Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 67, Part 12 (December 2011)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Structure of LsrB from Yersinia pestis complexed with autoinducer-2 (Kavanaugh et al., p. 1501).

structural communications


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Acta Cryst. (2011). F67, 1466-1472  [ doi:10.1107/S1744309111034671 ]

Structures of engineered Clostridium botulinum neurotoxin derivatives

G. Masuyer, P. Stancombe, J. A. Chaddock and K. R. Acharya

Synopsis: The crystal structures of engineered C. botulinum neurotoxin-SNARE derivatives have been and exhibit strong stability of the LHn fragment.

PDB references: 3zus, 3zur and 3zuq

Online 25 November 2011


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Acta Cryst. (2011). F67, 1473-1479  [ doi:10.1107/S1744309111035238 ]

Structure of hyperthermophilic [beta]-glucosidase from Pyrococcus furiosus

Y. Kado, T. Inoue and K. Ishikawa

Synopsis: Recombinant hyperthermophilic [beta]-glucosidase from P. furiosus was crystallized. The crystal structure was solved to a resolution of 2.35 Å.

PDB reference: 3apg

Online 25 November 2011


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Acta Cryst. (2011). F67, 1480-1484  [ doi:10.1107/S1744309111037432 ]

The 1.7 Å resolution structure of At2g44920, a pentapeptide-repeat protein in the thylakoid lumen of Arabidopsis thaliana

S. Ni, M. E. McGookey, S. L. Tinch, A. N. Jones, S. Jayaraman, L. Tong and M. A. Kennedy

Synopsis: The crystal structure of At2g44920, a pentapeptide repeat protein (PRP) from Arabidopsis thaliana, has been determined at 1.7 Å resolution. The structure represents the first PRP protein whose subcellular localization has been experimentally confirmed to be the thylakoid lumen of a plant species.

PDB reference: 3n90

Online 25 November 2011


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Acta Cryst. (2011). F67, 1485-1491  [ doi:10.1107/S1744309111039595 ]

Engineered tryptophan in the adenine-binding pocket of catalytic subunit A of A-ATP synthase demonstrates the importance of aromatic residues in adenine binding, forming a tool for steady-state and time-resolved fluorescence spectroscopy

V. S. Tadwal, M. S. S. Manimekalai and G. Grüber

Synopsis: The crystallographic structures of the subunit B mutants F427W and F508W of the Pyrococcus horikoshii OT3 of the A1AO ATP synthase reveal that the exact volume of the adenine ribose binding pocket is essential for ATP-/ADP-binding.

PDB references: 3sdz and 3se0

Online 25 November 2011


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Acta Cryst. (2011). F67, 1492-1497  [ doi:10.1107/S1744309111040267 ]

Therapeutic target-site variability in [alpha]1-antitrypsin characterized at high resolution

A. O. M. Patschull, L. Segu, M. P. Nyon, D. A. Lomas, I. Nobeli, T. E. Barrett and B. Gooptu

Synopsis: A new 1.8 Å resolution structure of [alpha]1-antitrypsin demonstrates structural variability within an allosteric site in the molecule.

PDB reference: 3ne4

Online 25 November 2011


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Acta Cryst. (2011). F67, 1498-1500  [ doi:10.1107/S1744309111038449 ]

A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase

M. Alahuhta, P. Chandrayan, I. Kataeva, M. W. W. Adams, M. E. Himmel and V. V. Lunin

Synopsis: Structural analysis of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15.

PDB reference: 3t9g

Online 25 November 2011


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Acta Cryst. (2011). F67, 1501-1505  [ doi:10.1107/S1744309111042953 ]

The structure of LsrB from Yersinia pestis complexed with autoinducer-2

J. S. Kavanaugh, L. Gakhar and A. R. Horswill

Synopsis: The structure of LsrB from Y. pestis complexed with autoinducer-2 (AI-2) has been determined at 1.75 Å resolution. Bound AI-2 adopts the (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran conformation, which is the same conformation as is observed in the S. typhimurium and S. meliloti LsrB-AI-2 structures.

