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Figure 1
Optimization of refolding conditions for nectin-1-EC and nectin-2-EC. (ad) Effects of varying (a) the L-arginine concentration, (b) the pH, (c) the GSSG:GSH ratio and (d) the protein concentration on the recovery of nectin-1-EC. (e) Effects of varying the GSSG:GSH ratio on the recovery of nectin-2-EC. Samples from small-scale refolding reactions were subjected to size-exclusion chromatography on a Superdex 200 10/300 GL column. Correctly folded proteins eluted between 15 and 16.5 ml. Aggregates eluted between 8 and 9.5 ml. The insets show close-up views of the elution profiles between 14 and 17 ml.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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