Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 68, Part 2 (February 2012)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Structure of Helicobacter pylori neutrophil-activating protein (HP-NAP) (Tsuruta et al., pp. 134).

structural communications


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Acta Cryst. (2012). F68, 115-118  [ doi:10.1107/S1744309111051219 ]

Structure of human tankyrase 1 in complex with small-molecule inhibitors PJ34 and XAV939

C. A. Kirby, A. Cheung, A. Fazal, M. D. Shultz and T. Stams

Synopsis: Crystal structures of human tankyrase I in complex with small-molecule inhibitors PJ34 and XAV939 have been determined, both to 2.0 Å. The TNKS1-PJ34 system allows for displacement soaking and, therefore, is an ideal system for structure-based drug design.

PDB references: 3uh2 and 3uh4

Online 21 January 2012


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Acta Cryst. (2012). F68, 119-123  [ doi:10.1107/S1744309111051499 ]

Room-temperature ultrahigh-resolution time-of-flight neutron and X-ray diffraction studies of H/D-exchanged crambin

J.C.-H. Chen, Z. Fisher, A. Y. Kovalevsky, M. Mustyakimov, B. L. Hanson, V. V. Zhurov and P. Langan

Synopsis: The 0.85 Å room-temperature ultrahigh-resolution structure of H/D-exchanged crambin is reported. Preliminary 1.1 Å resolution neutron diffraction data have been collected at the neutron Protein Crystallography Station at LANSCE.

PDB reference: 3u7t

Online 21 January 2012


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Acta Cryst. (2012). F68, 124-128  [ doi:10.1107/S1744309111052341 ]

Structures of new crystal forms of Mycobacterium tuberculosis peptidyl-tRNA hydrolase and functionally important plasticity of the molecule

M. Selvaraj, R. Ahmad, U. Varshney and M. Vijayan

Synopsis: The structures of new crystal forms of M. tuberculosis peptidyl-tRNA hydrolase confirm and provide further elaboration of the functionally relevant plasticity of the enzyme molecule.

PDB references: 3tck, 3td2, 3td6 and 3tcn

Online 21 January 2012


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Acta Cryst. (2012). F68, 129-133  [ doi:10.1107/S1744309111055400 ]

Structure of a pectin methylesterase from Yersinia enterocolitica

A. B. Boraston and D. W. Abbott

Synopsis: The first crystal structure of a pectin methylesterase from an enteropathogen is presented. This enzyme from Y. enterocolitica has biological significance for the evolution of pectin-metabolic pathways within pectinolytic bacteria and related agents of foodborne illness.

PDB reference: 3uw0

Online 21 January 2012


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Acta Cryst. (2012). F68, 134-140  [ doi:10.1107/S1744309111052675 ]

A new crystal lattice structure of Helicobacter pylori neutrophil-activating protein (HP-NAP)

O. Tsuruta, H. Yokoyama and S. Fujii

Synopsis: A new crystal lattice structure of H. pylori neutrophil-activating protein has been determined. Iron loading causes a series of conformational changes at the ferroxidase centre.

PDB references: 3t9j and 3ta8

Online 25 January 2012


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Acta Cryst. (2012). F68, 141-144  [ doi:10.1107/S1744309111049608 ]

Three-dimensional structure of a thermophilic family GH11 xylanase from Thermobifida fusca

A. Lammerts van Bueren, S. Otani, E. P. Friis, K. S. Wilson and G. J. Davies

Synopsis: The structure of a thermostable xylanase from T. fusca is reported.

PDB reference: 3zse

Online 25 January 2012


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Acta Cryst. (2012). F68, 145-153  [ doi:10.1107/S1744309111049761 ]

Structure of the interleukin-2 tyrosine kinase Src homology 2 domain; comparison between X-ray and NMR-derived structures

R. E. Joseph, N. D. Ginder, J. A. Hoy, J. C. Nix, D. B. Fulton, R. B. Honzatko and A. H. Andreotti

Synopsis: The interleukin-2 tyrosine kinase Src homology 2 domain was crystallized and its structure was solved to 2.35 Å resolution. The structure reveals a domain-swapped dimer that is related to other dimeric SH2 domains solved previously. The cis-trans-prolyl isomerization that is evident from solution studies of Itk SH2 cannot be observed in the crystal structure.