PDB reference: 3t95

Online 29 November 2011


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Acta Cryst. (2011). F67, 1506-1510  [ doi:10.1107/S1744309111046616 ]

Structure of d(CGGGTACCCG)4 as a four-way Holliday junction

P. K. Mandal, S. Venkadesh and N. Gautham

Synopsis: The crystal structure of the DNA sequence d(CGGGTACCCG)4 as a four-way Holliday junction and its interaction with a calcium ion are described.

PDB reference: 3t8p

Online 29 November 2011


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Acta Cryst. (2011). F67, 1511-1515  [ doi:10.1107/S1744309111042965 ]

The structure of an N11A mutant of the G-protein domain of FeoB

M.-R. Ash, M. J. Maher, J. M. Guss and M. Jormakka

Synopsis: The structure of NFeoB from S. thermophilus harbouring an N11A mutation has been determined to 1.85 Å resolution.

PDB reference: 3tah

Online 29 November 2011


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Acta Cryst. (2011). F67, 1516-1518  [ doi:10.1107/S1744309111045131 ]

Crystallization and preliminary X-ray structure analysis of human ribosomal protein L30e

A. Kawaguchi, T. Ose, M. Yao and I. Tanaka

Synopsis: The purification, crystallization and X-ray structure analysis of human L30e are presented here.

PDB reference: 3vi6

Online 30 November 2011


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Acta Cryst. (2011). F67, 1519-1523  [ doi:10.1107/S1744309111046203 ]

Structure of the H107R variant of the extracellular domain of mouse NKR-P1A at 2.3 Å resolution

P. Kolenko, D. Rozbeský, O. Vanek, K. Bezouska, J. Hasek and J. Dohnálek

Synopsis: The crystal structure of the H107R variant of the extracellular domain of mouse NKR-P1A was determined using X-ray diffraction from a merohedrally twinned crystal.

PDB reference: 3t3a

Online 30 November 2011


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Acta Cryst. (2011). F67, 1524-1530  [ doi:10.1107/S1744309111046343 ]

Structure of the fucose mutarotase from Streptococcus pneumoniae in complex with L-fucose

M. A. Higgins and A. B. Boraston

Synopsis: SpFcsU is a fucose mutarotase from S. pneumoniae that links the harvesting of fucose from glycans by glycoside hydrolases to processing of the fucose monosaccharide by subsequent enzymes in a fucose-utilization pathway. Here, the decameric structure of SpFcsU in complex with fucose is described.

PDB reference: 4a34

Online 30 November 2011


crystallization communications


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Acta Cryst. (2011). F67, 1531-1533  [ doi:10.1107/S1744309111038401 ]

Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the periplasmic domain of outer membrane protein A from Acinetobacter baumannii

J. S. Park, W. C. Lee, S. Choi, K. J. Yeo, J. H. Song, Y.-H. Han, J. C. Lee, S. I. Kim, Y. H. Jeon, C. Cheong and H.-Y. Kim

Synopsis: The crystallization of the OmpA periplasmic domain from A. baumannii is described.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1534-1536  [ doi:10.1107/S1744309111038218 ]

Purification, crystallization and preliminary X-ray characterization of a haemagglutinin from the seeds of Jatropha curcas

D. N. Nair, C. G. Suresh and D. D. Singh

Synopsis: A novel haemagglutinin from Jatropha curcas seeds is purified and crystallized. X-ray diffraction data collected from the rod-shaped crystals were processed in the orthorhombic space group P212121 and the crystals diffracted to 2.8 Å resolution at 103 K.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1537-1541  [ doi:10.1107/S1744309111038395 ]

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine Vitis vinifera

S. C. Atkinson, C. Dogovski, J. Newman, R. C. J. Dobson and M. A. Perugini

Synopsis: Dihydrodipicolinate synthase from the common grapevine V. vinifera has been cloned, expressed, purified and crystallized in the presence of the substrate pyruvate by in-drop hexahistidine-tag cleavage. A diffraction data set has been collected to a resolution of 2.2 Å.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1542-1544  [ doi:10.1107/S1744309111038425 ]

Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans

N. Suzuki, Y.-M. Kim, Z. Fujimoto, M. Momma, H.-K. Kang, K. Funane, M. Okuyama, H. Mori and A. Kimura

Synopsis: S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P21.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1545-1547  [ doi:10.1107/S1744309111038875 ]

Crystallization and preliminary X-ray analysis of crinumin, a chymotrypsin-like glycosylated serine protease with thrombolytic and antiplatelet activity

K. A. Singh, M. V. Jagannadham, G. R. K. Rao and P. H. N. Celie

Synopsis: The crystallization and preliminary X-ray diffraction analysis of crinumin, a plant serine protease, is reported.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1548-1550  [ doi:10.1107/S1744309111040590 ]

Crystallization and preliminary X-ray crystallographic analysis of [beta]-ketoacyl-ACP synthase I (XoFabB) from Xanthomonas oryzae pv. oryzae

T. T. N. Doan, J.-K. Kim, Q.-K. Mac, C. Chung, N. Sampath, J.-G. Kim, Y.-J. Ahn and L.-W. Kang

Synopsis: [beta]-Ketoacyl-acyl carrier protein synthase I (XoFabB) from X. oryzae pv. oryzae (Xoo) plays a crucial role in fatty-acid synthesis and has been considered as a target for the development of antibacterial agents against Xoo. XoFabB was expressed, purified and crystallized to determine its atomic resolution structure.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1551-1555  [ doi:10.1107/S174430911104108X ]

Overexpression, purification, crystallization and preliminary crystallographic studies of a hyperthermophilic adenylosuccinate synthetase from Pyrococcus horikoshii OT3

X. Wang, R. Akasaka, C. Takemoto, S. Morita, M. Yamaguchi, T. Terada, M. Shirozu, S. Yokoyama, S. Chen, S. Si and Y. Xie

Synopsis: A hyperthermophilic adenylosuccinate synthetase from P. horikoshii OT3, which is 90-120 amino acids shorter than those from the vast majority of organisms, was expressed, purified and crystallized and X-ray diffraction data were collected to 2.5 Å resolution.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1556-1558  [ doi:10.1107/S1744309111041194 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Archaeoglobus fulgidus

H. H. Lee

Synopsis: Shikimate dehydrogenase from A. fulgidus has been overexpressed and crystallized. X-ray diffraction data have been collected to 2.8 Å resolution.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1559-1562  [ doi:10.1107/S1744309111041145 ]

Crystallization and preliminary crystallographic analysis of a putative glucokinase/hexokinase from Thermus thermophilus

T. Nakamura, Y. Kashima, S. Mine, T. Oku and K. Uegaki

Synopsis: In this study, a putative glucokinase/hexokinase from T. thermophilus was purified and crystallized. Diffraction data were collected and processed to 2.02 Å resolution.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1563-1565  [ doi:10.1107/S1744309111039005 ]

Crystallization and preliminary X-ray crystallographic studies of VibE, a vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase from Vibrio cholerae

X. Liu, Z. Wang, D. Zhu, T. Wei, L. Gu and S. Xu

Synopsis: This article reports the molecular cloning, protein expression and purification, crystallization and preliminary X-ray crystallographic analysis of the vibriobactin synthetase VibE from V. cholerae.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1566-1569  [ doi:10.1107/S1744309111038383 ]

Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-T[Psi]C domain of tRNA

K. Ito, H. Qi, Y. Shimizu, R. Murakami, K. Miura, T. Ueda and T. Uchiumi

Synopsis: Peptidyl-tRNA hydrolase from E. coli has been crystallized in complex with the acceptor-T[Psi]C domain of tRNA. Diffraction data have been collected and processed to 2.4 Å resolution.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1570-1574  [ doi:10.1107/S1744309111040462 ]

Improved X-ray diffraction from Bacillus megaterium penicillin G acylase crystals through long cryosoaking dehydration

C. Rojviriya, T. Pratumrat, M. A. Saper and J. Yuvaniyama

Synopsis: Penicillin G acylase from the Gram-positive bacterium B. megaterium was crystallized and X-ray diffraction from these crystals could be substantially improved by slight dehydration through a long cryo-soak.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1575-1578  [ doi:10.1107/S1744309111040541 ]

