Structural Biology and Crystallization Communications

Acta Cryst. (2011). F67, 178-181  [ doi:10.1107/S1744309110047482 ]

Structure of GlnK1, a signalling protein from Archaeoglobus fulgidus

C. Litz, S. Helfmann, S. Gerhardt and S. L. A. Andrade

Synopsis: The structure of GlnK1, one of three GlnK proteins encoded by the hyperthermophilic archaeon A. fulgidus, was solved to 2.28 Å resolution.

PDB reference: 3o8w

Online 21 January 2011

Acta Cryst. (2011). F67, 182-187  [ doi:10.1107/S1744309110048414 ]

Structure of the putative dihydroorotate dehydrogenase from Streptococcus mutans

Y. Liu, Z.-Q. Gao, C.-P. Liu, J.-H. Xu, L.-F. Li, C.-N. Ji, X.-D. Su and Y.-H. Dong

Synopsis: The crystal structure of SMU.595, a putative dihydroorotate dehydrogenase (DHOD) from S. mutans, is reported at 2.4 Å resolution.

PDB reference: 3oix

Online 21 January 2011

Acta Cryst. (2011). F67, 188-192  [ doi:10.1107/S1744309110049390 ]

Structure of the catalytic domain of glucoamylase from Aspergillus niger

J. Lee and M. Paetzel

Synopsis: A crystal structure of the catalytic domain of glucoamylase from A. niger with Tris and glycerol bound in the active site has been solved by molecular replacement and refined to 1.9 Å resolution.

PDB reference: 3eqa

Online 21 January 2011

Acta Cryst. (2011). F67, 193-200  [ doi:10.1107/S1744309110051250 ]

The structure of a haemopexin-fold protein from cow pea (Vigna unguiculata) suggests functional diversity of haemopexins in plants

V. Gaur, V. Chanana, A. Jain and D. M. Salunke

Synopsis: The structure of CP4, a haemopexin-fold protein from cow pea (Vigna unguiculata), has been determined at 2.1 Å resolution.

PDB reference: 3oyo

Online 21 January 2011

Acta Cryst. (2011). F67, 201-208  [ doi:10.1107/S1744309110052334 ]

Structure of the dimeric form of CTP synthase from Sulfolobus solfataricus

I. Lauritsen, M. Willemoës, K. F. Jensen, E. Johansson and P. Harris

Synopsis: The crystal structure of an unliganded form of CTP synthase from S. solfataricus unambiguously establishes that while the active form of the enzyme is tetrameric, a dimeric form has been crystallized. A comparison with the tetrameric form of the E. coli enzyme suggests that rather large intramolecular movements accompany tetramerization.

PDB reference: 3nva

Online 21 January 2011

Acta Cryst. (2011). F67, 209-213  [ doi:10.1107/S1744309110053030 ]

Structure of flap endonuclease 1 from the hyperthermophilic archaeon Desulfurococcus amylolyticus

T. Mase, K. Kubota, K. Miyazono, Y. Kawarabayasi and M. Tanokura

Synopsis: The crystal structure of flap endonuclease 1 from the hyperthermophilic archaeon D. amylolyticus was determined at 2.00 Å resolution.

PDB reference: 3ory

Online 21 January 2011

Acta Cryst. (2011). F67, 214-216  [ doi:10.1107/S1744309110048827 ]

Crystallization and preliminary X-ray crystallographic studies of enoyl-acyl carrier protein reductase (FabI) from Psuedomonas aeruginosa

J. H. Lee, A. K. Park, Y. M. Chi, J. H. Moon and K. S. Lee

Synopsis: Enoyl-acyl carrier protein reductase (FabI) from P. aeruginosa was purified and crystallized. FabI was also cocrystallized with the inhibitor triclosan and the cofactor NADH. Crystals of native and complexed FabI diffracted to resolutions of 2.6 and 1.8 Å, respectively.

Online 22 January 2011

Acta Cryst. (2011). F67, 217-220  [ doi:10.1107/S1744309110049067 ]

Crystallization and preliminary crystallographic analysis of -mannanase from Bacillus licheniformis

C. Songsiriritthigul, S. Lapboonrueng, S. Roytrakul, D. Haltrich and M. Yamabhai

Synopsis: The expression, purification, crystallization and preliminary crystallographic analysis of recombinant -mannanase from B. licheniformis strain DSM13 are described.

Online 22 January 2011

Acta Cryst. (2011). F67, 221-223  [ doi:10.1107/S1744309110050323 ]

Crystallization and preliminary X-ray analysis of the human long myosin light-chain kinase 1-specific domain IgCAM3

W. V. Graham, A. T. Magis, K. M. Bailey, J. R. Turner and D. A. Ostrov

Synopsis: To gain insight into the structure of the IgCAM3 domain, the IgCAM3 domain of MLCK1 has been expressed, purified and crystallized.