PDB reference: 3s9k

Online 25 January 2012


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Acta Cryst. (2012). F68, 154-158  [ doi:10.1107/S1744309111056132 ]

Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin-Arg-lysozyme complex

W. Liu, S. M. MacGrath, A. J. Koleske and T. J. Boggon

Synopsis: An unusual case of trace amounts of a contaminating protein facilitating formation of a heterotrimeric protein complex.

PDB reference: 3ulr

Online 25 January 2012


crystallization communications


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Acta Cryst. (2012). F68, 159-162  [ doi:10.1107/S1744309111049116 ]

Cloning, purification, crystallization and preliminary X-ray diffraction analysis of mouse PACSIN 3 protein

X. Bai, G. Meng and X. Zheng

Synopsis: The N-terminal F-BAR domain of mouse PACSIN 3, which contains 341 amino acids, has been successfully cloned, purified and crystallized.

Online 25 January 2012


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Acta Cryst. (2012). F68, 163-165  [ doi:10.1107/S1744309111050913 ]

Expression, purification, crystallization and preliminary crystallographic analysis of a thermostable DNA ligase from the archaeon Thermococcus sibiricus

T. E. Petrova, E. Y. Bezsudnova, B. D. Dorokhov, E. S. Slutskaya, K. M. Polyakov, P. V. Dorovatovskiy, N. V. Ravin, K. G. Skryabin, M. V. Kovalchuk and V. O. Popov

Synopsis: A recombinant ATP-dependent DNA ligase from the hyperthermophilic anaerobic archaeon Thermococcus sibiricus was expressed in Escherichia coli and purified. Crystals were grown by vapour diffusion using the hanging-drop method.

Online 25 January 2012


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Acta Cryst. (2012). F68, 166-171  [ doi:10.1107/S1744309111051049 ]

Crystallization of the C-terminal domain of the bacteriophage T7 fibre protein gp17

C. Garcia-Doval and M. J. van Raaij

Synopsis: The C-terminal domain of the bacteriophage T7 fibre protein gp17, consisting of amino acids 371-553, has been crystallized. Diffraction data have been obtained to around 2.0 Å resolution from two different crystal forms. Multiwavelength anomalous dispersion phasing with a mercury derivative is in progress.

Online 25 January 2012


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Acta Cryst. (2012). F68, 172-174  [ doi:10.1107/S1744309111051700 ]

Crystallization and preliminary X-ray diffraction analysis of the metalloregulatory protein DtxR from Thermoplasma acidophilum

H. K. Yeo, J. Kang, Y. W. Park, J.-S. Sung and J. Y. Lee

Synopsis: Orthorhombic crystals of DtxR from T. acidophilum have been obtained. X-ray data were collected to 1.8 Å resolution using synchrotron radiation.

Online 26 January 2012


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Acta Cryst. (2012). F68, 175-177  [ doi:10.1107/S1744309111051323 ]

Crystallization and preliminary crystallographic analysis of LipC12, a true lipase isolated through a metagenomics approach

V. P. Martini, A. Glogauer, J. Iulek, E. M. Souza, F. O. Pedrosa and N. Krieger

Synopsis: The preliminary X-ray analysis of LipC12, the first lipase isolated through a metagenomics approach to be crystallized, is reported.

Online 26 January 2012


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Acta Cryst. (2012). F68, 178-180  [ doi:10.1107/S1744309111051888 ]

Purification, crystallization and preliminary X-ray crystallographic studies of the Mycobacterium tuberculosis DNA gyrase CTD

A. Darmon, J. Piton, M. Roué, S. Petrella, A. Aubry and C. Mayer

Synopsis: The M. tuberculosis DNA gyrase A C-terminal domain (CTD) was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group P212121 and diffraction data were collected to a resolution of 1.55 Å.

Online 26 January 2012


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Acta Cryst. (2012). F68, 181-184  [ doi:10.1107/S1744309111052109 ]

Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa PelD

L. S. Marmont, J. C. Whitney, H. Robinson, K. M. Colvin, M. R. Parsek and P. L. Howell

Synopsis: The expression, purification and crystallization of a soluble construct of the putative inner membrane protein PelD from P. aeruginosa is described. The crystals diffracted to a resolution of 2.2 Å.

Online 26 January 2012


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Acta Cryst. (2012). F68, 185-189  [ doi:10.1107/S1744309111052110 ]

Crystallization of oligonucleotides containing A-rich repeats suggests a structural contribution to the autoregulation mechanism of PABP translation

K. Kikuchi, S. Shimizu, Y. Sato, E. C. Morishita and A. Takénaka

Synopsis: Several DNA and RNA fragments containing tandem AAAAAA repeats, which were synthesized as analogues of the A-rich repeats found in the autoregulatory sequence of PABP mRNA, have been crystallized, suggesting the possibility that the autoregulatory sequence has a specific structure which impedes the binding of PABP.