Crystallization and preliminary crystallographic analysis of a C2 protein from Arabidopsis thaliana

M. Diaz, L. Rodriguez, M. Gonzalez-Guzman, M. Martínez-Ripoll and A. Albert

Synopsis: A C2 protein from A. thaliana has been crystallized by the hanging-drop vapour-diffusion method and a native data set has been collected at 2.4 Å resolution.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1579-1581  [ doi:10.1107/S1744309111040589 ]

Crystallization and preliminary crystallographic studies of the NAD+-dependent deacetylase HST1 from Saccharomyces cerevisiae

Y. Zhu, M. Teng and X. Li

Synopsis: The Saccharomyces cerevisiae NAD+-dependent deacetylase HST1156-503 was expressed and crystallized. Crystals grown by the hanging-drop vapour-diffusion method diffracted to 2.90 Å resolution.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1582-1585  [ doi:10.1107/S1744309111040413 ]

Expression, purification, crystallization and preliminary X-ray diffraction studies of the human keratin 4-binding domain of serine-rich repeat protein 1 from Streptococcus agalactiae

R. Sundaresan, U. Samen and K. Ponnuraj

Synopsis: Expression, purification and crystallization of Srr-1-K4BD, a human keratin 4-binding domain of serine-rich repeat protein 1 from S. agalactiae, was carried out. Native crystals of Srr-1-K4BD diffracted to 3.8 Å resolution using synchrotron radiation.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1586-1589  [ doi:10.1107/S1744309111040140 ]

Crystallization and preliminary X-ray analysis of crustacean hyperglycaemic hormone from the kuruma prawn Marsupenaeus japonicus in its weakly active precursor form

H. Inoue, N. Tsutsui, C. Nagai, K. Nagata, M. Tanokura and H. Nagasawa

Synopsis: Crustacean hyperglycaemic hormone from the kuruma prawn M. japonicus was crystallized by the sitting-drop vapour-diffusion method in its weakly active precursor form which has an extra glycine residue at the C-terminus. The crystals belonged to the orthorhombic space group P212121 and diffracted to 1.95 Å resolution.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1590-1594  [ doi:10.1107/S1744309111039960 ]

Expression, purification, crystallization and preliminary X-ray diffraction crystallographic study of PurH from Escherichia coli

X. Qiu, Y. Yuan and Y. Gao

Synopsis: Tthe cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of a bifunctional purine-biosynthesis enzyme from E. coli which possesses aminoimidazole-4-carboxamide ribonucleotide transformylase and IMP cyclohydrolase activities are reported.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1595-1598  [ doi:10.1107/S174430911103805X ]

Thermostable multicopper oxidase from Thermus thermophilus HB27: crystallization and preliminary X-ray diffraction analysis of apo and holo forms

H. Serrano-Posada, B. Valderrama, V. Stojanoff and E. Rudiño-Piñera

Synopsis: A thermostable multicopper oxidase from Thermus thermophilus HB27 (Tth-MCO) has been successfully crystallized using the sitting-drop and hanging-drop vapour-diffusion methods.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1599-1602  [ doi:10.1107/S1744309111038097 ]

Crystallization of Chlorella deoxyuridine triphosphatase

L. Badalucco, I. Poudel, M. Yamanishi, C. Natarajan and H. Moriyama

Synopsis: The crystallization of Chlorella dUTPase is described. The crystallization of plant dUTPase isozymes and their parameterization is discussed.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1603-1607  [ doi:10.1107/S1744309111037675 ]

Crystallization and X-ray diffraction analysis of the C-terminal domain of the flax rust effector protein AvrM

T. Ve, S. J. Williams, A. Stamp, E. Valkov, P. N. Dodds, P. A. Anderson and B. Kobe

Synopsis: This report describes the crystallization and X-ray diffraction analysis of two different allelic variants of the AvrM effector from flax rust.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1608-1611  [ doi:10.1107/S1744309111039741 ]

Crystallization and preliminary X-ray diffraction analysis of Val57 mutants of the amyloidogenic protein human cystatin C

