Figure 1
Purification of recombinant BsLDH-WT and BsLDH-H171C. Lane M, molecular-weight standard (labeled in kDa); lane SN, supernatant fraction of cell lysate after centrifugation; lane FT, flowthrough fraction from Ni-NTA column; lane E1, eluate from Ni-NTA column; lane E2, eluate from ion-exchange chromatography.  [article HTML]

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