Structure of an essential GTPase, YsxC, from Thermotoga maritima

Apo and GDP-bound crystal structures of an essential GTPase, YsxC, from T. maritima were determined to maximal resolutions of 2.3 and 1.9 Å, respectively. Switch I in GDP–YsxC can adopt both an `open' and `closed' conformation, suggesting a mechanism for diffusion of GDP out of the nucleotide-binding pocket.

Structure of human R-state aquomethemoglobin at 2.0 Å resolution

The 2.0 Å resolution crystal structure of tetrameric (αβ)₂ R-state human adult aquomethemoglobin reveals axial water coordination at both the α and β heme sites.

High-resolution structure of the recombinant sweet-tasting protein thaumatin I

The structure of a recombinant form of the sweet-tasting protein thaumatin I was determined at 1.1 Å resolution and refined to an R_work of 9.1% and an R_free of 11.7%. Comparisons with plant thaumatin revealed the electron density of recombinant thaumatin I to be significantly improved, especially around Asn46 and Ser63.

Preliminary X-ray crystallographic studies of the catalytic subunit of Escherichia coli AHAS II with its cofactors

In this study, the catalytic subunit of E. coli AHAS II was cocrystallized with its cofactors Mg²⁺, FAD and ThDP using the sitting-drop vapour-diffusion method and the crystals diffracted to 2.80 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of Escherichia coli CheA P3 dimerization domain

The P3 dimerization domain of CheA from E. coli has been crystallized (space group P1, unit-cell parameters a = 59.271, b = 67.674, c = 82.815 Å, α = 77.568, β = 86.073, γ = 64.436°). Diffraction data to 2.80 Å resolution have been collected using synchrotron radiation.

Crystallization and preliminary X-ray analysis of geraniol de­hydrogenase from Backhousia citriodora (lemon myrtle)

A recombinant form of geraniol dehydrogenase from Backhousia citriodora has been overexpressed in Escherichia coli and purified and crystallized by the sitting-drop method using polyethylene glycol 3350 as a precipitant.

Expression, purification, crystallization and preliminary X-ray diffraction studies of phosphoglycerate kinase from methicillin-resistant Staphylococcus aureus MRSA252

The cloning, overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of phosphoglycerate kinase from S. aureus MRSA252 is reported.

Crystallization and preliminary X-ray analysis of Aspergillus oryzae catechol oxidase

Catechol oxidase from A. oryzae was crystallized by the hanging-drop vapour-diffusion method.

Crystallization and preliminary X-ray crystallographic analysis of Salmonella Typhimurium CueP

S. Typhimurium CueP has been crystallized. Native data were collected to 2.5 Å resolution.

Crystallization of the factor H-binding protein, FhbB, from the periopathogen Treponema denticola

Factor H-binding protein B from T. denticola was expressed, purified and crystallized. A native data set was collected to 1.8 Å resolution.

Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans

Crystals of argininosuccinate lyase from S. mutans were obtained and X-ray data were collected to 2.5 Å resolution in space group R3.

Crystallization and preliminary X-ray crystallographic analysis of di­hydrouridine synthase from Thermus thermophilus and its complex with tRNA

Crystals of dihydrouridine synthase from Thermus thermophilus and its complex with tRNA were obtained and X-ray diffraction data were collected to 1.70 and 3.51 Å resolution, respectively.

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of glucuronic acid de­hydrogenase from Chromohalobacter salexigens

Recombinant glucuronic acid dehydrogenase from the halophilic bacterium Chromohalobacter salexigens has been crystallized and X-ray diffraction data collected to a maximum resolution of 2.1 Å.

Cloning, expression, purification, crystallization and preliminary X-ray crystallo­graphic study of GK0767, the copper-containing nitrite reductase from Geobacillus kaustophilus

The Cu-containing nitrite reductase from G. kaustophilus has been overexpressed, purified and crystallized in space group R3. The crystals diffracted to 1.3 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of the human kindlin-2 PH domain

The PH domain of human kindlin-2 was expressed, purified and crystallized. A complete X-ray diffraction data set was obtained at 2.8 Å resolution.

Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of Spatholobus parviflorus

A galactose specific lectin was purified from the seeds of a tropical plant, Spatholobus parviflorus. Its X-ray crystallographic structure was solved by the molecular replacement method.

Crystallization and preliminary X-ray diffraction analysis of kanamycin-binding β-lactamase in complex with its ligand

Recombinant BlaKr has been cocrystallized with kanamycin. A complete data set has been collected to 1.67 Å resolution using synchrotron radiation.

Crystallization and preliminary crystallographic studies of Helicobacter pylori arginase

In this study, H. pylori arginase was purified and crystallized in complex with Mn²⁺ and a diffraction data set was collected to 2.2 Å resolution.

Crystallization and preliminary X-ray characterization of the glpX-encoded class II fructose-1,6-bis­phosphatase from Mycobacterium tuberculosis

The crystallization and preliminary X-ray crystallographic analysis of the glpX-encoded class II fructose-1,6-bisphosphatase from M. tuberculosis in the apo form is reported.

Purification, crystallization and X-ray characterization of a Kunitz-type trypsin inhibitor protein from the seeds of chickpea (Cicer arietinum)

The purification, characterization and crystallization of a trypsin inhibitor protein isolated from chickpea seeds are reported.

Crystallization and preliminary structural analysis of the Listeria monocytogenes Ca²⁺-ATPase LMCA1

The L. monocytogenes Ca²⁺-ATPase LMCA1 was crystallized in a Ca²⁺-free E2–AlF₄⁻ form. A complete data set extending to 4.3 Å resolution was collected and a molecular-replacement solution was obtained using sarcoplasmic reticulum Ca²⁺-ATPase as a search model.

Expression, crystallization and preliminary X-ray analysis of a ferric binding protein from Thermus thermophilus HB8

The cloning, expression, purification, crystallization and preliminary X-ray analysis of a ferric binding protein encoded by T. thermophilus HB8 in apo and iron-bound holo states are presented. Four different crystal forms were obtained.

Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli

An O-methyltransferase from the ubiquinone-biosynthesis pathway in Escherichia coli, UbiG, with an N-­terminal hexahistidine tag has been expressed and crystallized. Crystals grown by the hanging-drop vapour-diffusion method diffracted to 2.00 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of eIF5BΔN and the eIF5BΔN–eIF1AΔN complex

The eukaryotic translation initiation factor eIF5BΔN and the eIF5BΔN–eIF1AΔN complex from S. cerevisiae were crystallized. The crystals diffracted to maximum resolutions of 2.45 and 3.3 Å, respectively.