M. Orlikowska, E. Jankowska, D. Borek, Z. Otwinowski, P. Skowron and A. Szymanska

Synopsis: The crystallization and preliminary X-ray diffraction analysis of Val57 mutants of human cystatin C, designed to assess the influence of changes in the properties of loop L1 on dimerization propensity, are reported.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1612-1615  [ doi:10.1107/S174430911103836X ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, Der f 7, from the dust mite Dermatophagoides farinae

K. W. Tan, S. Kumar, F. T. Chew and Y. K. Mok

Synopsis: The major group 7 allergen, Der f 7, from the dust mite Dermatophagoides farinae has been crystallized and diffracted X-rays to a resolution of 2.24 Å.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1616-1618  [ doi:10.1107/S1744309111038966 ]

Purification, crystallization and preliminary X-ray diffraction studies of the arsenic repressor ArsR from Corynebacterium glutamicum

S. Santha, E. P. J. Pandaranayaka, B. P. Rosen and S. Thiyagarajan

Synopsis: The cysteine free derivative of the arsenic repressor ArsR from Corynebacterium glutamicum was expressed, purified, crystallized and X-ray diffraction data up to 1.86 Å resolution have been collected. The crystals belonged to the space group P4 with the unit-cell parameters a = b = 41.84, c = 99.47 Å.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1619-1622  [ doi:10.1107/S1744309111039315 ]

Crystallization and X-ray data collection of HP0902 from Helicobacter pylori 26695

D.-W. Sim, J. H. Song, W. C. Lee, Y.-S. Lee, H.-Y. Kim and H.-S. Won

Synopsis: Two types of His6-tag-fused HP0902 protein from H. pylori 26695 have been crystallized and X-ray diffraction data were obtained at 1.4 and 2.5 Å resolution.

Online 26 November 2011


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Acta Cryst. (2011). F67, 1623-1626  [ doi:10.1107/S1744309111028995 ]

Cloning, purification and crystallographic analysis of a hypothetical protein, BPSL1549, from Burkholderia pseudomallei

A. Cruz-Migoni, S. N. Ruzheinikov, S. E. Sedelnikova, B. Obeng, S. Chieng, R. Mohamed, S. Nathan, P. J. Baker and D. W. Rice

Synopsis: B. pseudomallei BPSL1549 has been overexpressed in E. coli, purified and crystallized.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1627-1629  [ doi:10.1107/S174430911103291X ]

Purification, crystallization and preliminary X-ray diffraction of fluorescence recovery protein from Synechocystis PCC 6803

T. Liu, Y. Shuai and H. Zhou

Synopsis: Fluorescence recovery protein from Synechocystis PCC 6803 plays a key role in the orange carotenoid protein-related photoprotective mechanism in cyanobacteria. The full-length form and a truncated form were overexpressed, purified and crystallized, and diffraction was observed to 2.75 Å resolution.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1630-1632  [ doi:10.1107/S1744309111036165 ]

Crystallization and preliminary X-ray diffraction analysis of mouse prostaglandin F2[alpha] synthase, AKR1B3

Y. Takashima, S. Hatanaka, E. Mizohata, N. Nagata, Y. Fukunishi, H. Matsumura, Y. Urade and T. Inoue

Synopsis: Aldo-keto reductase 1B3 (AKR1B3) produced in Escherichia coli has been crystallized in complex with NADPH by the sitting-drop vapour-diffusion method.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1633-1636  [ doi:10.1107/S1744309111037390 ]

Expression, crystallization and preliminary crystallographic analysis of C-reactive protein from zebrafish

R. Chen, J. Qi, S. Yao, X. Pan, F. Gao and C. Xia

Synopsis: Crystals of native and selenomethionine-substituted C-reactive protein from zebrafish diffracted to 2.3 and 1.7 Å resolution, respectively, and belonged to space group R3 with one molecule per asymmetric unit. The Matthews coefficient was calculated to be 3.28 Å3 Da-1.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1637-1640  [ doi:10.1107/S1744309111038413 ]

Expression, purification and preliminary crystallographic analysis of Rv2247, the [beta] subunit of acyl-CoA carboxylase (ACCD6) from Mycobacterium tuberculosis

