchi, Z. Fujimoto, H. Yamane and H. Nojiri

Synopsis: The terminal oxygenase component (Oxy) of carbazole 1,9a-dioxygenase (CARDO) catalyzes dihydroxylation of the aromatic ring. The Oxy of CARDO from Novosphingobium sp. KA1 was crystallized and the crystals diffracted to a resolution of 2.1 Å.

Online 28 October 2010

Acta Cryst. (2010). F66, 1484-1486  [ doi:10.1107/S1744309110035074 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of 23S RNA m²G2445 methyltransferase RlmL from Escherichia coli

K.-T. Wang, L. Ma, J. Nan, X.-D. Su and L. Li

Synopsis: RlmL, a 23S rRNA m2G2445 methyltransferase from Escherichia coli, was expressed, purified and crystallized, and crystals diffracted to 2.2 Å.

Online 28 October 2010

Acta Cryst. (2010). F66, 1487-1489  [ doi:10.1107/S1744309110035669 ]

Expression, purification, crystallization and preliminary X-ray analysis of a truncated soluble domain of human glioma pathogenesis-related protein 1

N. Bonafé, B. Zhan, M. E. Bottazzi, O. A. Perez, R. A. Koski and O. A. Asojo

Synopsis: The expression, purification, crystallization and preliminary X-ray diffraction analysis of the soluble domain of human glioma pathogenesis-related protein 1 (sGLIPR1) is presented.

Online 28 October 2010

Acta Cryst. (2010). F66, 1490-1492  [ doi:10.1107/S1744309110036067 ]

A preliminary X-ray study of human nucleoside diphosphate kinase A under oxidative conditions

M.-S. Kim, J. Jeong, K.-J. Lee and D. H. Shin

Synopsis: In this study, human NDPK-A has been crystallized under oxidative conditions and X-ray data have been collected to 2.80 Å resolution using synchrotron radiation.

Online 28 October 2010

Acta Cryst. (2010). F66, 1493-1495  [ doi:10.1107/S1744309110036031 ]

Preliminary crystallography confirms that the archaeal DNA-binding and tryptophan-sensing regulator TrpY is a dimer

J. Cafasso, B. A. Manjasetty, E. A. Karr, K. Sandman, M. R. Chance and J. N. Reeve

Synopsis: TrpY was crystallized using the hanging-drop method with ammonium sulfate as the precipitant. The crystals belonged to the tetragonal space group P4₃2₁2 or P4₁2₁2, with unit-cell parameters a = b = 87, c = 147 Å, and diffracted to 2.9 Å resolution.

Online 28 October 2010

Acta Cryst. (2010). F66, 1496-1499  [ doi:10.1107/S1744309110036043 ]

Crystallization and initial X-ray diffraction analysis of the tellurite-resistance S-adenosyl-L-methionine transferase protein TehB from Escherichia coli

H. G. Choudhury and K. Beis

Synopsis: The E. coli TehB methyltransferase has been purified and crystallized in the presence of SAM and sinefungin. Diffraction data have been collected to 1.9 Å resolution for both complexes.

Online 28 October 2010

Acta Cryst. (2010). F66, 1500-1502  [ doi:10.1107/S1744309110036481 ]

Purification, crystallization and preliminary X-ray analysis of 3-hydroxy-3-methylglutaryl-coenzyme A reductase of Streptococcus pneumoniae

L. Zhang, L. Feng, L. Zhou, J. Gui, J. Wan and X. Hu

Synopsis: 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase of Streptococcus pneumoniae has been cloned, overexpressed and purified to homogeneity using Ni-NTA affinity chromatography. Crystals were obtained using the hanging-drop vapour-diffusion method.

Online 29 October 2010

Acta Cryst. (2010). F66, 1503-1507  [ doi:10.1107/S1744309110036535 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding region of the Streptococcus gordonii adhesin GspB

T. M. Pyburn, V. Yankovskaya, B. A. Bensing, G. Cecchini, P. M. Sullam and T. M. Iverson

Synopsis: The carbohydrate-binding region of GspB from S. gordonii strain M99 was crystallized in space group P2₁2₁2₁ and data were collected to 1.3 Å resolution.

Online 29 October 2010

Acta Cryst. (2010). F66, 1508-1510  [ doi:10.1107/S1744309110036808 ]

Crystallization and preliminary X-ray analysis of a novel dye-linked L-proline dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix

T. Satomura, H. Sakuraba, Y. Hara and T. Ohshima

Synopsis: A novel dye-linked L-proline dehydrogenase from a hyperthermophilic archaeon was successfully isolated and crystallized.

Online 29 October 2010

Acta Cryst. (2010). F66, 1511-1516  [ doi:10.1107/S1744309110036791 ]

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the psychrophile Shewanella benthica

J. M. Wubben, C. Dogovski, R. C. J. Dobson, R. Codd, J. A. Gerrard, M. W. Parker and M. A. Perugini

Synopsis: Dihydrodipicolinate synthase (DHDPS) is an essential oligomeric enzyme of interest to antibiotic discovery research and studies probing the importance of quaternary structure to protein function, stability and dynamics. The cloning, expression, purification and crystallization of DHDPS from the psychrophilic (cold-dwelling) bacterium Shewanella benthica are described.

Online 29 October 2010

Acta Cryst. (2010). F66, 1517-1520  [ doi:10.1107/S1744309110036894 ]

Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356

Sakshi, D. N. Patil, S. Tomar, M. Sylvestre and P. Kumar

Synopsis: A 29 kDa cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from P. pnomenusa B-356 was purified, crystallized and characterized by the X-ray diffraction method.