Online 22 January 2011

Acta Cryst. (2011). F67, 224-227  [ doi:10.1107/S1744309110050219 ]

Purification, crystallization and preliminary X-ray analysis of a thermostable direct haemolysin from Grimontia hollisae

Y.-K. Wang, S.-C. Huang, Y.-F. Wu, Y.-C. Chen, W.-H. Chen, Y.-L. Lin, M. Nayak, Y. R. Lin, T.T.-H. Li and T.-K. Wu

Synopsis: The thermostable direct haemolysin from G. hollisae has been purified and crystallized in two crystal forms using the vapour-diffusion method.

Online 22 January 2011

Acta Cryst. (2011). F67, 228-230  [ doi:10.1107/S1744309110050773 ]

Crystallization and preliminary crystallographic studies of a cysteine protease inhibitor from the human nematode parasite Ascaris lumbricoides

S. Liu, J. Dong, G. Mei, G. Liu, W. Xu, Z. Su and J. Liu

Synopsis: A recombinant cysteine protease inhibitor from the human nematode parasite A. lumbricoides has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.1 Å resolution.

Online 22 January 2011

Acta Cryst. (2011). F67, 231-233  [ doi:10.1107/S1744309110050876 ]

Crystallization and preliminary X-ray crystallographic studies of -transaminase from Mesorhizobium sp. strain LUK

B. Kim, O. K. Park, J. Y. Bae, T. Jang, J. H. Yoon, K. H. Do, B.-G. Kim, H. Yun and H. H. Park

Synopsis: -Transaminase from Mesorhizobium sp. strain LUK was crystallized. The crystals were found to belong to the orthorhombic space group C222₁, with unit-cell parameters a = 90.91, b = 192.17, c = 52.75 Å. The crystals were obtained at 293 K and diffracted to a resolution of 2.5 Å.

Online 22 January 2011

Acta Cryst. (2011). F67, 234-236  [ doi:10.1107/S1744309110050931 ]

Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom

A. Ullah, P. O. de Giuseppe, M. T. Murakami, D. Trevisan-Silva, A. C. M. Wille, D. Chaves-Moreira, L. H. Gremski, R. B. da Silveira, A. Sennf-Ribeiro, O. M. Chaim, S. S. Veiga and R. K. Arni

Synopsis: Wild-type and H12A-mutant class II phospholipase D from L. intermedia venom were crystallized; the crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively.

Online 22 January 2011

Acta Cryst. (2011). F67, 237-240  [ doi:10.1107/S1744309110051006 ]

Crystallization, X-ray diffraction analysis and preliminary structure determination of the TIR domain from the flax resistance protein L6

T. Ve, S. Williams, E. Valkov, J. G. Ellis, P. N. Dodds and B. Kobe

Synopsis: This report describes the crystallization and preliminary structure determination of the TIR domain from a plant disease-resistance protein.

Online 22 January 2011

Acta Cryst. (2011). F67, 241-244  [ doi:10.1107/S1744309110051110 ]

Purification, crystallization and preliminary X-ray diffraction of a disulfide cross-linked complex between bovine poly(A) polymerase and a chemically modified 15-mer oligo(A) RNA

Q. Yang, F. Faucher, M. Coseno, J. Heckman and S. Doublié

Synopsis: A disulfide cross-linked complex between bovine poly(A) polymerase and a chemically modified RNA was crystallized. X-ray diffraction data were collected to 2.25 Å resolution from crystals that belonged to space group P2.

Online 22 January 2011

Acta Cryst. (2011). F67, 245-248  [ doi:10.1107/S1744309110051249 ]

Crystallization and preliminary crystallographic analysis of Arabidopsis thaliana EDS1, a key component of plant immunity, in complex with its signalling partner SAG101

S. Wagner, S. Rietz, J. E. Parker and K. Niefind

Synopsis: The central plant immune regulator EDS1 (enhanced disease susceptibility1) from A. thaliana has been crystallized for the first time. Crystals of EDS1 in complex with its signalling partner SAG101 (senescence-associated gene101) diffracted to 3.5 Å resolution.

Online 22 January 2011

Acta Cryst. (2011). F67, 249-252  [ doi:10.1107/S1744309110051407 ]

Crystallization and preliminary X-ray diffraction analysis of a Lys49-phospholipase A₂ complexed with caffeic acid, a molecule with inhibitory properties against snake venoms

P. S. Shimabuku, C. A. H. Fernandes, A. J. Magro, T. R. Costa, A. M. Soares and M. R. M. Fontes

Synopsis: Piratoxin I, a noncatalytic and myotoxic Lys49-phospholipase A₂ from B. pirajai venom, was cocrystallized with the inhibitor caffeic acid and a data set was collected to a resolution of 1.65 Å. The electron-density map unambiguously indicated that three inhibitor molecules interact with the C-terminus of the protein.

Online 22 January 2011

Acta Cryst. (2011). F67, 253-257  [ doi:10.1107/S1744309110051286 ]

Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands

A. Stsiapanava, R. Chaloupkova, A. Fortova, J. Brynda, M. S. Weiss, J. Damborsky and I. Kuta Smatanova

Synopsis: Crystals of the wild-type haloalkane dehalogenase DhaA derived from R. rhodochrous NCIMB 13064 and of its catalytically inactive variant DhaA13 were grown in the presence of various ligands and diffraction data were collected to high and atomic resolution.

Online 22 January 2011

Acta Cryst. (2011). F67, 258-262  [ doi:10.1107/S1744309110051298 ]

Preliminary joint neutron time-of-flight and X-ray crystallographic study of human ABO(H) blood group A glycosyltransferase

B. Schuman, S. Z. Fisher, A. Kovalevsky, S. N. Borisova, M. M. Palcic, L. Coates, P. Langan and S. V. Evans

Synopsis: Collection to 2.5 Å resolution of neutron diffraction data from crystals of the human ABO(H) blood group glycosyltransferase GTA, is presented with the preliminary joint refinement with the corresponding X-ray diffraction data.

Online 22 January 2011

Acta Cryst. (2011). F67, 263-265  [ doi:10.1107/S1744309110051511 ]

Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori

J. Zhang, X. Zhang, X. Mao, Q. Zou and D. Li

Synopsis: Octaprenyl pyrophosphate synthase from H. pylori has been expressed, purified and crystallized, and a diffraction data set was collected to 2.00 Å resolution.

Online 22 January 2011

Acta Cryst. (2011). F67, 266-268  [ doi:10.1107/S1744309110051754 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of Lmo0540 from Listeria monocytogenes

J.-H. Jeong and Y.-G. Kim

Synopsis: The crystallization of Lmo0540 from L. monocytogenes is reported.

Online 27 January 2011

Acta Cryst. (2011). F67, 269-273  [ doi:10.1107/S1744309110052346 ]

Purification, crystallization and preliminary crystallographic analysis of the SH2 domain of IL-2-inducible T-cell kinase

R. E. Joseph, N. D. Ginder, J. A. Hoy, J. C. Nix, R. B. Honzatko and A. H. Andreotti

Synopsis: Crystallization conditions are described for the cis- and trans-imide bond-containing SH2 domain of IL-2-inducible T-cell kinase.

Online 27 January 2011

Acta Cryst. (2011). F67, 274-276  [ doi:10.1107/S1744309110052772 ]

Crystallization and initial crystallographic analysis of the Streptococcus parasanguinis FW213 Fap1-NR adhesive domain at pH 5.0

J. A. Garnett, S. Ramboarina, W. Lee, C. Tagliaferri, W. Wu and S. Matthews

Synopsis: The adhesin fimbriae-associated protein 1 (Fap1) is a surface protein of Streptococcus parasanguinis FW213 and plays a major role in the formation of dental plaque in humans. Here, the adhesive domain Fap1-NR, which is activated by acidic pH, has been crystallized at pH 5.0 and diffraction data have been collected to 3.0 Å resolution.

Online 27 January 2011

Acta Cryst. (2011). F67, 277-279  [ doi:10.1107/S1744309110052188 ]

Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the C-terminal domain of the GyrA subunit of DNA gyrase from Staphylococcus aureus strain Mu50

T.-O. Kim, H. Y. Jung, S. Y. Lee, D.-W. Im, W. Shin and Y.-S. Heo

Synopsis: The C-terminal domain of the DNA gyrase GyrA from S. aureus strain Mu50 was cloned, overexpressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.80 Å.

Online 27 January 2011

Acta Cryst. (2011). F67, 280-282  [ doi:10.1107/S1744309110052942 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of the C-terminal cytoplasmic domain of FlhB from Aquifex aeolicus

V. A. Meshcheryakov, Y.-H. Yoon and F. A. Samatey

Synopsis: The cytoplasmic domain of FlhB from A. aeolicus has been purified and crystallized.

Online 27 January 2011

Acta Cryst. (2011). F67, 283-286  [ doi:10.1107/S174430911005075X ]

Preliminary joint X-ray and neutron protein crystallographic studies of endoxylanase II from the fungus Trichoderma longibrachiatum

A. Y. Kovalevsky, B. L. Hanson, S. Seaver, S. Z. Fisher, M. Mustyakimov and P. Langan

Synopsis: A fungal family 11 endoxylanase has been crystallized at pH 8.5 and room-temperature X-ray and neutron diffraction data have been collected. Joint X-ray/neutron refinement is under way; the structural results will aid in rational engineering of the enzyme.

Online 27 January 2011

Acta Cryst. (2011). F67, 287-290  [ doi:10.1107/S1744309110053170 ]

Expression, crystallization and preliminary X-ray analysis of the phosphoribosylglycinamide formyltransferase from Streptococcus mutans

F. Zhai, X. Liu, J. Ruan, J. Li, Z. Liu, Y. Hu and S. Li

Synopsis: Phosphoribosylglycinamide formyltransferase (PurN) from Streptococcus mutans was expressed in E. coli, purified and studied crystallographically.

Online 27 January 2011

Acta Cryst. (2011). F67, 291-295  [ doi:10.1107/S1744309110051481 ]

Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid

A. Laguerre, J. Wielens, M. W. Parker, C. J. H. Porter and M. J. Scanlon

Synopsis: Intestinal fatty-acid binding proteins from human and rat have been crystallized in complex with the fluorescent probe 11-(dansylamino)undecanoic acid. Diffraction data for the crystals were collected to 1.8 Å resolution (human) and 1.6 Å resolution (rat).

Online 27 January 2011