Online 26 January 2012


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Acta Cryst. (2012). F68, 190-192  [ doi:10.1107/S1744309111053164 ]

Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus: envelope protein ODV-E66

Y. Kawaguchi, N. Sugiura, M. Onishi, K. Kimata, M. Kimura and Y. Kakuta

Synopsis: Baculovirus envelope protein ODV-E66 (67-704) was crystallized; the crystal diffracted to 1.8 Å resolution and belonged to space group P62 or P64, with unit-cell parameters a = b = 113.5, c = 101.5 Å.

Online 26 January 2012


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Acta Cryst. (2012). F68, 193-195  [ doi:10.1107/S1744309111053723 ]

Crystallization and preliminary X-ray crystallographic analysis of dioscorin from Dioscorea japonica

Y.-L. Xue, T. Miyakawa, Y. Sawano and M. Tanokura

Synopsis: Dioscorin from D. japonica was expressed, purified and crystallized using the sitting-drop vapour-diffusion method. The dioscorin crystal diffracted X-rays to 2.11 Å resolution.

Online 26 January 2012


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Acta Cryst. (2012). F68, 196-198  [ doi:10.1107/S1744309111053942 ]

Purification, crystallization and preliminary X-ray characterization of Bacillus cereus arylamine N-acetyltransferase 3 [(BACCR)NAT3]

X. Kubiak, B. Pluvinage, I. Li de la Sierra-Gallay, P. Weber, A. Haouz, J.-M. Dupret and F. Rodrigues-Lima

Synopsis: B. cereus arylamine N-acetyltransferase 3 was expressed, purified and crystallized. X-ray diffraction data were collected to 2.42 Å resolution and the crystals belonged to the monoclinic space group C121.

Online 26 January 2012


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Acta Cryst. (2012). F68, 199-202  [ doi:10.1107/S1744309111054212 ]

Crystallization and preliminary X-ray analysis of S-ribosylhomocysteinase from Streptococcus mutans

H. Li, H. Zhao, L. Zhu, L. Hong, H. Zhang, F. Lin, C. Xu, S. Li and Z. Zhang

Synopsis: S-Ribosylhomocysteinase (LuxS) encoded by the LuxS gene from Streptococcus mutans was solubly expressed in Escherichia coli, purified and crystallized. Diffraction by the crystal extended to 2.4 Å resolution.

Online 26 January 2012


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Acta Cryst. (2012). F68, 203-206  [ doi:10.1107/S174430911105425X ]

Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase

T. Tsuda, T. Suzuki and S. Kojima

Synopsis: Native and selenomethionine-derivatized (SeMet) crystals of Bacillus subtilis YwfE in the presence of ADP, MgCl2 and the dipeptide L-Ala-L-Gln were obtained using the hanging-drop vapour-diffusion method.

Online 26 January 2012


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Acta Cryst. (2012). F68, 207-210  [ doi:10.1107/S1744309111054388 ]

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of aspartyl aminopeptidase from the apeB gene of Pseudomonas aeruginosa

S. Natarajan and R. Mathews

Synopsis: Aspartyl aminopeptidase (APP) is a metallopeptidase that catalyzes the hydrolysis of aspartic acid from the N-termini of polypeptide chains. The apeB gene from P. aeruginosa was cloned, the APP enzyme was expressed and crystallized and a preliminary X-ray crystallographic study was performed in order to understand its unique reaction pathway.

Online 26 January 2012


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Acta Cryst. (2012). F68, 211-213  [ doi:10.1107/S1744309111054923 ]

Crystallization and preliminary X-ray diffraction analysis of an L-amino-acid oxidase from Bothrops jararacussu venom

A. Ullah, M. Coronado, M. T. Murakami, C. Betzel and R. K. Arni

Synopsis: An L-amino-acid oxidase from B. jararacussu venom was crystallized and X-ray diffraction data were collected to a maximum resolution of 3.0 Å.

Online 26 January 2012


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Acta Cryst. (2012). F68, 214-217  [ doi:10.1107/S1744309111055230 ]

Crystallization and preliminary X-ray diffraction analysis of human dipeptidyl peptidase 10 (DPPY), a component of voltage-gated potassium channels

G. A. Bezerra, E. Dobrovetsky, A. Seitova, S. Dhe-Paganon and K. Gruber

Synopsis: The expression, purification and crystallization of human dipeptidyl peptidase 10, a component of voltage-gated potassium channels, is described.

Online 26 January 2012


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Acta Cryst. (2012). F68, 218-221  [ doi:10.1107/S1744309111055187 ]

Expression, purification and preliminary structural analysis of the coiled-coil domain of Deinococcus radiodurans RecN

S. Pellegrino, D. de Sanctis, S. McSweeney and J. Timmins

Synopsis: The coiled-coil domain of the essential DNA-repair protein RecN from D. radiodurans was crystallized. The crystals belonged to space group P21 and diffracted X-rays to 2.04 Å resolution.

Online 26 January 2012


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Acta Cryst. (2012). F68, 222-226  [ doi:10.1107/S1744309111055503 ]

Crystallization and X-ray diffraction analysis of a novel surface-adhesin protein: protein E from Haemophilus influenzae

B. Singh, T. Al Jubair, K. Förnvik, M. M. Thunnissen and K. Riesbeck

Synopsis: Protein E of the respiratory pathogen H. influenzae is a multifunctional adhesin that is involved in bacterial attachment to host epithelium and direct interactions with vitronectin, laminin and plasminogen. The method of crystallization and X-ray data collection for protein E at 1.8 Å is presented.

Online 27 January 2012


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Acta Cryst. (2012). F68, 227-230  [ doi:10.1107/S174430911105603X ]

Crystallization and preliminary X-ray analysis of the Clostridium botulinum type D nontoxic nonhaemagglutinin

K. Miyata, K. Inui, S.-I. Miyashita, Y. Sagane, K. Hasegawa, T. Matsumoto, A. Yamano, K. Niwa, T. Watanabe and T. Ohyama

Synopsis: The nontoxic nonhaemagglutinin of serotype D neurotoxin complex, which is important in proteolytic stability for toxin complex, was expressed in E. coli, and crystallized.

Online 27 January 2012


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Acta Cryst. (2012). F68, 231-235  [ doi:10.1107/S1744309111056028 ]

Refolding, purification and crystallization of the FrpB outer membrane iron transporter from Neisseria meningitidis

M. Saleem, S. M. Prince, H. Patel, H. Chan, I. M. Feavers and J. P. Derrick

Synopsis: The refolding, purification and crystallization of FrpB from the meningitis pathogen Neisseria meningitidis is described.

Online 27 January 2012


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Acta Cryst. (2012). F68, 236-239  [ doi:10.1107/S1744309111055928 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the Hsp40 protein CPIP1 from Nicotiana tabacum

M. H. Griessl, I. Jungkunz, U. Sonnewald and Y. A. Muller

Synopsis: CPIP1 from N. tabacum (NtCPIP1) is a plant heat-shock 40-type protein that participates in effector-host interactions during potyviral infections. Whereas crystals of full-length wild-type NtCPIP1 only diffracted to 8.0 Å resolution, diffraction from crystals of a shortened variant lacking the first 127 amino acids extended to 2.4 Å resolution.

Online 27 January 2012


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Acta Cryst. (2012). F68, 240-243  [ doi:10.1107/S1744309111041091 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of a ribokinase from the thermohalophile Halothermothrix orenii

L. D. Kori, A. Hofmann and B. K. C. Patel

Synopsis: A ribokinase gene (rbk) from the anaerobic halothermophilic bacterium Halothermothrix orenii was cloned and overexpressed in Escherichia coli. The recombinant protein (Ho-Rbk) was purified using immobilized metal-ion affinity chromatography and crystals were obtained using the sitting-drop method.

Online 27 January 2012


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Acta Cryst. (2012). F68, 244-246  [ doi:10.1107/S1744309111043247 ]

Crystallization and preliminary X-ray diffraction analysis of orotate phosphoribosyltransferase from the human malaria parasite Plasmodium falciparum

Y. Takashima, E. Mizohata, K. Tokuoka, S. R. Krungkrai, Y. Kusakari, S. Konishi, A. Satoh, H. Matsumura, J. Krungkrai, T. Horii and T. Inoue

Synopsis: Orotate phosphoribosyltransferase from Plasmodium falciparum produced in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method in complex with OA and PRPP in the presence of Mg2+.

Online 27 January 2012


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Acta Cryst. (2012). F68, 247-251  [ doi:10.1107/S1744309111042448 ]

Notes for authors 2012

Online 28 January 2012


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