Online 29 October 2010

Acta Cryst. (2010). F66, 1521-1524  [ doi:10.1107/S1744309110037346 ]

Crystallization and preliminary X-ray diffraction analysis of human cytosolic seryl-tRNA synthetase

J.-B. Artero, S. C. M. Teixeira, E. P. Mitchell, M. A. Kron, V. T. Forsyth and M. Haertlein

Synopsis: The crystallization and preliminary X-ray analysis of recombinant human cytosolic seryl-tRNA synthetase (hsSerRS) is reported. Diffraction data were collected to 3.13 Å resolution and the hsSerRS crystal structure is currently being refined and analyzed.

Online 29 October 2010

Acta Cryst. (2010). F66, 1525-1527  [ doi:10.1107/S1744309110037644 ]

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of Rv1698, an outer membrane channel protein from Mycobacterium tuberculosis

L. Chen, D. Sun, M. Wu, J. Zang and C. Tian

Synopsis: The outer membrane channel protein Rv1698 from M. tuberculosis has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.5 Å resolution.

Online 29 October 2010

Acta Cryst. (2010). F66, 1528-1530  [ doi:10.1107/S174430911003753X ]

Crystallization and X-ray diffraction studies of DNA-free and DNA-bound forms of EcoO109I DNA methyltransferase

M. Iwamoto, A. Hishiki, T. Shimada, T. Imasaki, J. Tsuda, K. Kita, T. Shimizu, M. Sato and H. Hashimoto

Synopsis: The crystallization and diffraction studies of EcoO109I DNA methyltransferase are described.

Online 29 October 2010

Acta Cryst. (2010). F66, 1531-1532  [ doi:10.1107/S1744309110037759 ]

Crystallization and preliminary crystallographic analysis of Achromobacter protease I mutants

L. Ito, K. Shiraki, T. Uchida, M. Okumura and H. Yamaguchi

Synopsis: Achromobacter protease I (API), a lysine-specific serine protease, exhibits an order of magnitude higher activity than bovine trypsin, while its optimum pH is in the alkaline region at approximately pH 9. Two single point mutants have previously been reported to improve the optimum pH range; determination of the tertiary structure of API mutants would help in understanding these mechanisms.

Online 29 October 2010

Acta Cryst. (2010). F66, 1533-1535  [ doi:10.1107/S1744309110037954 ]

Crystallization and preliminary crystallographic analysis of the type III secretion translocator chaperone SicA from Salmonella enterica

A. Priyadarshi and L. Tang

Synopsis: The SicA protein from the motile enterobacterium S. enterica serovar Typhimurium was overexpressed and crystallized. The crystals diffracted to 3.5 Å resolution and belonged to the monoclinic space group C2, with unit-cell parameters a = 180.4, b = 94.1, c = 131.8 Å, = 130.9°. The crystallographic asymmetric unit may contain as many as eight molecules, suggesting a unique oligomerization state.

Online 29 October 2010

Acta Cryst. (2010). F66, 1536-1540  [ doi:10.1107/S1744309110038698 ]

Crystallization, preliminary X-ray diffraction studies and Raman microscopy of the major haemoglobin from the sub-Antarctic fish Eleginops maclovinus in the carbomonoxy form

A. Merlino, L. Vitagliano, A. Balsamo, F. P. Nicoletti, B. D. Howes, D. Giordano, D. Coppola, G. di Prisco, C. Verde, G. Smulevich, L. Mazzarella and A. Vergara

Synopsis: The major haemoglobin of the sub-Antarctic fish E. maclovinus is the first sub-Antarctic fish haemoglobin to be crystallized and its structural characterization will shed light on the molecular mechanisms of cold adaptation and the role of the Root effect in fish.

Online 29 October 2010

Acta Cryst. (2010). F66, 1541-1543  [ doi:10.1107/S1744309110038832 ]

Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri

A. Pomowski, W. G. Zumft, P. M. H. Kroneck and O. Einsle

Synopsis: The physiologically active form of nitrous oxide reductase was isolated and crystallized under strict exclusion of dioxygen and diffraction data were collected from crystals belonging to two different space groups.

Online 29 October 2010

Acta Cryst. (2010). F66, 1544-1548  [ doi:10.1107/S1744309110039059 ]

Crystallization and preliminary X-ray diffraction analysis of the protease from Southampton norovirus complexed with a Michael acceptor inhibitor

R. J. Hussey, L. Coates, R. S. Gill, J. N. Wright, M. Sarwar, S. Coker, P. T. Erskine, J. B. Cooper, S. Wood, I. N. Clarke, P. R. Lambden, R. Broadbridge and P. M. Shoolingin-Jordan

Synopsis: The crystallization of the recombinant protease from Southampton norovirus is described. Whilst the native crystals were found to diffract only to medium resolution (2.9 Å), cocrystals with a designed covalently bound inhibitor diffracted X-rays to 1.7 Å resolution.

Online 29 October 2010

Acta Cryst. (2010). F66, 1549  [ doi:10.1107/S1744309110040972 ]

Crystallization and preliminary X-ray analysis of Na-ASP-1, a multi-domain pathogenesis-related-1 protein from the human hookworm parasite Necator americanus. Corrigendum

O. A. Asojo, A. Loukas, M. Inan, R. Barent, J. Huang, B. Plantz, A. Swanson, M. Gouthro, M. M. Meagher and P. J. Hotez

Synopsis: A correction to the paper by Asojo et al. [(2005), Acta Cryst. F61, 391-394].

Online 30 October 2010