C. Niu, J. Yin, M. M. Cherney and M. N. G. James

Synopsis: The acyl-CoA carboxylase [beta] subunit (ACCD6) of M. tuberculosis has been crystallized and preliminary X-ray crystallographic analysis has been performed.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1641-1644  [ doi:10.1107/S1744309111039030 ]

Purification, crystallization and preliminary crystallographic analysis of peroxidase from the palm tree Chamaerops excelsa

L. C. Textor, J. C. Santos, N. Hidalgo Cuadrado, M. G. Roig, G. G. Zhadan, V. L. Shnyrov and I. Polikarpov

Synopsis: Diffraction-quality crystals of the peroxidase from the palm tree C. excelsa were obtained and a native X-ray diffraction data set was collected at a synchrotron source.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1645-1648  [ doi:10.1107/S1744309111041972 ]

Overexpression, purification, crystallization and preliminary X-ray analysis of CheY4 from Vibrio cholerae O395

M. Biswas, S. Khamrui, U. Sen and J. Dasgupta

Synopsis: The chemotaxis response regulator CheY4 from V. cholerae has been cloned, overexpressed, purified and crystallized in monoclinic and hexagonal space groups; the crystals diffracted to 1.67 and 1.9 Å resolution, respectively.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1649-1652  [ doi:10.1107/S174430911104231X ]

Crystallization and preliminary X-ray crystallographic analysis of a helicase-like domain from a tomato mosaic virus replication protein

H. Xiang, M. Niiyama, S. Sugiyama, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, M. Ishikawa, H. Matsumura and E. Katoh

Synopsis: Crystals of the helicase domain from a tomato mosaic virus replication protein obtained using the hanging-drop vapour-diffusion method at 285 K diffracted X-rays to 2.05 Å resolution. They belonged to the orthorhombic space group P212121, with unit-cell parameters a = 85.8, b = 128.3, c = 40.7 Å.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1653-1657  [ doi:10.1107/S1744309111043272 ]

Crystallization of a 79 kDa fragment of the hook protein FlgE from Campylobacter jejuni

Y. Kido, Y.-H. Yoon and F. A. Samatey

Synopsis: A 79 kDa fragment of FlgE from C. jejuni has been crystallized.

Online 30 November 2011


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Acta Cryst. (2011). F67, 1658-1661  [ doi:10.1107/S1744309111043740 ]

Preliminary X-ray crystallographic studies on acetate kinase (AckA) from Salmonella typhimurium in two crystal forms

S. Chittori, H. S. Savithri and M. R. N. Murthy

Synopsis: Acetate kinase from S. typhimurium was crystallized in two crystal forms that diffracted to 2.70 Å (form I) and 1.90 Å (form II) resolution.

Online 30 November 2011


laboratory communications


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Acta Cryst. (2011). F67, 1662-1665  [ doi:10.1107/S1744309111035718 ]

In-house SAD phasing with surface-bound cerium ions

K. N. Vennila and D. Velmurugan

Synopsis: Cerium was used to enhance the anomalous signal in hen egg-white lysozyme crystals and led to successful in-house SAD phasing.

Online 25 November 2011


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Acta Cryst. (2011). F67, 1666-1673  [ doi:10.1107/S1744309111042345 ]

Seleno-detergent MAD phasing of leukotriene C4 synthase in complex with dodecyl-[beta]-D-selenomaltoside

H. Saino, H. Ago, Y. Ukita and M. Miyano

Synopsis: Dodecyl-[beta]-D-selenomaltoside in a leukotriene C4 synthase crystal exhibited sufficient anomalous diffraction for multiwavelength anomalous diffraction phasing.

PDB reference: 3b29

Online 29 November 2011


addenda and errata


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Acta Cryst. (2011). F67, 1674  [ doi:10.1107/S1744309111050603 ]

Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate. Corrigendum

C. Ortíz, N. Larrieux, A. Medeiros, H. Botti, M. Comini and A. Buschiazzo

Synopsis: A correction to the article by Ortíz et al. [(2011), Acta Cryst. F67, 1457-1461].

Online 26 November 2011


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