Sequence alignment of Y. pestis RipC with other citrate lyase β subunits (M. tuberculosis CitE, PDB entry 1u5h
; D. radiodurans, PDB entry 1sgj
; B. xenovorans, PDB entry 3r4i
; R. eutropha, PDB entry 3qqw
), as well as H. volcanii malate synthase H (PDB entry 3oyz
; Bracken et al., 2011). Cylinders and arrows define the regions of α-helices and β-strands, respectively. Dotted secondary-structure elements are predicted by JPred (Cole et al., 2008) and represent regions missing in the RipC crystal structure. RipC residues that are proposed to interact with CoA or a CoA derivative, based on sequence conservation with H. volcanii malate synthase H, are indicated by asterisks; fully conserved residues are boxed.