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Figure 1
(a) The 1H–15N HSQC spectrum (red) of iron-associated Mt-RubB. (b) Assigned 1H–15N HSQC spectrum (blue) of zinc-associated Mt-RubB collected at 293 K in NMR buffer (100 mM NaCl, 20 mM Tris–HCl, 1.0 mM DTT, pH 7.1) at a 1H resonance frequency of 600 MHz. The cross-peaks for the 21-residue tag are labeled in magenta and with an asterisk. The only assigned side-chain –NH2 resonances are indicated by a dashed horizontal line and the exchangeable ring resonances for Trp22 and Trp33 are identified with an `r' [Trp40(r) at 10.81 p.p.m. (1H) and 131.2 p.p.m. (15N) is not shown]. A photograph of the NMR tube containing the sample for the respective 1H–15N HSQC spectrum is shown as an inset.

ISSN: 2053-230X
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