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Figure 1
(a) SDS–PAGE analysis of AtDJ-1D at different steps of the purification process. Lane 1, marker (labelled in kDa); lane 2, total cell lysate; lane 3, supernatant after cell lysis; lane 4, pellet after cell lysis; lane 5, proteins from an Ni–NTA affinity column; lane 6, proteins separated after anion-exchange chromatography; lane 7, proteins separated after Superdex 200 chromatography (8.5 µg protein used). (b) Superdex 200 size-exclusion chromatography of AtDJ-1D. A single peak corresponding to a monomer was observed. The scale at the bottom indicates the elution volume. Inset, semi log plot of the molecular mass of the standard proteins used versus their logKd values.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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