view article

Figure 1
SDS–PAGE analysis of β-Gal purification. Lane M, molecular markers (labelled in kDa); lane 1, culture supernatant; lane 2, β-Gal after Phenyl Sepharose purification; lane 3, β-Gal after Q Sepharose purification; lane 4, β-Gal after Endo Hf endoglycosidase treatment; lane 5, β-Gal after aminophenyl β-D-thiogalactopyranoside affinity purification; lane 6, β-Gal after limited trypsinization; lane 7, β-­Gal after Pros HS purification; lane 8, β-Gal after Superdex 200 gel filtration. Deglycosylated β-Gal precursor and mature β-Gal are indicated by arrows.

ISSN: 2053-230X
Follow Acta Cryst. F
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds