Acta Cryst. (2012). F68, 501-510
[ doi:10.1107/S1744309112012857 ]

The precursor form of Hansenula polymorpha copper amine oxidase 1 in complex with Cu^I and Co^II

V. J. Klema, B. J. Johnson, J. P. Klinman and C. M. Wilmot

Synopsis: The structure of copper amine oxidase 1 from H. polymorpha in its metal-free precursor (apo) form is reported along with structures of the apo protein in complex with Cu^I and Co^II.

PDB references: 3sx1, 3sxx and 3t0u

Online 20 April 2012

 

Acta Cryst. (2012). F68, 511-516
[ doi:10.1107/S1744309112010226 ]

Structure of a hexameric form of RadA recombinase from Methanococcus voltae

L. Du and Y. Luo

Synopsis: Hexameric rings of RadA recombinase from M. voltae have been crystallized. Structural comparisons suggest that homologues of RadA tend to form double-ringed assemblies.

PDB reference: 4dc9

Online 20 April 2012

 

Acta Cryst. (2012). F68, 517-521
[ doi:10.1107/S1744309112011359 ]

New crystal structure of the proteasome-dedicated chaperone Rpn14 at 1.6 Å resolution

S. Kim, A. Nishide, Y. Saeki, K. Takagi, K. Tanaka, K. Kato and T. Mizushima

Synopsis: A new crystal structure of yeast Rpn14 with an E384A mutation was determined at 1.6 Å resolution. The improved high-resolution structure provides a framework for understanding proteasome assembly.

PDB reference: 3vl1

Online 20 April 2012

 

Acta Cryst. (2012). F68, 522-526
[ doi:10.1107/S1744309112011487 ]

The structure of an orthorhombic crystal form of a `forced reduced' thiol peroxidase reveals lattice formation aided by the presence of the affinity tag

K. S. H. Beckham, O. Byron, A. J. Roe and M. Gabrielsen

Synopsis: The crystal structure of the C61S mutant of Tpx from E. coli is reported at 1.97 Å resolution. A brief structural comparison with homologues is presented.

PDB reference: 4af2

Online 20 April 2012

 

Acta Cryst. (2012). F68, 527-534
[ doi:10.1107/S1744309112011037 ]

Structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form: insights into the path of carbamoyl phosphate to the active site of the enzyme

J. Vitali, A. K. Singh, A. S. Soares and M. J. Colaneri

Synopsis: The structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase has been determined in a hexagonal crystal form and gives insight into the possible paths that the substrate carbamoyl phosphate may follow to reach the active site during catalysis.

PDB reference: 4ekn

Online 20 April 2012

 

Acta Cryst. (2012). F68, 535-539
[ doi:10.1107/S1744309112009347 ]

Initial crystallographic studies of a small heat-shock protein from Xylella fastidiosa

S. F. S. Tada, A. M. Saraiva, G. S. Lorite, L. K. Rosselli-Murai, A. C. Pelloso, M. L. dos Santos, D. B. B. Trivella, M. A. Cotta, A. P. de Souza and R. Aparicio

Synopsis: Initial crystallographic studies of the X. fastidiosa small heat-shock protein HSP17.9 are reported.

Online 20 April 2012

 

Acta Cryst. (2012). F68, 540-542
[ doi:10.1107/S1744309112011645 ]

Expression, purification, crystallization and preliminary X-ray analysis of carbonyl reductase S1 from Candida magnoliae

Y. Suwa, J. Ohtsuka, T. Miyakawa, F. L. Imai, M. Okai, Y. Sawano, Y. Yasohara, M. Kataoka, S. Shimizu and M. Tanokura

Synopsis: Carbonyl reductase S1 from C. magnoliae was expressed, purified and crystallized. The crystals obtained diffracted X-rays to 1.90 Å resolution and belonged to space group P6₁22 or P6₅22.

Online 20 April 2012

 

Acta Cryst. (2012). F68, 543-546
[ doi:10.1107/S1744309112012031 ]

Crystallization and preliminary X-ray diffraction analysis of the SOD-TAT fusion protein

J. Pan, K. Zhou, G. Zheng, S. Liu and P. Rao

Synopsis: A cell membrane permeable SOD, SOD-TAT fusion protein, was expressed, purified and crystallized. X-ray diffraction data have been collected to 3.2 Å resolution.

Online 20 April 2012

 

Acta Cryst. (2012). F68, 547-550
[ doi:10.1107/S1744309112010421 ]

Expression, purification, crystallization and preliminary crystallographic studies of Rhagium inquisitor antifreeze protein

A. Hakim, D. Thakral, D. F. Zhu and J. B. Nguyen

Synopsis: A novel hyperactive antifreeze protein from R. inquisitor (RiAFP) has been overexpressed, purified and crystallized. A complete native X-ray diffraction data set was recorded to 1.3 Å resolution.

Online 20 April 2012

 

Acta Cryst. (2012). F68, 551-556
[ doi:10.1107/S1744309112011025 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of 3-ketosteroid ¹-dehydrogenase from Rhodococcus erythropolis SQ1

A. Rohman, N. van Oosterwijk and B. W. Dijkstra

Synopsis: 3-Ketosteroid ¹-dehydrogenase from Rhodococcus erythropolis SQ1 was successfully crystallized and its initial structure was solved.

Online 20 April 2012

 

Acta Cryst. (2012). F68, 557-559
[ doi:10.1107/S1744309112011256 ]

Crystallization and preliminary X-ray crystallographic analysis of the methionine sulfoxide reductase A domain of MsrAB from Haemophilus influenzae

A. R. Han, H. S. Kim, G. Y. Cho, H. S. Ki, H.-Y. Kim and K. Y. Hwang

Synopsis: The MsrA domain of MsrAB from H. influenzae was crystallized. X-ray diffraction data were collected to 1.6 Å resolution.

Online 20 April 2012

 

Acta Cryst. (2012). F68, 560-564
[ doi:10.1107/S1744309112009992 ]

Identification, crystallization and preliminary X-ray diffraction analysis of esterase A from Caulobacter crescentus CB15, a family VIII lipolytic enzyme

B. H. Ryu, T. D. Ngo, E. Jang, S. Kim, H. Ju, K. K. Kim and T. D. Kim

Synopsis: Esterase A from C. crescentus CB15 was crystallized in space group C222₁ and diffraction data were collected to a resolution of 1.62 Å.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 565-568
[ doi:10.1107/S1744309112009761 ]

Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases from Streptococcus mutans

C. Wang, X. Fan, X. Cao, X. Liu, L. Li and X. Su

Synopsis: Two hypothetical ribose-5-phosphate isomerases from S. mutans have been produced in E. coli and crystallized. The crystals diffracted to high resolutions suitable for crystallographic analyses.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 569-573
[ doi:10.1107/S1744309112009736 ]

Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein

S. D. Carter, J. N. Barr and T. A. Edwards

Synopsis: The Crimean-Congo haemorrhagic fever virus nucleocapsid protein was expressed in E. coli, purified and crystallized. X-ray diffraction data were collected to 2.1 Å resolution.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 574-579
[ doi:10.1107/S1744309112007890 ]

Crystallization and preliminary crystallographic study of a trypsin-resistant catalytic domain of human calcineurin

L. Jin, M. H. A. Roehrl, L. Xiao, X. He, H. Li, L. Ge and B. Shi

Synopsis: A trypsin-resistant catalytic domain of human calcineurin (A subunit, residues 20-347) was crystallized in space group P2₁2₁2. An X-ray diffraction data set was collected to 2.87 Å resolution and the structure was solved by molecular replacement.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 580-583
[ doi:10.1107/S1744309112010330 ]

Crystallization and preliminary crystallographic analysis of the major capsid proteins VP16 and VP17 of bacteriophage P23-77

I. Rissanen, A. Pawlowski, K. Harlos, J. M. Grimes, D. I. Stuart and J. K. H. Bamford

Synopsis: The major capsid proteins VP16 and VP17 of bacteriophage P23-77 have been crystallized using both recombinant and purified virus and preliminary diffraction analyses have been performed.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 584-587
[ doi:10.1107/S1744309112012808 ]

Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgL

F. Wolfram, K. Arora, H. Robinson, A. M. Neculai, P. Yip and P. L. Howell

Synopsis: The expression, purification and crystallization of the alginate lyase AlgL from P. aeruginosa is described. The crystals diffracted to a resolution of 1.64 Å.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 588-590
[ doi:10.1107/S1744309112012341 ]

Crystallization and preliminary X-ray crystallographic analysis of hydroquinone dioxygenase from Sphingomonas sp. TTNP3

S. Da Vela, M. Ferraroni, B. A. Kolvenbach, E. Keller, P. F. X. Corvini, A. Scozzafava and F. Briganti

Synopsis: Crystals of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3 belonged to space group P2₁ and contained two heterotetramers in the asymmetric unit related by 222 noncrystallographic symmetry. X-ray data were collected to a maximum resolution of 2.5 Å using a synchrotron source.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 591-595
[ doi:10.1107/S1744309112011888 ]

Crystallization and preliminary characterization of chloromuconolactone dehalogenase from Rhodococcus opacus 1CP

C. Roth, S. R. Kaschabek, J. A. D. Gröning, T. Handrek, M. Schlömann and N. Sträter

Synopsis: The crystallization and preliminary X-ray diffraction analysis of a novel chloromuconolactone dehalogenase from R. opacus 1CP are described. The oligomeric state was determined based on the self-rotation function.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 596-600
[ doi:10.1107/S1744309112012912 ]

Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes

T. W. Geders, K. Gustafson and B. C. Finzel

Synopsis: The utility of differential scanning fluorimetry for homogeneity assessment and crystallization improvement of PLP-dependent enzymes is demonstrated using the potential drug target BioA from M. tuberculosis.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 601-605
[ doi:10.1107/S1744309112010172 ]

On-column ligand exchange for structure-based drug design: a case study with human 11-hydroxysteroid dehydrogenase type 1

W. Qin, R. A. Judge, K. L. Longenecker, L. R. Solomon and J. E. Harlan

Synopsis: An on-column ligand- and detergent-exchange method was developed to obtain ligand-protein complexes for an adamantane series of compounds with 11-HSD1 after a variety of other complexation methods had failed. An interesting byproduct of the method was the observation of artificial trimers in the crystal structures.

Online 21 April 2012

 

Acta Cryst. (2012). F68, 606-609
[ doi:10.1107/S174430911201161X ]

`Seeding' with protease to optimize protein crystallization conditions in in situ proteolysis

J. Huang, Y. Gong, D. Huang, L. Haire, J. Liu and Y. Peng

Synopsis: Addition of protease instead of seeds using a robot can be used to optimize the concentration of protease in in situ proteolysis experiments and has been successfully tested using two proteins.

Online 24 April 2012